scholarly journals Effects of controlled ovarian stimulation on vascular barrier and endothelial glycocalyx: a pilot study

2021 ◽  
Author(s):  
Nikolai Hulde ◽  
N. Rogenhofer ◽  
F. Brettner ◽  
N. C. Eckert ◽  
I. Fetz ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Capillary Permeability ◽  
Serum Levels ◽  
Controlled Ovarian Stimulation ◽  
Endothelial Glycocalyx ◽  
Control Group ◽  
Vascular Integrity ◽  
Fluid Extravasation ◽  
Endothelial Surface ◽  
Infertility Patients

Abstract Purpose Controlled ovarian stimulation significantly amplifies the number of maturing and ovulated follicles as well as ovarian steroid production. The ovarian hyperstimulation syndrome (OHSS) increases capillary permeability and fluid extravasation. Vascular integrity intensely is regulated by an endothelial glycocalyx (EGX) and we have shown that ovulatory cycles are associated with shedding of EGX components. This study investigates if controlled ovarian stimulation impacts on the integrity of the endothelial glycocalyx as this might explain key pathomechanisms of the OHSS. Methods Serum levels of endothelial glycocalyx components of infertility patients (n=18) undergoing controlled ovarian stimulation were compared to a control group of healthy women with regular ovulatory cycles (n=17). Results Patients during luteal phases of controlled ovarian stimulation cycles as compared to normal ovulatory cycles showed significantly increased Syndecan-1 serum concentrations (12.6 ng/ml 6.1125th–19.1375th to 13.9 ng/ml 9.625th–28.975th; p=0.026), indicating shedding and degradation of the EGX. Conclusion A shedding of EGX components during ovarian stimulation has not yet been described. Our study suggests that ovarian stimulation may affect the integrity of the endothelial surface layer and increasing vascular permeability. This could explain key features of the OHSS and provide new ways of prevention of this serious condition of assisted reproduction.

Effects of controlled ovarian stimulation on vascular barrier and endothelial glycocalyx a pilot study

2020 ◽  
Author(s):  
N Rogenhofer ◽  
N Hulde ◽  
F Brettner ◽  
I Götzfried ◽  
JI Buchheim ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ovarian Stimulation ◽  
Controlled Ovarian Stimulation ◽  
Endothelial Glycocalyx

scholarly journals Endothelial Glycocalyx Layer: A Possible Therapeutic Target for Acute Lung Injury during Lung Resection

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
JiaWan Wang ◽  
AnShi Wu ◽  
Yan Wu
Keyword(s):  
Serum Albumin ◽  
Lung Resection ◽  
Serum Levels ◽  
Endothelial Glycocalyx ◽  
Major Surgery ◽  
Control Group ◽  
Vats Lobectomy ◽  
Lung Cancer Patients ◽  
Endothelial Glycocalyx Layer ◽  
And Control

Background. Shedding of the endothelial glycocalyx layer (EGL) is known to occur during major surgery, but its degradation associated with minimally invasive video-assisted thoracoscopy (VATS) remains unclear. We investigated if serum biomarkers of EGL disruption were elevated during VATS lobectomy, and whether the urinary trypsin inhibitor (UTI) ulinastatin exerted a protective effect during this procedure.Materials and Methods. Sixty ASA II-III lung cancer patients undergoing elective VATS lobectomy were divided equally into UTI and control groups. UTI group patients received intravenous UTI during surgery. Serum levels of syndecan-1 and heparan sulfate were examined before (T0) and at the end of surgery (T1). Serum albumin and hemoglobin were measured before surgery (BOD) and on the first postoperative day (POD1).Results. In control group, syndecan-1 levels were significantly elevated at T1 compared with T0 (3.77±3.15versus4.28±3.30,P=0.022⁎) and increased even more significantly in patients whose surgery lasted >3 h (3.28±2.84versus4.31±3.39,P=0.003⁎⁎). Serum albumin levels on POD1 were significantly lower in control group compared with UTI group (32.63±4.57versus35.76±2.99,P=0.031⁎).Conclusion. EGL degradation occurs following VATS lobectomy. UTI can alleviate this shedding, thus helping preserve normal vascular permeability.Trail Registration. This trial is registered withChiCTR-IOC-17010416(January 13, 2017).

scholarly journals Does female with chromosome translocation have a normal response to controlled ovarian hyperstimulation?

2020 ◽  
Vol 8 (4) ◽  
pp. 109-115
Author(s):  
Salma Kaddouri-Kaddouri ◽  
◽  
Cintia Concepción-Lorenzo ◽  
Rubí N Rodríguez-Díaz ◽  
Stephany Hess-Medler ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Male Partner ◽  
Chromosomal Translocation ◽  
Normal Karyotype ◽  
Ovarian Response ◽  
Controlled Ovarian Hyperstimulation ◽  
Chromosome Translocation ◽  
Controlled Ovarian Stimulation ◽  
Control Group ◽  
Balanced Translocation

Patients with chromosomal translocation have been reported to have high risk of reproductive failure, lower ovarian response, recurrent pregnancy loss and implantation failure. The literature is not conclusive, and our objective is to study if female balanced translocation (BT) does affect the controlled ovarian stimulation (COS). We carried out a retrospective analysis of 3249 karyotypes between 2008 and 2016, including 2276 females and 973 males. 12 women (0.5%) with BT were compared with 93 control normal karyotype group (CN) in both female and male partner. An equivalent control group (EQc) of 12 patients was additionally selected to be accurate with the BT statistical contrast. Cycle, oocyte and embryo outcomes were analysed. We concluded that female BT carriers have no diminished response to COS than infertile females with normal karyotype. This is an important information for counselling couples previous to COS and preimplantational genetic testing (PGT). Keywords: Chromosomal translocation, reciprocal, Robertsonian, controlled ovarian stimulation.

Decreased expression of aquaporin 2 is associated with impaired endometrial receptivity in controlled ovarian stimulation

2016 ◽  
Vol 28 (4) ◽  
pp. 499 ◽  
Author(s):  
Dan Zhang ◽  
Gufeng Xu ◽  
Runju Zhang ◽  
Yimin Zhu ◽  
Huijuan Gao ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Messenger Rna ◽  
Quantitative Polymerase Chain Reaction ◽  
Endometrial Receptivity ◽  
Controlled Ovarian Stimulation ◽  
Leukaemia Inhibitory Factor ◽  
In Vitro Fertilisation ◽  
Control Group ◽  
Aquaporin 2 ◽  
Embryo Attachment

Recently, there has been evidence of decreased implantation rates with in vitro fertilisation and embryo transfer due to controlled ovarian stimulation (COS). The aim of this study was to investigate the effect of COS on embryo implantation and the role of aquaporin 2 (AQP2). We recruited eight patients who underwent COS and 40 matched controls. Endometrial samples were collected on Day 4~8 after injection of human chorionic gonadotrophin in the COS group and in the mid-secretory phase in the control group. Human endometrial morphological changes after COS were examined and expression of AQP2, leukaemia inhibitory factor (LIF) and integrin B3 (ITGB3) were determined by quantitative polymerase chain reaction, western blotting and immunohistochemistry in human endometrium and Ishikawa cells. Attachment rates were obtained using the embryo attachment test. The results showed that endometrial epithelial cells from the COS group were disrupted and lacked pinopodes. Messenger RNA and protein levels of AQP2, LIF and ITGB3 decreased in endometrial samples from the COS group. Knockdown of AQP2 resulted in reduced expression of LIF and ITGB3 and reduced embryo attachment rates. In conclusion, impaired endometrial receptivity in patients who underwent COS is correlated with a decreased expression of AQP2.

Non-Alcoholic Fatty Liver Disease in Overweight Children and its Relationship with Retinol Serum Levels

2008 ◽  
Vol 78 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Suano de Souza ◽  
Silverio Amancio ◽  
Saccardo Sarni ◽  
Sacchi Pitta ◽  
Fernandes ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Liver Disease ◽  
Fatty Liver ◽  
Lipid Profile ◽  
Fatty Liver Disease ◽  
Thiobarbituric Acid ◽  
Serum Levels ◽  
Control Group ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

Objectives: To evaluate the frequency of non-alcoholic fatty liver disease, the retinol serum levels, lipid profile, and insulin resistance in overweight/obese children. To relate these biochemical variables with the risk of this disease in the population studied. Methods: The study was cross-sectional and prospective, with 46 overweight/obese school children (28 female, 18 male; mean age 8.6 years). The control group consisted of 45 children, paired by age and gender. Hepatic steatosis, evaluated by ultrasound, was classified as normal, mild, moderate, or severe. Also evaluated were serum retinol levels; thiobarbituric acid reactive substances; lipid profile; and fasting glucose and serum insulin levels, used for the calculation of the Homeostasis Model Assessment. Results: Hepatic ultrasound alterations were found in 56.5% and 48,9% of the overweight/obese and control group children, respectively. Presence of obesity was associated with high levels of triglycerides (OR = 4.6; P = 0.002). In the studied children, the risk of steatosis was related to a trend to a higher percentage of retinol inadequacy (OR = 2.8; p = 0.051); there was no association with thiobarbituric acid reactive substances, lipid profile, or insulin resistance. Conclusions: The high frequency of non-alcoholic fatty liver disease in both groups, evaluated by hepatic ultrasound, in low-socioeconomic level children, independent of nutritional condition and without significant association with insulin resistance, emphasizes that especially in developing countries, other risk factors such as micronutrient deficiencies (e.g. vitamin A) are involved.

Ovulation influences bone metabolism in women undergoing controlled ovarian stimulation

2014 ◽  
Vol 122 (03) ◽  
Author(s):  
V Seifert-Klauss ◽  
AC Ritzinger ◽  
B Lesoine ◽  
M Kiechle ◽  
M Schmidmayr ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Ovarian Stimulation ◽  
Controlled Ovarian Stimulation
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