Nano-Mg–Al-layered double hydroxide application to cotton for enhancing mechanical, UV protection and flame retardancy at low cytotoxicity level

Cellulose ◽  
2016 ◽  
Vol 24 (2) ◽  
pp. 1107-1120 ◽  
Author(s):  
Sunita Barik ◽  
Asimananda Khandual ◽  
Lingaraj Behera ◽  
Sushanta Kumar Badamali ◽  
Ameersing Luximon
Keyword(s):  
Layered Double Hydroxide ◽  
Flame Retardancy ◽  
Uv Protection ◽  
Low Cytotoxicity ◽  
Double Hydroxide

scholarly journals Layered Double Hydroxide as a Potent Non-viral Vector for Nucleic Acid Delivery Using Gene-Activated Scaffolds for Tissue Regeneration Applications

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1219
Author(s):  
Lara S. Costard ◽  
Domhnall C. Kelly ◽  
Rachael N. Power ◽  
Christopher Hobbs ◽  
Sonia Jaskaniec ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Layered Double Hydroxide ◽  
Viral Vectors ◽  
Viral Vector ◽  
Nucleic Acid Delivery ◽  
Safe Alternative ◽  
Transfection Efficacy ◽  
Mass Ratios ◽  
Low Cytotoxicity ◽  
Double Hydroxide

Nonviral vectors offer a safe alternative to viral vectors for gene therapy applications, albeit typically exhibiting lower transfection efficiencies. As a result, there remains a significant need for the development of a nonviral delivery system with low cytotoxicity and high transfection efficacy as a tool for safe and transient gene delivery. This study assesses MgAl-NO3 layered double hydroxide (LDH) as a nonviral vector to deliver nucleic acids (pDNA, miRNA and siRNA) to mesenchymal stromal cells (MSCs) in 2D culture and using a 3D tissue engineering scaffold approach. Nanoparticles were formulated by complexing LDH with pDNA, microRNA (miRNA) mimics and inhibitors, and siRNA at varying mass ratios of LDH:nucleic acid. In 2D monolayer, pDNA delivery demonstrated significant cytotoxicity issues, and low cellular transfection was deemed to be a result of the poor physicochemical properties of the LDH–pDNA nanoparticles. However, the lower mass ratios required to successfully complex with miRNA and siRNA cargo allowed for efficient delivery to MSCs. Furthermore, incorporation of LDH–miRNA nanoparticles into collagen-nanohydroxyapatite scaffolds resulted in successful overexpression of miRNA in MSCs, demonstrating the development of an efficacious miRNA delivery platform for gene therapy applications in regenerative medicine.

CELLULAR UPTAKE BEHAVIOR OF FLUORESCEIN: INTERCALATED LAYERED DOUBLE HYDROXIDE

2012 ◽  
Vol 05 (02) ◽  
pp. 1260003 ◽  
Author(s):  
MIYUKI TANAKA ◽  
SUMIO AISAWA ◽  
HIDETOSHI HIRAHARA ◽  
EIICHI NARITA ◽  
SHU YIN ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Hydrothermal Treatment ◽  
Layered Double Hydroxide ◽  
Mammalian Cell ◽  
Cellular Uptake ◽  
L929 Cell ◽  
Low Cytotoxicity ◽  
High Fluorescence ◽  
Double Hydroxide

In order to define the ability of layered double hydroxide (LDH) as materials for drug delivery, fluorescein (Fluo) anion intercalated LDH (Fluo/LDH) was synthesized by hydrothermal treatment and observed the cellular uptake of the Fluo/LDH for mammalian cell (L929). The synthesized Fluo/LDH showed a LDH structure, high fluorescence and low cytotoxicity. According to the fluorescence, confocal and TEM images of cells, the Fluo/LDH seemed to be internalized into the L929 cell by cellular endocytosis and dissolved inside the cell to exhibit the fluorescence of cellular cytoplasm.

scholarly journals Fabrication of Superhydrophobic Mg/Al Layered Double Hydroxide (LDH) Coatings on Medium Density Fiberboards (MDFs) with Flame Retardancy

Materials ◽  
2018 ◽  
Vol 11 (7) ◽  
pp. 1113 ◽  
Author(s):  
Zhe Wang ◽  
Xiaoping Shen ◽  
Temeng Qian ◽  
Kang Xu ◽  
Qingfeng Sun ◽  
...  
Keyword(s):  
Layered Double Hydroxide ◽  
Flame Retardancy ◽  
Medium Density ◽  
Double Hydroxide
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