Selection of suitable reference genes for core clock gene expression analysis by real-time qPCR in rat ovary granulosa cells

2019 ◽  
Vol 46 (3) ◽  
pp. 2941-2946 ◽  
Author(s):  
Chuanjiang Cai ◽  
Pengpeng Cai ◽  
Guiyan Chu
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Gene Expression Analysis ◽  
Clock Gene ◽  
Clock Gene Expression ◽  
Rat Ovary ◽  
Suitable Reference ◽  
Core Clock Gene ◽  
Real Time Qpcr ◽  
Selection Of

Hormonal regulation of core clock gene expression in skeletal muscle following acute aerobic exercise

2019 ◽  
Vol 508 (3) ◽  
pp. 871-876 ◽  
Author(s):  
Patrick G. Saracino ◽  
Michael L. Rossetti ◽  
Jennifer L. Steiner ◽  
Bradley S. Gordon
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Aerobic Exercise ◽  
Hormonal Regulation ◽  
Clock Gene ◽  
Clock Gene Expression ◽  
Core Clock Gene

scholarly journals Time-restricted feeding alters lipid and amino acid metabolite rhythmicity without perturbing clock gene expression

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Leonidas S. Lundell ◽  
Evelyn B. Parr ◽  
Brooke L. Devlin ◽  
Lars R. Ingerslev ◽  
Ali Altıntaş ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Serum Lipids ◽  
Clock Gene ◽  
Energy Restriction ◽  
Restricted Feeding ◽  
Short Term ◽  
Macronutrient Composition ◽  
Clock Gene Expression ◽  
Core Clock Gene

Abstract Time-restricted feeding (TRF) improves metabolism independent of dietary macronutrient composition or energy restriction. To elucidate mechanisms underpinning the effects of short-term TRF, we investigated skeletal muscle and serum metabolic and transcriptomic profiles from 11 men with overweight/obesity after TRF (8 h day−1) and extended feeding (EXF, 15 h day−1) in a randomised cross-over design (trial registration: ACTRN12617000165381). Here we show that muscle core clock gene expression was similar after both interventions. TRF increases the amplitude of oscillating muscle transcripts, but not muscle or serum metabolites. In muscle, TRF induces rhythmicity of several amino acid transporter genes and metabolites. In serum, lipids are the largest class of periodic metabolites, while the majority of phase-shifted metabolites are amino acid related. In conclusion, short-term TRF in overweight men affects the rhythmicity of serum and muscle metabolites and regulates the rhythmicity of genes controlling amino acid transport, without perturbing core clock gene expression.

scholarly journals MicroRNAs modulate core-clock gene expression in pancreatic islets during early postnatal life in rats

Diabetologia ◽  
2017 ◽  
Vol 60 (10) ◽  
pp. 2011-2020 ◽  
Author(s):  
Cécile Jacovetti ◽  
Adriana Rodriguez-Trejo ◽  
Claudiane Guay ◽  
Jonathan Sobel ◽  
Sonia Gattesco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pancreatic Islets ◽  
Clock Gene ◽  
Postnatal Life ◽  
Clock Gene Expression ◽  
Early Postnatal Life ◽  
Core Clock Gene

scholarly journals Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yunxing Zhang ◽  
Xiaojiao Han ◽  
Shuangshuang Chen ◽  
Liu Zheng ◽  
Xuelian He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Abiotic Stresses ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Quantitative Real Time Pcr ◽  
Salix Matsudana ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Diurnal variations in the expression of core-clock genes correlate with resting muscle properties and predict fluctuations in exercise performance across the day

2021 ◽  
Vol 7 (1) ◽  
pp. e000876
Author(s):  
Alireza Basti ◽  
Müge Yalçin ◽  
David Herms ◽  
Janina Hesse ◽  
Ouda Aboumanify ◽  
...  
Keyword(s):  
Gene Expression ◽  
Exercise Performance ◽  
Clock Gene ◽  
Clock Genes ◽  
Muscle Tone ◽  
Muscle Properties ◽  
Clock Gene Expression ◽  
Digital Palpation ◽  
Resting Muscle ◽  
Core Clock Gene

ObjectivesIn this study, we investigated daily fluctuations in molecular (gene expression) and physiological (biomechanical muscle properties) features in human peripheral cells and their correlation with exercise performance.Methods21 healthy participants (13 men and 8 women) took part in three test series: for the molecular analysis, 15 participants provided hair, blood or saliva time-course sampling for the rhythmicity analysis of core-clock gene expression via RT-PCR. For the exercise tests, 16 participants conducted strength and endurance exercises at different times of the day (9h, 12h, 15h and 18h). Myotonometry was carried out using a digital palpation device (MyotonPRO), five muscles were measured in 11 participants. A computational analysis was performed to relate core-clock gene expression, resting muscle tone and exercise performance.ResultsCore-clock genes show daily fluctuations in expression in all biological samples tested for all participants. Exercise performance peaks in the late afternoon (15–18 hours for both men and women) and shows variations in performance, depending on the type of exercise (eg, strength vs endurance). Muscle tone varies across the day and higher muscle tone correlates with better performance. Molecular daily profiles correlate with daily variation in exercise performance.ConclusionTraining programmes can profit from these findings to increase efficiency and fine-tune timing of training sessions based on the individual molecular data. Our results can benefit both professional athletes, where a fraction of seconds may allow for a gold medal, and rehabilitation in clinical settings to increase therapy efficacy and reduce recovery times.

scholarly journals Conserved and Divergent Rhythms of Crassulacean Acid Metabolism-Related and Core Clock Gene Expression in the Cactus Opuntia ficus-indica

2011 ◽  
Vol 156 (4) ◽  
pp. 1978-1989 ◽  
Author(s):  
Izaskun Mallona ◽  
Marcos Egea-Cortines ◽  
Julia Weiss
Keyword(s):  
Gene Expression ◽  
Crassulacean Acid Metabolism ◽  
Clock Gene ◽  
Acid Metabolism ◽  
Opuntia Ficus Indica ◽  
Clock Gene Expression ◽  
Core Clock Gene

Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Mulberry (Morus alba L.) under different abiotic stresses

2019 ◽  
Vol 46 (2) ◽  
pp. 1809-1817 ◽  
Author(s):  
Pawan Shukla ◽  
Ramesha A. Reddy ◽  
Kangayam M. Ponnuvel ◽  
Gulab Khan Rohela ◽  
Aftab A. Shabnam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Analysis ◽  
Real Time Pcr ◽  
Abiotic Stresses ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Quantitative Real Time Pcr ◽  
Suitable Reference ◽  
Selection Of

scholarly journals Interaction of ovarian steroidogenesis and clock gene expression modulated by bone morphogenetic protein-7 in human granulosa cells

2019 ◽  
Vol 66 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Satoko Nagao ◽  
Nahoko Iwata ◽  
Yoshiaki Soejima ◽  
Takaaki Takiguchi ◽  
Tamami Aokage ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Granulosa Cells ◽  
Clock Gene ◽  
Bone Morphogenetic Protein 7 ◽  
Ovarian Steroidogenesis ◽  
Human Granulosa Cells ◽  
Clock Gene Expression ◽  
Morphogenetic Protein
Sign in / Sign up

Export Citation Format

Share Document