Effects of vitamin A and vitamin E on attenuation of titanium dioxide nanoparticles-induced toxicity in the liver of male Wistar rats

Titanium dioxide nanoparticles (TiO2-NPs) is widely applicated as additives in foods for its excellent whitening and brightening capability. Although the toxicity and antibacterial activity of TiO2-NPs has been extensively studied,...

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Effect of vitamin A and vitamin C on attenuation of ivermectin-induced toxicity in male Wistar rats

Environmental Science and Pollution Research ◽

10.1007/s11356-018-2961-7 ◽

2018 ◽

Vol 25 (29) ◽

pp. 29408-29417 ◽

Cited By ~ 4

Author(s):

Fatemeh Sadat Hosseini Omshi ◽

Roghayeh Abbasalipourkabir ◽

Maliheh Abbasalipourkabir ◽

Sedigheh Nabyan ◽

Alireza Bashiri ◽

...

Keyword(s):

Vitamin A ◽

Vitamin C ◽

Wistar Rats ◽

Male Wistar Rats

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Effectiveness of Vitamin C and Vitamin E Antioxidant Combination on Caspase-3 Expression in Wistar White Rat Pulmonum Contusion

Sriwijaya Journal of Surgery ◽

10.37275/sjs.v3i1.26 ◽

2020 ◽

Vol 3 (1) ◽

pp. 31-44

Author(s):

Bermansyah ◽

Gama Satria ◽

Ahmad Umar

Keyword(s):

Cell Death ◽

Vitamin E ◽

Vitamin C ◽

Wistar Rats ◽

Caspase 3 ◽

Cell Function ◽

Pulmonary Contusion ◽

Tnf Α ◽

Male Wistar Rats

Introduction.Pulmonary contusions can cause a progressive inflammatory response. Activation of TNF-α cytokines and reactive oxygen species (ROS) can cause pulmonary cell death. Antioxidants can have the potential to neutralize ROS. The purpose of this study is to determine the effectiveness of antioxidant administration in maintaining pulmonary cell function in wistar rats that have been induced to experience pulmonary contusions through caspase-3 levels. Methods.This study was an in vivo experimental study conducted on thirty male wistar rats and divided into five groups (n = 6): control, pulmonary contusion + asthaxanthine 5 mg/kgBW, pulmonary contusion + vitamin C and E 50 mg/kgBW, pulmonary contusion + vitamin C and E 100 mg/kgBW, pulmonary contusion + vitamin C and E 200 mg/kgBW. The value of Caspase-3 is evaluated by the IHC. All data analyzes used SPSS 18. Results. Low doses of antioxidants have the potential to reduce pulmonary cell death in wistar rats induced by pulmonary contusions.Conclussion. Vitamin C and E effective to reduce polmonary cell death in pulmonary contusion.Keywords: antioxidants, vitamin C, vitamin E, pulmonary contusions animal model, apoptosis, caspase-3

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Vitamin A deficiency regulates the expression of ferritin in young male Wistar rats

Revista de Nutrição ◽

10.1590/1678-9865202134e200297 ◽

2021 ◽

Vol 34 ◽

Author(s):

Mauricio RESTREPO-GALLEGO ◽

Luis Eduardo DÍAZ ◽

Juan David OSPINA-VILLA ◽

Danny CHINCHILLA-CÁRDENAS

Keyword(s):

Iron Deficiency ◽

Vitamin A ◽

Biochemical Parameters ◽

Serum Iron ◽

Wistar Rats ◽

Vitamin A Deficiency ◽

Young Male ◽

Control Group ◽

Transferrin Receptors ◽

Male Wistar Rats

ABSTRACT Objective Iron deficiency and vitamin A deficiency are two of the main micronutrient deficiencies. Both micronutrients are essential for human life and children's development. This study aimed to investigate the effects of vitamin A deficiency on ferritin and transferrin receptors' expression and its relationship with iron deficiency. Methods Five diets with different vitamin A-to-iron ratios were given to thirty five 21-day-old male Wistar rats (separated in groups of seven animals each). The animals received the diet for six weeks before being euthanized. Serum iron and retinol levels were measured as biochemical parameters. Their duodenums, spleens, and livers were analyzed for the expression of ferritin and transferrin receptors by Western Blotting. Results Regarding biochemical parameters, the results show that when both vitamin A and iron are insufficient, the serum iron content (74.74µg/dL) is significantly lower than the control group (255.86µg/dL). The results also show that vitamin A deficiency does not influence the expression of the transferrin receptor, but only of the ferritin one. Conclusion Vitamin A deficiency regulates the expression of ferritin in young male Wistar rats.

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Vitamin A does not influence mRNA expression of hormone hepcidin but other biomarkers of iron homeostasis in young male Wistar rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000666 ◽

2020 ◽

pp. 1-8

Author(s):

Mauricio Restrepo-Gallego ◽

Luis E. Díaz

Keyword(s):

Iron Deficiency ◽

Vitamin A ◽

Mrna Expression ◽

Wistar Rats ◽

Iron Homeostasis ◽

Regulatory Protein ◽

Deficiency Anemia ◽

Control Group ◽

Dietary Iron ◽

Male Wistar Rats

Abstract. The effects of an adequate supply of vitamin A and iron, in comparison with diets low or absent in vitamin A and low in iron, on the mRNA expression of some biomarkers of iron homeostasis as hepcidin ( Hamp), transferrin receptor-1 ( Tfrc), iron regulatory protein-2 ( Ireb2) and ferritin ( Fth1) in rats were investigated. 35 male Wistar rats were randomly divided into 5 dietary groups: control, sufficient in iron and insufficient in vitamin A (FesvAi), sufficient in iron and depleted in vitamin A (FesvAd), insufficient in iron and sufficient in vitamin A (FeivAs) and insufficient in both iron and vitamin A (FeivAi). After 6 weeks rats showed no significant effects of variations in vitamin A on the expression of Hamp relative to the control group (FesvAi: 1.37-fold; FesvAd: 1.22-fold); however, iron deficiency showed significant reduction on it relative to the control group (FeivAs: 71.4-fold, P = 0.0004; FeivAi: 16.1-fold, P = 0.0008). Vitamin A deficiency (FesvAd) affects expression of Fth1 independent of low dietary iron in spleen (0.29-fold, P = 0.002) and duodenum (5.15-fold, P = 0.02). Variations of dietary iron and vitamin A showed significant effects relative to the control group for expression of Tfrc in spleen (FesvAd: 0.18-fold, P = 0.01; FeivAs: 0.24-fold, P < 0.0001; FeivAi: 0.42-fold, P = 0.014), Ireb2 in spleen (FeivAs: 3.7-fold, P < 0.0001; FeivAi: 2.9-fold, P < 0.0001) and Ireb2 in duodenum (FeivAs: 2.68-fold, P = 0.012; FeivAi: 2.60-fold, P = 0.014). These results show that vitamin A and iron must be supplied together to regulate some of the main biomarkers of iron metabolism as a strategy to reduce prevalence of iron deficiency anemia.

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Reduction of Locomotor and Brain Activity of Wistar Rats after Serial Administration of Titanium Dioxide

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1085.400 ◽

2015 ◽

Vol 1085 ◽

pp. 400-405

Author(s):

Marina Khodanovich ◽

Anna Zelenskaya ◽

Elizaveta Gul ◽

Dmitry Sukhanov ◽

Elena Krutenkova

Keyword(s):

Particle Size ◽

Titanium Dioxide ◽

Wistar Rats ◽

Neuronal Damage ◽

Titanium Dioxide Nanoparticles ◽

Brain Activity ◽

Open Field Behavior ◽

Low Frequencies ◽

The Central Nervous System ◽

Electroencephalogram Eeg

Nanoparticles of titanium dioxide (TiO2) are widely used nanomaterial with particle size below 100 nanometers TiO2 is applied as a pigment to provide whiteness to such products as paints, paper, foodstuffs, medicines, toothpastes, etc. However, neurotropic properties of titanium dioxide remains unclear. This work aimed evaluation of neurotoxic effects of titanium dioxide nanoparticles (12 nm particle size) serially administered to Wistar rats in dose of 250 mg/kg for 7 days. Behavioral and physiological observations were registered immediately after treatment. Results showed that nanoTiO2 particles caused reducing of general motor activity in rats and a shift of the electroencephalogram (EEG) power toward low frequencies of (EEG), while aggressive behavior, and open field behavior did not change. The depressive effect of titanium dioxide nanoparticles on the central nervous system (CNS) observed in our study might be related to neuronal damage caused by an increase in reactive oxygen species (ROS) as well as the impairment of synaptic transmission.

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Experimental induction of steatosis in different tissues after the ingestion of a carbohydrate-rich diet: effect on the liver, on the heart and on indicators of oxidation

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032010000400013 ◽

2010 ◽

Vol 47 (4) ◽

pp. 388-392 ◽

Cited By ~ 3

Author(s):

Nadia Juliana Beraldo Goulart Borges Haubert ◽

Gilberto João Padovan ◽

Sérgio Zucoloto ◽

Hélio Vannucchi ◽

Julio Sergio Marchini

Keyword(s):

Vitamin E ◽

Adult Male ◽

Wistar Rats ◽

Reduced Glutathione ◽

Fat Deposition ◽

Male Wistar Rats ◽

Hepatic Fat ◽

Lipid Vacuoles ◽

Animal House ◽

Experimental Group

CONTEXT: The western dietary pattern is characterized by a high calorie intake with a high proportion of simple sugars. This diet is associated with comorbidities such as hepatic fat deposition and is possibly related to non-alcoholic fatty liver disease. OBJECTIVE: To evaluate the capacity of a hyperglucidic diet to induce steatosis in adult male Wistar rats. After the administration of a carbohydrate-rich diet, we also evaluated the presence of hepatic and cardiac steatosis and the levels of intrinsic antioxidants in the liver. METHODS: Forty-six eutrophic adult male Wistar rats were used and 10 of them were chosen, at random, to serve as controls, while the remaining ones formed the experimental group. Control animals received the standard ration offered by the animal house and the experimental group received the hyperglucidic diet. The diets were offered for 21 days and, at the end of this period, tissue samples were collected for analysis of indicators of oxidative stress (malondialdehyde, and reduced glutathione) and of vitamin E. The animals were then sacrificed by decapitation and their viscera were removed for analysis of liver and heart fat. RESULTS: The hyperglucidic diet used induced hepatic fat deposition, with lipid vacuoles being detected in 83% of the livers analyzed by histology. No lipid vacuoles were observed in the heart. Malondialdehyde and reduced glutathione levels remained unchanged when the animals were submitted to the hyperglucidic diet, probably because there was no liver development of fibrosis or inflammation. In contrast, the levels of vitamin E (antioxidant) were reduced, as confirmed in the literature for steatotic animals. CONCLUSION: The hyperglucidic diet induced hepatic steatosis. In the heart there was an increase in fat content, although no histological changes were observed. These alterations cannot be explained by the presence of malondialdehyde or reduced glutathione (indicators of oxidation), since the values were similar in the groups studied. However, a significant reduction of vitamin E was observed in the experimental group.

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Evaluation of the Efficiency of Combined and Separated Antioxidant Supplementation of Vitamin C and E on Semen Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.7(4).p166-172 ◽

2018 ◽

Vol 7 (4) ◽

pp. 166-172

Author(s):

Amina Benabbou ◽

Meghit Boumediene Khaled ◽

Ali Saeed Alchalabi

Keyword(s):

Diabetes Mellitus ◽

Vitamin E ◽

Vitamin C ◽

Wistar Rats ◽

Sperm Count ◽

Reproductive Capacity ◽

Semen Parameters ◽

Testis Weight ◽

Male Wistar Rats ◽

Hormonal Levels

Male reproductive functions could be affected at multiple levels due to dia-betes mellitus. Antioxidants vitamins have direct impact on improving male reproductive capacity. Our objective was to assess the efficiency of treat-ment with vitamin C and E in the case of whether diabetes mellitus is the cause of infertility or if infertile men suffer from diabetes by inducing diabe-tes on male Wistar rats. Adult male Wistar rats were divided into 5 groups of 6 animals each: a normoglycemic control. Other four groups were given a single dose of streptozotocin, and divided into: diabetic control, diabetic + 250 mg/kg/day of vitamin C, diabetic + 250 mg/kg/day of vitamin E, diabetic + 250 mg/kg/day of vitamin C + 250 mg/kg/day of vitamin E by gavage during 30 consecutive days. Animals were anesthetized, sacrificed to evaluate body and reproductive organ weights, plasma hormone levels, and semen quality. Compared to normoglycemic animals, diabetic rats showed reduced body weight (211.33 ± 8.70gr) and epididymis (1.35 ± 0.10 gr). The testis weight (3.10 ± 0.14 gr) and sperm count (2.72 ± 4.61 x 106) were maintained. A reduction of LH and testosterone levels (0.10 ± 0.00, and 0.25 ± 0.12) respec-tively. There was a significant increase (p<0.05) in the final body and epididy-mis weights, hormonal levels, and sperm count among diabetes vitamins treated groups as compared to the normoglycemic and hyperglycemic groups. Diabetes mellitus induces adverse effects on reproductive capacity. Antioxidant vitamins C and E improves sperm quality and increases hormonal levels.

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