Mapping of maternal QTLs for in vivo haploid induction rate in maize (Zea mays L.)

Euphytica ◽  
2013 ◽  
Vol 196 (3) ◽  
pp. 413-421 ◽  
Author(s):  
Penghao Wu ◽  
Haochuan Li ◽  
Jiaojiao Ren ◽  
Shaojiang Chen
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate

scholarly journals Development of In Vivo Haploid Inducer Lines for Screening Haploid Immature Embryos in Maize

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 739
Author(s):  
Chen Chen ◽  
Zijian Xiao ◽  
Junwen Zhang ◽  
Wei Li ◽  
Jinlong Li ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Immature Embryos ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate ◽  
Haploid Inducer ◽  
Breeding Populations ◽  
Selection For ◽  
Doubled Haploid Breeding

Doubled haploid technology is widely applied in maize. The haploid inducer lines play critical roles in doubled haploid breeding. We report the development of specialized haploid inducer lines that enhance the purple pigmentation of crossing immature embryos. During the development of haploid inducer lines, two breeding populations derived from the CAU3/S23 and CAU5/S23 were used. Molecular marker-assisted selection for both qhir1 and qhir8 was used from BC1F1 to BC1F4. Evaluation of the candidate individuals in each generation was carried out by pollinating to the tester of ZD958. Individuals with fast and clear pigmentation of the crossing immature embryos, high number of haploids per ear, and high haploid induction rate were considered as candidates. Finally, three new haploid inducer lines (CS1, CS2, and CS3) were developed. The first two (CS1 and CS2) were from the CAU3/S23, with a haploid induction rate of 8.29%–13.25% and 11.54%–15.54%, respectively. Meanwhile, the CS3 was from the CAU5/S23. Its haploid induction rate was 8.14%–12.28%. In comparison with the donor haploid inducer lines, the 24-h purple embryo rates of the newly developed haploid inducer lines were improved by 10%–20%, with a ~90% accuracy for the identification of haploid immature embryos. These new haploid inducer lines will further improve the efficiency of doubled haploid breeding of maize.

scholarly journals Mapping of QTL for kernel abortion caused by in vivo haploid induction in maize (Zea mays L.)

PLoS ONE ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. e0228411 ◽  
Author(s):  
Yanzhi Qu ◽  
Penghao Wu ◽  
Jiaojiao Ren ◽  
Zonghua Liu ◽  
Jihua Tang ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Haploid Induction

Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize

Euphytica ◽  
2011 ◽  
Vol 180 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Aida Z. Kebede ◽  
Baldev S. Dhillon ◽  
Wolfgang Schipprack ◽  
Jose L. Araus ◽  
Marianne Bänziger ◽  
...  
Keyword(s):  
Tropical Maize ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate

Utilization of Cogon (Imperata cylindrica L.) Silage and Urea-Treated Corn (Zea mays L.) Stover as Affected by Supplementation

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Elmar Patiga ◽  
◽  
Lolito Bestil ◽  
Hershey Mondejar ◽  
◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Imperata Cylindrica

In vivo, urea-treated, silage, concentrate: Ipil-ipil supplement

In Vivo Evaluation of a Brown Midrib Mutant of Zea Mays L.

1972 ◽  
Vol 35 (4) ◽  
pp. 883-889 ◽  
Author(s):  
L. D. Muller ◽  
V. L. Lechtenberg ◽  
L. F. Bauman ◽  
R. F. Barnes ◽  
C. L. Rhykerd
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Brown Midrib ◽  
In Vivo Evaluation

scholarly journals Baked corn (Zea mays L.) and cooked common bean (Phaseolus vulgaris L.) chips improved enzymatic biomarkers and alleviated inflammation during chronic colitis in vivo (P06-063-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Ivan Luzardo ◽  
Rocio Campos-Vega ◽  
Elvira Gonzalez de Mejia ◽  
Flavia Loarca
Keyword(s):  
Zea Mays ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Zea Mays L ◽  
Relative Weight ◽  
Inflammatory Cells ◽  
Phaseolus Vulgaris L ◽  
Chronic Colitis ◽  
Gut Barrier Function

Abstract Objectives The aim of this research was to evaluate the anti-inflammatory properties of an oven-baked nixtamalized corn (Zea mays L.)/cooked common bean (Phaseolus vulgaris L.) chip in a model of chronic colitis in vivo. The hypothesis was that the chip consumption would prevent colon barrier disruption and improve enzymatic biomarkers by the modulation of infiltration and adhesion of inflammatory cells. Methods A 70% corn and 30% bean chip (7030C) was evaluated, using dextran sodium sulfate (DSS, 2% v/v) as chemical inductor of colitis. After 1-week of acclimatization, 36 CD-1 male mice (6–8 weeks age) were randomly divided into 5 groups: G1 (negative control, fed with basal diet, BD, and water); G2 (positive control, BD + DSS), G3 (100 g 7030C/kg body weight, BW/day + BD), G4 (200 g 7030C/kg BW + BD) and G5 (300 g 7030C/kg BW + BS). The G2-G5 groups were administered DSS every other week, during 5-weeks.After the mice were euthanized, BW and disease activity index (DAI) were recorded. Liver, colon, and spleen were collected, weighed and analyzed for histology. Colonic myeloperoxidase (MPO)/fecal b-glucuronidase (GLUC) activities were also quantified, as well as fecal/cecal metabolites. The colonic mRNA expression of inflammation-associated genes was conducted using a gene inflammation profiler array. Results DSS increased DAI up to 2 units, BW loss was 10–17%, and induced colon shortening 10–15%. Compared to G2, G4 exhibited significantly (p < 0.05) lower DAI (0.75 ± 0.01), spleen relative weight (0.003 ± 0.0001) and colon weight/length ratio (0.045 ± 0.008). The histological analysis showed that the chip consumption prevented colonic barrier damage. G4 displayed the lowest MPO and GLUC among all DSS-induced groups (0.004 ± 0.0004 mU/mg colon; 0.44 ± 0.01 mmol/min/g feces, respectively), and the lowest seric content of MCP-1 protein. Amid all the quantified metabolites, the chip consumption significantly reduced the fecal/cecal content of acetic acid, while butyric and propionic increased at the end of the study. Inflammation gene expression was modulated by the chip consumption. Conclusions Our results suggest that the consumption of this chip might alleviate chronic colitis symptoms because of a protective effect in the gut barrier function and the modulation of infiltration of inflammatory cells. Funding Sources The funding received by CONACyT, CONCyTEQ-Mexico and NIFA-USDA-HATCH are appreciated.

scholarly journals MYO, a Candidate Gene for Haploid Induction in Maize Causes Male Sterility

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 773
Author(s):  
Kimberly Vanous ◽  
Thomas Lübberstedt ◽  
Rania Ibrahim ◽  
Ursula K. Frei
Keyword(s):  
Male Sterility ◽  
Gene Isolation ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Breeding Programs ◽  
Positional Candidate ◽  
Haploid Induction Rate ◽  
Induction Rate ◽  
Transgenic Lines ◽  
Strong Candidate

Doubled haploid technology is highly successful in maize breeding programs and is contingent on the ability of maize inducers to efficiently produce haploids. Knowledge of the genes involved in haploid induction is important for not only developing better maize inducers, but also to create inducers in other crops. The main quantitative trait loci involved in maize haploid induction are qhir1 and qhir8. The gene underlying qhir1 has been discovered and validated by independent research groups. Prior to initiation of this study, the gene associated with qhir8 had yet to be recognized. Therefore, this research focused on characterizing positional candidate genes underlying qhir8. Pursuing this goal, a strong candidate for qhir8, GRMZM2G435294 (MYO), was silenced by RNAi. Analysis of crosses with these heterozygous RNAi-transgenic lines for haploid induction rate revealed that the silencing of MYO significantly enhanced haploid induction rate by an average of 0.6% in the presence of qhir1. Recently, GRMZM2G465053 (ZmDMP) was identified by map-based gene isolation and shown to be responsible for qhir8. While our results suggest that MYO may contribute to haploid induction rate, results were inconsistent and only showing minor increases in haploid induction rate compared to ZmDMP. Instead, reciprocal crosses clearly revealed that the silencing of MYO causes male sterility.

scholarly journals Development of a New Maize (Zea mays L.) Breeding Program

2002 ◽  
pp. 25-30
Author(s):  
Pál Pepó ◽  
Szilárd Tóth
Keyword(s):  
Zea Mays ◽  
Recurrent Selection ◽  
Zea Mays L ◽  
Genetic Manipulation ◽  
Dominant Gene ◽  
Breeding Program ◽  
Regeneration Ability ◽  
Breeding Programs

Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.

scholarly journals Evaluation of the in vitro and in vivo antihypertensive effect and antioxidant activity of blue corn hydrolysates derived from wet-milling//Evaluación del efecto antihipertensivo in vitro e in vivo y actividad antioxidante del hidrolizado de maíz azul derivado de la molienda húmeda

Biotecnia ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Alvaro Montoya-Rodríguez ◽  
Evelyn Isabel Osuna-Gallardo ◽  
Francisco Cabrera-Chávez ◽  
Jorge Milán-Carrillo ◽  
Cuauhtémoc Reyes-Moreno ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Antioxidant Activity ◽  
Zea Mays ◽  
Antihypertensive Effect ◽  
Zea Mays L ◽  
Enzymatic Digestion ◽  
Wet Milling ◽  
Food Ingredient

Hypertension is considered a risk factor for coronary heart disease, and its prevalence has increased substantially. Inhibition of angiotensin-converting enzyme (ACE-I) is key to lower blood pressure, making it an excellent treatment for hypertension. Corn (Zea mays L.) is an important source of bioactive peptides with potential anti-hypertensive activity related to ACE-I inhibition. These peptides can be obtained through the hydrolysis of corn gluten meal (CGM), as wetmilling by-products. The aim was to evaluate the in vitro and in vivo ACE-I inhibitory activity of blue CGM hydrolysates. Enzymatic digestion in vitro of blue CGM was conducted at different times. Hydrolysis for 360 min significantly increased both soluble protein and antioxidant activity by 4 and 8-fold respectively, the maximum ACE-I inhibition (94.3 %) was observed with 260 min hydrolysate. Mice were treated with the blue CGM hydrolysate (260 min), captopril or PBS to test the bioavailability in vivo. The CGM hydrolysate was detected in serum after 5 and up to 30 min after ingestion, showing the maximum ACE-I inhibitory capacity (59 %) during the first 15 min. Overall, this work showed that the blue CGM hydrolysate could serve as a functional food ingredient with antihypertensive effect due to its blood pressure-lowering peptides.RESUMENLa hipertensión es factor de riesgo en enfermedades coronarias, y su prevalencia ha aumentado sustancialmente. La inhibición de enzima convertidora de angiotensina (ECA) es clave para disminuir presión arterial, y excelente tratamiento para hipertensión. El maíz (Zea mays L.) es fuente de péptidos bioactivos con actividad antihipertensiva por inhibición de ECA. Péptidos pueden obtenerse por hidrólisis de harina de gluten de maíz (HGM), como subproducto de molienda húmeda. El objetivo fue evaluar in vitro e in vivo actividad inhibitoria de ECA en hidrolizados de HGM azul. La digestión enzimática in vitro de HGM fue conducida a diferentes tiempos. La hidrólisis por 360 min aumento significativamente proteína soluble y actividad antioxidante de 4 y 8 veces, respectivamente; la máxima inhibición de ECA (94.3 %) fue observada a 260 minutos del hidrolizado. Ratones fueron tratados con HGM hidrolizado (260 minutos), captopril o PBS para evaluar biodisponibilidad in vivo. Después de la ingestión, HGM hidrolizado fue detectado en suero en 5 hasta 30 minutos, mostrando máxima inhibición de ECA (59 %) durante los primeros 15 minutos. En general, este trabajo mostró que hidrolizado de HGM podría servir como ingrediente funcional en alimentos con efecto antihipertensivo debido a péptidos reductores de presión arterial.

Sign in / Sign up

Export Citation Format

Share Document