Assessment of the Genotoxicity of Two Agricultural Residues After Processing by Diplopods Using the Allium cepa Assay

2013 ◽  
Vol 224 (4) ◽  
Author(s):  
Cintya A. Christofoletti ◽  
Janaína Pedro-Escher ◽  
Carmem S. Fontanetti
Keyword(s):  
Allium Cepa ◽  
Agricultural Residues ◽  
Allium Cepa Assay

Genotoxic effect of fruit extract of wild and cultivated cucurbits using Allium cepa assay

2021 ◽  
pp. 1-13
Author(s):  
Rupinderpal Kaur ◽  
Yumnam Devashree ◽  
Ravindra Kumar ◽  
Saurabh Gupta ◽  
Mohd. Saleem Wani ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Genotoxic Effect ◽  
Fruit Extract ◽  
Allium Cepa Assay

scholarly journals Genotoxicity of aqueous extracts of Tulbaghia violacea as determined through an Allium cepa assay

2018 ◽  
Vol 115 (1/2) ◽  
Author(s):  
Lerato N. Madike ◽  
Samkeliso Takaidza ◽  
Cornelius Ssemakalu ◽  
Michael Pillay
Keyword(s):  
Traditional Medicine ◽  
Allium Cepa ◽  
Fungal Infections ◽  
Positive Control ◽  
Negative Control ◽  
Genotoxic Effects ◽  
Water Extracts ◽  
Root Extracts ◽  
Allium Cepa Assay ◽  
Chromosome Bridges

Tulbaghia violacea (wild garlic) is commonly used in traditional medicine for the treatment of various ailments including fungal infections, gastrointestinal ailments, asthma, fever, colds and pulmonary tuberculosis. We assessed the potential genotoxic effects of water extracts from the leaves, stems and roots of T. violacea using the Allium cepa assay. Extracts at concentrations of 100, 250, 500 and 1000 μg/mL were tested on root meristems of A. cepa. Ethidium bromide was used as a positive control whereas distilled water acted as a negative control. The results reveal that as the concentrations of the water extracts of T. violacea increased, the mitotic indices decreased. Similarly, the percentage of chromosomal aberrations was dependent on the concentration as well as on which part of the plant was used. The six most common chromosome aberrations included laggard chromosomes, chromosome bridges, c-mitosis, sticky chromosomes, formation of binuclei and formation of trinuclei. The presence of micronucleated cells at interphase also increased as the concentration of the water extracts increased. The results confirm that water extracts of T. violacea exert significant genotoxic effects at higher concentrations, with the stem extracts being more toxic than the leaf and root extracts at similar concentrations. Significance: • Water extracts of T. violacea – a plant commonly used in traditional medicine – were found to have significant genotoxic effects at higher concentrations.

scholarly journals Allium cepa assay based comparative study of selected vegetables and the chromosomal aberrations due to heavy metal accumulation

2020 ◽  
Vol 27 (5) ◽  
pp. 1368-1374 ◽  
Author(s):  
Maria Sabeen ◽  
Qaisar Mahmood ◽  
Zulfiqar Ahmad Bhatti ◽  
Faridullah ◽  
Muhammad Irshad ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Comparative Study ◽  
Allium Cepa ◽  
Chromosomal Aberrations ◽  
Metal Accumulation ◽  
Heavy Metal Accumulation ◽  
Allium Cepa Assay

Genotoxicity assessment of raw and treated water samples using Allium cepa assay: evidence from Perak River, Malaysia

2017 ◽  
Vol 25 (6) ◽  
pp. 5421-5436 ◽  
Author(s):  
Amirhossein Malakahmad ◽  
Teh Sabariah Binti Abd Manan ◽  
Subarna Sivapalan ◽  
Taimur Khan
Keyword(s):  
Allium Cepa ◽  
Water Samples ◽  
Treated Water ◽  
Allium Cepa Assay

Study of Anti-oxidant, Anti-inflammatory, Genotoxicity, and Antimicrobial Activities and Analysis of Different Constituents found in Rhizome Essential Oil of Curcuma caesia Roxb., Collected from North East India

2020 ◽  
Vol 21 (5) ◽  
pp. 403-413 ◽  
Author(s):  
Manabi Paw ◽  
Roktim Gogoi ◽  
Neelav Sarma ◽  
Sudin K. Pandey ◽  
Angana Borah ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Allium Cepa ◽  
Antimicrobial Activities ◽  
Commercial Application ◽  
Diffusion Method ◽  
Ms Analysis ◽  
Allium Cepa Assay ◽  
Anti Inflammatory ◽  
Mic Value

Background: This investigation was designed to evaluate the chemical composition, antioxidant, anti-inflammatory, genotoxicity, and antimicrobial activities of Curcuma caesia Roxb rhizome essential oil. Methods: Gas Chromatography/Mass Spectroscopy (GC/MS) analysis was performed to determine the chemical composition, standard antioxidative test DPPH assay, reducing power assay, in vitro antiinflammatory activity (egg albumin denaturation, protease inhibitory assay) by using standard methods. Similarly, antimicrobial activity was tested using the disc diffusion method, minimum inhibitory concentration ability (MIC); while to test genotoxicity, Allium cepa assay was used. Results: GC/MS analysis revealed eucalyptol (28.55%), epicurzerenone (19.62%), and camphor (21.73%) as the major components of C. caesia rhizome essential oil. Potent antioxidant (IC50= 48.08±0.003 μg/mL), anti-inflammatory (IC50= 121.7±0.0013 μg/mL), and antimicrobial activities of the essential oil were recorded better than the standard drugs Fluconazole for fungus and Ciprofloxacin for bacteria. The essential oil also possessed a strong antibacterial effect against two tested bacterial strains B. subtilis and B. cereus with 7.5 μg/mL MIC value, while for fungal strains the essential oil was most effective against S. cereviaceae with an MIC value of 2.5 μg/mL. All the data were recorded in triplicates. Allium cepa assay revealed minor genotoxicity with mitotic index, MI= 27.70%; chromosome aberration, A= 1.1% of C. caesia rhizome essential oil. Conclusion: C. caesia rhizome essential oil possesses potent antioxidant, anti-inflammatory, and antimicrobial properties with negligible genotoxicity. Hence, the present study is highly significant for the utilization of rhizome of C. caesia, a high-value ethnopharmacological plant for advanced R & D and commercial application.

Cytotoxic and Genotoxic Effects of Cassava Effluents using the Allium cepa Assay

2011 ◽  
Vol 1 (1) ◽  
pp. 1-9 ◽  
Author(s):  
D.I. Olorunfemi ◽  
G.E. Okoloko ◽  
A.A. Bakare ◽  
A. Akinboro
Keyword(s):  
Allium Cepa ◽  
Genotoxic Effects ◽  
Allium Cepa Assay

scholarly journals Safety evaluation of green synthesized Cola nitida pod, seed and seed shell extract-mediated silver nanoparticles (AgNPs) using an Allium cepa assay

2017 ◽  
Vol 11 (6) ◽  
pp. 895-909 ◽  
Author(s):  
Taofeek A. Yekeen ◽  
Musibau A. Azeez ◽  
Akeem Akinboro ◽  
Agbaje Lateef ◽  
Tesleem B. Asafa ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Allium Cepa ◽  
Safety Evaluation ◽  
Allium Cepa Assay ◽  
Cola Nitida

scholarly journals Genotoxicity of Euphorbia hirta: An Allium cepa Assay

Molecules ◽  
2012 ◽  
Vol 17 (7) ◽  
pp. 7782-7791 ◽  
Author(s):  
Kwan Yuet Ping ◽  
Ibrahim Darah ◽  
Umi Kalsom Yusuf ◽  
Chen Yeng ◽  
Sreenivasan Sasidharan
Keyword(s):  
Allium Cepa ◽  
Euphorbia Hirta ◽  
Allium Cepa Assay
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