Cytokinin receptor CRE1 is required for the defense response of Nicotiana tabacum to Chilli veinal mottle virus

2020 ◽  
Vol 90 (3) ◽  
pp. 545-555 ◽  
Author(s):  
Wenshan Zou ◽  
Lijuan Chen ◽  
Jialing Zou ◽  
Hongyan Han ◽  
Chunyan Fei ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Defense Response ◽  
Mottle Virus ◽  
Cytokinin Receptor ◽  
Chilli Veinal Mottle Virus

The role of phytochromes in Nicotiana tabacum against Chilli veinal mottle virus

2019 ◽  
Vol 139 ◽  
pp. 470-477 ◽  
Author(s):  
Chunyan Fei ◽  
Lijuan Chen ◽  
Ting Yang ◽  
Wenshan Zou ◽  
Honghui Lin ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Mottle Virus ◽  
Chilli Veinal Mottle Virus

scholarly journals Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

2020 ◽  
Vol 71 (18) ◽  
pp. 5656-5668
Author(s):  
Ting Yang ◽  
Long Qiu ◽  
Wanying Huang ◽  
Qianyi Xu ◽  
Jialing Zou ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Virus Infection ◽  
Rna Silencing ◽  
Mottle Virus ◽  
Systemic Necrosis ◽  
C Terminus ◽  
Silencing Suppression ◽  
Helper Component Proteinase ◽  
Chilli Veinal Mottle Virus

Abstract Plant symptoms are derived from specific interactions between virus and host components. However, little is known about viral or host factors that participate in the establishment of systemic necrosis. Here, we showed that helper component proteinase (HCPro), encoded by Chilli veinal mottle virus (ChiVMV), could directly interact with catalase 1 (CAT1) and catalase 3 (CAT3) in the cytoplasm of tobacco (Nicotiana tabacum) plants to facilitate viral infection. In vitro, the activities of CAT1 and CAT3 were inhibited by the interaction between HCPro and CATs. The C-terminus of HCPro was essential for their interaction and was also required for the decrease of enzyme activities. Interestingly, the mRNA and protein level of CATs were up-regulated in tobacco plants in response to ChiVMV infection. Nicotiana tabacum plants with HCPro overexpression or CAT1 knockout were more susceptible to ChiVMV infection, which was similar to the case of H2O2-pre-treated plants, and the overexpression of CAT1 inhibited ChiVMV accumulation. Also, neither CAT1 nor CAT3 could affect the RNA silencing suppression (RSS) activity of HCPro. Our results showed that the interaction between HCPro and CATs promoted the development of plant systemic necrosis, revealing a novel role for HCPro in virus infection and pathogenicity.

scholarly journals Occurrence of Chilli veinal mottle virus in Nicotiana tabacum in Yunnan, China

Plant Disease ◽  
2011 ◽  
Vol 95 (3) ◽  
pp. 357-357 ◽  
Author(s):  
M. Ding ◽  
C. Yang ◽  
L. Zhang ◽  
Z.-L. Jiang ◽  
Q. Fang ◽  
...  
Keyword(s):  
Particle Size ◽  
Nicotiana Tabacum ◽  
Yunnan Province ◽  
Sandwich Elisa ◽  
Mottle Virus ◽  
Economic Losses ◽  
Rt Pcr ◽  
Tobacco Plants ◽  
Thin Sections ◽  
Chilli Veinal Mottle Virus

Flue-cured tobacco (Nicotiana tabacum) is an important crop in Yunnan Province, China. During a survey in July 2010, tobacco plants (N. tabacum cv. Yunyan 85) in the fields near Dali County in the northwest Yunnan Province of China had symptoms of chlorosis along leaf veins and later showed symptoms of white or brown necrosis along the veins. In 10 surveyed fields in Baizhishu Village in the city of Weishan, a commercial tobacco field (10 ha) developed virus-like disease symptoms; the incidence of affected plants ranged from 0.5 to 3%, which caused obvious economic losses. An isolate (YN75) was collected at random from five symptomatic leaves sampled from five plants. Negative staining of crude extracts of the infected leaves and subsequent electron microscopy revealed flexuous rods of 12 to 13 × 750 nm. Pinwheel-like inclusion bodies were abundant in thin sections of infected leaves. The particle size suggested a species of Potyviridae. Thus, the isolate was assayed in double antibody sandwich-ELISA (Agdia, Elkhart, IN) for the presence of Potato virus Y, Tobacco etch virus, and Tobacco vein mottling virus. All antigens gave negative results. Total RNA was extracted from leaves and tested by reverse transcription (RT)-PCR. The primer M4-T (5′-GTT TTC CCA GTC ACG ACT TTT TTT TTT TTT TT-3′) was employed for cDNA synthesis described by Chen et al (1). The primer set ChiVMV-F (5′-TAG TTG YGC ATA C (C/G) C AGG AGA GAG-3′)/M4 (5′-GTT TTC CCA GTC ACG AC-3′) is complimentary to the region of coat protein and 3′-untranslated region of Chilli veinal mottle virus (ChiVMV), respectively. The expected 1,189-bp fragments were amplified from RNA templates and the amplicon was cloned and sequenced (GenBank Accession No. HQ218936). Comparisons of amplicons with the amino acid sequence available in the NCBI database using BLAST showed 91.4% identity with ChiVMV from India (GenBank Accession No. EF213688) and 90.7% with ChiVMV from Taiwan (Accession No. DQ854950). The virus particle size, RT-PCR results, and sequence data revealed that these tobacco plants were infected by ChiVMV. To our knowledge, this is the first report of ChiVMV infecting N. tabacum in China. Reference: (1) J. Chen et al. Arch. Virol. 146:757, 2001.

scholarly journals First report of Chilli veinal mottle virus in Naga chilli (Capsicum chinense) in Meghalaya, India

VirusDisease ◽  
2013 ◽  
Vol 25 (1) ◽  
pp. 142-143 ◽  
Author(s):  
Amrita Banerjee ◽  
Ram Dutta ◽  
Somnath Roy ◽  
S. V. Ngachan
Keyword(s):  
Mottle Virus ◽  
Capsicum Chinense ◽  
First Report ◽  
Chilli Veinal Mottle Virus

scholarly journals KETAHANAN BEBERAPA KULTIVAR CABAI TERHADAP CUCUMBER MOSAIC VIRUS DAN CHILLI VEINAL MOTTLE VIRUS

2012 ◽  
Vol 7 (2) ◽  
pp. 130-139
Author(s):  
Muhammad Taufik ◽  
Sri Hendrastuti Hidayat ◽  
Sriani Sujiprihati ◽  
Gede Suastika ◽  
Sientje Mandang Sumaraw
Keyword(s):  
Virus Infection ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Yield Loss ◽  
Disease Incidence ◽  
Growth And Yield ◽  
Mottle Virus ◽  
Tolerance Response ◽  
Chilli Veinal Mottle Virus ◽  
Das Elisa

Resistance Evaluation of Chillipepper Cultivars for Cucumber Mosaic Virus and Chilli Veinal Mottle Virus.  The use of resistance culivars is an important strategy for management of virus infection in chillipepper. A research was undergone to study the effect of single and mix infection of CMV and ChiVMV on the disease incidence and on the growth and yield of nine chillipepper cultivars, i.e. Cilibangi 4, Cilibangi 5, Cilibangi 6, Helem, Jatilaba, Tit Bulat, Tit Segitiga, Tit Super and Tampar. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA.  In general, inoculated chillipepper cultivars developed similar symptoms, i.e. mosaic type for CMV and mottle type for ChiVMV.  More severe symptom was not always observed from mix infection of CMV and ChiVMV. Disease incidence occurred in the range of 16.67 – 86.0% and this caused 18.3 – 98.6% yield loss.  Based on symptom expression, ELISA result, and reduction on yield, it can be concluded that all chillipepper cultivars used in this study could not hold up the virus infection. However, several cultivars showed tolerance response :  Jatilaba, Tit Super, and Tampar for CMV; Cilibangi 4 for ChiVMV; Tit Super for mix infection; and Cilibangi 5 for CMV, ChiVMV, and mix infection.  Further evaluation and investigation involving different chillipepper cultivars should be conducted.

N gene enhances resistance to Chilli veinal mottle virus and hypersensitivity to salt stress in tobacco

2018 ◽  
Vol 230 ◽  
pp. 92-100 ◽  
Author(s):  
Ting Yang ◽  
Zhen-peng Xu ◽  
Rui Lv ◽  
Li-sha Zhu ◽  
Qi-ding Peng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Mottle Virus ◽  
N Gene ◽  
Chilli Veinal Mottle Virus

scholarly journals METODE PENAPISAN CABAI (CAPSICUM ANNUUM L.) UNTUK KETAHANAN TERHADAP CHILLI VEINAL MOTTLE VIRUS (Chi VMV) DAN CUCUMBER MOSAIC VIRUS (CMV)

2011 ◽  
Vol 8 (2) ◽  
pp. 146-153
Author(s):  
Latifah Latifah ◽  
Sri Hendrastuti Hidayat ◽  
Sriani Sujiprihati
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Protection ◽  
Disease Incidence ◽  
Severe Disease ◽  
Screening Method ◽  
Mottle Virus ◽  
Inoculation Methods ◽  
Virus Sequence ◽  
Chilli Veinal Mottle Virus

Screening Method for Chilli Veinal Mottle Virus  (Chi VMV) and Cucumber Mosaic Virus  (CMV) Resistance in Chillipepper.  ChiVMV and CMV have been reported as the causal agents of main diseases in chillipepper in Indonesia and other Asian countries.  Mix infection of this two viruses was commonly occurred in the field, causing severe disease .  The use of resistance varieties has been proposed for dealing with the yield losses causing by  the viruses.  Breeding program is undergoing for development of chillipepper varieties resistant to ChiVMV and CMV.  Methodology for routine screening activity of chillipepper for resistance to both ChiVMV and CMV needs to be established. This research was conducted in Cikabayan Glass House and Plant Virology Laboratory, Plant Protection Department, Bogor Agricultural University from May 2006 to June 2007. Aim of the research was to develop screening method for simultaneous infection by the two viruses, ChiVMV and CMV.  Inoculation of ChiVMV and CMV was done by single inoculation or repetitive inoculation methods.  In both methods, ChiVMV and CMV were inoculated in different sequences, either ChiVMV or CMV first.  The result showed that incubation period was shorter when CMV was inoculated in advance both in single and repetitive inoculation method.  Mosaic, mottle and malformation type symptom was observed in infected plants. Based on disease incidence, infection of ChiVMV was higher compared to CMV in repetitive inoculation as well as in single inoculation.  Repetitive inoculation methods with virus sequence ChiVMV-CMV-ChiVMV-CMV  was selected for resistance evaluation of chillipepper genotypes.

scholarly journals Natural Occurrence of Chilli veinal mottle virus on Capsicum chinense in China

Plant Disease ◽  
2006 ◽  
Vol 90 (3) ◽  
pp. 377-377 ◽  
Author(s):  
J. Wang ◽  
Z. Liu ◽  
S. Niu ◽  
M. Peng ◽  
D. Wang ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Viral Disease ◽  
Mottle Virus ◽  
Major Protein ◽  
Natural Occurrence ◽  
Capsicum Chinense ◽  
Thin Sections ◽  
First Report ◽  
Chilli Veinal Mottle Virus

An outbreak of a viral disease on chili pepper (Capsicum chinense Jacp. cv. Yellow Lantern) occurred in Hainan Province, China during 2003 and 2004. The disease was prevalent in five chili-producing counties surveyed. Leaves of infected plants initially displayed symptoms of dark green banding along veins and later became distorted with striking mosaic. Infected plants had reduced flower numbers and fruit set, resulting in a significant yield loss. The causative virus was characterized and identified as Chilli veinal mottle virus (ChiVMV) (3). An isolate of the virus was obtained via three single lesion passages through Chenopodium amaranticolor and was shown to reproduce the same symptoms on inoculated C. chinense cv. Yellow Lantern. Negative staining of crude extracts of the infected tissue and subsequent electron microscopy revealed flexuous rods of 12 to 13 × 750 nm, typical of a potyvirus. Pinwheel-like inclusion bodies were abundant in thin sections of infected leaves. Purified virus preparations contained one major protein of 32.8 kDa and one minor protein of 28 kDa when fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both of these protein bands were excised and subsequently analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Multiple peptide fragments from both proteins were identified as arising from ChiVMV capsid protein (CP) (1,2). Therefore, the 32.8-kDa protein is the full-length ChiVMV CP and the 28-kDa protein is presumably a degradation product of the CP. The combined biological and molecular data provided strong evidence that the viral disease on C. chinense was caused by ChiVMV. To our knowledge, this is the first report of ChiVMV infection on C. chinense in China and the first report of C. amaranticolor as an experimental host for ChiVMV. References: (1) P. Chiemsombat et al. Arch. Virol. 143:1855, 1998. (2). J. Joseph and H. S. Savithri. Arch. Virol. 144:1679, 1999. (3) P. Siriwong et al. Plant Pathol. 44:718, 1995.

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