Effect of γ-Al2O3 Nanostructure Surface Morphology on Lipid Peroxidation and Cathepsin Activity in Neuro-2a Neuroblastoma Cells

2022 ◽  
Author(s):  
Ludmila V. Spirina ◽  
Olga Bakina ◽  
Sergey O. Kazantsev ◽  
Alexandra V. Avgustinovich ◽  
Sergey G. Afanas’ev
Keyword(s):  
Lipid Peroxidation ◽  
Surface Morphology ◽  
Neuroblastoma Cells ◽  
Cathepsin Activity ◽  
Nanostructure Surface

scholarly journals NEUROPROTECTIVE EFFECT OF ARTOCARPUS LAKOOCHA EXTRACT AND OXYRESVERATROL AGAINST HYDROGEN PEROXIDE-INDUCED TOXICITY IN SH-SY5Y CELLS

2017 ◽  
Vol 9 (10) ◽  
pp. 229 ◽  
Author(s):  
Hasriadi . ◽  
Matusorn Wong-on ◽  
Phakhamon Lapphanichayakool ◽  
Nanteetip Limpeanchob
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Cell Viability ◽  
Mitochondrial Function ◽  
Neuroprotective Effect ◽  
Neuroblastoma Cells ◽  
Effective Protection ◽  
Intracellular Ros ◽  
Medical Plant ◽  
Continuous Presence

Objective: Artocarpus lakoocha Roxb. is a traditional medical plant native to Southeast Asia and used as a dried aqueous extract so-called puag haad. Its role (and its major ingredient, oxyresveratrol) as an antioxidant neuroprotectant were explored.Methods: Differentiated SH-SY5Y neuroblastoma cells in 96-well plates were challenged with 200 µM H2O2 for 4 h and either Trolox (100 µM), oxyresveratrol (5-100 µM), or puag haad (1.2-25 µg/ml) applied 2 h before H2O2 or for 20 h after H2O2 washout. Cell viability, mitochondrial function, intracellular ROS, and lipid peroxidation were assessed.Results: Continuous presence of both H2O2 and antioxidant reduced mitochondrial function by ~50% but only by 30% with antioxidant. Sustained 24 h H2O2 showed no recoveries with antioxidants. Cell viability was modestly restored when antioxidants accompanied H2O2 for 4 h and both washed for another 20 h, but little recovery of mitochondrial function even though antioxidants removed ROS and prevent lipid peroxidation. Antioxidants added for 20 h after H2O2 marginally improve mitochondria and modestly restore cell viability, but lipid peroxidation was completely reversed.Conclusion: These results show that mitochondrial protection was illusive, yet both tested compounds, puag haad and oxyresveratrol, improved cell viability and especially ROS levels and lipid peroxidation. The potency oxyresveratrol on the redox-sensitive expression of antioxidant enzymes and its pharmacokinetics suggests that oral puag haad could provide effective protection in transient neurodegenerative disease. 

Ascorbate and iron increases lipid peroxidation in melanoma- and neuroblastoma cells

1993 ◽  
Vol 3 ◽  
pp. 39
Author(s):  
B. Schaber ◽  
S. Peter ◽  
P. Mayer ◽  
G. Rassner ◽  
G. Fierlbeck
Keyword(s):  
Lipid Peroxidation ◽  
Neuroblastoma Cells

Effects of Carbon Tetrachloride on Perfused Cultures of Hepatic and Neuronal Cells

1992 ◽  
Vol 20 (2) ◽  
pp. 235-239
Author(s):  
Stefan Wallin ◽  
Erik Walum
Keyword(s):  
Lipid Peroxidation ◽  
Carbon Tetrachloride ◽  
Cytochrome C ◽  
Membrane Permeability ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Neuronal Cells ◽  
Neuroblastoma Cells ◽  
Cellular Respiration ◽  
Static Conditions

Cultured hepatocytes and hemisphere neurons from chick embryos and mouse neuroblastoma cells were exposed to carbon tetrachloride (CC14; 0, 1, 2, 3 and 4mM) for 1 hour, using a perfusion system developed for studying the effects of volatile substances. In the perfused cultures, three parameters were compared: lipid peroxidation, membrane integrity and cellular respiration. In addition, cytochrome C oxidase activity was determined after incubation of cell homogenates with CC14. A concentration-dependent increase in lipid peroxidation and membrane permeability was found in the neuroblastoma cells. The hepatocytes responded to a lesser extent with respect to membrane permeability and their lipid peroxidation did not differ from that of controls. The hepatocytes responded with a 35% decrease in respiration when exposed to 3mM CC14, and a 20% decrease in cytochrome C oxidase activity after treatment with 1.5mM CCl4. In the neuronal cells, much smaller decreases in respiration were found and their cytochrome C oxidase activity remained unaffected. These results are very similar to those obtained after incubation in a closed chamber system. However, the perfused cells were found to be less sensitive to CCl4 than cells exposed under static conditions.

Clusterin up-regulation following sub-lethal oxidative stress and lipid peroxidation in human neuroblastoma cells

2006 ◽  
Vol 27 (11) ◽  
pp. 1588-1594 ◽  
Author(s):  
Paola Strocchi ◽  
Mark A. Smith ◽  
George Perry ◽  
Elena Tamagno ◽  
Oliviero Danni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

Specimen Collection Effect in Scanning Electron Microscopy Images

1972 ◽  
Vol 30 ◽  
pp. 448-449
Author(s):  
J. Temple Black ◽  
Jose Guerrero
Keyword(s):  
Surface Morphology ◽  
Secondary Electron Emission ◽  
Secondary Electrons ◽  
Charge Effects ◽  
Material Density ◽  
Specimen Collection ◽  
The Subject ◽  
Curved Paths ◽  
Subject Material ◽  
Microscopy Images

In the SEM, contrast in the image is the result of variations in the volume secondary electron emission and backscatter emission which reaches the detector and serves to intensity modulate the signal for the CRT's. This emission is a function of the accelerating potential, material density, chemistry, crystallography, local charge effects, surface morphology and especially the angle of the incident electron beam with the particular surface site. Aside from the influence of object inclination, the surface morphology is the most important feature In producing contrast. “Specimen collection“ is the name given the shielding of the collector by adjacent parts of the specimen, producing much image contrast. This type of contrast can occur for both secondary and backscatter electrons even though the secondary electrons take curved paths to the detector-collector.Figure 1 demonstrates, in a unique and striking fashion, the specimen collection effect. The subject material here is Armco Iron, 99.85% purity, which was spark machined.

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