Correlation between the positive rate of SAA in children with respiratory tract infection and ambient temperature

Abstract Aim: The hospitalized children with Mycoplasma pneumonia (M. pneumonia) infection caused by respiratory tract infection in Chengdu were studied and analysis of the epidemiological characteristics was carried out to provide a theoretical basis for clinical diagnosis and treatment.Method: 22882 hospitalized children with respiratory tract infections between January 2014 and December 2020 were collected M. pneumonia IgM antibody was detected by indirect immunofluorescence method and passive agglutination method. Demographic characteristics, clinical diagnose and laboratory data of these children were analyzed.retrospectively.. Result : The 4213 specimens with M. pneumonia were tested positive, the total positive rate was18.41%(18.30% in male and 22.72% in female). Higher positive rates were found in female children,Look from the statistical analysis results, the consistency between the two sets of data is low（ｘ2=198.078、P＜0.01）. The results of different age patients with contrast different M. pneumonia infection degree were statistically significant（F=162.7532、P＜0.01）,there was higher M. pneumonia positive rate in Preschoolers and school-age children ,33.98% and 32.98%, respectively.The incidence rate of M. pneumonia in 2017 and 2019 was significantly higher than average （F=538.95, P＜0.01)The difference of incidence rate of M. pneumonia was not significant in different months in 2014, 2015 and 2020 (P>0.05). But the probability of M .pneumonia infection patients was much higher from April to May and September to October in2016,2017,2018 and 2019（P＜0.05）. There was no correlation about M. pneumonia infection with temperature and humidity( P＞0.05)，there was negative correlation with PM2.5（R=0.09362, P<0.01）and PM10.（R=0.1185, P<0.01）.There was no difference about constituent ratio of case of M. pneumonia infection between 2014 and 2019 (F=32.34,P>0.05).The Common respiratory diseases of M. pneumonia infection, bronchopneumonia accounts for the highest proportion,followed the exacerbation of asthma and severe pneumonia.There was significantly difference about constituent ratio of case of M. pneumonia infection between in 2020 and in other years (F=159.35,P<0.01) .The Common respiratory diseases of M. pneumonia infection, bronchopneumonia accounts for the highest proportion,followed the acute bronchitis and exacerbation of asthma.Conclusion:The distribution and epidemiological trend of M. pneumonia in patients with respiratory tract infection showed the risk of inflammation was connected with the gender, age, year and month, no relationship with temperature and humidity in Chengdu,.Higher M. pneumonia positive rate was shown in the children with bronchial pneumonia and exacerbation of asthma.The prevention measures which controlled the COVID-19 disease had effectively controlled the infection rate of M. pneumonia.

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Clinical Value of Two Detection Methods for Mycoplasma Pneumoniae Antibody in the Diagnosis of Mycoplasma Pneumoniae Infection in North China

10.21203/rs.3.rs-934275/v1 ◽

2021 ◽

Author(s):

Jupeng Wang ◽

Lina Zhu ◽

Mingkun Ma ◽

Hui Chen ◽

Suxiang Guo ◽

...

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Mycoplasma Pneumoniae ◽

North China ◽

Detection Methods ◽

Antibody Detection ◽

Positive Rate ◽

Adults And Children ◽

Males And Females

Abstract Background: To analyze the detection of Mycoplasma pneumoniae in adults and children in North China using two antibody detection methods, and to guide prevention and treatment.Methods: A retrospective study was conducted from September 2017 to May 2021. Mycoplasma pneumoniae total antibody was detected using particle agglutination (PA). Anti-Mycoplasma pneumoniae IgM in patients with respiratory tract infection was detected by indirect immunofluorescence. All patients were divided into 9 groups according to age: ≤1, 2-3, 4-6, 7-14, 15-18, 19-39, 40-59, 60-79, ≥80,Results: The positive rate of Mycoplasma pneumoniae total antibody in 5,666 patients with community-acquired pneumonia was 40.13%. In adults and children, the positive rates were 19.92% and 77.3% (p<0.05), respectively. The positive rates in males and females was 37.89% and 42.40% (p<0.05), respectively. The positive rate for anti-Mycoplasma pneumoniae IgM in 5,746 patients with respiratory tract infection was 28.56%, and 10.37% and 36.82% in adults and children (p<0.05), respectively. In males and females, the positive rate for anti-Mycoplasma pneumoniae IgM was 24.56% and 33.38% (p<0.05). The highest positive rates for total antibody and anti-Mycoplasma pneumoniae IgM were recorded in autumn. Of the 1,975 patients tested for Mycoplasma pneumoniae antibody using both methods simultaneously, 26.71% were negative for total antibody and 8.63% had titers ranging between 1:40 and 1:80 when positive for IgM antibody. When negative for anti-Mycoplasma pneumoniae IgM, total antibody titer was ≥1:160 in 34.94% of the patients.Conclusion: Mycoplasma pneumoniae is the main cause of respiratory tract infection and its incidence is highest in autumn. Because Mycoplasma pneumoniae was more commonly detected in women and children, screening should be strengthened in these groups.

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Epidemiology and Molecular Identification of Mycoplasma pneumoniae associated respiratory tract, Zhejiang province, China, 2008-2017

10.21203/rs.2.12040/v1 ◽

2019 ◽

Author(s):

Qian Jiang ◽

Zhao Zhao ◽

Fangfang Yang ◽

Yumei Ge

Keyword(s):

Tract Infection ◽

Early Diagnosis ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Mycoplasma Pneumoniae ◽

Acute Respiratory Tract Infection ◽

Zhejiang Province ◽

Taqman Probe ◽

Positive Rate ◽

Tract Infections

Abstract Background Mycoplasma pneumoniae is a common cause of respiratory infections in humans. The aim of this study was to investigate the infection of Mycoplasma pneumoniae (MP) in patients with acute respiratory tract infections in Zhejiang Province from 2008 to 2017, and to provide evidence for the early diagnosis and prevention of MP pneumonia. Methods MP-DNA was detected in nasopharyngeal swabs of patients with acute respiratory tract infection by real-time fluorescent PCR (Taqman probe). Statistical analysis and epidemiological investigation were carried out on the test results. Results There were 10296 patients with acute respiratory tract infection in Zhejiang People's Hospital from 2008 to 2017, including 4387 females and 5909 males. A total of 1251 MP-DNA positive patients were detected, with a total positive rate of 12.2% (1251/10296). Among 1251 patients with M. pneumoniae infection, 571 were female positive, with an average positive rate of 13.0% (571/4387), 680 were male positive, with a positive rate of 11.5% (680/5909). From 2008 to 2017, the positive rates were 22.8% (33 cases), 20.9% (211 cases), 20.9% (350 cases), 5.5% (70 cases), 11.7% (136 cases), 15.2% (190 cases), 7.8% (94 cases), 5.9% (62 cases), 7.8% (56 cases) and 6.0% (49 cases), respectively. Of 1251 MP-DNA positive patients, 1243 (99.4%) were younger than 18 years old. Conclusions MP infection mainly occurs in the age below 18 years old, suggesting that early diagnosis and prevention of MP infection in adolescents should be emphasized.

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Characteristics and Antibiotic Resistance of Haemophilus influenzae in Children with Lower Respiratory Tract Infection in Chengdu, China

Jundishapur Journal of Microbiology ◽

10.5812/jjm.114210 ◽

2021 ◽

Vol 14 (2) ◽

Author(s):

Qin Wang ◽

Cheng-Gui Liu ◽

Jian Xu ◽

Qin Zhang ◽

Chong-Hui Zhao ◽

...

Keyword(s):

Antibiotic Resistance ◽

Tract Infection ◽

Respiratory Tract ◽

Haemophilus Influenzae ◽

Respiratory Tract Infection ◽

Lower Respiratory Tract Infection ◽

Lower Respiratory Tract ◽

Detection Rate ◽

Human Respiratory Tract ◽

Positive Rate

Background: Haemophilus influenzae is an opportunistic pathogen of the human respiratory tract. Haemophilus influenzae can cause not only respiratory tract infection in children but also otitis media, epiglottitis and sinusitis. With the widespread use of antibiotics, the positive rate of β-lactamase in H. influenzae is increasing, and the rate of antimicrobial resistance is also increasing, which increases the difficulty of clinical treatment. Objectives: To study the infection characteristics of patients and the antibiotic resistance of H. influenzae in lower respiratory tract samples of children in Chengdu, so as to provide a reference for its clinical diagnosis and the rational use of antibiotics. Methods: Sputum samples of 15891 children aged 0-14 years with lower respiratory tract infection were collected. Haemophilus influenzae was cultured and identified, its drug susceptibility tested, and the results determined according to the guidelines of CLSI 2020. Results: A total of 15891 clinical isolate strains in sputum were detected for drug sensitivity from December 2018 to January 2020, of which 5488 were H. influenzae, accounting for 34.54% (5488/15891). The sex of children infected with H. influenzae was not skewed (P > 0.05). The detection rate of H. influenzae was the highest in children aged 7 - 11 months, and the lowest was in the age group ≤ 28 d. The detection rate was the highest in spring and the lowest in autumn. The positive rate of β-lactamase was 92.0%, the resistance rate to ampicillin was 92.0%, the sensitivity to amoxicillin/clavulanate was 70.2%, and the sensitivity to cefotaxime, ofloxacin, tetracycline, chloramphenicol, and rifampicin was more than 90.0%. Conclusions: Children aged 7 months to 14 years were generally susceptible to H. influenzae in spring, and the positive rate of β-lactamase was high. Doctors should refer to the infection characteristics and drug resistance of H. influenzae and choose antibiotics correctly to better control the infection.

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