Making Good Use of Food Wastes: Green Synthesis of Highly Stabilized Silver Nanoparticles from Grape Seed Extract and Their Antimicrobial Activity

2014 ◽  
Vol 10 (1) ◽  
pp. 12-18 ◽  
Author(s):  
Hui Xu ◽  
Lei Wang ◽  
Hongyan Su ◽  
Liang Gu ◽  
Tingting Han ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Food Wastes

scholarly journals Effectiveness of grape seed extract-based intracanal dressings against Enterococcus Faecalis and its influence on dentin microhardness and bond strength of filling material

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Matheus Albino Souza ◽  
Daniel De Lima Dalla Lana ◽  
Amália Pletsch ◽  
Huriel Scartazzini Palhano ◽  
Juliane Bervian ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bond Strength ◽  
Enterococcus Faecalis ◽  
Storage Period ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Filling Material ◽  
Bacterial Reduction ◽  
Significant Difference

The aim of this study was to evaluate the effectiveness of grape seed extract (GSE)-based intracanal dressings against Enterococcus faecalis (E. faecalis) and its influence on dentin microhardness and bond strength of the filling material. The root canals of 126 human teeth were distributed into three test groups: antimicrobial activity (60 teeth), dentin microhardness (30 teeth) and bond strength (36 teeth). In all three groups, specimens were subdivided into six groups, according to intracanal dressing protocols: G1 – distilled water (DW); G2 – 2% chlorhexidine gel (CHX); G3 – calcium hydroxide (Ca[OH]2)+DW; G4 – GSE+DW; G5 – Ca(OH)2+CHX; G6 – GSE+CHX. The counting of colony-forming units (CFUs), the Vickers microhardness tester and the push-out test were performed to evaluate the antimicrobial activity, dentin microhardness and bond strength, respectively. Specific statistical analysis was performed for each evaluation (α=5%). The greatest bacterial reduction was observed in G5 (Ca[OH]2+CHX) and G6 (GSE+CHX) (p<0.05). There was no statistically significant difference among groups in the dentin microhardness evaluation (p<0.05). The highest bond strength in the immediate evaluation was observed in G4 (GSE+DW) and G6 (GSE+CHX), whereas the highest bond strength after 12 months of storage was observed in G2 (CHX), G3 (Ca[OH]2+DW), G4 (GSE+DW), and G6 (GSE+CHX) (p<0.05). After the storage period, bond strength was increased in G2 (CHX) and G3 (Ca[OH]2+DW), and remained unchanged in G4 (GSE+DW) and G6 (GSE+CHX) (p<0.05). GSE-based intracanal dressings have antimicrobial potential against E. faecalis, have no influence in dentin microhardness and preserve the high bond strength of filling materials for root dentin over time.

scholarly journals Growth Media Affect Assessment of Antimicrobial Activity of Plant-Derived Polyphenols

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Xin Xu ◽  
Zhen M. Ou ◽  
Christine D. Wu
Keyword(s):  
Antimicrobial Activity ◽  
Methyl Salicylate ◽  
Antimicrobial Agents ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Polyphenolic Compounds ◽  
Growth Media ◽  
Complex Media ◽  
Oral Microorganisms

This study aimed to investigate the effects of different microbial growth media on the laboratory assessment of antimicrobial activity of natural polyphenolic compounds. The inhibition of the tea polyphenol EGCG on growth of selected oral microorganisms was evaluated in complex media and a protein-free chemically defined medium (CDM). Other antimicrobial agents (polyphenolic grape seed extract, plant alkaloid berberine, methyl salicylate, and chlorhexidine gluconate) were also tested in the study. The presence of proteins and their effects on the antimicrobial activity of EGCG were investigated by the addition of BSA to the CDM. The MICs of EGCG against test oral microorganisms were 4 to 64 times higher in complex media than in CDM. The polyphenolic grape seed extract exhibited similar discrepancies. However, the MICs of the nonpolyphenolic compounds (berberine, methyl salicylate, and chlorhexidine) were not significantly different between the two growth media. The MIC of EGCG againstS. mutansUA159 in CDM with added BSA was 16 times higher than that in CDM alone. Therefore, nonproteinaceous CDM should be used to avoid interference of proteins with the active ingredients when testing the antimicrobial activity of plant-derived polyphenolic compounds against microorganisms. This will also minimize the discrepancies noted in results obtained by different investigators.

scholarly journals Assessment of antimicrobial activity of sodium hypochlorite, calcium hypochlorite and grape seed extract against Enterococcus faecalis

2018 ◽  
Vol 32 (3) ◽  
pp. 136
Author(s):  
Larissa Taís Soligo ◽  
Doglas Cecchin ◽  
Matheus Albino Souza ◽  
Ezequiel Santin Gabrielli ◽  
Huriel Scartazzini Palhano ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Saline Solution ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
The Other ◽  
Diffusion Method ◽  
Calcium Hypochlorite

OBJECTIVE: This study aimed to assess the antimicrobial action of calcium hypochlorite [Ca(OCl)2] at concentrations of 2.5% and 6%, and of grape seed extract (GSE) at concentrations of 10%, 30%, and 50%, against Enterococcus faecalis, comparing it to the action of 6% sodium hypochlorite (NaOCl).METHODS: Saline solution was used as negative control. The inhibition halos of microbial growth were verified by the agar disk diffusion method. Twelve Petri plates were used for seeding with culture medium of approximately 5 mm in thickness. In each plate, 5 disks of pure and sterile antibiogram, soaked in the substances to be tested, were used and taken to the plate containing the seededbacterial strain. The plates remained in aerobic bacteriological incubator for 24 h at 37°C temperature. After 24 h, the inhibition halos were measured with a digital caliper. Analysis of Variance (ANOVA) was used for statistical analysis followed by Tukey's complementary test, at 5% significance.RESULTS: The 6% Ca(ClO)2 presented inhibition halo statistically higher than the other solutions (p<0.05), followed by 2.5% Ca(ClO)2, which was statistically similar to 6% NaOCl (p>0.05). The GSE concentrations resulted in lower inhibition halos of active substances and the different concentrations were similar to each other. Lastly, saline solution presented the same inhibition halos in all groups (p<0.05).CONCLUSION: It may be concluded that 6% Ca(OCl)2 presented higher antimicrobial activity than 6% NaOCl. On the other hand, all GSE concentrations were lower than NaOCl and Ca(OCl)2.

scholarly journals Antimicrobial activity of intracanal dressings composed by natural products associated to chlorhexidine and its influence on dentinal colour change

2020 ◽  
Vol 25 (1) ◽  
pp. 42-49
Author(s):  
Matheus Albino Souza ◽  
Huriel Scartazzini Palhano Palhano ◽  
Bárbara Facco Rauber Facco Rauber ◽  
Lourenço Luís Albarello ◽  
Guilherme Lima ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Colour Change ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Bacterial Reduction ◽  
Ginger Extract ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Colour Changes

Purpose: The aim of study was to evaluate the antimicrobial activity of intracanal dressings and their influence on dentinal colour changes. Material and methods: Eighty samples were divided into eight groups (n=10) according to intracanal dressing protocols: G1–distilled water (DW); G2–2% chlorhexidine gel (CHX); G3–calcium hydroxide (Ca[OH]2)+DW; G4–grape seed extract (GSE)+DW; G5–ginger extract (GE)+DW; G6–Ca(OH)2+CHX; G7–GSE+CHX; and G8–GE+CHX. The antimicrobial activity was evaluated by colony-forming units (CFUs) counting and dentinal colour changes was evaluated by digital spectrophotometry. Data were statistically analysed by specific tests (α=0.05). Results: The highest bacterial reduction was observed in groups 4, 6, 7 and 8, with no significant difference between them (p<0.05). Groups 4 and 7 showed the highest medians of dentinal colour change (p<0.05). Conclusion: The addition of CHX improved the antimicrobial activity of GE-based intracanal dressing, with no effect in GSE-based intracanal dressing; moreover, these protocols induced significant dentinal colour changes.

Grape Seeds Extract as Brain Food: A Review

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Souad El Gengaihi ◽  
Doha H. Abou Baker
Keyword(s):  
Antioxidant Activities ◽  
Complex Mixture ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Amyloid Peptide ◽  
Grape Seeds ◽  
Potential Benefits ◽  
Reduced Risk

Interest in the biological role of bioactive compounds present in medicinal herbs has increased over the last years. Of particular interest are plants that have an anti-Alzheimer activities. Several plants can be useful for Alzheimer (AD) management. Such as these which have anti-inflammatory activity, acetylcholinesterase (AChE) inhibitory action, antiapoptotic, slow the aggregation of amyloid peptide and antioxidant activities. Grape seed extract (GSE) is a complex mixture of several compounds, mostly represented by polyphenols and flavonoids. Their consumption is safe and is recognized to exert several health benefits. GS flavonoids have been associated with the reduced risk of chronic diseases, we present some findings on the potential benefits of GSE for the treatment of AD.

Grape Seed Extract Complex (FFM-GS) Inhibits Postmenopausal Symptoms: In Vitro and In Vivo S

2019 ◽  
Vol 48 (3) ◽  
pp. 297-305
Author(s):  
Minhee Lee ◽  
Da-Eun Nam ◽  
Soo-Jeung Park ◽  
Dakyung Kim ◽  
Jeong-Moon Yun ◽  
...  
Keyword(s):  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Postmenopausal Symptoms

scholarly journals Comparative Evaluation of Immediate Bond Strength to Bleached Enamel Following Application of Various Antioxidant Solutions

2017 ◽  
Vol 05 (02) ◽  
pp. 084-089
Author(s):  
Alisha Dhingra ◽  
Ashu Gupta ◽  
Anshu Minocha ◽  
Nayantara Sen
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
The Other ◽  
Sodium Ascorbate ◽  
Group V ◽  
Group I ◽  
Significant Difference

Abstract Aim: The aim of this study was to compare the reversal of shear bond strength of composite to bleached enamel immediately after bleaching followed by application of various antioxidant solutions. Material and Methods: Seventy central incisors were divided into seven groups. Groups I and II served as unbleached and bleached controls respectively. Groups III, IV, V, VI and VII served as the experimental groups and were subjected to 37.5% hydrogen peroxide bleaching followed by 10 min application of 10% sodium ascorbate, 25% alpha-tocopherol, 6.5% grape seed extract, 5% lycopene and 5% green tea extract respectively. Following composite bonding, shear bond strength was determined and the results were analyzed using ANOVA followed by Post Hoc Multiple Comparisons test. Results: The bond strength values for Group I (positive control) were maximum and significantly different than all the other groups except Grape seed extract group (Group V). When compared to Group II (bleached control), all the groups showed significantly higher bond strength. Significant difference in the bond strength values were seen between Group III (10% sodium ascorbate) and Group V. Also values for Group V were significantly different from Group VI (5% lycopene). All the other values showed insignificantly different results. Conclusion: All the antioxidant solutions improved the shear bond strength values after bleaching but only Grape seed extract application reversed the values to the non bleached levels. Lycopene was least effective. Other antioxidants showed comparable results.

Sign in / Sign up

Export Citation Format

Share Document