scholarly journals Considerations on the Toxicity of Brilliant Blue FCF Aqueous Solutions before and after Ozonation

2020 ◽  
Vol 71 (4) ◽  
pp. 356-365
Author(s):  
Maria Marcvart Tiron ◽  
Irina Eugenia Lucaciu ◽  
Mihai Nita-Lazar ◽  
Stefania Gheorghe
Keyword(s):  
Toxic Effect ◽  
Aquatic Organisms ◽  
Brilliant Blue ◽  
Ozone Treatment ◽  
Brilliant Blue Fcf ◽  
Food Dyes ◽  
Before And After ◽  
Effect Level ◽  
Ozonation Process ◽  
By Products

The food dyes have a potential toxic effect on aquatic organisms which trigger the necessity of their removal from industrial or urban wastewaters. Many different removal methods were investigated for this purpose, but the ozonation and advanced oxidation processes (AOPs) were successfully applied in this field. However, the majority of studies emphasized that color removal by ozonation process did not report a complete mineralization of the dye and the resulted by-products may have a greater toxicity than the original compound. In this context, the paper presents a comparative ecotoxicity study of the dye Brilliant Blue FCF (BB FCF) before and after ozone treatment. The BB FCF toxic effect, before and after ozonation was investigated on crustacean (Daphnia magna), lethal or inhibitory concentrations for 50% of tested organisms (LC50 / EC50) were used to estimate the effect level. The dye showed no toxicity on crustacean (CL50/CE50]100mg/L) before ozonation. The ozonized solutions presented a high toxicity for crustaceans compared to initial dye due to the by-products occurrence.

scholarly journals Food dyes as an alternative tracking dye for DNA gel electrophoresis

2013 ◽  
Vol 10 (4) ◽  
pp. 1150-1156 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Uv Light ◽  
Brilliant Blue ◽  
Local Market ◽  
Synthetic Dyes ◽  
Sunset Yellow ◽  
Dna Ladder ◽  
Food Dye ◽  
Brilliant Blue Fcf ◽  
Wide Range ◽  
Food Dyes

The chemical, physical and toxicological effects on health of synthetic dyes that used as tracking dye in the electrophoresis requires seriously search about alternative tracking dye. The present study is aimed to find an alternative dye from safe food dyes which commonly used in food coloring. Five dyes were selected depending on their chemical properties and the availability in local market: Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF, Carmoisine, and green traditional, three dyes were chosen to be mixed as loading buffer: Brilliant Blue FCF, Sunset Yellow FCF as a basic because it give the whole range size of most traditional loading buffers that available in market, and adding the Carmoisine as a new indicator for the bands less than 50bp, then mixed with DNA ladder in same percentage used with traditional loading buffers to clarify the effects of dyes on DNA, migrated on 1% agarose with loading buffer promega, results showed more clarity and highly readable separation of dyes and give wide range of size in the food loading mix than promega loading dye, by viewing the gel on UV light the DNA ladder were moved smoothly, bands separated effeminately on gel and in same rate of the DNA ladder that load with promega loading buffer which indicate no interaction between the food dyes and the DNA.Our studies show that the food dye can be used as a tracking dye in place of used synthetic dye. The procedure is found to be easy, practical, safely and reliable.

scholarly journals Sustainable Sources from Aquatic Organisms for Cosmeceuticals Ingredients

Cosmetics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 48
Author(s):  
Carmen G. Sotelo ◽  
María Blanco ◽  
Patricia Ramos ◽  
José A. Vázquez ◽  
Ricardo I. Perez-Martin
Keyword(s):  
Social Issues ◽  
Good Condition ◽  
Aquatic Organisms ◽  
The Body ◽  
Fishing Activity ◽  
Marine Biomass ◽  
Cosmetic Industry ◽  
Wide Range ◽  
Long Life ◽  
By Products

Long life expectancy of populations in the developing world together with some cultural and social issues has driven the need to pay special attention to health and physical appearance. Cosmeceuticals are gaining interest in the cosmetic industry as their uses fulfills a double purpose: the requirements of a cosmetic (clean, perfume, protect, change the appearance of the external parts of the body or keeping them in good condition) with a particular bioactivity function. The cosmetics industry, producing both cosmetics and cosmeceuticals, is currently facing numerous challenges to satisfy different attitudes of consumers (vegetarianism, veganism, cultural or religious concerns, health or safety reasons, eco-friendly process, etc.). A currently growing trend in the market is the interest in products of low environmental impact. Marine origin ingredients are increasingly being incorporated into cosmeceutical preparations because they are able to address several consumer requirements and also due to the wide range of bioactivities they present (antioxidant, whitening, anti-aging, etc.). Many companies claim “Marine” as a distinctive marketing signal; however, only a few indicate whether they use sustainable ingredient sources. Sustainable marine ingredients might be obtained using wild marine biomass through a sustainable extractive fishing activity; by adopting valorization strategies including the use of fish discards and fish by-products; and by sustainably farming and culturing marine organisms.

scholarly journals Effect of Ozone Treatment on the Quality of Sea Buckthorn (Hippophae rhamnoides L.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 847
Author(s):  
Anita Zapałowska ◽  
Natalia Matłok ◽  
Miłosz Zardzewiały ◽  
Tomasz Piechowiak ◽  
Maciej Balawejder
Keyword(s):  
Mechanical Properties ◽  
Volatile Compounds ◽  
Sea Buckthorn ◽  
Hippophae Rhamnoides ◽  
Ozone Treatment ◽  
Aerobic Bacteria ◽  
Hippophaë Rhamnoides ◽  
Hippophae Rhamnoïdes L ◽  
Ozonation Process

The aim of this research was to show the effect of the ozonation process on the quality of sea buckthorn (Hippophae rhamnoides L.). The quality of the ozonated berries of sea buckthorn was assessed. Prior to and after the ozone treatment, a number of parameters, including the mechanical properties, moisture content, microbial load, content of bioactive compounds, and composition of volatile compounds, were determined. The influence of the ozonation process on the composition of volatile compounds and mechanical properties was demonstrated. The ozonation had negligible impact on the weight and moisture of the samples immediately following the treatment. Significant differences in water content were recorded after 7 days of storage. It was shown that the highest dose of ozone (concentration and process time) amounting to 100 ppm for 30 min significantly reduced the water loss. The microbiological analyses showed the effect of ozone on the total count of aerobic bacteria, yeast, and mold. The applied process conditions resulted in the reduction of the number of aerobic bacteria colonies by 3 log cfu g−1 compared to the control (non-ozonated) sample, whereas the number of yeast and mold colonies decreased by 1 log cfu g−1 after the application of 100 ppm ozone gas for 30 min. As a consequence, ozone treatment enhanced the plant quality and extended plant’s storage life.

Hazardous impact of vinasse from distilled winemaking by-products in terrestrial plants and aquatic organisms

2019 ◽  
Vol 183 ◽  
pp. 109493 ◽  
Author(s):  
Rose Marie O.F. Sousa ◽  
Carla Amaral ◽  
Joana M.C. Fernandes ◽  
Irene Fraga ◽  
Sabrina Semitela ◽  
...  
Keyword(s):  
Aquatic Organisms ◽  
Terrestrial Plants ◽  
By Products

Evaluating Differences in Transport Behavior of Sodium Chloride and Brilliant Blue FCF in Sand Columns

2015 ◽  
Vol 109 (3) ◽  
pp. 765-779 ◽  
Author(s):  
Jiazhong Qian ◽  
Yanan Wu ◽  
Yong Zhang ◽  
Yong Liu ◽  
Yuehan Lu ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Brilliant Blue ◽  
Transport Behavior ◽  
Brilliant Blue Fcf

scholarly journals Brilliant blue FCF is a nontoxic dye for saphenous vein graft marking that abrogates response to injury

2016 ◽  
Vol 64 (1) ◽  
pp. 210-218 ◽  
Author(s):  
Kyle M. Hocking ◽  
Weifeng Luo ◽  
Fan Dong Li ◽  
Padmini Komalavilas ◽  
Colleen Brophy ◽  
...  
Keyword(s):  
Saphenous Vein ◽  
Vein Graft ◽  
Saphenous Vein Graft ◽  
Brilliant Blue ◽  
Brilliant Blue Fcf

scholarly journals Active transport of brilliant blue FCF across the Drosophila midgut and Malpighian tubule epithelia

2019 ◽  
Author(s):  
Dawson B.H. Livingston ◽  
Hirva Patel ◽  
Andrew Donini ◽  
Heath A. MacMillan
Keyword(s):  
Traumatic Injury ◽  
Malpighian Tubule ◽  
Malpighian Tubules ◽  
Brilliant Blue ◽  
Barrier Dysfunction ◽  
Concentration Gradients ◽  
Barrier Disruption ◽  
Brilliant Blue Fcf ◽  
Summary Statement

AbstractUnder conditions of stress, many animals suffer from epithelial barrier disruption that can cause molecules to leak down their concentration gradients, potentially causing a loss of organismal homeostasis, further injury or death. Drosophila is a common insect model, used to study barrier disruption related to aging, traumatic injury, or environmental stress. Net leak of a non-toxic dye (Brilliant blue FCF) from the gut lumen to the hemolymph is often used to identify barrier failure under these conditions, but Drosophila are capable of actively transporting structurally-similar compounds. Here, we examined whether cold stress (like other stresses) causes Brilliant blue FCF (BB-FCF) to appear in the hemolymph of flies fed the dye, and if so whether Drosophila are capable of clearing this dye from their body following chilling. Using in situ midgut leak and transport assays as well as Ramsay assays of Malpighian tubule transport, we tested whether these ionoregulatory epithelia can actively transport BB-FCF. In doing so, we found that the Drosophila midgut and Malpighian tubules can mobilize BB-FCF via an active transcellular pathway, suggesting that elevated concentrations of the dye in the hemolymph may occur from increased paracellular permeability, reduced transcellular clearance, or both.Summary StatementDrosophila are able to actively secrete Brilliant blue FCF, a commonly used marker of barrier dysfunction

scholarly journals Assessment of Bacteriological Quality of Sheep Carcasses, Effect Level of 2.5% Citric Acid Spray on Bacterial Contamination of Meat, and Hygiene Practices of Workers in a Selected Abattoir in Debrezeit Town, Central Ethiopia

2021 ◽  
Vol 15 ◽  
pp. 117863022110375
Author(s):  
Derebew Aynewa ◽  
Zemichael Gizaw ◽  
Aklilu Feleke Haile
Keyword(s):  
Citric Acid ◽  
Bacterial Contamination ◽  
Total Coliform ◽  
Aerobic Bacteria ◽  
Bacteriological Quality ◽  
Before And After ◽  
Effect Level ◽  
The Mean ◽  
Structured Questionnaire

Background: Meat safety is important for public health. As part of the meat chain abattoirs are required to give attention to meat hygiene and safety in order to minimize hazards. Therefore, the current study was conducted to evaluate the bacteriological quality of sheep carcasses, knowledge and hygienic practices of workers in a selected abattoir and to determine the effect level of 2.5% citric acid spray on total coliforms and aerobic bacteria load of raw sheep carcasses surfaces. Methods: A cross-sectional study design with structured questionnaire and observational checklists observation were used. A systematic random sampling technique was employed. A total of 50 sample swabs (25 swabs before citric acid spray and 25 after citric acid spray) were randomly taken from brisket, flank and rump of sheep’s carcasses. Swabs were moistened with buffered peptone water (BPW) and samples were taken by rubbing 100 cm2 (10 cm × 10 cm) area delineated by sterile aluminum template. In addition, we administered a structured questionnaire and an observational checklists to assess knowledge and hygienic practices of workers. Bacteriological quality of sheep carcasses were analyzed using the methods described by the US bacteriological analytical manual. Results: The mean count for aerobic bacteria of the sheep carcasses before and after citric acid spray were 7.2log10 CFU/ml and 6.4log10 CFU/ml, respectively. The test results also showed that 21 (84%) and 15 (60%) of the swab samples were positive before and after spraying citric acid, respectively. The mean counts for coliform bacterial of the sheep carcasses before and after citric acid spray were 3.5log10 CFU/ml and 2.9log10 CFU/ml, respectively. The mean total aerobic and coliform counts before and after citric acid spray were significantly different ( P < .05). Regarding the hygiene condition of workers, all the respondents reported that they always washed their hands with soap before and after entering the slaughtering room and 23 (53.5%) of the workers reported that they used hot water. Thirty-one (72.1%) of the workers reported that they do not used soap to wash hands after visiting toilet. Thirty-five (81.4%) of the production workers did not wear mouth mask while handling and distribute meat/carcass. On the other hand, all of the workers wore capes, gowns and boots at the time of the observation and only 18 (18.6%) of the production workers wore gloves at the time of the survey. Conclusion: The current study revealed that significant proportion of sheep carcasses were positive for total aerobic bacteria and total coliform. Moreover, the study also showed that spraying of sheep carcasses with 2.5% citric acid significantly reduced the total coliform and aerobic counts. However, we did not assessed how much spray results to this effect. Therefore, we recommended further studies to determine how much spray of 2.5% citric acid significantly reduce bacterial contamination of sheep carcasses. In addition, the abattoir has to follow the food hazard analysis critical control point (HACCP) system to minimize meat contamination during harvesting and processing. The abattoir has to also implement strict operation laws to improve hygiene conditions of the workers. In addition, the abattoir can minimize meat contamination using 2.5% citric acid as a decontaminant.

scholarly journals UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

2018 ◽  
Vol 7 (2) ◽  
pp. 125-139
Author(s):  
Thais Nogueira Gonzaga ◽  
Dora Inés Kozusny-Andreani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Health Care Services ◽  
Pathogenic Bacteria ◽  
Ozone Treatment ◽  
Microbiological Analysis ◽  
Before And After ◽  
Staphylococcus Spp

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.Palavras-chave: Bactérias patogênicas. Fungos. Ozonização. USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.Keywords: Pathogenic bacteria. Fungi. Ozonization.

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