The role of SIRT5 and p53 proteins in the sensitivity of colon cancer cells to chemotherapeutic agent 5-Fluorouracil

2021 ◽  
Author(s):  
Ozlem Ekremoglu ◽  
Asli Koc
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Chemotherapeutic Agent ◽  
Colon Cancer Cells

scholarly journals The Role of SIRT5 and p53 Proteins in the Sensitivity of Colon Cancer Cells to Chemotherapeutic Agent 5-Fluorouracil 

2021 ◽  
Author(s):  
Ozlem Ekremoglu ◽  
Asli Koc
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Chemotherapeutic Agent ◽  
P53 Protein ◽  
Colon Cancer Cells ◽  
Independent Manner ◽  
Protein Expressions ◽  
Western Blot Experiment ◽  
Hct 116

Abstract In the treatment of colorectal cancer, it is important to develop drug combinations that will increase the effectiveness of chemotherapy and to determine the molecular targets of the drugs. Therefore, combined therapies that can increase the sensitivity of 5-Fluourouracil (5-FU), a chemotherapeutic agent used in the treatment of colon cancer, and also the molecular pathways involved in this process are important in the treatment of the disease. Here we examined the SIRT5 (Resveratrol and Suramin) and p53 (Nutlin3a) modulators alone or in combination with 5-FU on the proliferation of colon cancer cells and effect of 5-FU on the SIRT5 and FOXO3a expressions whether p53 dependent or independent manner. p53 protein expression is activated by nutlin3a in HCT-116 p53 +/+ cells and HCT-116 p53 -/- cells were used as counterpart cells that did not express p53. According to our MTT assay results, we showed that these modulators have significant effects on the sensitivity of both cells to 5-FU. In p53+/+ cells, Resveratrol (RSV) and Nutlin3a and in p53 -/- cells, Suramin was found to be more effective. These differences were evaluated together with the effect of 5-FU on the SIRT5-FOXO3a-Bim axis in p53 +/+ and p53 -/- cells. SIRT5 is known to deactivate FOXO3a which plays a role in the induction of apoptosis via Bim. Our western blot experiment results showed that while Suramin decreased SIRT5 and RSV decreased FOXO3a protein expressions significantly in HCT-116 p53 -/- cells, 5-FU decreased significantly SIRT5 and FOXO3a protein expressions p53 independently. These results may help the discovery of new markers in colon cancer treatment.

Role of SPARC in the epithelial mesenchymal transition induced by PTHrP in human colon cancer cells

2021 ◽  
pp. 111253
Author(s):  
Pedro Carriere ◽  
Natalia Calvo ◽  
María Belén Novoa ◽  
Fernanda Lopez-Moncada ◽  
Alexander Riquelme ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Epithelial Mesenchymal Transition ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Mesenchymal Transition ◽  
Human Colon Cancer Cells

scholarly journals Exosomes secreted from human colon cancer cells influence the adhesion of neighboring metastatic cells: Role of microRNA-210

2016 ◽  
Vol 17 (10) ◽  
pp. 1062-1069 ◽  
Author(s):  
Elisabetta Bigagli ◽  
Cristina Luceri ◽  
Daniele Guasti ◽  
Lorenzo Cinci
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Metastatic Cells ◽  
Human Colon Cancer Cells

Confluence-dependent resistance in human colon cancer cells: Role of reduced drug accumulation and low intrinsic chemosensitivity of resting cells

1992 ◽  
Vol 50 (5) ◽  
pp. 677-682 ◽  
Author(s):  
Marie-ThéRéSe Dimanche-Boitrel ◽  
Hélène Pelletier ◽  
Philippe Genne ◽  
Jean-Michel Petit ◽  
Christian Le Grimellec ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Drug Accumulation ◽  
Resting Cells ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

Role of Dietary Calcium in Growth Modulation of Human Colon Cancer Cells

2000 ◽  
pp. 324-326 ◽  
Author(s):  
E. Kállay ◽  
E. Bajna ◽  
S. Kriwanek ◽  
E. Bonner ◽  
M. Peterlik ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Dietary Calcium ◽  
Colon Cancer Cells ◽  
Growth Modulation ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

scholarly journals Role of pannexin-1 in the cellular uptake, release and hydrolysis of anandamide by T84 colon cancer cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mireille Alhouayek ◽  
René Sorti ◽  
Jonathan D. Gilthorpe ◽  
Christopher J. Fowler
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Colon Cancer Cells ◽  
Pannexin 1 ◽  
Hydrolysis Of

Role of AXL in invasion and drug resistance of breast and colon cancer cells

2016 ◽  
Vol 61 ◽  
pp. S79
Author(s):  
W.M. Abdel-Rahman ◽  
N.A. Al-khayyal ◽  
V.A. Nair ◽  
M.S. Ayad
Keyword(s):  
Colon Cancer ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Colon Cancer Cells
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