Purification, chemical characterization and antioxidant activities of polysaccharides of fruiting bodies of Pleurotus ferulae mushroom

Abstract Hymenocrater longiflorus was collected from northern Iraq, and the chemical composition and antioxidant and cytotoxic activities of this plant were investigated. Ten compounds detected by HPLC-ESI/MS were identified as flavonoids and phenolic acids. The free radical scavenging activity of the 70% methanol extract was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The antioxidant activities of the extract may be attributed to its polyphenolic composition. The cytotoxicity of the plant extract against the osteosarcoma (U2OS) cell line was assessed with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The extract significantly reduced the viability of cells in a concentration- and time-dependent manner. Cells were arrested during the S-phase of the cell cycle, and DNA damage was revealed by antibodies against histone H2AX. The apoptotic features of cell shrinkage and decrease in cell size were also observed. Western blot analysis revealed cleavage of poly (ADP-ribose)-polymerase 1 (PARP-1), in addition to increases in the proteins p53, p21, and γ-H2AX. Collectively, our findings demonstrate that the H. longiflorus extract is highly cytotoxic to U2OS cells, most likely due to its polyphenolic composition.

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Hepatoprotective and Antioxidant Activities of Oil from Baru Almonds (Dipteryx alata Vog.) in a Preclinical Model of Lipotoxicity and Dyslipidemia

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/8376081 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 4

Author(s):

Mayara Áthina Reis ◽

Rômulo Dias Novaes ◽

Sueli Regina Baggio ◽

André Luiz Machado Viana ◽

Bruno Cesar Correa Salles ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activities ◽

Liver Steatosis ◽

Chemical Characterization ◽

High Energy ◽

Preclinical Model ◽

Histopathological Analysis ◽

Popular Medicine ◽

Male Wistar Rats ◽

Dipteryx Alata

The oil obtained from baru (Dipteryx alata Vog.) almonds exhibits high energy value and is reported in popular medicine for the treatment of rheumatic diseases and reproductive disturbances. Although baru oil is used in domestic cuisine, the chemical characterization of this oil and its effects on lipid metabolism are still poorly understood. Therefore, this study evaluated the fatty acid (FA) profile and the effects of baru oil on liver and aorta in a murine model of dyslipidemia. The chromatographic profile of baru oil showed high levels of unsaturated FAs, especially oleic acid. Saturated FAs, such as palmitic and lignoceric acids, were found in lower amounts. Hypercholesterolemia was induced in male Wistar rats by daily administration of a lipid emulsion by gavage for 15 weeks. Biochemical and histopathological analysis were performed on serum, aorta, and liver. The results demonstrated that animals developed marked hypercholesterolemia, liver steatosis, and increased lipid peroxidation in the aorta. Treatment with baru oil attenuated lipid peroxidation and drastically reduced liver damage, especially ballooning degeneration and steatosis. By restricting vascular and hepatic injury, this oil showed potential applicability as a functional food, reinforcing its use in popular medicine and domestic cuisine.

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Chemical characterization and antioxidant activities of Morus alba var. nigra fruits

Scientia Horticulturae ◽

10.1016/j.scienta.2019.04.040 ◽

2019 ◽

Vol 253 ◽

pp. 120-127 ◽

Cited By ~ 2

Author(s):

Maryam Farahani ◽

Hossein Salehi-Arjmand ◽

Ali Khadivi ◽

Morteza Akramian

Keyword(s):

Antioxidant Activities ◽

Chemical Characterization ◽

Morus Alba

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Chemical characterization, antiproliferative and antiadhesive properties of polysaccharides extracted from Pleurotus pulmonarius mycelium and fruiting bodies

Applied Microbiology and Biotechnology ◽

10.1007/s00253-009-2296-x ◽

2009 ◽

Vol 85 (6) ◽

pp. 1977-1990 ◽

Cited By ~ 40

Author(s):

Iris Lavi ◽

Dana Levinson ◽

Irena Peri ◽

Yoram Tekoah ◽

Yitzhak Hadar ◽

...

Keyword(s):

Chemical Characterization ◽

Fruiting Bodies ◽

Pleurotus Pulmonarius

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The Study of Steaming Durations and Temperatures on the Chemical Characterization, Neuroprotective, and Antioxidant Activities of Panax notoginseng

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2022/3698518 ◽

2022 ◽

Vol 2022 ◽

pp. 1-13

Author(s):

Didi Ma ◽

Jue Wang ◽

Guo Yin ◽

Lijun Wang ◽

Yibao Jin ◽

...

Keyword(s):

Antioxidant Activities ◽

Chemical Characterization ◽

Panax Notoginseng ◽

Absorption Capacity ◽

Traditional Chinese Medicines ◽

Chinese Medicines ◽

Hplc Fingerprint ◽

Relationship Analysis ◽

Antioxidant Effects ◽

Spectrum Effect

Panax notoginseng (PN) is one of the most valuable traditional Chinese medicines and has extensive pharmacological effects. Recent studies demonstrated that PN exhibited pharmacological effect related to Alzheimer’s disease (AD). However, whether steaming process can boost its anti-AD activity is still unexplored. To fill this gap, effects of steaming durations and temperatures on the chemical characterization, neuroprotective and antioxidant activities of PN were systematically investigated in this study. HPLC fingerprint coupled with quantitative analysis demonstrated striking conversion of original saponins to less polar ones with the increase in the steaming time and temperature. In the viewpoint of anti-AD activity on neuroprotective and antioxidant effects, several steamed PN samples (110°C-6/8/10 h, 120°C ‐4/6 h samples) displayed a significant increase both in cell viability and oxygen radical absorption capacity (ORAC) values compared with the no steamed one ( P < 0.01 or P < 0.005 ). Steaming temperature had the greater impact on the change of chemical composition and anti-AD activity of PN. Moreover, the spectrum-effect relationship analysis revealed that the transformed saponins were partially responsible for the increased neuroprotective and antioxidant effects of steamed PN. Therefore, steamed PN could be used as a potential crude drug for prevention and treatment of AD.

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