Cissus quadrangularis stem derived fiber: a natural osteoinductive substrate for regenerative bone tissue engineering

Cellulose ◽  
2021 ◽  
Author(s):  
Praseetha R. Nair ◽  
S. Sreeja ◽  
G. S. Sailaja
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cissus Quadrangularis

scholarly journals Layer-by-layer decorated herbal cell compatible scaffolds for bone tissue engineering: A synergistic effect of graphene oxide and Cissus quadrangularis

2020 ◽  
Vol 35 (1) ◽  
pp. 57-73 ◽  
Author(s):  
Shivaji Kashte ◽  
RK Sharma ◽  
Sachin Kadam
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Synergistic Effect ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cellular Proliferation ◽  
Human Umbilical Cord ◽  
Layer By Layer ◽  
Cissus Quadrangularis

Among various bone regenerative and repair methods, use of osteoinductive scaffold as bone grafts/substitute has gained wide importance worldwide. To develop such osteoinductive scaffold that is more natural and which spontaneously stimulates osteoblast formation without any differentiation media, we prepared electrospun poly ε-caprolactone scaffold which is further modified by means of layer-by-layer method using Cissus quadrangularis callus culture extract and graphene oxide (PCL-GO-CQ). The modified PCL-GO-CQ scaffold was compared with plain poly ε-caprolactone scaffold and poly ε-caprolactone coated only with graphene oxide. Physical properties, such as roughness, wettability, yield strength and tensile strength, of PCL-GO-CQ scaffold were found to be superior. Also, PCL-GO-CQ scaffold showed more in vitro cell compatibility with enhanced cellular proliferation on its surface. Presence of graphene oxide and Cissus quadrangularis callus in scaffold helped in the differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into osteogenic lineage without any differentiation media in less than 20 days. The synergistic effect of Cissus quadrangularis callus extract and graphene oxide in PCL-GO-CQ scaffold enhanced osteoblastic differentiation, osteoconduction and osteoinduction potential of scaffolds making them highly potential in bone regeneration and bone tissue engineering applications.

scholarly journals Layer by layer deposited osteoinductive scaffolds for bone tissue engineering

2020 ◽  
Vol 9 (2) ◽  
pp. 1089-1098
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Layer By Layer ◽  
Cissus Quadrangularis ◽  
Culture Extract

There are various bone regenerative and repair methods, but the use of osteoinductive scaffolds as bone grafts/substitute has gained wide importance worldwide. Here, an osteoinductive scaffold is developed which spontaneously stimulates stem cells to osteoblast formation without the use of any growth factor or differentiation media. We prepared electrospun PCL scaffold which is further modified for osteoinductivity by layer-by-layer method using graphene and Cissus quadrangularis callus culture extract (PCL-GP-CQ). The modified PCL-GP-CQ scaffold was compared with plain PCL scaffold and PCL coated only with GP. Physical properties such as roughness, wettability, yield strength and tensile strength of PCL-GP-CQ scaffold were found to be superior. Also, PCL-GP-CQ scaffold when seeded with human umbilical cord Wharton's jelly derived mesenchymal stem cells showed higher in vitro biocompatibility with enhanced cellular proliferation on its surface. Synergistic effect of graphene and Cissus Quadrangularis callus culture extract in scaffold boosted the differentiation of human Umbilical Cord Wharton’s jelly derived Mesenchymal Stem Cells into osteogenic lineage without any differentiation media in less than 20 days. The PCL-GP-CQ scaffold enhanced osteoblastic differentiation, osteoconduction and osteoinduction potential of scaffolds making them highly suitable for bone regeneration and bone tissue engineering applications.

Development of a phytochemical scaffold for bone tissue engineering using Cissus quadrangularis extract

2012 ◽  
Vol 87 (2) ◽  
pp. 1787-1795 ◽  
Author(s):  
S. Soumya ◽  
K.M. Sajesh ◽  
R. Jayakumar ◽  
S.V. Nair ◽  
K.P. Chennazhi
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cissus Quadrangularis

Early Biomineralizing Chitosan-Collagen Hybrid Scaffold with Cissus quadrangularis Extract for Regenerative Bone Tissue Engineering

2021 ◽  
Author(s):  
Praseetha R Nair ◽  
Sreeja S ◽  
G.S. Sailaja
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stability ◽  
Hexane Extract ◽  
Hybrid Scaffold ◽  
Biodegradable Scaffold ◽  
Cissus Quadrangularis ◽  
Porous Chitosan ◽  
Cross Linker

This study demonstrates the strategic fabrication of Cissus quadrangularis (CQ) hexane extract integrated porous chitosan-collagen (CH-CO-HE) biodegradable scaffold crosslinked with glyoxal, a biocompatible cross-linker, that enables sufficient mechanical stability and...

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

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