Overproduction of fungal endo-β-1,4-glucanase leads to characteristic lignocellulose modification for considerably enhanced biomass enzymatic saccharification and bioethanol production in transgenic rice straw

Cellulose ◽  
2019 ◽  
Vol 26 (15) ◽  
pp. 8249-8261 ◽  
Author(s):  
Ying Li ◽  
Haiyan Sun ◽  
Chunfen Fan ◽  
Huizhen Hu ◽  
Leiming Wu ◽  
...  
2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.


2018 ◽  
Vol 20 (9) ◽  
pp. 2047-2056 ◽  
Author(s):  
Ying Li ◽  
Peng Liu ◽  
Jiangfeng Huang ◽  
Ran Zhang ◽  
Zhen Hu ◽  
...  

A cost-effective biomass conversion technology for high bioethanol production under mild chemical pretreatment in the transgenic rice straw.


Fuel ◽  
2021 ◽  
Vol 301 ◽  
pp. 121074
Author(s):  
Nisha Singh ◽  
Ravi P. Gupta ◽  
Suresh K. Puri ◽  
Anshu S. Mathur

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3870
Author(s):  
Jingyang Li ◽  
Fei Liu ◽  
Hua Yu ◽  
Yuqi Li ◽  
Shiguang Zhou ◽  
...  

Banana is a major fruit crop throughout the world with abundant lignocellulose in the pseudostem and rachis residues for biofuel production. In this study, we collected a total of 11 pseudostems and rachis samples that were originally derived from different genetic types and ecological locations of banana crops and then examined largely varied edible carbohydrates (soluble sugars, starch) and lignocellulose compositions. By performing chemical (H2SO4, NaOH) and liquid hot water (LHW) pretreatments, we also found a remarkable variation in biomass enzymatic saccharification and bioethanol production among all banana samples examined. Consequently, this study identified a desirable banana (Refen1, subgroup Pisang Awak) crop containing large amounts of edible carbohydrates and completely digestible lignocellulose, which could be combined to achieve the highest bioethanol yields of 31–38% (% dry matter), compared with previously reported ones in other bioenergy crops. Chemical analysis further indicated that the cellulose CrI and lignin G-monomer should be two major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis. Therefore, this study not only examined rich edible carbohydrates for food in the banana pseudostems but also detected digestible lignocellulose for bioethanol production in rachis tissue, providing a strategy applicable for genetic breeding and biomass processing in banana crops.


2017 ◽  
Vol 29 (6) ◽  
pp. 3201-3209 ◽  
Author(s):  
Maria Dyah Nur Meinita ◽  
Bintang Marhaeni ◽  
Yong-Ki Hong ◽  
Gwi-Taek Jeong

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