scholarly journals Growth Media Affect Assessment of Antimicrobial Activity of Plant-Derived Polyphenols

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Xin Xu ◽  
Zhen M. Ou ◽  
Christine D. Wu
Keyword(s):  
Antimicrobial Activity ◽  
Methyl Salicylate ◽  
Antimicrobial Agents ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Polyphenolic Compounds ◽  
Growth Media ◽  
Complex Media ◽  
Oral Microorganisms

This study aimed to investigate the effects of different microbial growth media on the laboratory assessment of antimicrobial activity of natural polyphenolic compounds. The inhibition of the tea polyphenol EGCG on growth of selected oral microorganisms was evaluated in complex media and a protein-free chemically defined medium (CDM). Other antimicrobial agents (polyphenolic grape seed extract, plant alkaloid berberine, methyl salicylate, and chlorhexidine gluconate) were also tested in the study. The presence of proteins and their effects on the antimicrobial activity of EGCG were investigated by the addition of BSA to the CDM. The MICs of EGCG against test oral microorganisms were 4 to 64 times higher in complex media than in CDM. The polyphenolic grape seed extract exhibited similar discrepancies. However, the MICs of the nonpolyphenolic compounds (berberine, methyl salicylate, and chlorhexidine) were not significantly different between the two growth media. The MIC of EGCG againstS. mutansUA159 in CDM with added BSA was 16 times higher than that in CDM alone. Therefore, nonproteinaceous CDM should be used to avoid interference of proteins with the active ingredients when testing the antimicrobial activity of plant-derived polyphenolic compounds against microorganisms. This will also minimize the discrepancies noted in results obtained by different investigators.

scholarly journals Effectiveness of grape seed extract-based intracanal dressings against Enterococcus Faecalis and its influence on dentin microhardness and bond strength of filling material

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Matheus Albino Souza ◽  
Daniel De Lima Dalla Lana ◽  
Amália Pletsch ◽  
Huriel Scartazzini Palhano ◽  
Juliane Bervian ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bond Strength ◽  
Enterococcus Faecalis ◽  
Storage Period ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Filling Material ◽  
Bacterial Reduction ◽  
Significant Difference

The aim of this study was to evaluate the effectiveness of grape seed extract (GSE)-based intracanal dressings against Enterococcus faecalis (E. faecalis) and its influence on dentin microhardness and bond strength of the filling material. The root canals of 126 human teeth were distributed into three test groups: antimicrobial activity (60 teeth), dentin microhardness (30 teeth) and bond strength (36 teeth). In all three groups, specimens were subdivided into six groups, according to intracanal dressing protocols: G1 – distilled water (DW); G2 – 2% chlorhexidine gel (CHX); G3 – calcium hydroxide (Ca[OH]2)+DW; G4 – GSE+DW; G5 – Ca(OH)2+CHX; G6 – GSE+CHX. The counting of colony-forming units (CFUs), the Vickers microhardness tester and the push-out test were performed to evaluate the antimicrobial activity, dentin microhardness and bond strength, respectively. Specific statistical analysis was performed for each evaluation (α=5%). The greatest bacterial reduction was observed in G5 (Ca[OH]2+CHX) and G6 (GSE+CHX) (p<0.05). There was no statistically significant difference among groups in the dentin microhardness evaluation (p<0.05). The highest bond strength in the immediate evaluation was observed in G4 (GSE+DW) and G6 (GSE+CHX), whereas the highest bond strength after 12 months of storage was observed in G2 (CHX), G3 (Ca[OH]2+DW), G4 (GSE+DW), and G6 (GSE+CHX) (p<0.05). After the storage period, bond strength was increased in G2 (CHX) and G3 (Ca[OH]2+DW), and remained unchanged in G4 (GSE+DW) and G6 (GSE+CHX) (p<0.05). GSE-based intracanal dressings have antimicrobial potential against E. faecalis, have no influence in dentin microhardness and preserve the high bond strength of filling materials for root dentin over time.

scholarly journals Antilisterial Effects of Gravinol-S Grape Seed Extract at Low Levels in Aqueous Media and Its Potential Application as a Produce Wash

2010 ◽  
Vol 73 (2) ◽  
pp. 266-273 ◽  
Author(s):  
BLEDAR BISHA ◽  
NATALIA WEINSETEL ◽  
BYRON F. BREHM-STECHER ◽  
AUBREY MENDONCA
Keyword(s):  
Antimicrobial Agents ◽  
Aqueous Media ◽  
Membrane Integrity ◽  
Viable Cell ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Live Cells ◽  
Cell Counts

Grape seed extract (GSE) is a rich source of proanthocyanidins, a class of natural antioxidants reported to have wide-ranging bioactivity as anti-inflammatory, anticarcinogenic, and antimicrobial agents. The ability of GSE to rapidly inactivate Listeria monocytogenes in vitro and the generally recognized as safe status of GSE make this extract an attractive candidate for control of Listeria in or on foods. Previously, GSE has been used at relatively high concentrations (1%) in complex food matrices and in combination with other antimicrobials. We sought to characterize the antilisterial effects of a commercial GSE preparation (Gravinol-S) alone at much lower concentrations (0.00015 to 0.125%) in aqueous solution and to test its possible use as an antimicrobial wash for fresh produce surfaces. Based on broth microdilution tests, the MICs of GSE against L. monocytogenes Scott A and Listeria innocua ATCC 33090 were as low as 50 and 78 μg ml−1, respectively. GSE was evaluated in 0.85% saline against live cells of L. innocua via flow cytometry, using propidium iodide as a probe for membrane integrity. At sub-MICs and after only 2 min of exposure, treatment with GSE caused rapid permeabilization and clumping of L. innocua, results that we confirmed for L. monocytogenes using fluorescence microscopy and Live/Dead staining. At higher concentrations (0.125%), GSE reduced viable cell counts for L. monocytogenes by approximately 2 log units within 2 min on tomato surfaces. These results suggest the potential for GSE as a natural control of Listeria spp. on low-complexity foods such as tomatoes.

scholarly journals Assessment of antimicrobial activity of sodium hypochlorite, calcium hypochlorite and grape seed extract against Enterococcus faecalis

2018 ◽  
Vol 32 (3) ◽  
pp. 136
Author(s):  
Larissa Taís Soligo ◽  
Doglas Cecchin ◽  
Matheus Albino Souza ◽  
Ezequiel Santin Gabrielli ◽  
Huriel Scartazzini Palhano ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Saline Solution ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
The Other ◽  
Diffusion Method ◽  
Calcium Hypochlorite

OBJECTIVE: This study aimed to assess the antimicrobial action of calcium hypochlorite [Ca(OCl)2] at concentrations of 2.5% and 6%, and of grape seed extract (GSE) at concentrations of 10%, 30%, and 50%, against Enterococcus faecalis, comparing it to the action of 6% sodium hypochlorite (NaOCl).METHODS: Saline solution was used as negative control. The inhibition halos of microbial growth were verified by the agar disk diffusion method. Twelve Petri plates were used for seeding with culture medium of approximately 5 mm in thickness. In each plate, 5 disks of pure and sterile antibiogram, soaked in the substances to be tested, were used and taken to the plate containing the seededbacterial strain. The plates remained in aerobic bacteriological incubator for 24 h at 37°C temperature. After 24 h, the inhibition halos were measured with a digital caliper. Analysis of Variance (ANOVA) was used for statistical analysis followed by Tukey's complementary test, at 5% significance.RESULTS: The 6% Ca(ClO)2 presented inhibition halo statistically higher than the other solutions (p<0.05), followed by 2.5% Ca(ClO)2, which was statistically similar to 6% NaOCl (p>0.05). The GSE concentrations resulted in lower inhibition halos of active substances and the different concentrations were similar to each other. Lastly, saline solution presented the same inhibition halos in all groups (p<0.05).CONCLUSION: It may be concluded that 6% Ca(OCl)2 presented higher antimicrobial activity than 6% NaOCl. On the other hand, all GSE concentrations were lower than NaOCl and Ca(OCl)2.

scholarly journals Adhesive systems modified with antimicrobial agents: a literature review

2020 ◽  
Author(s):  
Juliana Dias Aguiar ◽  
Michel Nicolau Youssef ◽  
Igor Studart Medeiros
Keyword(s):  
Antimicrobial Agents ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Additional Benefit ◽  
Antimicrobial Action ◽  
Copper Iodide ◽  
Adhesive Systems ◽  
Secondary Caries ◽  
Restoration Failure

Secondary caries is the primary cause of restoration failure. Thus, the development of adhesives with antimicrobial action is an advantageous option for their inhibition. However, this effect must be proven, as well as that the additional benefit does not interfere with material mechanical properties or biocompatibility. We analyzed adhesives with antimicrobial action by microbiological tests, bond strength, degree of conversion, and cytotoxicity. We analyzed 32 studies with commercially available antimicrobial adhesives (Clearfil™ SE Protect Bond/ MDPB, Gluma 2Bond/ glutaraldehyde, Peak Universal Bond/chlorhexidine), and experimental materials or commercial adhesives modified with antimicrobial agents, including materials with quaternary ammonium methacrylate (QAM) [dimethylaminododecyl methacrylate (DMADDM) and dimethylaminohexadecyl methacrylate (DMAHDM)], nanoparticles [silver (NAg), titanium dioxide (TiO2), zinc oxide (ZnO)], silver or zinc doped bioactive active glass (BAG), titanium, copper iodide, and compounds such as triclosan, quercetin, grape seed extract, among others. The use of antimicrobial agents is a favorable perspective for the functionalization of adhesive systems to inhibit secondary caries. However, more clinical studies need to prove the efficacy of these materials.

Health Perspectives of Grape Seed Extract (GSE)

2020 ◽  
pp. 88-98
Author(s):  
OJS Admin
Keyword(s):  
Fungal Infections ◽  
Wide Spectrum ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Food Supplement ◽  
Seed Extract ◽  
Polyphenolic Compounds ◽  
Vaginal Candidiasis ◽  
Grape Seeds ◽  
Positive Effects

From the last decade flavonoids have become very prominent in the studies related to grapes. It has also been outlined by the recent researches that the positive effects of the constituents derived from theresidues of grapes are due to the polyphenolic compounds. Grape Seed Extract (GSE) possess a wide spectrum of medicinal features to inhibit reaction of free radicals, prevent clumping of platelets, prevent inflammation etc. Apart from that GSE have gastro-protective, hepato-protective, and cardio-protective roles. It has been reveal that the residues derived from the seeds of the grapes with enhanced flavonol constituents are important to be utilized during the conditions of fungal infections as vaginal candidiasis due to candida preventing c h a r a c t e risti c s. Du e t o t h e p r e s e n c e o f proanthocyanidins grape seeds are used as a food supplement also.

Supercritical Fluid Chromatography of polyphenolic compounds in grape seed extract

2002 ◽  
Vol 55 (7-8) ◽  
pp. 417-421 ◽  
Author(s):  
A. Kamangerpour ◽  
M. Ashraf-Khorassani ◽  
L. T. Taylor ◽  
H. M. McNair ◽  
L. Chorida
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Polyphenolic Compounds

scholarly journals Antimicrobial activity of intracanal dressings composed by natural products associated to chlorhexidine and its influence on dentinal colour change

2020 ◽  
Vol 25 (1) ◽  
pp. 42-49
Author(s):  
Matheus Albino Souza ◽  
Huriel Scartazzini Palhano Palhano ◽  
Bárbara Facco Rauber Facco Rauber ◽  
Lourenço Luís Albarello ◽  
Guilherme Lima ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Colour Change ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Bacterial Reduction ◽  
Ginger Extract ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Colour Changes

Purpose: The aim of study was to evaluate the antimicrobial activity of intracanal dressings and their influence on dentinal colour changes. Material and methods: Eighty samples were divided into eight groups (n=10) according to intracanal dressing protocols: G1–distilled water (DW); G2–2% chlorhexidine gel (CHX); G3–calcium hydroxide (Ca[OH]2)+DW; G4–grape seed extract (GSE)+DW; G5–ginger extract (GE)+DW; G6–Ca(OH)2+CHX; G7–GSE+CHX; and G8–GE+CHX. The antimicrobial activity was evaluated by colony-forming units (CFUs) counting and dentinal colour changes was evaluated by digital spectrophotometry. Data were statistically analysed by specific tests (α=0.05). Results: The highest bacterial reduction was observed in groups 4, 6, 7 and 8, with no significant difference between them (p<0.05). Groups 4 and 7 showed the highest medians of dentinal colour change (p<0.05). Conclusion: The addition of CHX improved the antimicrobial activity of GE-based intracanal dressing, with no effect in GSE-based intracanal dressing; moreover, these protocols induced significant dentinal colour changes.

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