Background:
FSH Receptor Binding Inhibitor (FRBI) blocked the binding of FSH to FSHR. Our
initial study revealed FRBI reduced the maturation rate, enhanced the apoptosis of sheep Cumulus-Oocyte
Complex (COCs). Little is known about whether FRBI modulates ERβ and FSHR levels in the normal uterine
and cancerous tissues. The present study aimed to evaluate the FRBI effects on the expressions of Estrogen
Receptor-beta (ERβ) and FSH receptor (FSHR) in the uteri.
Methods:
Methods: 150 mice were assigned to FRBI+FSH (COM), FSH and control groups (CG). Mice of COM-1,
COM-2 and COM-3 groups were simultaneously intramuscularly injected with 500, 750 and 1000 µg FRBI with
10 IU FSH, respectively for five days. Western blotting and qPCR were utilized to determine the expression of
ERβ and FSHR.
Results:
In comparison with FSH group, uterine lumen and glands of COM groups became narrow. The uterine
wall and endometrial epithelium were thinned, and uterine lumen became narrow. Epithelial cells were decreased.
Uterine wall thicknesses of COM-1, COM-2 and COM-3 groups were reduced by 6.49%, 14.89% and
15.69% on day 30 as compared with FSH group. Uterine perimetrium thicknesses of COM-1, COM-2 and
COM-3 groups were reduced by 16.17%, 17.93% and 19.92% on day 20 in comparison with FSH group. Levels
of FSHR mRNAs and proteins of COM-1, COM-2 and COM-3 groups were less than FSH group on days 20
and 30 (P<0.05). ERβ protein of COM-3 group was less than FSH group. Serum estradiol (E2) and FSH concentrations
of COM-2 and COM-3 were lower than that of FSH group on day 30.
Conclusion:
FRBI could decrease UWT and UPT, also block the uterine development, decline expression levels
of ERβ and FSHR protein. Additionally, FRBI reduced the secretion of secretion of FSH and E2. Downregulating
expression of FSHR and ERβ may be a potential treatment regimen for cervical cancer patients.
The carriers of the A/G-G/G allelic combination of the c.2039 A>G and c.-29 G>A FSH receptor polymorphisms retrieve the highest number of oocytes in IVF/ICSI cycles
