Radioimmunoscintigraphy of pancreatic cancer in tumor-bearing athymic nude mice using 99mtechnetium-labeled anti-KL-6/MUC1 antibody

2008 ◽  
Vol 26 (3) ◽  
pp. 133-139 ◽  
Author(s):  
Kenji Matsumura ◽  
Ichiro Niki ◽  
Hui Tian ◽  
Masahisa Takuma ◽  
Norio Hongo ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Nude Mice ◽  
Athymic Nude Mice ◽  
Tumor Bearing

scholarly journals TRPM2 promotes pancreatic cancer by PKC/MAPK pathway

2020 ◽  
Author(s):  
Rui Lin ◽  
Xunxia Bao ◽  
Hui Wang ◽  
Sibo Zhu ◽  
Zhongyan Liu ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Nude Mice ◽  
Transcriptome Analysis ◽  
Cell Model ◽  
Mapk Pathway ◽  
Tissue Sample ◽  
Pancreatic Cancer Cell Line ◽  
Cancer Cell Line ◽  
Migration And Invasion ◽  
Tumor Bearing

AbstractBackgroundThe mechanism of pancreatic cancer(PA) is not fully understanded. In our last report, TRPM2 plays a promising role in pancreatic cancer. However, the mechanism of TRPM2 is still unknown in this dismal disease. This study was designed to investigate the role and mechanism of TRPM2 in pancreatic cancer.MethodsTRPM2 overexpressed and siRNA plasmid were created and transfected with pancreatic cancer cell line(BxPC-3) to construct the cell model. We employed CCK-8, Transwell, scratch wound, and nude mice tumor bearing model to investigate the role of TRPM2 in pancreatic cancer. Besides, we collected the clinical data, tumor tissue sample(TT) and para-tumor sample(TP) from the pancreatic cancer patients treated in our hospital. We analyzed the mechanism of TRPM2 in pancreatic cancer by transcriptome analysis, Westernblot, and PCR.ResultsOverexpressed TRPM2 could promote pancreatic cancer in proliferation, migration, and invasion ability in no matter the cell model or nude mice tumor bearing model. TRPM2 level is highly negative correlated to the overall survival and progression-free survival time in PA patients, however, it is significantly increased in PA tissue as the tumor stage increases. The transcriptome analysis, GSEA analysis, Westernblot, and PCR results indicates TRPM2 is highly correlated with PKC/MAPK pathways.ConclusionTRPM2 could directly activate PKCα by calcium or indirectly activate PKCε and PKCδ by increased DAG in PC, which promote PC by downstream MAPK/MEK pathway activation.

scholarly journals Tumor Localization and Biodistribution with Radiolabeled Monoclonal Anbibody against Pancreatic Cancer in Tumor-Bearing Nude Mice.

1992 ◽  
Vol 168 (2) ◽  
pp. 397-401 ◽  
Author(s):  
YONG S. CHUNG ◽  
TETSUJI SAWADA ◽  
YASUYUKI KONDO ◽  
Jenny J.L. HO ◽  
YOUNG S. Kim ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Nude Mice ◽  
Tumor Localization ◽  
Tumor Bearing

Identification of gastrin as a growth peptide in human pancreatic cancer

1995 ◽  
Vol 268 (1) ◽  
pp. R135-R141 ◽  
Author(s):  
J. P. Smith ◽  
A. P. Fantaskey ◽  
G. Liu ◽  
I. S. Zagon
Keyword(s):  
Pancreatic Cancer ◽  
Receptor Antagonist ◽  
Nude Mice ◽  
Binding Capacity ◽  
Human Tumor ◽  
Human Pancreatic Cancer ◽  
Athymic Nude Mice ◽  
Cells In Culture ◽  
Pancreatic Cancer Cells ◽  
Regulation Of Growth

The present study reports the first evidence that the gastrointestinal peptide gastrin stimulates the growth of several human pancreatic cancer cells in culture and in tumors transplanted to nude mice. Gastrin promoted growth of all cell lines tested at a dose comparable to the binding affinity, providing evidence for a physiologically relevant receptor. The stimulatory effects of gastrin were blocked by the CCK-B/gastrin receptor antagonist L-365,260 and not by the CCK-A receptor antagonist L-364,718. Growth of PANC-1 cells in culture were inhibited by L-365,260, suggesting that gastrin is tonically produced by PANC-1 cells for regulation of growth. Athymic nude mice bearing PANC-1 xenografts were treated for 24 days subcutaneously with either 1% bovine serum albumin (diluent), pentagastrin (1 mg/kg), or L-365,260 (1 mg/kg) twice daily. Tumors from the pentagastrin-treated mice were found to weigh more and have greater protein and DNA content than controls, whereas these values were all decreased in tumors of L-365,260-treated mice. Receptor binding capacity changed in tumors of animals treated with the peptide or antagonist, suggesting a regulatory process. Gastrin immunoreactivity was detected in a transplanted PANC-1 human tumor. These results identify gastrin as a potent trophic peptide that actively stimulates growth of human pancreatic cancer and does so through a CCK-B/gastrin-like receptor.

scholarly journals TRPM2 promotes pancreatic cancer by PKC/MAPK pathway

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Rui Lin ◽  
Xunxia Bao ◽  
Hui Wang ◽  
Sibo Zhu ◽  
Zhongyan Liu ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Wound Healing ◽  
Western Blot ◽  
Nude Mice ◽  
Transcriptome Analysis ◽  
Cell Model ◽  
Mapk Pathway ◽  
Migration And Invasion ◽  
Tumor Bearing ◽  
Scratch Wound

AbstractThe mechanism of pancreatic cancer (PA) is not fully understanded. In our last report, TRPM2 plays a promising role in pancreatic cancer. However, the mechanism of TRPM2 is still unknown in this dismal disease. This study was designed to investigate the role and mechanism of TRPM2 in pancreatic cancer. TRPM2 overexpressed and siRNA plasmid were created and transfected with pancreatic cancer cell line (BxPC-3) to construct the cell model. We employed CCK-8, Transwell, scratch wound, and nude mice tumor-bearing model to investigate the role of TRPM2 in pancreatic cancer. Besides, we collected the clinical data, tumor tissue sample (TT) and para-tumor sample (TP) from the pancreatic cancer patients treated in our hospital. We analyzed the mechanism of TRPM2 in pancreatic cancer by transcriptome analysis, western blot, and PCR. We blocked the downstream PKC/MEK pathway of TRPM2 to investigate the mechanism of TRPM2 in pancreatic cancer by CCK8, scratch wound healing, and transwell assays. Overexpressed TRPM2 could promote pancreatic cancer in proliferation, migration, and invasion ability in no matter the cell model or nude mice tumor-bearing model. TRPM2 level is highly negative correlated to the overall survival and progression-free survival time in PA patients, however, it is significantly increased in PA tissue as the tumor stage increases. The transcriptome analysis, GSEA analysis, western-blot, and PCR results indicate TRPM2 is highly correlated with PKC/MAPK pathways. The experiments of PKC/MEK inhibitors added to TRPM2 overexpressed BxPC-3 cell showed that significant inhibition of PA cells happened in CCK8, transwell, and wound-healing assay. TRPM2 may directly activate PKCα by calcium or indirectly activate PKCε and PKCδ by increased DAG in PA, which promote PA by downstream MAPK/MEK pathway activation.

Efficacy of a repeat dose injection of CLR 131 (I-131-CLR1404) in U87-MG tumor-bearing athymic nude mice.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e14089-e14089
Author(s):  
Katherine Oliver ◽  
Jarrod Longcor ◽  
Irawati Kandela
Keyword(s):  
Therapeutic Effect ◽  
Nude Mice ◽  
Drug Formulation ◽  
External Beam Radiation ◽  
Repeat Dose ◽  
Post Injection ◽  
Beam Radiation ◽  
Athymic Nude Mice ◽  
Tumor Bearing ◽  
Significant Difference

e14089 Background: CLR 131 is a novel radioiodinated therapeutic that exploits the selective uptake and retention of phospholipid ethers (PLEs) by malignant cells. Study was to evaluate the therapeutic effect of CLR 131 when administered as a repeat dose intravenous injection in U87-MG tumor bearing mice. Malignant gliomas are associated with a low survival rate, and treatment with chemotherapy, external beam radiation, or surgery have shown limited effectiveness. In this study, therapeutic effect was evaluated by measurement of tumor volume increase and by survival of CLR 131 treated animals compared to animals dosed with a I-127-CLR1404 control. Methods: The U87-MG cell line (human glioma) was purchased from American Type Culture Collection (ATCC, Rockville, MD) and maintained in MEM media supplemented with 10% fetal bovine serum. Twenty female athymic nude mice (Harlan, Indianapolis, IN) weighing between 16-20 g were anaesthetized with isofluorene and inoculated subcutaneously in the right flank with 1 x 106 U87-MG tumor cells suspended in 50 μL PBS. The mice were given potassium iodide at a concentration of 0.1% in their drinking water to block possible free iodide in the drug formulation, with the addition of 0.4% (w/v) sweetener (Sweet and Low) to aid palatability, three days prior to injection and continuing through two weeks post injection. Doses of 95.7µCi and 109.0 µCi of CLR 131were given at Day 0 and Day 7 (N = 8 per group). Control doses of I-127-CLR1404 were given at the indicated dose and was injected via tail vein on Day 0 and Day 7. Results: The control group’s tumors increased 25-fold over a period of four weeks and the treatment group’s tumors increased 12-fold over the same period. There was a significant difference between the control and treatment tumor volumes. Mean survival time was 24.5 days and 31.9 days post injection, respectively. Conclusions: CLR 131 represents a unique, first in class targeted radiotherapeutic currently in clinical development. This study shows that CLR 131 dosed twice provides significant inhibition of tumor growth in a U87-MG tumor model which is known for very aggressive growth. Additionally, CLR 131 provided an overall survival benefit over the control group.

Ultrastructure of NPC/HK1 cells heterotransplanted in athymic nude mice

1982 ◽  
Vol 40 ◽  
pp. 236-237
Author(s):  
E.C. Chew ◽  
C.L. Li ◽  
D.P. Huang ◽  
H.C. Ho ◽  
L.S. Mak ◽  
...  
Keyword(s):  
Cell Line ◽  
Nude Mice ◽  
Biopsy Specimen ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Recurrent Tumour ◽  
Athymic Nude Mice ◽  
Cell Line Establishment ◽  
Fine Structures ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been established from a biopsy specimen of a recurrent tumour of the nasopharynx which was histologically diagnosed as a moderately to well differentiated squamous cell carcinoma. A definite decrease in the amount of tonofilaments and desmosomes in the NPC/HK1 cells during the cell line establishment was observed. The present communication reports on the fine structures of the NPC/HK1 cells heterotraneplanted in athymic nude mice.

972: Antisense CK2 Gene Therapy Induces Regression in Human Bladder Cancer Growing in Athymic Nude Mice

2004 ◽  
Vol 171 (4S) ◽  
pp. 257-258
Author(s):  
Joel Slaton ◽  
Daniel Sloper ◽  
Miriam Taylor ◽  
Alan Davis ◽  
Khalil Ahmed
Keyword(s):  
Gene Therapy ◽  
Bladder Cancer ◽  
Nude Mice ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Athymic Nude Mice

Integration of viral sequences into the c-myc gene in two mammary adenocarcinomas induced by polyomavirus in athymic nude mice.

1995 ◽  
Vol 69 (10) ◽  
pp. 5935-5945 ◽  
Author(s):  
M Berebbi ◽  
C Cajean-Feroldi ◽  
F Apiou ◽  
J Couturier ◽  
M Garcette ◽  
...  
Keyword(s):  
Nude Mice ◽  
Athymic Nude Mice ◽  
Myc Gene ◽  
Viral Sequences ◽  
Mammary Adenocarcinomas
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