Optimization of extraction of bioactive phenolics and their antioxidant potential from callus and leaf extracts of Stevia rebaudiana Bertoni

2021 ◽  
Author(s):  
Ramandeep Kaur ◽  
Pooja Manchanda ◽  
Gurupkar Singh Sidhu
Keyword(s):  
Stevia Rebaudiana ◽  
Antioxidant Potential ◽  
Leaf Extracts ◽  
Stevia Rebaudiana Bertoni

scholarly journals Stevia rebaudiana Bertoni cultivated in Portugal: A prospective study of its antioxidant potential in different conservation conditions

2016 ◽  
Vol 90 ◽  
pp. 49-55 ◽  
Author(s):  
Marisa Barroso ◽  
Lillian Barros ◽  
M. Ângelo Rodrigues ◽  
Maria João Sousa ◽  
Celestino Santos-Buelga ◽  
...  
Keyword(s):  
Prospective Study ◽  
Stevia Rebaudiana ◽  
Antioxidant Potential ◽  
Stevia Rebaudiana Bertoni ◽  
A Prospective Study

scholarly journals STEVIA (STEVIA REBAUDIANA BERTONI): BIOCHEMICAL COMPOSITION, THERAPEUTIC PROPERTIES AND USE IN THE FOOD INDUSTRY (REVIEW)

2021 ◽  
pp. 5-27
Author(s):  
Aleksey Aleksandrovich Kochetov ◽  
Nadezhda Georgiyevna Sinyavina
Keyword(s):  
Biochemical Composition ◽  
Food Industry ◽  
Antioxidant Properties ◽  
Stevia Rebaudiana ◽  
Mineral Elements ◽  
Leaf Extracts ◽  
Low Calorie ◽  
Stevia Rebaudiana Bertoni ◽  
Number Of Patients ◽  
Therapeutic Properties

The review presents the current state of the science for stevia (Stevia rebaudiana Bertony), its biochemical composition, therapeutic properties and using in the food industry. Interest in this plant, a source of natural low-calorie sweeteners, is associated with increased consumption of dietary sweeteners, caused by an increase in the number of patients with diabetes, metabolic syndrome, and with many negative effects from the use of synthetic sweeteners. Diterpene glycosides from stevia have a high sweetness (50-350 times sweeter than sucrose), low calorie content, safe for human health. In addition to them, a number of biologically valuable compounds (mineral elements, vitamins, proteins, amino acids, oligosugars, fiber, polyphenols) were found in the leaves of stevia. High antioxidant properties of stevia leaf extracts has been shown, which allows them to be used in the food industry in the production of functional products. Numerous data have been obtained on the various therapeutic properties of stevia. It has been revealed that regular use of stevia glycosides reduces blood glucose and cholesterol levels, promotes cell regeneration, inhibits the growth of tumor cells and strengthens blood vessels. Components from stevia leaves have a choleretic, diuretic effect, normalize blood pressure, prevent diseases of the gastrointestinal tract, and have a beneficial effect on kidney function. Stevia extracts also have antibacterial, antiviral, anti-inflammatory and immunomodulatory effects. The safety and stability of stevia sweet glycosides in an acidic environment and at high temperatures allows them to be widely used in the production of products such as diet drinks, teas, sauces, marinades, seafood, ice cream, yogurts, pastries, culinary products and sweets.

Antioxidant potential of Stevia rebaudiana (Bertoni)

Pathology ◽  
2020 ◽  
pp. 345-356
Author(s):  
Kashif Ameer ◽  
Gui-Hun Jiang ◽  
Rai Muhammad Amir ◽  
Jong-Bang Eun
Keyword(s):  
Stevia Rebaudiana ◽  
Antioxidant Potential ◽  
Stevia Rebaudiana Bertoni

Elevated antioxidant potential of chlorocholine chloride-treated in vitro grown Stevia rebaudiana Bertoni

2013 ◽  
Vol 35 (6) ◽  
pp. 1775-1783 ◽  
Author(s):  
Avishek Dey ◽  
Souren Paul ◽  
Sayanti Kundu ◽  
Abhijit Bandyopadhyay ◽  
Aloke Bhattacharjee
Keyword(s):  
Stevia Rebaudiana ◽  
Antioxidant Potential ◽  
Chlorocholine Chloride ◽  
Stevia Rebaudiana Bertoni

scholarly journals Antimicrobial and Antioxidant Potential of Methanolic Extracts from Different Parts of Stevia rebaudiana Bertoni Cultivated in Bulgaria

2021 ◽  
Vol 50 (9) ◽  
pp. 2641-2651
Author(s):  
Toncho Dinev ◽  
Milena Tzanova ◽  
Nikolina Rusenova ◽  
Neli Grozeva ◽  
Mariya Gerdzhikova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
The Other ◽  
Antioxidant Potential ◽  
Diffusion Method ◽  
Stevia Rebaudiana Bertoni ◽  
Methanolic Extracts ◽  
Different Parts

Stevia rebaudiana Bertoni is a plant species which is frequently used not only as a sweetener, but also for its antibacterial and antioxidant properties. Nowadays, there are a large number of studies on the antimicrobial and antioxidant activity of S. rebaudiana leaves, but there are almost no data about the antimicrobial and antioxidant potential of extracts from the other parts of S. rebaudiana. The aim of the present study is to provide data of the antibacterial and antioxidant potential of methanolic extracts from different parts of S. rebaudiana (flowers, leaves, stems, rhizomes, and tubers) cultivated in Bulgaria. Antibacterial activity of the extracts against Staphylococcus aureus, Bacillus cereus and Escherichia coli was evaluated by agar well diffusion method, rutin content - by HPLC method, total phenolic content and radical scavenging potential - by UV-Vis analysis. S. rebaudiana extracts demonstrated antibacterial activity mainly against S. aureus - flower extracts expressed the highest activity, followed by the leaf and stem extracts. Only flower and leaf extracts demonstrated very low antibacterial activity against B. cereus. S. rebaudiana extracts did not show any antibacterial activity against E. coli. Methanolic extracts of this plant are rich in antioxidants. The highest concentrations of rutin and total phenols were found in the rhizomes of the plants, followed by the leaves, tubers, flowers, and stems, which corresponded to the radical scavenging potential of the same plant part. Comparisons between Trolox equivalents and gallic acid equivalents in different parts of S. rebaudiana on one hand, and Trolox equivalents and rutin concentration on the other hand showed a positive dependence and high values of the Pearson correlation - 0.9612 and 0.9707, respectively. The most important part of S. rebaudiana with medicinal significance (the leaves) has both comparatively good antibacterial activity and high antioxidant content, although the flowers and rhizomes expressed higher antibacterial and antioxidant activity, respectively. The experimental results imply that the cultivation area and climatic conditions of Bulgaria are very suitable for cultivation of S. rebaudiana plants with high content of antioxidants.

scholarly journals Antibiofilm and antimicrobial activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria

2019 ◽  
Vol 1 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Aerma Hastuty
Keyword(s):  
Drug Resistance ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Stevia Rebaudiana ◽  
Antimicrobial Activities ◽  
Structural Form ◽  
Leaf Extracts ◽  
Stevia Rebaudiana Bertoni ◽  
Microbial Group ◽  
Antimicrobial Assay

Biofilm is a structural form of a microbial group that is protected by the Extracellular Polymeric Substance (EPS) matrix. The biofilm is considered as the main mediator of infection, and plays a major role in the occurrence of drug resistance. This study was aimed at determining the antimicrobial and antibiofilm activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria. The antimicrobial assay showed that papaya leaf extract exhibits higher activity compared to stevia leaf extract in inhibiting the growth of the biofilm-forming bacteria. The optimum condition of papaya leaf extract to inhibit biofilm-forming bacterial growth occurred at 45% and 75% concentrations of the extract (pH 7). A 100% biofilm degradation by papaya leaf extract occurred at pH 6 and pH 9.

scholarly journals Anti-inflammatory, hypoglycemic and hypolipidemic effects of Stevia rebaudiana bertoni leaf extract in an experimental model of diabetes mellitus and obesity in laboratory animals

2021 ◽  
Vol 11 (5) ◽  
pp. 142-156
Author(s):  
Irina Igorevna Novikova ◽  
◽  
Aleksandr Stepanovich Ogudov ◽  
Irina Gennadyevna Shevkun ◽  
Evgeny Vladimirovich Serenko ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Model ◽  
Leaf Extract ◽  
Stevia Rebaudiana ◽  
Delayed Type Hypersensitivity ◽  
Laboratory Animals ◽  
Educational Settings ◽  
Leaf Extracts ◽  
Stevia Rebaudiana Bertoni ◽  
Anti Inflammatory

Introduction. The paper presents the results of experimental studies focused on assessing the anti-inflammatory, hypoglycemic and hypolipidemic effects of Stevia rebaudiana Bertoni leaf extract. The study was conducted on an experimental model of "zinc" diabetes in white male Wistar rats and under conditions of acute hyperlipidemia caused by the Tween-80 detergent. The aim of the study was to evaluate the feasibility of using Stevia rebaudiana Bertoni leaf extracts in the diet as a natural sweetener for the organizing nutrition of overweight and diabetic people, including educational settings for children, on the model of induced diabetes mellitus and acute hyperlipidemia in laboratory animals. Materials and Methods. In order to solve the research problem, the authors utilized the following techniques: assessing the biochemical parameters of blood serum, determining the level of glucose in the blood, lipid formula and delayed-type hypersensitivity reaction. Theoretical methods involved reviewing and analyzing Russian and international academic articles. Results. The findings of this study suggest that it is possible to use Stevia rebaudiana Bertoni leaf extracts as a natural sugar substitute in the diet of people with overweight and diabetes mellitus, as well as for prophylaxis in healthy people, including meals for children in educational settings. Conclusions. The article concludes that an immunomodulatory effect and the absence of side effects of the Stevia rebaudiana Bertoni leaf extract confirm its usage in the daily diet of the population.

scholarly journals METHOD FOR QUANTIFYING THE AMOUNT OF PHENYLPROPANOIDS IN STEVIA RAW MATERIALS

2020 ◽  
pp. 115-121
Author(s):  
Evgeniy Evgen'yevich Kurdyukov ◽  
Ol'ga Aleksandrovna Vodop'yanova ◽  
Aleksandr Vladimirovich Mitishev ◽  
Yakov Petrovich Moiseev ◽  
Elena Fedorovna Semenova
Keyword(s):  
Raw Materials ◽  
Stevia Rebaudiana ◽  
Raw Material ◽  
Optimal Conditions ◽  
Leaf Extracts ◽  
Stevia Rebaudiana Bertoni ◽  
Single Determination ◽  
Layer Chromatography ◽  
Direct Spectrophotometry

Dried stevia leaves (Stevia rebaudiana Bertoni) were used as objects of research. Four samples of stevia raw materials of different origin were studied: No. 1 (Russia, Penza region), No. 2 (Russia, Republic of Crimea), No. 3 (Paraguay), No. 4 (India). The purpose of this work is to develop a method for quantifying the amount of phenylpropanoids in stevia leaves. Quantitative determination of the amount of phenylpropanoids in stevia leaves by direct spectrophotometry was carried out. Thin-layer chromatography was used to confirm the presence of phenylpropanoids in stevia leaves. The presence of phenylpropanoids in stevia leaf extracts was confirmed by direct spectrophotometry, the analytical maxima of the compounds studied were determined – 290 and 330 nm. The optimal conditions of extraction of phenylpropanoids from raw materials of this plant (extractant – ethyl alcohol 70%; ratio "raw material–extractant" – 1 : 100; extraction time – 45 min; the degree of grinding of raw materials – 1.0 mm). It was determined that the error of a single determination of the content of phenylpropanoids in stevia leaves with a confidence probability of 95% is ±0.44%. It was found that the content of phenylpropanoids in stevia varies in the range of 6.73–10.51%.

Sign in / Sign up

Export Citation Format

Share Document