Bone tissue engineering in the greater omentum with computer-aided design/computer-aided manufacturing scaffolds is enhanced by a periosteum transplant

2020 ◽  
Author(s):  
Hendrik Naujokat ◽  
Klaas Loger ◽  
Juliane Schulz ◽  
Yahya Açil ◽  
Jörg Wiltfang
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Computer Aided Design ◽  
Bone Tissue Regeneration ◽  
Histological Evaluation ◽  
Greater Omentum ◽  
Collagen Membrane ◽  
Computer Aided ◽  
3D Printed ◽  
The Impact

Aim: This study aimed to evaluate two different vascularized bone flap scaffolds and the impact of two barrier membranes for the reconstruction of critical-size bone defects. Materials & methods: 3D-printed scaffolds of biodegradable calcium phosphate and bioinert titanium were loaded with rhBMP-2 bone marrow aspirate, wrapped by a collagen membrane or a periosteum transplant and implanted into the greater omentum of miniature pigs. Results: Histological evaluation demonstrated significant bone formation within the first 8 weeks in both scaffolds. The periosteum transplant led to enhanced bone formation and a homogenous distribution in the scaffolds. The omentum tissue grew out a robust vascular supply. Conclusion: Endocultivation using 3D-printed scaffolds in the greater omentum is a very promising approach in defect-specific bone tissue regeneration.

scholarly journals Rapid Prototyping Assisted Scaffold Fabrication for Bone Tissue Regeneration

2016 ◽  
Vol 5 (4) ◽  
pp. 79 ◽  
Author(s):  
Pranav S. Sapkal ◽  
Shraddha Jaiswal ◽  
Abhaykumar M. Kuthe
Keyword(s):  
Cell Culture ◽  
Rapid Prototyping ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Computer Aided Design ◽  
Organ Transplant ◽  
Bone Tissue Regeneration ◽  
Patient Specific ◽  
Scaffold Fabrication ◽  
Computer Aided

<p class="1Body">The review article focuses on Rapid Prototyped assisted scaffold fabrication for bone tissue regeneration, particularly in respect of its mechanical properties and cell culture abilities. The distinct feature of computer aided design and computer aided manufacturing (CAD &amp; CAM), imaging technology and rapid prototyping (RP) technology has been used by different researchers to print porous scaffolds with requisite shape and interconnected channels for osseous tissue formation. This study concludes that the use of RP in scaffold manufacturing offers patient specific designed scaffolds<strong> </strong>with improved strength, in-vitro and in-vivo cell culture capability unlike traditional scaffold fabrication techniques. Tissue engineering using 3D Printing is a viable substitute for organ transplant, which<strong> </strong>requires willing donors to part with their organs. This study reviewed the benefits of RP/imaging/CAD-CAM to develop scaffolds for bone<strong> </strong>tissue regeneration and it serves those patients who could not be accurately treated by traditional means. The article is helpful to study the influence of RP in the field of organ transplant</p>

Engineering 3D printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration

2021 ◽  
Vol 122 ◽  
pp. 111928
Author(s):  
Eduardo H. Backes ◽  
Emanuel M. Fernandes ◽  
Gabriela S. Diogo ◽  
Catarina F. Marques ◽  
Tiago H. Silva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Calcium Phosphates ◽  
Bone Tissue Regeneration ◽  
Aliphatic Polyester ◽  
Composite Scaffolds ◽  
3D Printed

Redefining architectural effects in 3D printed scaffolds through rational design for optimal bone tissue regeneration

2021 ◽  
Vol 25 ◽  
pp. 101168
Author(s):  
Mohammad Mirkhalaf ◽  
Xiao Wang ◽  
Ali Entezari ◽  
Colin R. Dunstan ◽  
Xinquan Jiang ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Rational Design ◽  
Bone Tissue Regeneration ◽  
3D Printed ◽  
Architectural Effects

Effect of In-office Bleaching on Color, Translucency, and Whiteness Variations in CAD-CAM Monolithic Materials

2021 ◽  
Author(s):  
RG Fonseca ◽  
RC Peńa ◽  
R Simóes ◽  
AC Ramos ◽  
LN Dovigo
Keyword(s):  
Optical Properties ◽  
Computer Aided Design ◽  
Calculated Data ◽  
Color Difference ◽  
Deionized Water ◽  
Stain Removal ◽  
Computer Aided ◽  
Cad Cam ◽  
Aided Design ◽  
The Impact

SUMMARY Little is known about the impact of bleaching on the optical properties of computer-aided design and computer-aided manufactured (CAD-CAM) monolithic materials. The aim of the present study was to evaluate the effect of one session of in-office bleaching on stain removal, staining susceptibility, translucency, and whiteness variations of CADCAM monolithic materials. Disks were fabricated from Lava Ultimate (LU), Vita Enamic (VE), Vita Suprinity (VS), and IPS e.max CAD (IPS). A spectrophotometer was used to register Commission Internationale de l’Eclairage L*a*b* coordinates. For stain removal, 80 specimens from each material were assessed at baseline (R0) and after immersion in deionized water or coffee for 365 days followed or not by bleaching with 40% hydrogen peroxide (R1). For staining susceptibility, 80 specimens from each material were analyzed at baseline (R0’), and after having been bleached or not and immersed in deionized water or coffee (R1’). Both analyses were calculated as the color difference (ΔE00) between R1-R0 and R1’-R0’, respectively. Differences in translucency (ΔTP00) and whiteness (ΔWID) between R1-R0 and R1’-R0’ were also calculated. Data were analyzed by three-way ANOVA and the Games-Howell post hoc test (α=0.05). Clinical significance was based on 50%:50% perceptibility and acceptability thresholds for ΔE00, ΔTP00 and ΔWID, respectively. Surfaces were analyzed by scanning electron microscopy. Coffee increased ΔE00 in LU, VE, and VS, and decreased their translucency and whiteness, whereas the IPS had only its whiteness affected. Bleaching after immersion in coffee decreased ΔE00 in LU and VE, and increased translucency and whiteness of LU, VE, and VS. No effect was observed on IPS. Bleaching before immersion in coffee decreased translucency of LU, but within the acceptable interval, while VE exhibited lower ΔE00, and became more translucent and less dark. Both VS and IPS were not affected. One session of in-office bleaching benefited optical properties of the previously stained LU, VE, and VS, without increasing their susceptibility to staining or adversely providing clinically unacceptable variations in their translucency and whiteness. All variations exhibited by the IPS were below the perceptible threshold.

scholarly journals Application Solid Laser-Sintered or Machined Ti6Al4V Alloy in Manufacturing of Dental Implants and Dental Prosthetic Restorations According to Dentistry 4.0 Concept

Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 664 ◽  
Author(s):  
Leszek A. Dobrzański ◽  
Lech B. Dobrzański ◽  
Anna Achtelik-Franczak ◽  
Joanna Dobrzańska
Keyword(s):  
Dental Implants ◽  
Computer Aided Design ◽  
Bending Strength ◽  
Ti6al4v Alloy ◽  
Cnc Machining ◽  
Live Cells ◽  
Computer Aided ◽  
Aided Design ◽  
The Impact

This paper presents a comparison of the impact of milling technology in the computer numerically controlled (CNC) machining centre and selective laser sintering (SLS) and on the structure and properties of solid Ti6Al4V alloy. It has been shown that even small changes in technological conditions in the SLS manufacturing variant significantly affect changes from two to nearly two and a half times in tensile and bending strengths. Both the tensile and bending strength obtained in the most favourable manufacturing variant by the SLS method is over 25% higher than in the case of cast materials subsequently processed by milling. Plug-and-play SLS conditions provide about 60% of the possibilities. Structural, tribological and electrochemical tests were carried out. In vitro biological tests using osteoblasts confirm the good tendency for the proliferation of live cells on the substrate manufactured under the most favourable SLS conditions. The use of SLS additive technology for the manufacturing of dental implants and abutments made of Ti6Al4V alloy in combination with the digitisation of dental diagnostics and computer-aided design and manufacture of computer-aided design/manufacturing (CAD/CAM) following the idea of Dentistry 4.0 is the best choice of technology for manufacturing of prosthetic and implant devices used in dentistry.

scholarly journals 3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration

2019 ◽  
Vol 20 (4) ◽  
pp. 942 ◽  
Author(s):  
Yuan-Haw Wu ◽  
Yung-Cheng Chiu ◽  
Yen-Hong Lin ◽  
Chia-Che Ho ◽  
Ming-You Shie ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Calcium Silicate ◽  
Interleukin 1 ◽  
Orthopedic Implants ◽  
Cellular Adhesion ◽  
Bone Tissue Regeneration ◽  
Necrosis Factor Alpha ◽  
Mg63 Cells ◽  
3D Printed

Currently, clinically available orthopedic implants are extremely biocompatible but they lack specific biological characteristics that allow for further interaction with surrounding tissues. The extracellular matrix (ECM)-coated scaffolds have received considerable interest for bone regeneration due to their ability in upregulating regenerative cellular behaviors. This study delves into the designing and fabrication of three-dimensional (3D)-printed scaffolds that were made out of calcium silicate (CS), polycaprolactone (PCL), and decellularized ECM (dECM) from MG63 cells, generating a promising bone tissue engineering strategy that revolves around the concept of enhancing osteogenesis by creating an osteoinductive microenvironment with osteogenesis-promoting dECM. We cultured MG63 on scaffolds to obtain a dECM-coated CS/PCL scaffold and further studied the biological performance of the dECM hybrid scaffolds. The results indicated that the dECM-coated CS/PCL scaffolds exhibited excellent biocompatibility and effectively enhanced cellular adhesion, proliferation, and differentiation of human Wharton’s Jelly mesenchymal stem cells by increasing the expression of osteogenic-related genes. They also presented anti-inflammatory characteristics by showing a decrease in the expression of tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). Histological analysis of in vivo experiments presented excellent bone regenerative capabilities of the dECM-coated scaffold. Overall, our work presented a promising technique for producing bioscaffolds that can augment bone tissue regeneration in numerous aspects.

Ornamenting 3D printed scaffolds with cell-laid extracellular matrix for bone tissue regeneration

Biomaterials ◽  
2015 ◽  
Vol 37 ◽  
pp. 230-241 ◽  
Author(s):  
Falguni Pati ◽  
Tae-Ha Song ◽  
Girdhari Rijal ◽  
Jinah Jang ◽  
Sung Won Kim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
3D Printed
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