Green synthesis of Lawsonia inermis-mediated zinc ferrite nanoparticles for magnetic studies and anticancer activity against breast cancer (MCF-7) cell lines

Purpose. Histone modification has emerged as a promising approach to cancer therapy. The short-chain fatty acid, butyric acid, a histone deacetylase (HD) inhibitor, has shown anticancer activity. Butyrate transcriptional activation is indeed able to withdraw cancer cells from the cell cycle, leading to programmed cell death. Since butyrate’s clinical use is hampered by unfavorable pharmacokinetic and pharmacodynamic properties, delivery systems, such as solid lipid nanoparticles (SLN), have been developed to overcome these constraints. Methods. In order to outline the influence of butyrate delivery on its anticancer activity, the effects of butyrate as a free (sodium butyrate, NB) or nanoparticle (cholesteryl butyrate solid lipid nanoparticles, CBSLN) formulation on the growth of different human cancer cell lines, such as the promyelocytic leukemia, HL-60, and the breast cancer, MCF-7 was investigated. A detailed investigation into the mechanism of the induced cytotoxicity was also carried out, with a special focus on the modulation of HD and cyclin-dependent kinase (CDK) mRNA gene expression by real time PCR analysis. Results. In HL-60 cells, CBSLN induced a higher and prolonged expression level of the butyrate target genes at lower concentrations than NB. This led to a significant decrease in cell proliferation, along with considerable apoptosis, cell cycle block in the G0/G1 phase, significant inhibition of total HD activity and overexpression of the p21 protein. Conversely, in MCF-7 cells, CBSLN did not enhance the level of expression of the butyrate target genes, leading to the same anticancer activity as that of NB. Conclusions. Solid lipid nanoparticles were able to improve butyrate anticancer activity in HL-60, but not in MCF-7 cells. This is consistent with difference in properties of the cells under study, such as expression of the TP53 tumor suppressor, or the transporter for short-chain fatty acids, SLC5A8. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.Purpose. Histone modification has emerged as a promising approach to cancer therapy. The short-chain fatty acid, butyric acid, a histone deacetylase (HD) inhibitor, has shown anticancer activity. Butyrate transcriptional activation is indeed able to withdraw cancer cells from the cell cycle, leading to programmed cell death. Since butyrate’s clinical use is hampered by unfavorable pharmacological pharmacokinetic and pharmacodynamicproperties, delivery systems, such as solid lipid nanoparticles (SLN), have been developed to overcome these constraints. Methods. In order to outline the influence of butyrate delivery on its anticancer activity, the effects of butyrate as a free (sodium butyrate, NB) or nanoparticle (cholesteryl butyrate solid lipid nanoparticles, CBSLN) formulation was investigated on the growth of different human cancer cell lines, such as the promyelocytic leukemia, HL-60, and the breast cancer, MCF-7 was investigated. A detailed investigation into the mechanism of the induced cytotoxicity was also carried out, with a special focus on the modulation of HD and cyclin-dependent kinase (CDK) mRNA gene expression by real time PCR analysis. Results. In HL-60 cells, CBSLN induced a higher and prolonged expression level of the butyrate target genes at lower concentrations than NB. This led to a significant decrease in cell proliferation, along with considerable apoptosis, cell cycle block in the G0/G1 phase, significant inhibition of total HD activity and overexpression of the p21 protein. Conversely, in MCF-7 cells, CBSLN did not enhance the level of expression of the butyrate target genes, leading to the same anticancer activity as that of NB. Conclusions. Solid lipid nanoparticles were able to improve butyrate anticancer activity in HL-60, but not in MCF-7 cells. This is consistent with the difference in cells’ properties of the cells under study, such as expression of the TP53 tumor suppressor, or the transporter for short-chain fatty acids, SLC5A8. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page. <w:LsdException Locked="false"

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Anticancer Activity Of Silver Nanoparticles Synthesized Using Extract of Animal Waste As A Traditional Medicine On MCF-7 Human Breast Cancer Cell Line

10.21203/rs.3.rs-412407/v1 ◽

2021 ◽

Author(s):

Mohammad Mousavi-Khattat ◽

Hamid Nourbakhshan ◽

Mehrnaz Roumi ◽

Mahshid Ebrahiminejad ◽

Yasaman Fazeli ◽

...

Keyword(s):

Breast Cancer ◽

Silver Nanoparticles ◽

Green Synthesis ◽

Anticancer Activity ◽

Cell Line ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Mtt Assay ◽

Synthesis Methods ◽

Mcf 7

Abstract Green synthesis methods are environmentally friendly, cost effective and nonhazardous for biomedical applications in comparison with other methods. The aim of the study was green synthesis of silver nanoparticles using medicinal animal dung extract as a reducing, capping and stabilizing agent for the first time among other synthesis methods of silver nanoparticles. Female donkey’s dung was capable to reduce silver ions to nanoparticles and stabilize them. Silver nanoparticles with average sizes of 36 nm were synthesized and characterized by UV-Vis, FT-IR, XRD and TEM. Moreover, synthesized nanoparticles were analyzed in terms of anticancer activity by MTT assay on MCF-7 cell line. UV–Visible spectrophotometer showed an absorbance peak in the range of 414-433 nm. To identify the phytochemical coating of particles, FTIR analysis was used. Transmission electron microscope (TEM) images and X-ray diffraction (XRD) confirmed the formation of small spherical silver nanoparticles. The MTT assay revealed potent anticancer effects of the aqueous extract synthesized nanoparticles on MCF-7 cells, incubated for 24 hours. Based on the current findings, it is strongly believing that the use of donkey’s dung offers large scale production of biocompatible silver nanoparticles that can be suggested to possess valuable anticancer agents against breast cancer cell lines.

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Green Synthesis of Silver Nanoparticles Using Piper nigrum Concoction and its Anticancer Activity against MCF-7 and Hep-2 Cell Lines

Journal of Antimicrobial Agents ◽

10.4172/2472-1212.1000123 ◽

2016 ◽

Vol 2 (3) ◽

Cited By ~ 3

Author(s):

Manikandan E ◽

Krishnan V

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Anticancer Activity ◽

Cell Lines ◽

Piper Nigrum ◽

Mcf 7

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Novel Papaverine Metal Complexes with Potential Anticancer Activities

Molecules ◽

10.3390/molecules25225447 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5447

Author(s):

Ahmed Gaber ◽

Walaa F. Alsanie ◽

Deo Nandan Kumar ◽

Moamen S. Refat ◽

Essa M. Saied

Keyword(s):

Breast Cancer ◽

Biological Activity ◽

Metal Complexes ◽

Anticancer Activity ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Anticancer Activities ◽

Mcf 7

Cancer is one of the leading causes of death worldwide. Although several potential therapeutic agents have been developed to efficiently treat cancer, some side effects can occur simultaneously. Papaverine, a non-narcotic opium alkaloid, is a potential anticancer drug that showed selective antitumor activity in various tumor cells. Recent studies have demonstrated that metal complexes improve the biological activity of the parent bioactive ligands. Based on those facts, herein we describe the synthesis of novel papaverine–vanadium(III), ruthenium(III) and gold(III) metal complexes aiming at enhancing the biological activity of papaverine drug. The structures of the synthesized complexes were characterized by various spectroscopic methods (IR, UV–Vis, NMR, TGA, XRD, SEM). The anticancer activity of synthesized metal complexes was evaluated in vitro against two types of cancer cell lines: human breast cancer MCF-7 cells and hepatocellular carcinoma HepG-2 cells. The results revealed that papaverine-Au(III) complex, among the synthesized complexes, possess potential antimicrobial and anticancer activities. Interestingly, the anticancer activity of papaverine–Au(III) complex against the examined cancer cell lines was higher than that of the papaverine alone, which indicates that Au-metal complexation improved the anticancer activity of the parent drug. Additionally, the Au complex showed anticancer activity against the breast cancer MCF-7 cells better than that of cisplatin. The biocompatibility experiments showed that Au complex is less toxic than the papaverine drug alone with IC50 ≈ 111 µg/mL. These results indicate that papaverine–Au(III) complex is a promising anticancer complex-drug which would make it a suitable candidate for further in vivo investigations.

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DESIGN, SYNTHESIS, MOLECULAR DOCKING, AND BIOLOGICAL EVALUATION OF PYRAZOLE 1-CARBOTHIAMIDE INCORPORATED ISOXAZOLE DERIVATIVES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i5.32591 ◽

2019 ◽

pp. 245-250 ◽

Cited By ~ 1

Author(s):

RADHIKA TUMMA ◽

HARINADHA BABU VAMARAJU

Keyword(s):

Breast Cancer ◽

Anticancer Activity ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Antiproliferative Activity ◽

Active Site ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Mcf 7

Objectives: Novel isoxazole incorporated pyrazole carbothiamide 5 (a-r) was designed and synthesized, docked and evaluated for anticancer activity Michigan Cancer Foundation-7 (MCF-7), and breast cancer cell lines. Materials and Methods: Designed compounds were synthesized by the condensation of 1-(5-methyl-3-(4-nitrophenyl) isoxazole-4-yl) -3-(substitutedphenyl) prop-2-en-1-one (4) with thiosemicarbazides and substituted thiosemicarbazides to give the target molecules 5 (a-r). To predict the affinity and activity of the ligand molecule, the docking program Accelrys Discovery Studio 2.1 was employed to generate different bioactive binding poses of designing molecules at the active site of human Dihydrofolate Reductase (DHFR) (PDB ID: 1KMS). All the synthesized compounds were characterized based on the spectral and elemental analysis data. Antiproliferative activity was performed against MCF-7 breast cancer cell lines. Results: All the synthesized compounds showed the characteristic peaks in Fourier-transform infrared,1H C13NMR, and mass spectral analysis. During docking, all the synthesized compounds 5 (a-r) exhibited higher fitness scores with minimum three bonding interaction with the active site human DHFR (PDB ID: 1KMS). In the MTT assay based on MCF-7 breast cancer cell lines, most of the compounds exhibited significant activity. In the antiproliferative assay against MCF-7 cell lines, most of the compounds exhibited potent activity with IC50 values in micromolar concentrations. Compounds 5a, 5b, 5f, 5h, and 5k have exhibited significant anticancer activity. Conclusions: The derivatives were synthesized in quantitative yields. New derivatives possess the antiproliferative activity and antitubercular activity.

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