Antifilarial activity of ethyl acetate extract of Vitex negundo leaves in vitro

The current study was aimed to investigate the antifilarial activity of aqueous and ethyl acetate extracts of the leaves of Lannea coromandelica. Both extracts were screened for their total flavonoid content chromatographically. The total amount of flavonoid present in ethyl acetate is much greater than in aqueous extract. Aqueous extract produced initial stimulation effect in whole worm followed by reversible paralysis, whereas on the nerve muscle preparation, the effect was depressant. In the same manner ethyl acetate extract produces irreversible paralysis. The concentrations required to inhibit the movement of the whole worm and nerve muscle preparation for aqueous extract of leaves of L. coromandelica were 300 and 60 µg/mL, respectively, whereas ethyl acetate extract caused inhibition of the whole worm and nerve muscle preparation at 160 and 15 µg/mL, respectively. LC50 and LC90 were 21 and 30 ng/mL, respectively for aqueous, which were 16 and 24 ng/mL for ethyl acetate extracts.

Download Full-text

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

Current Drug Discovery Technologies ◽

10.2174/1570163817666200727101300 ◽

2020 ◽

Vol 17 ◽

Author(s):

Shubhaisi Das ◽

Sunanda Burman ◽

Goutam Chandra

Keyword(s):

Ethyl Acetate ◽

Bioactive Compounds ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Positive Control ◽

Leaf Extracts ◽

Ms Analysis ◽

Ft Ir ◽

Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

Download Full-text

Chemical constituents, in vitro antioxidant and antimicrobial properties of ethyl acetate extract obtained from Cytisus triflorus l’Her

Natural Product Research ◽

10.1080/14786419.2018.1519816 ◽

2018 ◽

Vol 34 (11) ◽

pp. 1586-1590 ◽

Cited By ~ 2

Author(s):

Wassila Benabderrahmane ◽

Amel Amrani ◽

Ouahiba Benaissa ◽

Marta Lores ◽

J. Pablo Lamas ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Constituents ◽

Antimicrobial Properties

Download Full-text

Effect of Eucalyptus globulus leaves extracts on in vitro rumen fermentation, methanogenesis, degradability and protozoa population

Annals of Animal Science ◽

10.2478/aoas-2018-0006 ◽

2018 ◽

Vol 18 (3) ◽

pp. 753-767 ◽

Cited By ~ 4

Author(s):

Amina Boussaada ◽

Rabah Arhab ◽

Serena Calabrò ◽

Raffaella Grazioli ◽

Maria Ferrara ◽

...

Keyword(s):

Organic Matter ◽

Ethyl Acetate ◽

Eucalyptus Globulus ◽

Ethyl Acetate Extract ◽

Rumen Fermentation ◽

Gas Production ◽

Aqueous Extracts ◽

Ch4 Production ◽

In Vitro Gas Production

Abstract The aim of the research was to evaluate the effect of three Eucalyptus globulus extracts rich in phenolic compounds, especially flavonoids, on rumen fermentation, methane (CH4) production, organic matter degradability and protozoa population using an in vitro gas production technique. Four concentrations (0, 50, 75 and 100 mg) of three Eucalyptus extracts (ethyl acetate, n-butanol and aqueous) were added to a diet of ruminants (forage: concentrate ratio 60:40) and incubated at 39°C under anaerobiosis with buffered rumen fluid. After 24 h, the fermentation fluid was analysed for ammonia-N and volatile fatty acids (VFA). Organic matter degradability (OMD) and protozoa were also determined; in vitro gas production was also recorded and CH4 concentration was measured. Compared to the control, CH4 production was significantly lower for ethyl acetate extract (P<0.05), but higher for n-butanol and aqueous extracts. Production of ammonia- N was lower in all Eucalyptus extracts (P<0.05). Propionate production (P<0.05) increased for ethyl acetate and n-butanol extracts, whereas no effect was registered for VFA, for all Eucalyptus extracts. Ethyl acetate extract decreased in vitro OMD (P<0.05), whereas n-butanol and aqueous extracts were comparable to the control. Protozoa population decreased (P<0.05) for all extracts in comparison with the control. Eucalyptus ethyl acetate extract might be promising to be used as a potent anti-methanogenic additive. Moreover, the assessment of the right dosage seems to be important to decrease methane production, without reducing feed nutritional value.

Download Full-text

In Vitro and In Vivo Anti-Inflammatory Activity of the Rhizomes of Globba pendula Roxb

Natural Product Communications ◽

10.1177/1934578x211055907 ◽

2021 ◽

Vol 16 (10) ◽

pp. 1934578X2110559

Author(s):

Le Minh Ha ◽

Ngo Thi Phuong ◽

Nguyen Thi Thu Hien ◽

Pham Thi Tam ◽

Do Thi Thao ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Inhibitory Effect ◽

Potential Effect ◽

Inflammatory Activity ◽

No Production ◽

Anti Inflammatory ◽

Inflammatory Effect

In this study, we aimed at evaluating in vitro and in vivo anti-inflammatory activity of various extracts of the rhizomes of Globba pendula Roxb. Three extracts ( n-hexane, ethyl acetate, and water) were screened for their inhibitory effect on NO production by lipopolysaccharide-stimulated RAW 264.7 macrophages. The ethyl acetate extract of G. pendula rhizomes (EGP) showed a potential effect with an IC50 value of 32.45 µg/mL. For in vivo study, the ethyl acetate extract was further investigated for its anti-inflammatory effect using collagen antibody-induced arthritic mice (CAIA). The level of arthritis in experimental mice significantly reduced ( P < .05) after treatment with EGP at a dose of 500 mg/kg body weight (b.w.). This study also revealed that EGP is orally non-toxic. Ethyl p-methoxy cinamate was identified as the main constituent of EGP, which may result in its anti-inflammatory effect.

Download Full-text

Biomedical applications of fish gut associated Streptomyces maritimus IM20 isolated from Rastrelliger kanagurta (Indian mackerel)

Research Journal of Biotechnology ◽

10.25303/1610rjbt120130 ◽

2021 ◽

Vol 16 (10) ◽

pp. 120-130

Author(s):

Angamuthu Vignesh ◽

Venugopal Gopikrishnan ◽

Sivaraj Anbarasu ◽

Manikkam Radhakrishnan ◽

Joseph Jerrine

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Radical Scavenging ◽

Bioactive Metabolites ◽

Molecular Characteristics ◽

Inhibition Assay ◽

Indian Mackerel ◽

Fish Gut ◽

Rastrelliger Kanagurta

The biomedical potential of fish gut-associated actinobacteria isolated from the marine fish Rastrelliger kanagurta (Indian mackerel) was investigated. The actinobacterial strain IM20 was isolated from the fish gut by using Kusters agar medium prepared with 50% sea water. Based on their phenotypic and molecular characteristics, strain IM20 was identified as Streptomyces maritimus. The bioactive metabolites produced from the strain IM20 by agar surface fermentation and ethyl acetate extraction were tested for in vitro antimicrobial, antiquorum sensing , anti-biofilm, anti TB, anti-oxidant and anti-cancer activity. The MIC value of ethyl acetate extract (EAE) of IM20 was found to be 16 μg ml−1 against S. aureus and E. coli. In quorum sensing inhibition assay, the extract showed violacein inhibition upto 87% at 512 μg ml−1 concentration when tested by pigment inhibition assay using C. violaceum MTCC 2656. The results of in vitro assays revealed that the ethyl acetate extract of IM-20 (EAE-IM20) showed 75%, 83% and 72% inhibition against Mycobacterium tuberculosis H37Rv, M. tuberculosis (SHRE sensitive) and multi drug resistant (MDR) M tuberculosis respectively at 500 μg ml−1 concentration. In DPPH assay, 71% radical scavenging activity was exhibited by the EAE-IM20 at 250 μg ml−1. In MTT assay, EAEIM20 exhibited 74.29±2.01% inhibition on breast cancer cell line MCF7 (250 μg ml−1). This study broadly determines that the fish associated actinobacteria is a prolific place for diverse multifunctional bioactive compounds for the development of medically important unique drugs.

Download Full-text

In Vitro Screening for Cytotoxic, Anti-bacterial, Anti-HIV1-RT Activities and Chemical Constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii

The Natural Products Journal ◽

10.2174/2210315511666210119125611 ◽

2021 ◽

Vol 11 ◽

Author(s):

Charina Worarat ◽

Wilart Pompimon ◽

Phansuang Udomputtimekakul ◽

Sukee Sukdee ◽

Punchavee Sombutsiri ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Biological Activities ◽

Chemical Constituents ◽

Bacterial Activity ◽

Cytotoxic Activities ◽

Separation And Purification ◽

Crude Extracts ◽

Cytotoxicity Activities

Background: Although the chemical constituents and biological activities of a large number of plants in the Croton genus have been studied, there are still recently discovered plants to be investigated. Objective: 1. To investigate the anti-bacterial, anti-HIV1-RT, and cytotoxicity activities of crude extracts from these plants. 2. To investigate the chemical constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii. Method: The anti-bacterial, anti-HIV1-RT, and cytotoxicity of the three plants were evaluated by standard techniques. Extraction, separation, and purification of extracts from the three plants were undertaken. Results: The ethyl acetate extract of C. fluviatilis showed low anti-bacterial activity against E. aerogenes, E. coli 0157: H7, and P. mirabilis, together with the ethyl acetate extract of C. acutifolius displayed low anti-bacterial activity against E. aerogenes, while all the crude extracts of C. thorelii were inactive. The ethyl acetate extracts of C. thorelii, and C. fluviatilis showed strong inhibited HIV1-RT, whereas the ethyl acetate extract of C. acutifolius, and the hexane extract of C. fluviatilis displayed moderate inhibited HIV1-RT. Cytotoxic properties of three Croton plants were specific to KKU-M213, MDA-MB-231, A-549, and MMNK-1. Especially, the ethyl acetate extract of C. acutifolius exhibited strong cytotoxic activities against MDA-MB-231, A-549, and MMNK-1. Furthermore, the ethyl acetate extract of C. thorelii showed high cytotoxic activities against KKU-M213, and MDA-MB-231. Compounds 1, and 4 were found in C. fluviatilis. Compounds 2 and 4 were also found in C. acutifolius. Moreover, compound 3 was only found in C. thorelii. Conclusion: The present study revealed that the three Croton species are good sources of flavonoid compounds and further investigation of the chemical constituents from these plants may prove to be fruitful to discover more active compounds to be tested as potential medicines.

Download Full-text

Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

E3S Web of Conferences ◽

10.1051/e3sconf/202015101029 ◽

2020 ◽

Vol 151 ◽

pp. 01029

Author(s):

Nuzul Asmilia ◽

Mahdi Abrar ◽

Yudha Fahrimal ◽

Amalia Sutriana ◽

Yobeswi Husna

Keyword(s):

Ethyl Acetate ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Positive Control ◽

Hexane Extract ◽

Clear Zone ◽

Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

Download Full-text

EVALUATION OF THE WOUND HEALING POTENTIAL OF OINTMENT PREPARATION OF ETHYL-ACETATE EXTRACT OF MORINGA OLEIFERA (LAM) IN RATS

African Journal of Traditional Complementary and Alternative Medicines ◽

10.21010/ajtcamv15i3.8 ◽

2018 ◽

Vol 15 (3) ◽

pp. 64

Author(s):

Morenike Coker ◽

Grace Adejo ◽

Benjamin Emikpe ◽

Victor Oyebanji

Keyword(s):

Wound Healing ◽

Ethyl Acetate ◽

Moringa Oleifera ◽

Wound Closure ◽

Ethyl Acetate Extract ◽

Antibacterial Property ◽

Closure Rate ◽

Prolonged Contact ◽

Ointment Base

Background: This study examined the in vitro antibacterial property of extracts of Moringa oleifera and the effect of different concentrations of the ethyl-acetate extract on cutaneous wound using an ointment delivery base. The aim of the study was to screen the extracts with best antibacterial property and evaluate effectiveness of different concentrations of the best extract when delivery is modified to ensure prolonged contact and reduced frequency of administration using an ointment base delivery vehicle. Materials and Methods: Dried and pulverized leaves of Moringa oleifera were screened for secondary metabolites. Successive gradient extraction was carried out using n-hexane, ethyl acetate and methanol. Leaf extracts were screened against clinical wound isolates of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus mirabilis using agar cup diffusion technique. The ethyl acetate extract was investigated for its healing efficiency on excision wound model in rats. The study made use of wistar rats (150-180 kg), randomized into Gentamicin, M. oleifera ointment (5% and 3.25%), and ointment base treatment groups. Treatments were applied topically on days 0, 7 and 14. The percentage wound closure rate was measured and histopathology of the healed wounds carried out. Results: In vitro antimicrobial screening showed that ethyl-acetate extract was effective against the test isolates. Topical application of ointment with 3.25% of the plant extract resulted in faster wound closure rate, rapid epithelization, resolution of granulation tissue, and remodeling at histology. Conclusion: This may be due to less interference by components of M. oleifera which appear to retard wound healing at higher concentrations. Therefore, M. oleifera 3.25% ointment preparation is recommended topically for wound healing.

Download Full-text