Effect of the ratios of unsaturated fatty acids on the expressions of genes related to fat and protein in the bovine mammary epithelial cells

2015 ◽  
Vol 51 (4) ◽  
pp. 381-389 ◽  
Author(s):  
R. Sheng ◽  
S. M. Yan ◽  
L. Z. Qi ◽  
Y. L. Zhao
Keyword(s):  
Fatty Acids ◽  
Epithelial Cells ◽  
Unsaturated Fatty Acids ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells

Effects of exogenous C18 unsaturated fatty acids on milk lipid synthesis in bovine mammary epithelial cells

2020 ◽  
Vol 87 (3) ◽  
pp. 344-348
Author(s):  
Hang Zhang ◽  
Ni Dan ◽  
Changjin Ao ◽  
Sizhen Wang ◽  
Khas Erdene ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Epithelial Cells ◽  
Unsaturated Fatty Acids ◽  
Mammary Epithelial Cells ◽  
Lipid Synthesis ◽  
Mammary Epithelial ◽  
Control Treatment ◽  
Milk Lipid ◽  
Bovine Mammary Epithelial Cells

AbstractWe determined the effects of a combination of C18 unsaturated fatty acids (C18-UFAs) consisting of oleic, linoleic, and linolenic acids on milk lipogenesis in bovine mammary epithelial cells (BMECs). By orthogonal experiments to determine cellular triacylglycerol (TAG) accumulation, a combination of 200 μmol/l C18 : 1, 50 μmol/l C18 : 2, and 2 μmol/l C18 : 3 was selected as C18-UFAs combination treatment, and culture in medium containing fatty acid-free bovine serum albumin was used as the control. The expression of genes related to milk lipid synthesis and intracellular FA composition was measured. The results showed that cytosolic TAG formation was higher under C18-UFAs treatment than under control treatment. The mRNA expression of acetyl-CoA carboxylase-α (ACACA), fatty acid synthase (FASN), and peroxisome proliferator-activated receptor gamma (PPARG) did not differ between treatments. The abundance of stearoyl-CoA desaturase (SCD) and acyl-CoA synthetase long-chain family member 1 (ACSL1) was higher, whereas that of sterol regulatory element binding transcription factor 1 (SREBF-1) was lower after C18-UFAs treatment compared to control treatment. The C16 : 0 and SFA content was decreased following C18-UFAs treatment compared to control treatment, while the cis-9 C18 : 1 and UFA content was increased. In conclusion, C18-UFAs could stimulate triglyceride accumulation, increase the cellular UFA concentration, and regulate lipogenic genes in BMECs.

scholarly journals Effects of fatty acids on inducing endoplasmic reticulum stress in bovine mammary epithelial cells

2020 ◽  
Vol 103 (9) ◽  
pp. 8643-8654
Author(s):  
Mst Mamuna Sharmin ◽  
Moeko Mizusawa ◽  
Satoko Hayashi ◽  
Wataru Arai ◽  
Shotaro Sakata ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Endoplasmic Reticulum ◽  
Epithelial Cells ◽  
Endoplasmic Reticulum Stress ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells

scholarly journals β-Hydroxybutyrate Facilitates Fatty Acids Synthesis Mediated by Sterol Regulatory Element-Binding Protein1 in Bovine Mammary Epithelial Cells

2015 ◽  
Vol 37 (6) ◽  
pp. 2115-2124 ◽  
Author(s):  
Min Zhang ◽  
Shiqi Zhang ◽  
Qi Hui ◽  
Lin Lei ◽  
Xiliang Du ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Epithelial Cells ◽  
Fatty Acid Synthase ◽  
Mammary Epithelial Cells ◽  
Regulatory Element ◽  
Lipid Synthesis ◽  
Mammary Epithelial ◽  
Control Group ◽  
Bovine Mammary Epithelial Cells ◽  
Sterol Regulatory Element

Background/Aims: In dairy cows, β-hydroxybutyrate (BHBA) is utilized as precursors of de novo synthesized fatty acids in mammary gland. Ketotic cows are characterized by excessive negative energy balance (NEB), which can further increase the blood BHBA concentration. Sterol regulatory element-binding protein1 (SREBP1) and cell death-inducing DNA fragmentation factor-alpha-like effector α (Cidea) play crucial roles in lipid synthesis. Therefore, we hypothesized that BHBA could stimulate SREBP1/Cidea pathway to increase milk fat synthesis in bovine mammary epithelial cells. Methods: Bovine mammary epithelial cells were treated with different concentrations of BHBA and transfected with adenovirus to silence SREBP1 expression. The effects of BHBA on the lipid synthesis in bovine mammary epithelial cells were investigated. Results: The results showed that BHBA could significantly increase the expression of SREBP1, fatty acid synthase (FAS), acetyl-CoA carboxylase α (ACC-α), Cidea and diacylglycerol transferase-1 (DGAT-1), as well as the triglycerides (TG) content in bovine mammary epithelial cells. BHBA treatment also increased the transfer of mature SREBP1 to nucleus compared with control group. However, SREBP1 silencing could significantly down-regulate the overexpression of FAS, ACC-α, Cidea and DGAT-1, as well as TG content induced by BHBA. Conclusion: The present data indicate that BHBA can significantly increase TG secretion mediated by SREBP1 in bovine mammary epithelial cells.

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