Induction and early identification of tetraploid black locust by hypocotyl in vitro

The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type ?Velebit? from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 ?M of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole- 3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 ?M of IBA. However, treatment with 10 ?M of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings.

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CORRELATION OF CLINICAL PROGRESSION IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CHILDREN WITH IN VITRO ZIDOVUDINE RESISTANCE BY A DIRECT QUANTITATIVE PERIPHERAL BLOOD LYMPHOCYTE ASSAY

PEDIATRICS ◽

10.1542/peds.98.2.346 ◽

1996 ◽

Vol 98 (2) ◽

pp. 346-346

Author(s):

Jan P. Sinclair ◽

I. Celine Hanson

Keyword(s):

Human Immunodeficiency Virus ◽

Antiretroviral Therapy ◽

Early Identification ◽

Blood Lymphocyte ◽

Clinical Progression ◽

Current Standard ◽

Enhanced Sensitivity ◽

Immunodeficiency Virus ◽

Azt Resistance

The DQPBL assay has a number of advantages over the consensus ACTG assay, being faster and less costly. The reliability of the DQPBL in comparison to the current standard is demonstrated (67% concordance, 29% enhanced sensitivity and 5% discordance). In this study, there was a correlation between AZT resistance and disease progression requiring change in antiretroviral therapy. Whether early identification of resistance to AZT will enable timely change in antiretroviral therapy, and slowing of clinical and immunologic deterioration remains to be demonstrated.

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Experimental research on cutting force and cutting parameters of Tetraploid Black Locust

2011 International Conference on New Technology of Agricultural ◽

10.1109/icae.2011.5943770 ◽

2011 ◽

Author(s):

Yuan xiangyue ◽

Chen Zhongjia

Keyword(s):

Cutting Force ◽

Experimental Research ◽

Black Locust ◽

Cutting Parameters ◽

Tetraploid Black Locust

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Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage

Ciência Rural ◽

10.1590/0103-8478cr20180427 ◽

2019 ◽

Vol 49 (7) ◽

Author(s):

Zhihao Dong ◽

Junfeng Li ◽

Lei Chen ◽

Siran Wang ◽

Tao Shao

Keyword(s):

Lactic Acid ◽

Chemical Composition ◽

Lactobacillus Plantarum ◽

Dry Matter ◽

Neutral Detergent Fiber ◽

In Vitro Digestibility ◽

Fermentation Products ◽

Fermentation Quality ◽

Tetraploid Black Locust

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.

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Differential Tolerance of Woody Nursery Crop Seedlings to Napropamide

Weed Technology ◽

10.1017/s0890037x00032851 ◽

1989 ◽

Vol 3 (4) ◽

pp. 584-589 ◽

Cited By ~ 1

Author(s):

Sumaryono ◽

Garvin Crabtree

Keyword(s):

Ponderosa Pine ◽

Shoot Growth ◽

Black Locust ◽

Soil Layer ◽

Woody Species ◽

Herbicide Application ◽

Deciduous Species ◽

Coniferous Species ◽

Secondary Roots

Experiments are conducted to evaluate the tolerance of three deciduous species: black locust, honeylocust, apple, and three coniferous species: Douglas fir, ponderosa pine, and Japanese black pine, in the seedling stage to napropamide. Deciduous species were more susceptible to napropamide than coniferous species. Domestic apple had the slowest seed germination and root development and was more susceptible. The deciduous species had more secondary roots in the shallow soil layer which contained most of the herbicide than the coniferous species. The roots of all woody species tested in vitro were inhibited significantly by contact with the herbicide, but only shoot growth of domestic apple and black locust was inhibited. Injury to woody nursery crop seedlings may be avoided by delaying herbicide application.

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