TALEN-mediated targeted mutagenesis of fatty acid desaturase 2 (FAD2) in peanut (Arachis hypogaea L.) promotes the accumulation of oleic acid

2018 ◽  
Vol 97 (1-2) ◽  
pp. 177-185 ◽  
Author(s):  
Shijie Wen ◽  
Hao Liu ◽  
Xingyu Li ◽  
Xiaoping Chen ◽  
Yanbin Hong ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Arachis Hypogaea ◽  
Fatty Acid Desaturase ◽  
Targeted Mutagenesis ◽  
Arachis Hypogaea L ◽  
Fatty Acid Desaturase 2

Isolation and characterization of fatty acid desaturase genes from peanut (Arachis hypogaea L.)

2011 ◽  
Vol 30 (8) ◽  
pp. 1393-1404 ◽  
Author(s):  
Xiaoyuan Chi ◽  
Qingli Yang ◽  
Lijuan Pan ◽  
Mingna Chen ◽  
Yanan He ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Arachis Hypogaea ◽  
Fatty Acid Desaturase ◽  
Isolation And Characterization ◽  
Arachis Hypogaea L

scholarly journals Lipid, Protein, and Ash Contents, and Fatty Acid and Sterol Compositions of Peanut (Arachis hypogaea L.) Seeds from Ecuador

1995 ◽  
Vol 22 (2) ◽  
pp. 84-89 ◽  
Author(s):  
Nelson R. Grosso ◽  
Carlos A. Guzmán
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Arachis Hypogaea ◽  
Iodine Value ◽  
Sterol Composition ◽  
Arachis Hypogaea L ◽  
Lower Protein

Abstract Oil and protein percentages, ash, iodine value, fatty acid and sterol compositions were studied in 28 Arachis hypogaea L. cultivars originating from Ecuador. Results showed lower protein percentages in the varieties hypogaea (27.3%) and hirsuta (25.9%) than in the varieties fastigiata (29.4%), peruviana (29.4%), and aequatoriana (31.3%). The principal fatty acids were linoleic and oleic. The variety hypogaea exhibited higher concentrations of oleic acid (45.1%). The sterol composition showed higher concentration of β-sitosterol following by campesterol, stigmasterol, and Δ5-avenasterol.

scholarly journals Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

2014 ◽  
Vol 12 (7) ◽  
pp. 934-940 ◽  
Author(s):  
William Haun ◽  
Andrew Coffman ◽  
Benjamin M. Clasen ◽  
Zachary L. Demorest ◽  
Anita Lowy ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Gene Family ◽  
Oil Quality ◽  
Fatty Acid Desaturase ◽  
Targeted Mutagenesis ◽  
Fatty Acid Desaturase 2

scholarly journals Isolation and functional analysis of fatty acid desaturase genes from peanut (Arachis hypogaea L.)

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0189759 ◽  
Author(s):  
Xiaoyuan Chi ◽  
Zhimeng Zhang ◽  
Na Chen ◽  
Xiaowen Zhang ◽  
Mian Wang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Fatty Acid ◽  
Arachis Hypogaea ◽  
Fatty Acid Desaturase ◽  
Arachis Hypogaea L

scholarly journals Genotyping and Fatty Acid Composition Analysis in Segregating Peanut (Arachis hypogaea L.) Populations

2011 ◽  
Vol 38 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Noelle A. Barkley ◽  
Kelly D. Chenault Chamberlin ◽  
Ming Li Wang ◽  
Roy N. Pittman
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Arachis Hypogaea ◽  
Acid Composition ◽  
Total Fatty Acid ◽  
Seed Quality ◽  
Composition Analysis ◽  
Total Fatty Acid Composition ◽  
Arachis Hypogaea L

Abstract Oleic acid (C18∶1), a monounsaturated omega-9 fatty acid, is an important seed quality trait in peanut (Arachis hypogaea L.) because it provides enhanced fatty acid composition, a beneficial effect on human health, improved flavor, and increased shelf life for stored food products by delaying rancidity. Consequently, an emphasis has been placed on breeding peanuts with high levels of oleic acid and low levels of linoleic acid (C18∶2), a polyunsaturated, omega-6 fatty acid. Therefore, crosses were prepared between high oleic and normal peanut lines to develop segregating F2 populations. Total fatty acid composition and the ahFAD2B genotype were determined in the parents and progeny. The oleic to linoleic (O/L) ratio varied from 0.85 to 30.30 in the F2 progeny. Comparing the mean oleic acid values from the three genotypic classes (Ol2Ol2, Ol2ol2, and ol2ol2) in each population confirmed that the means were significantly different. Statistical analysis demonstrated that oleic acid was negatively correlated with linoleic (C18∶2) and palmitic acid (C16∶0), but was positively correlated with gadoleic (C20∶1) and lignoceric (C24∶0) fatty acids. This suggests that modifier genes may influence fatty acid composition. Principally, integration of genotyping and phenotyping data from segregating populations provided valuable insights on the genetic factors controlling total fatty acid composition.

scholarly journals C-to-G Base Editing Enhances Oleic Acid Production by Generating Novel Alleles of FATTY ACID DESATURASE 2 in Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Mid-Eum Park ◽  
Jae-Young Yun ◽  
Hyun Uk Kim
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Vegetable Oil ◽  
Agronomic Traits ◽  
Fatty Acid Desaturase ◽  
Null Alleles ◽  
In Planta ◽  
Growth Defects ◽  
Base Substitutions ◽  
Fatty Acid Desaturase 2

The demand for vegetable oil, which is mainly used for dietary purposes and cooking, is steadily increasing worldwide. It is often desirable to reduce unsaturation levels of fatty acids in order to increase storage stability and reduce trans-fat generation during cooking. Functional disruption of FATTY ACID DESATURASE 2 (FAD2) prevents the conversion of monounsaturated oleic acid to polyunsaturated linoleic acid, thereby enhancing the production of the desirable oleic acid. However, FAD2 null alleles, due to growth defects under stress conditions, are impractical for agronomical purposes. Here, we aimed to attenuate FAD2 activity in planta while avoiding adverse growth effects by introducing amino-acid substitutions using CRISPR base editors. In Arabidopsis, we applied the adenine base editor (ABE) and cytosine base editor (CBE) to induce semi-random base substitutions within several selected FAD2 coding regions. Isolation of base-edited fad2 alleles with higher oleic acid revealed that the CBE application induced C-to-T and/or C-to-G base substitutions within the targeted sequences, resulting in an alteration of the FAD2 enzyme activities; for example, fad2-144 with multiple C-to-G base substitutions showed less growth defects but with a significant increase in oleic acids by 3-fold higher than wild type. Our “proof-of-concept” approach suggests that equivalent alleles may be generated in vegetable oil crops via precision genome editing for practical cultivation. Our targeted semi-random strategy may serve as a new complementary platform for planta engineering of useful agronomic traits.

Sign in / Sign up

Export Citation Format

Share Document