Surface functionalization of two-photon dye-doped mesoporous silica nanoparticles with folic acid: cytotoxicity studies with HeLa and MCF-7 cancer cells

This work presents the synthesis of pH-responsive poly(2-(diethylamino) ethyl methacrylate) (PDEAEMA) brushes anchored on hollow mesoporous silica nanoparticles (HMSN-PDEAEMA) via a surface-initiated ARGET ATRP technique. The average size of HMSNs was ca. 340 nm, with a 90 nm mesoporous silica shell. The dry thickness of grafted PDEAEMA brushes was estimated to be ca 30 nm, as estimated by SEM and TEM. The halogen group on the surface of PDEAMA brushes was successfully derivatized with glucosamine, as confirmed by XPS. The effect of pH on the size of the hybrid nanoparticles was investigated by DLS. The size of fabricated nanoparticle decreased from ca. 950 nm in acidic media to ca. 500 nm in basic media due to the deprotonation of tertiary amine in the PDEAEMA. The PDEAEMA modified HMSNs nanocarrier was efficiently loaded with doxorubicin (DOX) with a loading capacity of ca. 64%. DOX was released in a relatively controlled pH-triggered manner from hybrid nanoparticles. The cytotoxicity studies demonstrated that DOX@HMSN-PDEAEMA-Glucosamine showed a strong ability to kill breast cancer cells (MCF-7 and MCF-7/ADR) at low drug concentrations, in comparison to free DOX.

Download Full-text

Folic Acid-Targeted Mesoporous Silica Nanoparticles for Two-Photon Fluorescence

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2010.1112 ◽

2010 ◽

Vol 6 (2) ◽

pp. 176-180 ◽

Cited By ~ 38

Author(s):

Valérie Lebret ◽

Laurence Raehm ◽

Jean-Olivier Durand ◽

Monique Smaïhi ◽

Martinus H. V. Werts ◽

...

Keyword(s):

Folic Acid ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Two Photon ◽

Two Photon Fluorescence ◽

Photon Fluorescence

Download Full-text

Aptamer-Based electrochemiluminescent detection of MCF-7 cancer cells based on carbon quantum dots coated mesoporous silica nanoparticles

Electrochimica Acta ◽

10.1016/j.electacta.2014.08.129 ◽

2014 ◽

Vol 146 ◽

pp. 262-269 ◽

Cited By ~ 43

Author(s):

Min Su ◽

Heng Liu ◽

Lei Ge ◽

Yanhu Wang ◽

Shenguang Ge ◽

...

Keyword(s):

Quantum Dots ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Cancer Cells ◽

Mesoporous Silica Nanoparticles ◽

Carbon Quantum Dots ◽

Mcf 7

Download Full-text

Effect of Surface Functionalization of MCM-41-Type Mesoporous Silica Nanoparticles on the Endocytosis by Human Cancer Cells

Journal of the American Chemical Society ◽

10.1021/ja0645943 ◽

2006 ◽

Vol 128 (46) ◽

pp. 14792-14793 ◽

Cited By ~ 626

Author(s):

Igor Slowing ◽

Brian G. Trewyn ◽

Victor S.-Y. Lin

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Cancer Cells ◽

Surface Functionalization ◽

Human Cancer ◽

Mesoporous Silica Nanoparticles ◽

Human Cancer Cells ◽

Mcm 41 ◽

Effect Of Surface

Download Full-text

Differently sized drug-loaded mesoporous silica nanoparticles elicit differential gene expression in MCF-7 cancer cells

Nanomedicine ◽

10.2217/nnm-2020-0375 ◽

2021 ◽

Author(s):

Baranya Murugan ◽

Uma Maheswari Krishnan

Keyword(s):

Gene Expression ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Cancer Cells ◽

Mesoporous Silica Nanoparticles ◽

Sol Gel ◽

Anticancer Efficacy ◽

Mcf 7

Aim: This study investigates the effects of different sized unmodified and chemo-responsive mesoporous silica nanocarriers on MCF-7 cancer cells. Materials & methods: Unmodified and thiol-functionalized large and small-sized mesoporous MCM-41 silica nanoparticles prepared using templated sol-gel process were characterized for their physicochemical properties and in vitro and in vivo anticancer efficacy. Microarray analysis was carried out to assess their differential effect on gene expression. Results: Thiol-functionalized nanoparticles displayed chemo responsive release and greater cytotoxicity to cancer cells when compared with unmodified carriers. Microarray studies showed distinct differences in genes differentially regulated by sMCM-41and lMCM-41 carriers when compared with the free drug. Conclusion: The small chemo-responsive carrier was more effective in suppressing oncogenes and genes involved in proliferation, invasion and survival while the large carrier mainly altered membrane-associated pathways.

Download Full-text