Mild chemical pretreatments are sufficient for bioethanol production in transgenic rice straws overproducing glucosidase

2018 ◽  
Vol 20 (9) ◽  
pp. 2047-2056 ◽  
Author(s):  
Ying Li ◽  
Peng Liu ◽  
Jiangfeng Huang ◽  
Ran Zhang ◽  
Zhen Hu ◽  
...  
Keyword(s):  
Rice Straw ◽  
Transgenic Rice ◽  
Biomass Conversion ◽  
Cost Effective ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Chemical Pretreatments ◽  
Conversion Technology

A cost-effective biomass conversion technology for high bioethanol production under mild chemical pretreatment in the transgenic rice straw.

Bioethanol Production from Rice Straw in the MAFF Research Project, Japan

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu
Keyword(s):  
Heat Treatment ◽  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Simple Method ◽  
Herbaceous Biomass ◽  
Free Glucose ◽  
Simultaneous Saccharification

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.

Process optimization for chemical pretreatment of rice straw for bioethanol production

2020 ◽  
Vol 156 ◽  
pp. 1233-1243 ◽  
Author(s):  
Anu ◽  
Anil Kumar ◽  
Kavish Kumar Jain ◽  
Bijender Singh
Keyword(s):  
Rice Straw ◽  
Process Optimization ◽  
Bioethanol Production ◽  
Chemical Pretreatment

scholarly journals Batch fermentation and Simultaneous Saccharification and Fermentation (SSF) processes by Meyerozyma Guilliermondii Strain F22 and Saccharomyces cerecvisae for xylitol and bioethanol co-production

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Dr.Fawzia Shalsh ◽  
Dhoha Kadeem Nagimm ◽  
Muhammad Abdul Alrheem ◽  
Saffa Abedul Alrheem
Keyword(s):  
Rice Straw ◽  
Simultaneous Saccharification And Fermentation ◽  
Process Integration ◽  
Solid Phase ◽  
Cost Effective ◽  
Product Yield ◽  
Economic Benefits ◽  
Meyerozyma Guilliermondii ◽  
Chemical Pretreatments ◽  
Simultaneous Saccharification

Recent years have seen an increase in the use of lignocellulosic materials in the development of bioproduct, biorefinery technologies have focused on process integration for the production of different valuable coproducts in order to reduce the overall processing cost. In this study, agricultural wastes from rice straw were used for the co-production of bioethanol and xylitol. Where bioethanol is produced from the cellulosic fraction and xylitol from the hemicellulose fraction after elimination of lignin using chemical pretreatments. The chemical treatment was carried out with diluted acid 2.5% at a 100 °C for 30 minutes , and then exposed the cellulosic fraction of the solid phase resulting from the chemical process to the enzymatic action of the fungus Trichoderma harzianum for releas sugars and fermented at a later stage using  Saccharomyces cerecvisae for bioethanol production in a simultaneous saccharification and fermentation process, The liquid phase hemicellulose fraction  was exposed to action of Meyerozyma guilliermondii strain F22 (Pichia guilliermondii) for xylitol production. Resulting was accomplished yielding maximum concentrations and product yield were 32.6 g/L 0.39g/g and 20.1 g/L, 0.44g/g for bioethanol and xylitol respectively of the total glucose and xylose available in rice straw, the co-production of xylitol with ethanol in an integrated biorefinery would create economic benefits making the overall lignocellulose-based process more cost effective

Revalorizing Lignocellulose for the Production of Natural Pharmaceuticals and Other High Value Bioproducts

2019 ◽  
Vol 26 (14) ◽  
pp. 2475-2484 ◽  
Author(s):  
Congqiang Zhang ◽  
Heng-Phon Too
Keyword(s):  
Clostridium Thermocellum ◽  
White Rot Fungi ◽  
Brown Rot ◽  
Cost Effective ◽  
White Rot ◽  
Chemical Pretreatment ◽  
Significant Challenge ◽  
Drug Molecules ◽  
Natural Medicine ◽  
Renewable Natural Resource

Lignocellulose is the most abundant renewable natural resource on earth and has been successfully used for the production of biofuels. A significant challenge is to develop cost-effective, environmentally friendly and efficient processes for the conversion of lignocellulose materials into suitable substrates for biotransformation. A number of approaches have been explored to convert lignocellulose into sugars, e.g. combining chemical pretreatment and enzymatic hydrolysis. In nature, there are organisms that can transform the complex lignocellulose efficiently, such as wood-degrading fungi (brown rot and white rot fungi), bacteria (e.g. Clostridium thermocellum), arthropods (e.g. termite) and certain animals (e.g. ruminant). Here, we highlight recent case studies of the natural degraders and the mechanisms involved, providing new utilities in biotechnology. The sugars produced from such biotransformations can be used in metabolic engineering and synthetic biology for the complete biosynthesis of natural medicine. The unique opportunities in using lignocellulose directly to produce natural drug molecules with either using mushroom and/or ‘industrial workhorse’ organisms (Escherichia coli and Saccharomyces cerevisiae) will be discussed.

Bioethanol production from pretreated whole slurry rice straw by thermophilic co-culture

Fuel ◽  
2021 ◽  
Vol 301 ◽  
pp. 121074
Author(s):  
Nisha Singh ◽  
Ravi P. Gupta ◽  
Suresh K. Puri ◽  
Anshu S. Mathur
Keyword(s):  
Rice Straw ◽  
Bioethanol Production ◽  
Whole Slurry

scholarly journals Effects of Rice Straw Powder (RSP) Size and Pretreatment on Properties of FDM 3D-Printed RSP/Poly(Lactic Acid) Biocomposites

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3234
Author(s):  
Wangwang Yu ◽  
Lili Dong ◽  
Wen Lei ◽  
Yuhan Zhou ◽  
Yongzhe Pu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rice Straw ◽  
Fused Deposition Modeling ◽  
Flexural Modulus ◽  
Tensile Modulus ◽  
Cost Effective ◽  
Poly Lactic Acid ◽  
Fused Deposition ◽  
Interfacial Compatibility ◽  
3D Printed

To develop a new kind of environment-friendly composite filament for fused deposition modeling (FDM) 3D printing, rice straw powder (RSP)/poly(lactic acid) (PLA) biocomposites were FDM-3D-printed, and the effects of the particle size and pretreatment of RSP on the properties of RSP/PLA biocomposites were investigated. The results indicated that the 120-mesh RSP/PLA biocomposites (named 120#RSP/PLA) showed better performance than RSP/PLA biocomposites prepared with other RSP sizes. Infrared results showed that pretreatment of RSP by different methods was successful, and scanning electron microscopy indicated that composites prepared after pretreatment exhibited good interfacial compatibility due to a preferable binding force between fiber and matrix. When RSP was synergistically pretreated by alkaline and ultrasound, the composite exhibited a high tensile strength, tensile modulus, flexural strength, and flexural modulus of 58.59, 568.68, 90.32, and 3218.12 MPa, respectively, reflecting an increase of 31.19%, 16.48%, 18.75%, and 25.27%, respectively, compared with unmodified 120#RSP/PLA. Pretreatment of RSP also improved the thermal stability and hydrophobic properties, while reducing the water absorption of 120#RSP/PLA. This work is believed to provide highlights of the development of cost-effective biocomposite filaments and improvement of the properties of FDM parts.

scholarly journals Chemical Pretreatment-Independent Saccharifications of Xylan and Cellulose of Rice Straw by Bacterial Weak Lignin-Binding Xylanolytic and Cellulolytic Enzymes

2017 ◽  
Vol 83 (22) ◽  
Author(s):  
Thitiporn Teeravivattanakit ◽  
Sirilak Baramee ◽  
Paripok Phitsuwan ◽  
Somphit Sornyotha ◽  
Rattiya Waeonukul ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
Chemical Pretreatment ◽  
Environment Friendly ◽  
Glucose Yield ◽  
Content Type ◽  
Enzyme Pretreatment ◽  
One Step ◽  
Arabinoxylan Arabinofuranohydrolase ◽  
Xylanolytic Enzyme

ABSTRACT Complete utilization of carbohydrate fractions is one of the prerequisites for obtaining economically favorable lignocellulosic biomass conversion. This study shows that xylan in untreated rice straw was saccharified to xylose in one step without chemical pretreatment, yielding 58.2% of the theoretically maximum value by Paenibacillus curdlanolyticus B-6 PcAxy43A, a weak lignin-binding trifunctional xylanolytic enzyme, endoxylanase/β-xylosidase/arabinoxylan arabinofuranohydrolase. Moreover, xylose yield from untreated rice straw was enhanced to 78.9% by adding endoxylanases PcXyn10C and PcXyn11A from the same bacterium, resulting in improvement of cellulose accessibility to cellulolytic enzyme. After autoclaving the xylanolytic enzyme-treated rice straw, it was subjected to subsequent saccharification by a combination of the Clostridium thermocellum endoglucanase CtCel9R and Thermoanaerobacter brockii β-glucosidase TbCglT, yielding 88.5% of the maximum glucose yield, which was higher than the glucose yield obtained from ammonia-treated rice straw saccharification (59.6%). Moreover, this work presents a new environment-friendly xylanolytic enzyme pretreatment for beneficial hydrolysis of xylan in various agricultural residues, such as rice straw and corn hull. It not only could improve cellulose saccharification but also produced xylose, leading to an improvement of the overall fermentable sugar yields without chemical pretreatment. IMPORTANCE Ongoing research is focused on improving “green” pretreatment technologies in order to reduce energy demands and environmental impact and to develop an economically feasible biorefinery. The present study showed that PcAxy43A, a weak lignin-binding trifunctional xylanolytic enzyme, endoxylanase/β-xylosidase/arabinoxylan arabinofuranohydrolase from P. curdlanolyticus B-6, was capable of conversion of xylan in lignocellulosic biomass such as untreated rice straw to xylose in one step without chemical pretreatment. It demonstrates efficient synergism with endoxylanases PcXyn10C and PcXyn11A to depolymerize xylan in untreated rice straw and enhanced the xylose production and improved cellulose hydrolysis. Therefore, it can be considered an enzymatic pretreatment. Furthermore, the studies here show that glucose yield released from steam- and xylanolytic enzyme-treated rice straw by the combination of CtCel9R and TbCglT was higher than the glucose yield obtained from ammonia-treated rice straw saccharification. This work presents a novel environment-friendly xylanolytic enzyme pretreatment not only as a green pretreatment but also as an economically feasible biorefinery method.

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