scholarly journals Microbial Volatile Organic Compounds from Tempered and Incubated Grain Mediate Attraction by a Primary but Not Secondary Stored Product Insect Pest in Wheat

Author(s):  
Taylor Van Winkle ◽  
Marco Ponce ◽  
Hannah Quellhorst ◽  
Alexander Bruce ◽  
Chloe E. Albin ◽  
...  

AbstractThere has been a dearth of research elucidating the behavioral effect of microbially-produced volatile organic compounds on insects in postharvest agriculture. Demonstrating attraction to MVOC’s by stored product insects would provide an additional source of unique behaviorally-relevant stimuli to protect postharvest commodities at food facilities. Here, we assessed the behavioral response of a primary (Rhyzopertha dominica) and secondary (Tribolium castaneum) grain pest to bouquets of volatiles produced by whole wheat that were untempered, or tempered to 12%, 15%, or 19% grain moisture and incubated for 9, 18, or 27 days. We hypothesized that MVOC’s may be more important for the secondary feeder because they signal that otherwise unusable, intact grains have become susceptible by weakening of the bran. However, contrary to our expectations, we found that the primary feeder, R. dominica, but not T. castaneum was attracted to MVOC’s in a wind tunnel experiment, and in a release-recapture assay using commercial traps baited with grain treatments. Increasing grain moisture resulted in elevated grain damage detected by near-infrared spectroscopy and resulted in small but significant differences in the blend of volatiles emitted by treatments detected by gas chromatography coupled with mass spectrometry (GC–MS). In sequencing the microbial community on the grain, we found a diversity of fungi, suggesting that an assemblage was responsible for emissions. We conclude that R. dominica is attracted to a broader suite of MVOC’s than T. castaneum, and that our work highlights the importance of understanding insect-microbe interactions in the postharvest agricultural supply chain.

2010 ◽  
Vol 44 (30) ◽  
pp. 3651-3659 ◽  
Author(s):  
Jaana Bäck ◽  
Hermanni Aaltonen ◽  
Heidi Hellén ◽  
Maija K. Kajos ◽  
Johanna Patokoski ◽  
...  

2018 ◽  
Vol 254 ◽  
pp. 597-602 ◽  
Author(s):  
Felix Y.H. Kutsanedzie ◽  
Lin Hao ◽  
Song Yan ◽  
Qin Ouyang ◽  
Quansheng Chen

Author(s):  
Jeevanandam Vaishnavi ◽  
Swarnkumar Reddy ◽  
Santhanam Narmadha ◽  
W. Jabez Osborne

2019 ◽  
Vol 95 (8) ◽  
Author(s):  
Wietse de Boer ◽  
Xiaogang Li ◽  
Annelein Meisner ◽  
Paolina Garbeva

ABSTRACT There is increasing evidence that microbial volatile organic compounds (mVOCs) play an important role in interactions between microbes in soils. In this minireview, we zoom in on the possible role of mVOCs in the suppression of plant-pathogenic soil fungi. In particular, we have screened the literature to see what the actual evidence is that mVOCs in soil atmospheres can contribute to pathogen suppression. Furthermore, we discuss biotic and abiotic factors that influence the production of suppressive mVOCs in soils. Since microbes producing mVOCs in soils are part of microbial communities, community ecological aspects such as diversity and assembly play an important role in the composition of produced mVOC blends. These aspects have not received much attention so far. In addition, the fluctuating abiotic conditions in soils, such as changing moisture contents, influence mVOC production and activity. The biotic and abiotic complexity of the soil environment hampers the extrapolation of the production and suppressing activity of mVOCs by microbial isolates on artificial growth media. Yet, several pathogen suppressive mVOCs produced by pure cultures do also occur in soil atmospheres. Therefore, an integration of lab and field studies on the production of mVOCs is needed to understand and predict the composition and dynamics of mVOCs in soil atmospheres. This knowledge, together with the knowledge of the chemistry and physical behaviour of mVOCs in soils, forms the basis for the development of sustainable management strategies to enhance the natural control of soil-borne pathogens with mVOCs. Possibilities for the mVOC-based control of soil-borne pathogens are discussed.


Author(s):  
Dalma Radványi ◽  
András Geösel ◽  
Zsuzsa Jókai ◽  
Péter Fodor ◽  
Attila Gere

Button mushrooms are one of the most commonly cultivated mushroom species facing different risks e.g.: viral, bacterial and fungal diseases. One of the most common problems is caused by Trichoderma aggressivum, or ‘green mould' disease. The presence or absence of mushroom disease-related moulds can sufficiently be detected from the air by headspace solid-phase microextraction coupled gas chromatography-mass spectrometry (HS SPME GC-MS) via their emitted microbial volatile organic compounds (MVOCs). In the present study, HS SPME GC-MS was used to explore the volatile secondary metabolites released by T. aggressivum f. europaeum on different nutrient-rich and -poor media. The MVOC pattern of green mould was determined, then media-dependent and independent biomarkers were also identified during metabolomic experiments. The presented results provide the basics of a green mould identification system which helps producers reducing yield loss, new directions for researchers in mapping the metabolomic pathways of T. aggressivum and new tools for policy makers in mushroom quality control.


2018 ◽  
Vol 52 (15) ◽  
pp. 8272-8282 ◽  
Author(s):  
Pawel K. Misztal ◽  
Despoina S. Lymperopoulou ◽  
Rachel I. Adams ◽  
Russell A. Scott ◽  
Steven E. Lindow ◽  
...  

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