Micro-scale chlorophyll analysis and developmental expression of a cytokinin oxidase/dehydrogenase gene during leaf development and senescence

2011 ◽  
Vol 66 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Thomas Evans ◽  
Jiancheng Song ◽  
Paula E. Jameson
Keyword(s):  
Leaf Development ◽  
Developmental Expression ◽  
Cytokinin Oxidase ◽  
Micro Scale ◽  
Dehydrogenase Gene ◽  
Chlorophyll Analysis

Using an Inverse PCR Method to Clone the Wheat Cytokinin Oxidase/Dehydrogenase Gene TaCKX1

2008 ◽  
Vol 26 (3) ◽  
pp. 143-155 ◽  
Author(s):  
De-Shun Feng ◽  
Hong-Gang Wang ◽  
Xian-Sheng Zhang ◽  
Ling-Rang Kong ◽  
Ji-Chun Tian ◽  
...  
Keyword(s):  
Inverse Pcr ◽  
Cytokinin Oxidase ◽  
Dehydrogenase Gene ◽  
Pcr Method

scholarly journals Enhanced drought and heat stress tolerance of tobacco plants with ectopically enhanced cytokinin oxidase/dehydrogenase gene expression

2013 ◽  
Vol 64 (10) ◽  
pp. 2805-2815 ◽  
Author(s):  
Hana Macková ◽  
Marie Hronková ◽  
Jana Dobrá ◽  
Veronika Turečková ◽  
Ondřej Novák ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Stress Tolerance ◽  
Cytokinin Oxidase ◽  
Tobacco Plants ◽  
Dehydrogenase Gene ◽  
Heat Stress Tolerance ◽  
Drought And Heat Stress

scholarly journals A VIN3-like Protein OsVIL1 Is Involved in Grain Yield and Biomass in Rice

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 83
Author(s):  
Jinmi Yoon ◽  
Hee-Joong Jeong ◽  
Gibeom Baek ◽  
Jungil Yang ◽  
Xin Peng ◽  
...  
Keyword(s):  
Grain Yield ◽  
Cross Sections ◽  
Protein Complexes ◽  
Floral Organ ◽  
Cytokinin Oxidase ◽  
Basal Region ◽  
Post Translational Modification ◽  
Polycomb Repressive Complex 2 ◽  
Dehydrogenase Gene ◽  
Multimeric Protein

In chromatin remodeling, the post-translational modification of histone proteins is mediated by multimeric protein complexes. VERNALIZATION INSENSITIVE3 (VIN3) forms a complex with Polycomb Repressive Complex 2 (PRC2), which mediates the trimethylation of H3K27 to repress target gene expression. In rice, four genes (OsVIL1-OsVIL4) encoding the VIN3-like proteins are expressed ubiquitously in various tissues. Null mutants of osvil2 display pleiotropic phenotypes such as altered flowering time, floral organ defects, and reduced tiller size. In contrast, osvil1 mutants did not show significant phenotypes except in fertilization compared with the wild type. However, transgenic plants overexpressing OsVIL1 showed phenotypes of increased biomass and grain yield. Cross-sections of the basal region of elongating stems revealed that the increased biomass was mediated by inducing cell proliferation in the meristem. Chromatin immunoprecipitation assay indicated that OsVIL1 repressed expression of cytokinin oxidase/dehydrogenase gene (OsCKX2) by binding to the promoter and genic regions of OsCKX2. We also observed that OsVIL1 modified the levels of H3K27me3 in the OsCKX2 chromatin. Because OsCKX2 encodes an enzyme that degrades active cytokinin, we conclude that OsVIL1 functions in the regulation of endogenous active cytokinin levels, thereby increasing plant height and productivity.

scholarly journals Identification and quantitative expression of cytokinin regulatory genes during seed and leaf development in wheat

2010 ◽  
Vol 14 ◽  
pp. 131-137
Author(s):  
J. Song ◽  
L. Jiang ◽  
P.E. Jameson
Keyword(s):  
Leaf Development ◽  
Flag Leaf ◽  
Expression Patterns ◽  
Mrna Level ◽  
Gene Families ◽  
Cytokinin Oxidase ◽  
Isopentenyl Transferase ◽  
Quantitative Expression ◽  
Qrt Pcr ◽  
Yield Determination

Cytokinins are intimately involved in plant growth and development and their concentration is known to change dramatically during early stages of seed development. We propose that the concentration of active cytokinin may be coordinately regulated by specific member(s) of the multi-gene families encoding biosynthesis (isopentenyl transferase, IPT), catabolism (cytokinin oxidase, CKX), and metabolism (zeatin glucosyltransferases (ZOG) and β-glucosidase (GLU)) genes. Our qRT-PCR data for 22 putative genes showed that the expression patterns of individual members of the Triticum aestivum (Ta)IPTs, TaCKXs, TaZOGs, and TaGLUs multi-gene families were tissue and development specific during seed and flag leaf development, with up to 90-fold changes in mRNA level. Key genes that may be involved in seed yield determination have been identified. Keywords: Wheat, flag leaf, cytokinin genes, quantitative expression, qRT-PCR

Cloning and Expression Analysis of Wheat Cytokinin Oxidase/Dehydrogenase Gene TaCKX3

2010 ◽  
Vol 29 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Xin Ma ◽  
De-Shun Feng ◽  
Hong-Gang Wang ◽  
Xing-Feng Li ◽  
Ling-Rang Kong
Keyword(s):  
Expression Analysis ◽  
Cloning And Expression ◽  
Cytokinin Oxidase ◽  
Dehydrogenase Gene

scholarly journals Canopy shade causes a rapid and transient arrest in leaf development through auxin-induced cytokinin oxidase activity

2007 ◽  
Vol 21 (15) ◽  
pp. 1863-1868 ◽  
Author(s):  
M. Carabelli ◽  
M. Possenti ◽  
G. Sessa ◽  
A. Ciolfi ◽  
M. Sassi ◽  
...  
Keyword(s):  
Leaf Development ◽  
Oxidase Activity ◽  
Cytokinin Oxidase
Sign in / Sign up

Export Citation Format

Share Document