scholarly journals Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage

2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Zhihao Dong ◽  
Junfeng Li ◽  
Lei Chen ◽  
Siran Wang ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
Fermentation Quality ◽  
Tetraploid Black Locust

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.

Fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages prepared with whole-plant corn (Zea mays L.) and hulless barley (Hordeum vulgare L.) straw

2018 ◽  
Vol 58 (10) ◽  
pp. 1860 ◽  
Author(s):  
XianJun Yuan ◽  
AiYou Wen ◽  
Jian Wang ◽  
JunFeng Li ◽  
Seare T. Desta ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Ammonia Nitrogen ◽  
Stability Test ◽  
Small Scale ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was carried out to assess the effects of adding Lactobacillus plantarum, molasses or/and ethanol on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration (TMR) silage, which is well accepted in small-scale dairy farms in Tibet. Total mixed ration were ensiled in laboratory silos (1 L) and treated with (1) no additive (Control), (2) ethanol (E, 25 ml/kg fresh weight (FW)), (3) molasses (M, 30 g/kg FW); (4) Lactobacillus plantarum (L, 106cfu/g FW); (5) ethanol + molasses (EM); and (6) ethanol + Lactobacillus plantarum (EL). After 45 days of ensiling, six silos per treatment were opened for the fermentation quality and in vitro digestibility analyses, whereas 18 silos were used for the aerobic stability test for the following 9 days. All TMR silages were well preserved with dominant lactic acid (LA), low pH and ammonia nitrogen, and negligible propionic and butyric acid. The L and EL silages had the lowest pH and highest LA concentrations. The addition of ethanol did not inhibit silage fermentation as there were no significant differences for the pH, LA, acetic acid, negligible propionic acid or ammonia nitrogen content, lactic acid bacteria and yeast counts between Control and the E silage. During the aerobic stability test, pH increased by 1.39, 1.67, 1.69 and 0.74 for the Control, M, L and EM silages, but only 0.40 and 0.34 for E and EL silages, respectively. Upon exposure to air, the LA concentration in the L silage was evidently (P < 0.05) decreased, whereas LA concentration in the EL silage remained the highest value after the third day of aerobic exposure. Mean populations of aerobic bacteria and yeast in the E and EL silages were lower (P < 0.05) than those of the Control. These findings suggested that L. plantarum is effective in improving fermentation quality of TMR silages. Although the addition of ethanol in our study did not depress the fermentation of the TMR silages, it showed potential to inhibit the aerobic spoilage of TMR silages, either alone or in combination with the L. plantarum. It is concluded that L. plantarum combined with ethanol not only ensures better fermentation but also could improve aerobic stability.

scholarly journals Assessing the nutritional value of agroindustrial co-products and feed through chemical composition, in vitro digestibility, and gas production technique

2017 ◽  
Vol 39 (3) ◽  
pp. 289 ◽  
Author(s):  
Paula Martins Olivo ◽  
Geraldo Tadeu dos Santos ◽  
Luís Carlos Vinhas Ítavo ◽  
Ranulfo Combuca da Silva Junior ◽  
Eduardo Souza Leal ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Soybean Hulls ◽  
Citrus Pulp

Agroindustrial co-products are a viable alternative for use in animal nutrition. Tests were conducted using eight different types of co-products and feed to evaluate the chemical composition, in vitro digestibility of dry matter, crude protein and neutral detergent fiber, and gas production by them. The co-products tested were: coffee hulls; pelleted citrus pulp; grape residue; soybean hulls; cottonseed; cassava foliage; and foods usually supplied to ruminants: corn silage and ground corn concentrate. Data of in vitro digestibility of dry matter, crude protein and neutral detergent fiber were tested by analysis of variance using the least square method; the results of gas production were interpreted by a non-linear regression by the Gauss-Newton method; and the effects of treatments were evaluated by the Tukey’s test. The coefficients of in vitro digestibility of dry matter, crude protein and neutral detergent fiber of co-products were different. Gas production was also different between co-products and feeds evaluated for the volume of gas produced from the fast and slow degradation fractions, degradation rate, bacterial colonization time, and the total volume of gas produced. The evaluated co-products exhibited greater in vitro dry matter digestibility compared to corn silage, except for cottonseed, grape residue, and cassava foliage. Co-products showed higher values of in vitro crude protein digestibility compared to corn silage, and a reduced in vitro digestibility of neutral detergent fiber, except for pelleted citrus pulp and soybean hulls. Corn silage produced larger volume of gas from the fast degradation fraction compared to the co-products and corn concentrate. Co-products analyzed had appropriate nutritional characteristics according to the techniques applied and can be included in ruminant diets. 

scholarly journals Bromatological and microbiological characteristics of sugarcane silages treated with calcium oxide

2010 ◽  
Vol 39 (7) ◽  
pp. 1398-1408 ◽  
Author(s):  
Jucilene Cavali ◽  
Odilon Gomes Pereira ◽  
Sebastião de Campos Valadares Filho ◽  
Edson Mauro Santos ◽  
Gleidson Giordano Pinto de Carvalho ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Calcium Oxide ◽  
Sugar Cane ◽  
Dry Matter ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Quadratic Models

It was evaluated the addition of calcium oxide (0; 0.5; 1.0; 1.5; and 2.0% of dry matter) effect on the chemical composition and ruminal degradability of the dry matter and neutral detergent fiber, on the losses of dry matter, and on the microbial population in sugar cane silages. A design with five levels of calcium oxide and three replications was used. All the variables were influenced by the addition of calcium oxide in the silages, except the composition in acid detergent insoluble protein, water soluble carbohydrates and lignin. The dry matter content and pH linearly increased while the contents of organic matter, hemicellulose and crude protein linearly decreased with the levels of calcium oxide. Neutral detergent fiber corrected for ashes and protein and acid detergent fiber were adjusted for the quadratic models with calculated minimal values of 33.3 and 22.5% for the levels 1.73 and 1.49% of calcium oxide, respectively. The in vitro digestibility of the dry matter and the content of amoniacal nitrogen adjusted to quadratic models with maximal levels of 80.1% and 9.1% for the levels of 1.8 and 0.7% of calcium oxide, respectively. The minimal production of gas (3.18%) was observed at the level of 1.39% of calcium oxide. Production of effluent and recovery of dry matter of the ensiled mass showed a reduction and a linear increase, respectively, with addition of calcium oxide. The highest lactic acid bacterial population was observed in the silage treated with 1.5% of calcium oxide. The addition of calcium oxide increased degradability of the soluble fraction of the dry matter, which was higher than 50% and reduced the non- degradable fraction of the neutral detergent fiber. Addition of calcium oxide level higher than 1.0% in the sugar cane during ensilage improves in vitro dry matter digestibility and the recovery of dry matter, increases populations of lactic acid bacteria and reduces production of yeasts.

scholarly journals Chemical composition and dry matter digestibility of sugar cane oxide treated with calcium

2014 ◽  
Vol 66 (2) ◽  
pp. 529-538 ◽  
Author(s):  
C.O. Romão ◽  
G.G.P. Carvalho ◽  
V.M. Leite ◽  
A.S. Santos ◽  
D.M.T. Chagas ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Sugar Cane ◽  
Dry Matter ◽  
Calcium Content ◽  
Neutral Detergent Fiber ◽  
Mineral Matter ◽  
Phosphorus Concentration ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility

The aim of this study was to identify the most adequate level of calcium oxide (CaO) in the treatment of sugar cane by evaluating the chemical composition and in vitro digestibility of dry matter. The sugar cane was homogenized with CaO levels 0, 0.75, 1.5, 2.25, 3.0, 3.75 and 4.5%, in natura matter, for 24 hours. The dry matter and mineral matter increased, while the organic matter of the sugar cane decreased (P <0.05) linearly as a result of the CaO levels. The CaO had no effect on the levels of crude protein and ether extract. The phosphorus concentration remained unchanged, but there was a significant increase (P<0.05) in the calcium content. The contents of neutral detergent fiber, acid detergent fiber and cellulose decreased linearly with the levels of CaO. The average levels of indigestible dry matter and indigestible neutral detergent fiber significantly decreased with increasing levels of CaO. The results showed that the in vitro dry matter digestibility (IVDMD) was different between fresh sugar cane and sugar cane with added levels of CaO from 1.5%. The IVDMD and nutritional value of the sugar cane was improved by adding 1.5% CaO based on fresh content.

scholarly journals Effects of Adding Pre-Fermented Fluid Prepared from Red Clover or Lucerne on Fermentation Quality and in Vitro Digestibility of Red Clover and Lucerne Silages

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 454
Author(s):  
Lin Sun ◽  
Yun Jiang ◽  
Qinyin Ling ◽  
Na Na ◽  
Haiwen Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Red Clover ◽  
Ammonia Nitrogen ◽  
In Vitro Digestibility ◽  
Fermentation Quality ◽  
Fermentation Parameters ◽  
Positive Effect

This study examined the effects of chopping or chopping + blender maceration of red clover or lucerne on pre-fermented juice (PFJ) and determined the effects of PFJs on the quality of red clover silage or lucerne silage. The PFJs from chopping red clover (PFJ-RC) or lucerne (PFJ-LC) had a higher lactic acid bacteria (LAB) count than that from chopping + blender maceration (p < 0.05) and were used as additives. Compared with the Control of both silages, adding PFJ increased LAB, lactic acid (LA), and in vitro digestibility of dry matter (IVDMD) (p < 0.05), while pH, acetic acid (AA), and ammonia nitrogen/total nitrogen (NH3-N/TN) were decreased (p < 0.05). For red clover silages, the PFJ-RC treatment contained the greatest LAB and LA and the lowest pH and NH3-N/TN among treatments (p < 0.05); similar results were observed in PFJ-LC treatment for lucerne silages (p < 0.05). The IVDMD of both silages correlated negatively with pH, AA, and NH3-N/TN and positively with LA (p < 0.05). Overall, chopping alone was a better method for preparing PFJ. Adding PFJ at ensiling increased LA and decreased the pH, AA, and NH3-N/TN of both silages. Ensiling lucerne or red clover with PFJ from the ensiling material had a more positive effect on the fermentation parameters mentioned above. Satisfactory fermentation parameters detected in the present study contributed to improving the IVDMD of both silages.

scholarly journals Influence of the period of year on the chemical composition and digestibility of pasture and fodder selected by goats in caatinga

2017 ◽  
Vol 18 (3) ◽  
pp. 402-416 ◽  
Author(s):  
Messias José dos Santos SILVA ◽  
Dulciene Karla de Andrade SILVA ◽  
André Luiz Rodrigues MAGALHÃES ◽  
Kedes Paulo PEREIRA ◽  
Érica Carla Lopes da SILVA ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Semiarid Region ◽  
Mineral Matter ◽  
In Vitro Digestibility ◽  
Randomized Design ◽  
Total Carbohydrates ◽  
Completely Randomized Design

SUMMARY This study aimed to verify the influence of months on chemical composition and in vitro digestibility of pasture and forage selected by goats in the semiarid region of Brazil’s Pernambuco state. Six male goats fistulated in the rumen were used in a completely randomized design. Forage collection was conducted in herbaceous and shrub/tree layers, in order to simulate goat grazing, and materials were separated into leaves and branches. Extrusa and pasture collections were held in November, January and February/March. There was collection month (P <0.05) effect for pasture on dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), with average values of 458.27g/kg NM and 155.46, 521.30 and 390.13g/kg DM, respectively. For the stem (P <0.05) for DM, Organic matter (OM), mineral matter (MM), ethereal stratum (EE), ADF, acid detergent unavailable protein (AIP), total carbohydrates (TCHO) and dry matter in vitro digestibility (DMIVD), with average values of 487.47g/kg NM and 930.80, 69.07, 20.67, 440.30 and 63.78g/kg DM, respectively. About the extrusa, there was collection time effect (P <0.05) on the DM, OM, MM, CP, EE, AIP, TCHO, Non-fibrous carbohydrates (NFC) and DMIVD, with average values of 84.53g/kg DM, 863.03, 136.97, 152.90, 33.60, 14.07, 676.37 and 55.35g/kg DM, respectively. Rainfall changes in the rainy season in the caatinga altered pasture quality, causing changes in the diet selected by goats, mainly in DM, CP, CNF and DMIVD.

scholarly journals The Impact of Ammoniation Treatment on the Chemical Composition and In Vitro Digestibility of Rice Straw in Chinese Holsteins

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1854
Author(s):  
Yulin Ma ◽  
Xu Chen ◽  
Muhammad Zahoor Khan ◽  
Jianxin Xiao ◽  
Shuai Liu ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutritive Value ◽  
Corn Steep Liquor ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
The Impact

The current study was conducted to explore the ammoniation treatment effects on the chemical composition and in vitro digestibility of rice straw in Chinese Holsteins. For this purpose, rice straw was stored in polyethylene bags (35 × 25 cm, 350 g per bag) including (i) no additives (RS); (ii) 5% urea (5U, dry matter (DM) basis); (iii) 9% corn steep liquor + 5% urea (9C5U, DM basis); (iv) 9C2.5U; and (v) 9C2.5U + 3% molasses (9C2.5U3M, DM basis). The air-dry matter of the mixture was kept at the same level at 55% for all treatments. Fifteen bags (5 treatments × 3 repeats) were prepared and stored at ambient temperature (25 ± 3 °C). The chemical composition and in vitro digestibility were measured at day 60 after storage. Our analysis revealed that all the four ammoniation treatments improved the in vitro DM and neutral detergent fiber (IVNDFD) digestibility. In addition, all the four ammoniation treatments significantly (P < 0.001) increased the levels of crude protein (CP), gas production (GP), acetic acid (AA), butyric acid (BA) and total volatile fatty acid (TVFA) contents of the rice straw and decreased the neutral detergent fiber (NDF) and acid detergent fiber (ADF) of the rice straw compared to the control. Within four treated groups, the 9C5U treatment was most effective. Finally, we concluded that ammoniation treatments increased the nutritive value of rice straw. In addition the 9C5U treatment could be an effective ammoniation treatment for the better utilization of rice straw.

scholarly journals Nutritional evaluation of Brachiaria brizantha cv. Marandu cultivated in soils developed from basalt and sandstone in the state of Paraná

2019 ◽  
Vol 40 (1) ◽  
pp. 469
Author(s):  
Geisi Loures Guerra ◽  
Thierry Becquer ◽  
Pedro Rodolfo Siqueira Vendrame ◽  
Sandra Galbeiro ◽  
Osmar Rodrigues Brito ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Nutritional Composition ◽  
Soil Samples ◽  
The State ◽  
Neutral Detergent Fiber ◽  
Mineral Matter ◽  
In Vitro Digestibility ◽  
Brachiaria Brizantha

Determining the nutritional composition of a pasture is necessary to evaluate its quality, aiming to meet the requirements of feeding animals and guarantee good performance. The quality of the forage biomass produced depends directly on the fertility and type of soil, environmental conditions, and management. In this scenario, the aim was to evaluate the chemical composition, in vitro digestibility of dry matter, and macro- and micronutrient contents of Brachiaria brizantha cv. Marandu cultivated in soils developed from basalt and sandstone in the state of Paraná. A total of 120 forage samples were collected in a soil developed from basalt and 116 in soil developed from sandstone. Forage harvesting occurred in autumn and spring for basalt soil (season effect), and in spring (soil effect) for sandstone soil. Soil samples (0–20 cm) were collected in the spring season, at the same forage collection sites, to obtain a greater sample representativity regarding climate–soil–plant relationships. Forage samples were separated in leaf blade and stem + sheath, and analyses of dry matter (DM), mineral matter (MM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, cellulose, hemicellulose, in vitro dry matter digestibility (IVDMD), total digestible nutrients (TDN), nitrogen, and macro- and micronutrient contents were performed. In soil samples, the contents of P, K, Ca, Mg, Mn, and Zn were analyzed. There were season and soil-type effects on the chemical composition of the cultivar studied. The highest levels of protein, NDF, ADF, lignin, and cellulose were obtained in autumn. In samples from soil developed from basalt, higher levels of hemicellulose, IVDMD, and TDN were obtained in the spring. K, P, Mn, and Zn presented levels within the range recommended for the studied forage, but the values of Ca and Mg in autumn and N in the three studied periods remained below the level considered adequate. Soil nutrient contents were influenced (P < 0.05) by the soil source material, and the highest levels of K, Ca, Mg, Mn, and Zn were observed in soil developed from basalt. Forage cultivated in spring in the soil area developed from basalt, was nutritionally superior in relation to forage cultivated in the soil developed from sandstone.

scholarly journals Effects of additives on the fermentation quality, in vitro digestibility and aerobic stability of mulberry (Morus alba L.) leaves silage

2020 ◽  
Vol 33 (8) ◽  
pp. 1292-1300
Author(s):  
Zhihao Dong ◽  
Siran Wang ◽  
Jie Zhao ◽  
Junfeng Li ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Fresh Matter ◽  
Mulberry Leaves ◽  
Aerobic Stability ◽  
Fermentation Quality

Objective: To explore feed resources capable of replacing regular poor-quality fodder, this study was conducted to evaluate the effects of additives on the fermentation quality, <i>in vitro</i> digestibility and aerobic stability of mulberry leaves silage.Methods: The mulberry leaves were ensiled either untreated (control) or treated with 1×10<sup>6</sup> cfu/g fresh matter <i>Lactobacillus plantarum</i> (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and <i>Lactobacillus plantarum</i> (L+G), and a combination of 3% molasses and <i>Lactobacillus plantarum</i> (L+M). The fermentation quality and chemical composition were analyzed after 7, 14, 30, and 60 d, respectively. The 60-d silages were subjected to an aerobic stability test and fermented with buffered rumen fluid to measure the digestibility.Results: Inoculating lactic acid bacteria (LAB) resulted in more rapid increase in lactic acid concentrations and decline in pH of mulberry leaves silage as compared control. Higher acetic acid and lower ethanol and ammonia nitrogen concentrations (p<0.05) were observed in the LAB-inoculated silages as opposed to control during ensiling. The LAB-inoculated silages contained lower water-soluble carbohydrates compared with control during the first 14 d of ensiling, and lower neutral detergent fibre (p<0.05) concentrations as compared with non-LAB inoculated silages. Adding molasses alone increased (p<0.05) the digestibility of dry matter (DM). The aerobic stability of mulberry leaves silage was increased by LAB inoculation, whereas decreased by adding glucose or molasses.Conclusion: The LAB inoculation improved fermentation quality and aerobic stability of mulberry leaves silage, while adding glucose or molasses failed to affect the fermentation and impaired the aerobic stability. Inoculating LAB alone is recommended for mulberry leaves especially when ensiled at a relatively high DM.

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