Candida Colonization in Urine Samples of ICU Patients: Determination of Etiology, Antifungal Susceptibility Testing and Evaluation of Associated Risk Factors

2012 ◽  
Vol 174 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Nidhi Singla ◽  
Neelam Gulati ◽  
Neelam Kaistha ◽  
Jagdish Chander
Keyword(s):  
Risk Factors ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Urine Samples ◽  
Candida Colonization ◽  
Icu Patients ◽  
Associated Risk Factors ◽  
Testing And Evaluation

scholarly journals Candida Isolates From Blood and Other Normally Sterile Foci From ICU Patients: Determination of Epidemiology, Antifungal Susceptibility Profile and Evaluation of Associated Risk Factors

2021 ◽  
Vol 9 ◽  
Author(s):  
Bo Wang ◽  
Xinlong He ◽  
Feng Lu ◽  
Yajuan Li ◽  
Yuerong Wang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Molecular Mechanisms ◽  
Antifungal Susceptibility ◽  
Resistance Mechanism ◽  
Lavage Fluid ◽  
Icu Patients ◽  
Multiple Risk Factors ◽  
Microdilution Method ◽  
Associated Risk Factors

Background: The clinical diagnosis and therapy for ICU patients with invasive candidiasis are challenged by the changes of Candida community composition and antimicrobial resistance. The epidemiology and drug sensitivity of candidiasis in ICU as well as its risk factors and drug resistance mechanism were investigated.Methods: In the present study, 115 patients in ICU were recruited from June 2019 through July 2020. Among them, 83 Candida isolates were identified with MALDI-TOF mass spectrometry. The susceptibility to antifungals was measured by microdilution method. The molecular mechanisms of azole-resistant Candida tropicalis were explored by sequencing, and their outcomes were explicitly documented.Results:Candida glabrata and C. tropicalis were the predominant non-C. albicans Candida. The specimen sources were mainly urine, bronchoalveolar lavage fluid and blood. The age, length of hospitalization, tracheotomy, diabetes and concomitant bacterial infection were the main risk factors for candidiasis. The majority of Candida species exhibited susceptibility to antifungals. However, certain C. tropicalis were frequently resistant to azoles. The polymorphism of the ERG11 in C. tropicalis was likely associated with azole resistance.Conclusion: The multiple risk factors for candidiasis in ICU patients need to be considered. Certain C. tropicalis exhibit resistance to azoles likely due to the ERG11 gene polymorphism.

scholarly journals Multicenter Comparison of the Etest and EUCAST Methods for Antifungal Susceptibility Testing of Candida Isolates to Micafungin

2016 ◽  
Vol 60 (8) ◽  
pp. 5088-5091 ◽  
Author(s):  
M.-E. Bougnoux ◽  
E. Dannaoui ◽  
I. Accoceberry ◽  
A. Angoulvant ◽  
E. Bailly ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Antifungal Susceptibility Testing ◽  
Medical Mycology ◽  
Single Center ◽  
Content Type ◽  
Valuable Alternative

ABSTRACTIn vitrosusceptibility of 933Candidaisolates, from 16 French hospitals, to micafungin was determined using the Etest in each center. All isolates were then sent to a single center for determination of MICs by the EUCAST reference method. Overall essential agreement between the two tests was 98.5% at ±2 log2dilutions and 90.2% at ±1 log2dilutions. Categorical agreement was 98.2%. The Etest is a valuable alternative to EUCAST for the routine determination of micafungin MICs in medical mycology laboratories.

scholarly journals Epidemiology of urinary candidiasis and antifungal susceptibility pattern of various Candida species at a rural tertiary health care centre of Puducherry, South India- An observational study

2021 ◽  
Vol 7 (2) ◽  
pp. 103-107
Author(s):  
Udhaya Sankar Ranganathan ◽  
Nagma Rafi ◽  
Gopal Rangasamy ◽  
Mangaiyarkarasi Thiyagarajan ◽  
Sunil Shivekar
Keyword(s):  
Risk Factors ◽  
Observational Study ◽  
Amphotericin B ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Diffusion Method ◽  
Urinary Catheterization ◽  
Resistance Patterns ◽  
Phenotypic Methods

Urinary tract infection (UTI) is a common health problem across the globe. Although majority of them are bacterial in origin, there has been an increasing trend in the incidence of UTI due to the yeast like fungi, . UTI due to are in large proportion noscomially acquired and of growing concern is the development of resistance to the commonly used azole group of drugs for their treatment. Since the resistance is more commonly reported among non- albicans , routine species identification and antifungal susceptibility testing is crucial for successful clinical outcomes. This study was conducted to analyse the distribution and risk factors associated with Urinary candidiasis and also to determine the resistance patterns of different to various antifungal agents using phenotypic methods. : A hospital based observational study was conducted from September 2016 to December 2017 on patients presenting with symptomatic UTI. isolates were speciated using phenotypic methods like germ tube test and growth character on chromagar . Antifungal susceptibility to fluconazole, voriconazole, ketoconazole and amphotericin B were determined using disc diffusion method. All data were anlyzed using EpiData Analysis software version 2.2.2.186. A total of 101 were isolated. The incidence of Urinary candidiasis was more among females (66.4%) than in males (33.6%). People above 50 years (38.6%) were commonly affected followed by people in the age group of 21-30 years (22.7%). The incidence among hospitalized patients was 86.7% and urinary catheterization (43.5%) was the most commonly associated risk factor. The most common isolates were tropicalis (31.6%) followed by albicans (21.7%). The overall resistance patterns among various were 50.5%, 32.7%, 19.9% and 2% for fluconazole, ketoconazole, voriconazole and amphotericin B respectively. Hospitalization and urinary catheterization are the important risk factors for developing urinary candidiasis. The antifungal susceptibility varies among different and hence, identification of to species level along with antifungal susceptibility testing should be practiced as a routine in all clinical mycology laboratories.

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