Evaluation of the Efficiency of Combined and Separated Antioxidant Supplementation of Vitamin C and E on Semen Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats

2018 ◽  
Vol 7 (4) ◽  
pp. 166-172
Author(s):  
Amina Benabbou ◽  
Meghit Boumediene Khaled ◽  
Ali Saeed Alchalabi
Keyword(s):  
Diabetes Mellitus ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Sperm Count ◽  
Reproductive Capacity ◽  
Semen Parameters ◽  
Testis Weight ◽  
Male Wistar Rats ◽  
Hormonal Levels

Male reproductive functions could be affected at multiple levels due to dia-betes mellitus. Antioxidants vitamins have direct impact on improving male reproductive capacity. Our objective was to assess the efficiency of treat-ment with vitamin C and E in the case of whether diabetes mellitus is the cause of infertility or if infertile men suffer from diabetes by inducing diabe-tes on male Wistar rats. Adult male Wistar rats were divided into 5 groups of 6 animals each: a normoglycemic control. Other four groups were given a single dose of streptozotocin, and divided into: diabetic control, diabetic + 250 mg/kg/day of vitamin C, diabetic + 250 mg/kg/day of vitamin E, diabetic + 250 mg/kg/day of vitamin C + 250 mg/kg/day of vitamin E by gavage during 30 consecutive days. Animals were anesthetized, sacrificed to evaluate body and reproductive organ weights, plasma hormone levels, and semen quality. Compared to normoglycemic animals, diabetic rats showed reduced body weight (211.33 ± 8.70gr) and epididymis (1.35 ± 0.10 gr). The testis weight (3.10 ± 0.14 gr) and sperm count (2.72 ± 4.61 x 106) were maintained. A reduction of LH and testosterone levels (0.10 ± 0.00, and 0.25 ± 0.12) respec-tively. There was a significant increase (p<0.05) in the final body and epididy-mis weights, hormonal levels, and sperm count among diabetes vitamins treated groups as compared to the normoglycemic and hyperglycemic groups. Diabetes mellitus induces adverse effects on reproductive capacity. Antioxidant vitamins C and E improves sperm quality and increases hormonal levels.

scholarly journals Effectiveness of Vitamin C and Vitamin E Antioxidant Combination on Caspase-3 Expression in Wistar White Rat Pulmonum Contusion

2020 ◽  
Vol 3 (1) ◽  
pp. 31-44
Author(s):  
Bermansyah ◽  
Gama Satria ◽  
Ahmad Umar
Keyword(s):  
Cell Death ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Cell Function ◽  
Pulmonary Contusion ◽  
Tnf Α ◽  
Male Wistar Rats

Introduction.Pulmonary contusions can cause a progressive inflammatory response. Activation of TNF-α cytokines and reactive oxygen species (ROS) can cause pulmonary cell death. Antioxidants can have the potential to neutralize ROS. The purpose of this study is to determine the effectiveness of antioxidant administration in maintaining pulmonary cell function in wistar rats that have been induced to experience pulmonary contusions through caspase-3 levels. Methods.This study was an in vivo experimental study conducted on thirty male wistar rats and divided into five groups (n = 6): control, pulmonary contusion + asthaxanthine 5 mg/kgBW, pulmonary contusion + vitamin C and E 50 mg/kgBW, pulmonary contusion + vitamin C and E 100 mg/kgBW, pulmonary contusion + vitamin C and E 200 mg/kgBW. The value of Caspase-3 is evaluated by the IHC. All data analyzes used SPSS 18. Results. Low doses of antioxidants have the potential to reduce pulmonary cell death in wistar rats induced by pulmonary contusions.Conclussion. Vitamin C and E effective to reduce polmonary cell death in pulmonary contusion.Keywords: antioxidants, vitamin C, vitamin E, pulmonary contusions animal model, apoptosis, caspase-3

scholarly journals Melatonin and Vitamin C Attenuate Cassava Diet-Induced Alteration in Thyroid Function

2020 ◽  
Author(s):  
Abdullateef Alagbonsi ◽  
Oloruntobi Oluwasegun Maliki ◽  
Comfort Moyinolowa Ibitoye ◽  
Luqman Aribidesi Olayaki
Keyword(s):  
Body Weight ◽  
Vitamin C ◽  
Thyroid Function ◽  
Sperm Count ◽  
Thyroid Stimulating Hormone ◽  
Semen Parameters ◽  
Saline Control ◽  
Treatment Groups ◽  
Total Antioxidant ◽  
Male Wistar Rats

Abstract Background Cyanide is present in cassava and is well known to cause adverse effects on the male reproductive functions. This study evaluated the effect of melatonin and/or vitamin C on body weight, thyroid function and reproductive parameters in male Wistar rats treated with cyanide-enriched cassava-diet (CD), and their possible mechanisms of actions. Methods Forty-five (45) animals were divided into 9 groups (n = 5 each) that received the following treatments for 28 days. Groups I-III received normal saline (control), melatonin (15 mg/kg), and vitamin C (100 mg/kg) only. Groups IV-VI received 40% CD, while groups V and VI were additionally treated with melatonin only and melatonin and vitamin C respectively. Groups VII-IX received 80% CD, but groups VIII and IX were additionally treated with melatonin only and melatonin and vitamin C respectively. Results Melatonin and/or vitamin C supplement increased body weight in CD-treated rats. The sperm count (but not other semen parameters) was increased by CD and melatonin, while combination of melatonin and vitamin C in CD-treated rats increased all semen parameters. Neither CD alone nor its co-administration with melatonin and /or vitamin C affected plasma luteinising hormone (LH) and testosterone. The 40% CD and 80% CD increased triiodothyronine (T3), but the increase by the former was abolished by melatonin alone while the increase by the latter was neither affected by melatonin alone nor its combination with vitamin C. Moreover, the 40% CD and 80% CD increased thyroxine (T4), but was neither affected by melatonin alone or its combination with vitamin C. The levels of thyroid stimulating hormone (TSH) were not different across all treatment groups. Both 40% CD and 80% CD increased the thiocyanate level, which was ameliorated by melatonin but abolished by combination of melatonin and vitamin C. Both 40% CD and 80% CD decreased the total antioxidant capacity (TAC) level, which was abolished by melatonin. Conclusion In conclusion, this study suggests that CD increases weight gain, thyroid hormone and oxidative stress, which were attenuated by anti-oxidants melatonin and vitamin C.

scholarly journals PERBEDAAN ANTARA EFEK PEMBERIAN VITAMIN C DAN VITAMIN E TERHADAP KUALITAS SPERMATOZOA TIKUS WISTAR (RATTUS NORVEGICUS) JANTAN SETELAH DIBERI PAPARAN ASAP ROKOK

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Astrid Giovani Sitohang ◽  
Benny Wantouw ◽  
Edwin De Queljoe
Keyword(s):  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Water Soluble ◽  
Male Wistar Rats ◽  
The Mean

Abstract: Gaseous components of cigarette smoke have a potential to generate free radicals that can damage sperm. Vitamin C is water soluble and vitamin E is a fat-soluble antioxidant that is effective in dealing with free radicals. The aim of this research was to look at the differences between the effects of vitamin C and vitamin E on spermatozoa quality of male wistar rats (Rattus norvegicus) after exposure to cigarette smoke. Methods: This research used a completely randomized experimental design. The sample of the research were 15 male wistar rats which were randomly divided into 3 groups, i.e. group P0 was given exposure to smoke from 2 bars of cigarette without giving vitamin C and vitamin E, group P1 was given exposure to smoke from 2 bars of cigarette and vitamin C 1,8 mg / day and group P2 was given exposure to smoke from 2 bars of cigarette and vitamin E 1,44 mg / day. The treatment was given for 52 days. Results: The results of this research showed an increasing spermatozoa quality of group P1 and P2 compared with the group P0. The mean concentration of spermatozoa obtained on group P1 (78,78x106/ml) and group P2 (123,13 x106/ml). The mean motility of spermatozoa obtained on group P1 (72,00%) and group P2 (80,25%). The mean of normal morphology of spermatozoa obtained on group P1 (67,63%) and group P2 (74,00%). Conclusion: The conclusion from this research showed that there is a difference between spermatozoa quality of group treated with vitamin C and vitamin E after exposure to cigarette smoke, with vitamin E had an higher average on spermatozoa quality.Keywords: cigarette smoke, vitamin C, vitamin E, the quality of spermatozoaAbstrak: Latar Belakang: Komponen gas dari asap rokok berpotensi untuk menimbulkan radikal bebas yang dapat merusak spermatozoa. Vitamin C yang larut dalam air dan vitamin E yang larut dalam lemak merupakan antioksidan yang efektif dalam mengatasi radikal bebas. Tujuan penelitian ini adalah untuk melihat perbedaan efek antara pemberian vitamin C dan vitamin E terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) jantan setelah diberi paparan asap rokok. Metode: Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak lengkap. Sampel yang digunakan adalah 15 tikus wistar jantan yang dibagi secara acak menjadi 3 kelompok yaitu kelompok P0 yang mendapat perlakuan paparan asap rokok 2 batang tanpa pemberian vitamin C dan vitamin E, kelompok P1 dengan perlakuan pemaparan asap rokok 2 batang dan vitamin C 1,8 mg/hari dan kelompok P2 dengan perlakuan pemaparan asap rokok 2 batang dan vitamin E 1,44 mg/hari. Perlakuan diberikan selama 52 hari. Hasil: Hasil penelitian menunjukkan adanya peningkatan kualitas spermatozoa kelompok P1 dan P2 dibandingkan dengan kelompok P0. Rata-rata konsentrasi spermatozoa kelompok P1 didapatkan (78,78x106/ml) dan kelompok P2 (123,13 x106/ml). Rata-rata motilitas spermatozoa kelompok P1 (72,00%) dan kelompok P2 (80,25%). Rata-rata morfologi normal spermatozoa kelompok P1 (67,63%) dan kelompok P2 (74,00%). Simpulan: Kesimpulan dari penelitian ini didapatkan bahwa terjadi perbedaan kualitas spermatozoa antara pemberian vitamin C dan vitamin E setelah pemaparan asap rokok, dengan vitamin E memiliki rata-rata kualitas spermatozoa lebih tinggi.Kata kunci: asap rokok, vitamin C, vitamin E, kualitas spermatozoa

Effect of vitamin A and vitamin C on attenuation of ivermectin-induced toxicity in male Wistar rats

2018 ◽  
Vol 25 (29) ◽  
pp. 29408-29417 ◽  
Author(s):  
Fatemeh Sadat Hosseini Omshi ◽  
Roghayeh Abbasalipourkabir ◽  
Maliheh Abbasalipourkabir ◽  
Sedigheh Nabyan ◽  
Alireza Bashiri ◽  
...  
Keyword(s):  
Vitamin A ◽  
Vitamin C ◽  
Wistar Rats ◽  
Male Wistar Rats

scholarly journals Increase of collagen in diabetes-related traumatic ulcers after the application of liquid smoke coconut shell

2017 ◽  
Vol 50 (2) ◽  
pp. 71
Author(s):  
Meircurius Dwi Condro Surboyo ◽  
Ira Arundina ◽  
Retno Pudji Rahayu
Keyword(s):  
Diabetes Mellitus ◽  
Topical Application ◽  
Wistar Rats ◽  
Healing Process ◽  
Coconut Shell ◽  
Diabetic Patients ◽  
Process Indicator ◽  
Liquid Smoke ◽  
Patients With Diabetes ◽  
Male Wistar Rats

Background: Traumatic ulcers in patients with diabetes mellitus may experience delayed healing despite their diabetic condition being controlled. Liquid smoke coconut shell containing phenolic compounds can potentially accelerate the healing process. One healing process indicator is the increased number of fibroblasts, another being the increased amount of collagen. Purpose: This study aimed to analyze the amount of collagen in traumatic ulcers in diabetics after application of liquid smoke coconut shell. Methods: Alloxan was induced in twenty-four male Wistar rats as models of diabetes mellitus. A traumatic 10 mm ulcer was made along the labial fornix incisive inferior with a round, stainless steel blade before liquid smoke coconut shell and benzydamine hydrochloride (as the control) was administrated once a day. A biopsy of the labial fornix incisive inferior was subsequently performed after the topical application for 5 and 7 days. Histological assessment was conducted to analyze the amount of collagen by means of Masson Trichome staining. Results: Histologically, the topical application of liquid smoke coconut shell for 5 days significantly increased the amount of collagen, higher than that of benzydamine hydrochloride as the control (p=0.006) (p<0.05). Meanwhile, the topical application of liquid smoke coconut shell for 7 days made the concentration of collagen no significantly different from that of benzydamine hydrochloride as the control (p=0.156) (p>0.05). Conclusion: Liquid smoke coconut shell applied for 5 days increase the amount of collagen in traumatic ulcers in diabetic patients.

scholarly journals Pengaruh Pemberian Antioksidan Vitamin C Dan E Terhadap Konsentrasi Spermatozoa Mencit (Mus Musculus) Yang Dipapar Asap Rokok

2017 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Ervina Wati Harahap ◽  
Normalina Sandora ◽  
Winarto Winarto
Keyword(s):  
Reactive Oxygen Species ◽  
Free Radical ◽  
Vitamin E ◽  
Vitamin C ◽  
Male Infertility ◽  
Research Design ◽  
Mus Musculus ◽  
Sperm Count ◽  
Control Group ◽  
Oxygen Species

Free radical that are released in cigarette smoke, believed to play an important role in male infertility. The free radicalproduces Reactive Oxygen Species (ROS) that is found increased in male smokers. ROS is one of the free radicalscomponent can be neutralized by vitamin C and E as an antioxidant. This study using those agents to the fifteen minuteshalf piece cigarette to expose mice and count their sperm as the fertilility parameter. The research design used was posttest-only control group with twenty mice were divided into five groups. A group of no smoke exposed was categorizedas a baseline control, the exposed groups were consisted of one group without antioxidant, one group with vitamin Ctreatment, a group of vitamin E treatment and at last the combination of vitamin C and E. All groups were treatedfor fourteen days. The results were analyzed using oneway ANOVA with p<0.05. It is obviously proven that thecombination of vitamin C and vitamin E treatment on the usual dose gave the highest sperm count compared to othergroups.

Sign in / Sign up

Export Citation Format

Share Document