scholarly journals Increase of leucocyte-derived extracellular traps (ETs) in semen samples from human acute epididymitis patients—a pilot study

2020 ◽  
Vol 37 (9) ◽  
pp. 2223-2231
Author(s):  
Fabiola Zambrano ◽  
Mabel Schulz ◽  
Adrian Pilatz ◽  
Florian Wagenlehner ◽  
Hans-Christian Schuppe ◽  
...  
Keyword(s):  
Pilot Study ◽  
Extracellular Traps ◽  
Acute Epididymitis

scholarly journals Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study

2020 ◽  
Vol 27 (1) ◽  
pp. 107327482096047
Author(s):  
Marzena Garley ◽  
Ewa Jabłońska ◽  
Wojciech Miltyk ◽  
Kamil Grubczak ◽  
Arkadiusz Surażyński ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Squamous Cell Carcinoma ◽  
Pilot Study ◽  
Healthy Subjects ◽  
Neutrophil Extracellular Traps ◽  
Indirect Contact ◽  
Phosphorylated Proteins ◽  
Extracellular Traps ◽  
Western Blot Method ◽  
And Control

The aim of the experiment was to evaluate the process of neutrophil extracellular traps (NETs) formation in patients with oral squamous cell carcinoma (OSCC) in response to direct or indirect contact with SCC cells in comparison to results obtained in the cells of healthy subjects. To fulfill study objectives CAL 27 cell line and blood were obtained from cancer patients and control subjects. Parameters related to NETs formation were analyzed utilizing flow cytometry, fluorescence microscopy, and ELISA-type tests. The expression of selected phosphorylated proteins of the PI3K/Akt/PBK pathway in neutrophils was evaluated using the Western blot method. An increase in NETs formation was observed in a coculture of neutrophils with SCC cells, with the largest amount of NETs formed after stimulation with a supernatant obtained from the SCC culture. The enhanced process of NETs formation was accompanied by changes in the expression of proteins from the PI3K/Akt/PBK pathway. The obtained results prove the existence of interactions between neutrophils and cancer cells resulting in NETosis with the participation of the PI3K/Akt/PBK pathway in patients with OSCC.

The impact of physical training on neutrophil extracellular traps in young male athletes – a pilot study

2021 ◽  
Author(s):  
Joanna Orysiak ◽  
Jitendra K. Tripathi ◽  
Klaudia K. Brodaczewska ◽  
Atul Sharma ◽  
Konrad Witek ◽  
...  
Keyword(s):  
Pilot Study ◽  
Physical Training ◽  
Young Male ◽  
Neutrophil Extracellular Traps ◽  
Male Athletes ◽  
Extracellular Traps ◽  
The Impact

Characterization and comparison of neutrophil extracellular traps in gingival samples of periodontitis and gingivitis: A pilot study

2018 ◽  
Vol 54 (3) ◽  
pp. 218-224 ◽  
Author(s):  
Antonio Magán‐Fernández ◽  
Francisco O'Valle ◽  
Francisco Abadía‐Molina ◽  
Ricardo Muñoz ◽  
Patricia Puga‐Guil ◽  
...  
Keyword(s):  
Pilot Study ◽  
Neutrophil Extracellular Traps ◽  
Extracellular Traps

scholarly journals Markers of neutrophil activation and extracellular traps formation are predictive of appendicitis in mice and humans: a pilot study

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Michael Boettcher ◽  
Melina Esser ◽  
Julian Trah ◽  
Stefan Klohs ◽  
Nariman Mokhaberi ◽  
...  
Keyword(s):  
Pilot Study ◽  
Disease Severity ◽  
Bacterial Infections ◽  
Tissue Expression ◽  
Neutrophil Activation ◽  
Immune Defense ◽  
Predictive Values ◽  
Extracellular Traps ◽  
Reflect Disease Severity ◽  
Inflammation Reaction

Abstract Appendicitis is one of the most frequent emergencies in pediatric surgery, yet current biomarkers for diagnosis are unspecific and have low predictive values. As neutrophils and extracellular traps (ETs) are an essential component of the immune defense against bacterial infections, and appendicitis is considered an inflammation reaction of the appendix, we hypothesized that neutrophil activation and NET formation play an essential role in appendicitis development and maintenance. Therefore, this pilot study aimed to establish a murine model of appendicitis and to evaluate ETs markers to diagnose appendicitis in mice and humans. The study used 20 (12 appendicitis- and 8 controls) 6-week old mice which underwent advanced appendicitis induction using a modified caecal ligation puncture procedure. During the study, cell-free DNA, neutrophil elastase (NE), myeloperoxidase (MPO), and citrullinated Histone H3 (H3cit) were assessed. Additionally, samples of 5 children with histologically confirmed appendicitis and 5 matched controls with catarrhal appendicitis, were examined for the same biomarkers. Moreover, NE, MPO, and H3cit were assessed histologically via immunofluorescence in mice and humans. All mice in the appendicitis group developed an advanced form of appendicitis with focal peritonitis. In mice and humans with appendicitis, markers of neutrophil activation and ETs formation (especially cfDNA, NE and H3cit) were significantly elevated in blood and tissue compared to controls. Ultimately, biomarkers correlated extremely well with tissue expression and thus disease severity. It appears that neutrophil activation and possibly NETs contribute to appendicitis development and biomarkers of neutrophil activation and ET formation reflect disease severity and thus could be used as biomarkers for appendicitis. However, large prospective clinical studies are needed to confirm our findings.

Determination of leucocyte extracellular traps (ETs) in seminal fluid (ex vivo ) in infertile patients—A pilot study

Andrologia ◽  
2019 ◽  
Vol 51 (9) ◽  
Author(s):  
Mabel Schulz ◽  
Fabiola Zambrano ◽  
Hans‐Christian Schuppe ◽  
Florian Wagenlehner ◽  
Anja Taubert ◽  
...  
Keyword(s):  
Pilot Study ◽  
Seminal Fluid ◽  
Ex Vivo ◽  
Extracellular Traps ◽  
Infertile Patients

Pilot study on criteria in cavity preparation-facts or artifacts?

1973 ◽  
Vol 37 (11) ◽  
pp. 27-31 ◽  
Author(s):  
G Salvendy ◽  
WM Hinton ◽  
GW Ferguson ◽  
PR Cunningham
Keyword(s):  
Pilot Study ◽  
Cavity Preparation

A Pilot Study of Early Storybook Reading With Babies With Hearing Loss

2019 ◽  
Vol 62 (9) ◽  
pp. 3397-3412
Author(s):  
Michelle I. Brown ◽  
David Trembath ◽  
Marleen F. Westerveld ◽  
Gail T. Gillon
Keyword(s):  
Hearing Loss ◽  
Pilot Study ◽  
Visual Analysis ◽  
Single Case ◽  
Eye Contact ◽  
Preliminary Evidence ◽  
Storybook Reading ◽  
Book Selection ◽  
Turn Taking ◽  
Parent Child

Purpose This pilot study explored the effectiveness of an early storybook reading (ESR) intervention for parents with babies with hearing loss (HL) for improving (a) parents' book selection skills, (b) parent–child eye contact, and (c) parent–child turn-taking. Advancing research into ESR, this study examined whether the benefits from an ESR intervention reported for babies without HL were also observed in babies with HL. Method Four mother–baby dyads participated in a multiple baseline single-case experimental design across behaviors. Treatment effects for parents' book selection skills, parent–child eye contact, and parent–child turn-taking were examined using visual analysis and Tau-U analysis. Results Statistically significant increases, with large to very large effect sizes, were observed for all 4 participants for parent–child eye contact and parent–child turn-taking. Limited improvements with ceiling effects were observed for parents' book selection skills. Conclusion The findings provide preliminary evidence for the effectiveness of an ESR intervention for babies with HL for promoting parent–child interactions through eye contact and turn-taking.

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