Combination of hydrothermal pretreatment and sodium hydroxide post-treatment applied on wheat straw for enhancing its enzymatic hydrolysis

Cellulose ◽  
2017 ◽  
Vol 25 (2) ◽  
pp. 1197-1206 ◽  
Author(s):  
Douyong Min ◽  
Lei Wei ◽  
Ting Zhao ◽  
Mingfu Li ◽  
Zhuan Jia ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sodium Hydroxide ◽  
Wheat Straw ◽  
Hydrothermal Pretreatment ◽  
Post Treatment

scholarly journals Optimization of alkali pretreatment of wheat straw to be used as substrate for biofuels production  

2013 ◽  
Vol 59 (No. 12) ◽  
pp. 537-542 ◽  
Author(s):  
K. Jaisamut ◽  
L. Paulová ◽  
P. Patáková ◽  
M. Rychtera ◽  
K. Melzoch
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Sodium Hydroxide ◽  
Wheat Straw ◽  
Response Surface ◽  
Fermentable Sugars ◽  
Alkali Pretreatment ◽  
The Media ◽  
Pretreatment Conditions ◽  
Expert Designer

Alkali pretreatment of wheat straw was optimized by response surface methodology to maximize yields of fermentable sugars in subsequent enzymatic hydrolysis and to remove maximum lignin in order to improve rheological attributes of the media. The effects of pretreatment conditions on biomass properties were studied using the Expert Designer software. Concentration of sodium hydroxide and temperature were the factors most affecting pretreatment efficiency. At the optimum (80°C, 39 min, 0.18 g NaOH and 0.06 g lime per g of raw biomass), 93.1 ± 1.0% conversion of cellulose to glucose after enzymatic hydrolysis and 80.3 ± 1.2% yield of monosaccharides (glucose plus xylose and arabinose) from cellulose and hemicellulose of wheat straw were achieved.

scholarly journals Optimization of Sodium Hydroxide Pretreatment and Enzymatic Hydrolysis of Wheat Straw Powder by Cellulases and Xylanases From Aspergillus Niger HQ-1 Using Response Surface Methodology

2021 ◽  
Author(s):  
Hui Zhang ◽  
Junhui Wu
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Sodium Hydroxide ◽  
Wheat Straw ◽  
Response Surface ◽  
Sodium Hydroxide Solution ◽  
Solid Loading ◽  
Enzyme Loading ◽  
Hydrolysis Of

Abstract To maximize fermentable sugars production, response surface methodology (RSM) was adopted to optimize pretreatment and enzymatic hydrolysis of wheat straw powder (WSP) using the crude cellulases preparation containing xylanases from Aspergillus niger HQ-1. Factors of pretreatment including sodium hydroxide concentration, pretreatment time and temperature were found to have significant effects on sugars production. Results indicated that WSP with particle size 0.3 mm should be pretreated using 1.8% (w/v) sodium hydroxide solution with 25.0% (w/v) of solid loading at 94.0°C for 46.0 min and the optimized pretreatment conditions could result in 90.9% of cellulose recovery, 54.6% of hemicellulose recovery and 72.7% of lignin removal, respectively. Furthermore, variables of enzymatic hydrolysis including enzyme loading, biomass loading and reaction time were proved to have significant effects on sugars yields. After hydrolysis at 50°C for 44.8 h with 7.1% (w/v) of biomass loading, 8.1 FPU/g of enzyme loading and 0.2% (w/v) of Tween-80, maximum yields of reducing sugar (632.92 mg/g) and xylose (149.83 mg/g) could be obtained, respectively. In addition, holocellulose and hemicellulose conversion were 81.6% and 80.0%, respectively. To the best of our knowledge, this is the first report about systematic optimization of sodium hydroxide pretreatment and enzymatic hydrolysis of WSP using RSM.

Two-Dimensional NMR Evidence for Cleavage of Lignin and Xylan Substituents in Wheat Straw Through Hydrothermal Pretreatment and Enzymatic Hydrolysis

2012 ◽  
Vol 6 (1) ◽  
pp. 211-221 ◽  
Author(s):  
Daniel J. Yelle ◽  
Prasad Kaparaju ◽  
Christopher G. Hunt ◽  
Kolby Hirth ◽  
Hoon Kim ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Hydrothermal Pretreatment ◽  
Two Dimensional

Pretreatment of wheat straw by supercritical CO2 and its enzymatic hydrolysis for sugar production

2010 ◽  
Vol 107 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Roozbeh Alinia ◽  
Samyar Zabihi ◽  
Feridun Esmaeilzadeh ◽  
Jamshid Fathi Kalajahi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Supercritical Co2 ◽  
Sugar Production

scholarly journals Hydrothermal Pretreatment of Wheat Straw: Effects of Temperature and Acidity on Byproduct Formation and Inhibition of Enzymatic Hydrolysis and Ethanolic Fermentation

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 487
Author(s):  
Dimitrios Ilanidis ◽  
Stefan Stagge ◽  
Leif J. Jönsson ◽  
Carlos Martín
Keyword(s):  
Mass Spectrometry ◽  
Sulfuric Acid ◽  
Wheat Straw ◽  
High Performance ◽  
Enzymatic Saccharification ◽  
Hydrothermal Pretreatment ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzymatic Digestibility ◽  
Acid Catalyzed ◽  
Pretreatment Conditions

Biochemical conversion of wheat straw was investigated using hydrothermal pretreatment, enzymatic saccharification, and microbial fermentation. Pretreatment conditions that were compared included autocatalyzed hydrothermal pretreatment at 160, 175, 190, and 205 °C and sulfuric-acid-catalyzed hydrothermal pretreatment at 160 and 190 °C. The effects of using different pretreatment conditions were investigated with regard to (i) chemical composition and enzymatic digestibility of pretreated solids, (ii) carbohydrate composition of pretreatment liquids, (iii) inhibitory byproducts in pretreatment liquids, (iv) furfural in condensates, and (v) fermentability using yeast. The methods used included two-step analytical acid hydrolysis combined with high-performance anion-exchange chromatography (HPAEC), HPLC, ultra-high performance liquid chromatography-electrospray ionization-triple quadrupole-mass spectrometry (UHPLC-ESI-QqQ-MS), and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). Lignin recoveries in the range of 108–119% for autocatalyzed hydrothermal pretreatment at 205 °C and sulfuric-acid-catalyzed hydrothermal pretreatment were attributed to pseudolignin formation. Xylose concentration in the pretreatment liquid increased with temperature up to 190 °C and then decreased. Enzymatic digestibility was correlated with the removal of hemicelluloses, which was almost quantitative for the autocatalyzed hydrothermal pretreatment at 205 °C. Except for the pretreatment liquid from the autocatalyzed hydrothermal pretreatment at 205 °C, the inhibitory effects on Saccharomyces cerevisiae yeast were low. The highest combined yield of glucose and xylose was achieved for autocatalyzed hydrothermal pretreatment at 190 °C and the subsequent enzymatic saccharification that resulted in approximately 480 kg/ton (dry weight) raw wheat straw.

Kinetic model of enzymatic hydrolysis of steam-exploded wheat straw

2012 ◽  
Vol 87 (2) ◽  
pp. 1280-1285 ◽  
Author(s):  
Greta Radeva ◽  
Ivo Valchev ◽  
Stoiko Petrin ◽  
Eva Valcheva ◽  
Petya Tsekova
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Model ◽  
Wheat Straw ◽  
Hydrolysis Of
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