The effect of synthetic bone graft substitutes on bone formation in rabbit calvarial defects

AbstractThe aim of this study was to evaluate the influence of the intensity of the biomimetic hydroxyapatite (HA) coating of α-tricalcium phosphate (α-TCP) on biomaterial degradation and bone formation. Twenty-four female NZW rabbits of approximately 12 weeks of age were used. Critical size defects were randomly treated with 3%:97% HA:α-TCP (BBCP1), 12%:88% HA:α-TCP (BBCP2), and 23%:77% HA:α-TCP (BBCP3), respectively or sham. All defects were covered with a resorbable collagen membrane. Animals were euthanized after 3 and 12 weeks of healing and samples were investigated by micro-CT and histologic analysis. Ingrowth of newly formed woven bone from the original bone at 3-week healing period was observed in all samples. At the 12-week healing period, the new bone in the peripheral area was mainly lamellar and in the central region composed of both woven and lamellar bone. New bony tissue was found on the surface of all three types of granules and at the interior of the BBCP1 granules. Samples with 3% HA showed significantly less residual biomaterial in comparison to the other two groups. Furthermore, BBCP1 significantly promoted new bone area as compared to other three groups and more bone volume as compared to the control. Within its limitations, this study indicated the highest degradation rate in case of BBCP1 concomitant with the highest rate of bone formation. Hence, formation of new bone can be affected by the level of biomimetic HA coating of α-TCP.

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The Effects of Collagen Membrane Coated with PLGA on Bone Regeneration in Rat Calvarial Defects

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.357 ◽

2007 ◽

Vol 342-343 ◽

pp. 357-360

Author(s):

Kun Young Song ◽

Yoo Jung Um ◽

Ui Won Jung ◽

Yong Keun Lee ◽

Seong Ho Choi ◽

...

Keyword(s):

Bone Formation ◽

Bone Regeneration ◽

Collagen Membrane ◽

Histomorphometric Analysis ◽

Local Bone ◽

Calvarial Defects ◽

Formation Behavior ◽

Surgical Implantation ◽

Collagen Membranes ◽

Rat Calvarial Defect

The purpose of this study was to evaluate the effects of collagen membrane coated with PLGA on bone regeneration in rat calvarial defect. Five groups of 10 animals each received either collagen membrane coated with 0.5%, 1%, 3% concentration of PLGA, collagen membrane only or surgical control. Each group of animals was healed into 2 healing periods of 2(5 animals) and 8(5 animals)weeks and histologic and histomorphometric analysis were done. The results of the following study revealed that surgical implantation of collagen membranes coated with PLGA enhanced local bone formation at both 2 and 8 weeks independent of different PLGA concentrations. In conclusion, collagen membrane coated with PLGA shows a significant bone formation behavior irrespective of their concentration.

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Acid Dentin Lysate Failed to Modulate Bone Formation in Rat Calvaria Defects

Biology ◽

10.3390/biology10030196 ◽

2021 ◽

Vol 10 (3) ◽

pp. 196

Author(s):

Jila Nasirzade ◽

Karol Alí Apaza Alccayhuaman ◽

Zahra Kargarpour ◽

Ulrike Kuchler ◽

Franz Josef Strauss ◽

...

Keyword(s):

Computed Tomography ◽

Bone Formation ◽

Alveolar Bone ◽

Control Group ◽

Micro Computed Tomography ◽

Calvarial Bone ◽

Critical Size Defects ◽

Tooth Roots ◽

Calvarial Defects ◽

Collagen Membranes

Autogenous tooth roots are increasingly applied as a grafting material in alveolar bone augmentation. Since tooth roots undergo creeping substitution similar to bone grafts, it can be hypothesized that osteoclasts release the growth factors stored in the dentin thereby influencing bone formation. To test this hypothesis, collagen membranes were either soaked in acid dentin lysates (ADL) from extracted porcine teeth or serum–free medium followed by lyophilization. Thereafter, these membranes covered standardized 5-mm-diameter critical-size defects in calvarial bone on rats. After four weeks of healing, micro-computed tomography and histological analyses using undecalcified thin ground sections were performed. Micro-computed tomography of the inner 4.5 mm calvaria defects revealed a median bone defect coverage of 91% (CI: 87–95) in the ADL group and 94% (CI: 65–100) in the control group, without significant differences between the groups (intergroup p > 0.05). Furthermore, bone volume (BV) was similar between ADL group (5.7 mm3, CI: 3.4–7.1) and control group (5.7 mm3, CI: 2.9–9.7). Histomorphometry of the defect area confirmed these findings with bone area values amounting to 2.1 mm2 (CI: 1.2–2.6) in the ADL group and 2.0 mm2 (CI: 1.1–3.0) in the control group. Together, these data suggest that acid dentin lysate lyophilized onto collagen membranes failed to modulate the robust bone formation when placed onto calvarial defects.

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Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study

Biomedicines ◽

10.3390/biomedicines9020143 ◽

2021 ◽

Vol 9 (2) ◽

pp. 143

Author(s):

Hiroki Katagiri ◽

Yacine El Tawil ◽

Niklaus P. Lang ◽

Jean-Claude Imber ◽

Anton Sculean ◽

...

Keyword(s):

Bone Density ◽

Central Area ◽

Collagen Membrane ◽

Lamellar Bone ◽

Tissue Samples ◽

Critical Size Defects ◽

New Zealand White Rabbits ◽

Calvarial Defects ◽

Bone Height

The aim of this study was to evaluate the influence of additional hydroxyapatite (HA) in collagen-based matrices (CM) and membrane placement on bone formation in calvarial defects. Critical size defects in the calvaria of 16 New Zealand White Rabbits were randomly treated with CM or mineralized collagen-based matrices (mCM). Half of the sites were covered with a collagen membrane. Animals were euthanized after 12 weeks of healing. The samples were studied by micro-CT and histology. Newly formed lamellar bone was observed in all samples at the periphery of the defect. In the central areas, however, new bone composed of both woven and lamellar bone was embedded in the soft tissue. Samples treated with mCM showed more residual biomaterial and induced more small bony islands in the central areas of the defects than samples with CM. Nevertheless, a complete defect closure was not observed in any of the samples at 12 weeks. Membrane placement resulted in a decrease in bone density and height. Significant differences between the groups were revealed only between CM groups with and without membrane coverage for bone height in the central area of the defect. Neither mineralization of CM nor membrane placement improved the osteogenic capacity in this particular defect. Nevertheless, mineralisation influenced bone density without a membrane placement and bone volume underneath a membrane. CM may be used as a scaffold in bone regeneration procedures, without the need of a membrane coverage. Further preclinical studies are warrant to optimise the potential of mCM.

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Microtomography analysis of bone formation in calvarial defects filled with gelatin sponge or fibrin glue

Bone Abstracts ◽

10.1530/boneabs.1.pp63 ◽

2013 ◽

Author(s):

Santana Santos Thiago de ◽

Helena Bacha Lopes ◽

Adriana Luiza Almeida ◽

Marcio Mateus Beloti ◽

Adalberto Luiz Rosa

Keyword(s):

Bone Formation ◽

Fibrin Glue ◽

Gelatin Sponge ◽

Calvarial Defects

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Bone tissue engineering in the greater omentum with computer-aided design/computer-aided manufacturing scaffolds is enhanced by a periosteum transplant

Regenerative Medicine ◽

10.2217/rme-2020-0115 ◽

2020 ◽

Author(s):

Hendrik Naujokat ◽

Klaas Loger ◽

Juliane Schulz ◽

Yahya Açil ◽

Jörg Wiltfang

Keyword(s):

Bone Formation ◽

Bone Tissue ◽

Computer Aided Design ◽

Bone Tissue Regeneration ◽

Histological Evaluation ◽

Greater Omentum ◽

Collagen Membrane ◽

Computer Aided ◽

3D Printed ◽

The Impact

Aim: This study aimed to evaluate two different vascularized bone flap scaffolds and the impact of two barrier membranes for the reconstruction of critical-size bone defects. Materials & methods: 3D-printed scaffolds of biodegradable calcium phosphate and bioinert titanium were loaded with rhBMP-2 bone marrow aspirate, wrapped by a collagen membrane or a periosteum transplant and implanted into the greater omentum of miniature pigs. Results: Histological evaluation demonstrated significant bone formation within the first 8 weeks in both scaffolds. The periosteum transplant led to enhanced bone formation and a homogenous distribution in the scaffolds. The omentum tissue grew out a robust vascular supply. Conclusion: Endocultivation using 3D-printed scaffolds in the greater omentum is a very promising approach in defect-specific bone tissue regeneration.

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Maxillary Sinus Augmentation Using Ceramic Alloplastic Granules or Paste: An Experimental Study in Rabbits

Dentistry Journal ◽

10.3390/dj9060065 ◽

2021 ◽

Vol 9 (6) ◽

pp. 65

Author(s):

Michael Medeiros Costa ◽

Daniele Botticelli ◽

Ofer Moses ◽

Yuki Omori ◽

Shigeo Fujiwara ◽

...

Keyword(s):

Bone Formation ◽

Maxillary Sinus ◽

Biological Behavior ◽

Collagen Membrane ◽

Alloplastic Material ◽

New Bone Formation ◽

Sinus Augmentation ◽

Beta Tricalcium Phosphate ◽

Sinus Lifting ◽

Points Of View

Background: Due to the lack of data comparing the biological behavior of two formulations, granules and paste, of alloplastic graft from microtomographic and histomorphometric points of view, the aim of the present experiment was to compare the histomorphometric and microtomographic healing of two formulations, i.e., granules (MR sites) or paste (MR-inject sites) of an alloplastic graft composed of a combination of beta-tricalcium phosphate and hydroxyapatite used for maxillary sinus lifting. Methods: A sinus lifting procedure was carried out bilaterally in 20 rabbits, and the elevated space was filled with either paste or granules of an alloplastic material. A collagen membrane was placed on the antrostomy and the animals were euthanized after 2 or 10 weeks, 10 animals each group. Microtomographic and histological analyses were performed. Results: Higher proportions of new bone formation were found at the MR, compared to the MR-inject sites both after 2 weeks (2.65 ± 2.89% vs. 0.08 ± 0.12%; p < 0.01) and 10 weeks of healing (34.20 ± 13.86 vs. 23.28 ± 10.35%; p = 0.022). Conclusions: It was concluded that new bone formation was faster in the MR sites, compared to the MR-inject. However, a longer time of healing should be allowed to make final conclusions about the efficiency in bone formation of the paste formulation of the biomaterial used in the present study.

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