Adipocyte Size and Leptin Receptor Expression in Human Subcutaneous Adipose Tissue After Roux-en-Y Gastric Bypass

2017 ◽  
Author(s):  
Martha Tamez ◽  
Victoria Ramos-Barragan ◽  
Patricia Mendoza-Lorenzo ◽  
Pablo Arrieta-Joffe ◽  
Sergio López-Martínez ◽  
...  
Keyword(s):  
Gastric Bypass ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Leptin Receptor ◽  
Receptor Expression ◽  
Adipocyte Size ◽  
Subcutaneous Adipose

Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue

2016 ◽  
Vol 12 (2) ◽  
pp. 257-263 ◽  
Author(s):  
Juan José González-Plaza ◽  
Carolina Gutiérrez-Repiso ◽  
Sara García-Serrano ◽  
Francisca Rodriguez-Pacheco ◽  
Lourdes Garrido-Sánchez ◽  
...  
Keyword(s):  
Weight Loss ◽  
Gastric Bypass ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Transcriptomic Profiling ◽  
Subcutaneous Adipose

scholarly journals Growth hormone receptor expression in human gluteal versus abdominal subcutaneous adipose tissue: Association with body shape

Obesity ◽  
2016 ◽  
Vol 24 (5) ◽  
pp. 1090-1096 ◽  
Author(s):  
Kalypso Karastergiou ◽  
Miriam A. Bredella ◽  
Mi-Jeong Lee ◽  
Steven R. Smith ◽  
Susan K. Fried ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Hormone Receptor ◽  
Subcutaneous Adipose Tissue ◽  
Body Shape ◽  
Growth Hormone Receptor ◽  
Receptor Expression ◽  
Hormone Receptor Expression ◽  
Subcutaneous Adipose

scholarly journals Leptin receptors in human skeletal muscle

2007 ◽  
Vol 102 (5) ◽  
pp. 1786-1792 ◽  
Author(s):  
Borja Guerra ◽  
Alfredo Santana ◽  
Teresa Fuentes ◽  
Safira Delgado-Guerra ◽  
Alfredo Cabrera-Socorro ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Molecular Mass ◽  
Protein Level ◽  
Subcutaneous Adipose Tissue ◽  
Human Skeletal Muscle ◽  
Leptin Receptor ◽  
Leptin Receptors ◽  
Subcutaneous Adipose ◽  
Basal Concentration

Human skeletal muscle expresses leptin receptor mRNA; however, it remains unknown whether leptin receptors (OB-R) are also expressed at the protein level. Fourteen healthy men (age = 33.1 ± 2.0 yr, height = 175.9 ± 1.7 cm, body mass = 81.2 ± 3.8 kg, body fat = 22.5 ± 1.9%; means ± SE) participated in this investigation. The expression of OB-R protein was determined in skeletal muscle, subcutaneous adipose tissue, and hypothalamus using a polyclonal rabbit anti-human leptin receptor. Three bands with a molecular mass close to 170, 128, and 98 kDa were identified by Western blot with the anti-OB-R antibody. All three bands were identified in skeletal muscle: the 98-kDa and 170-kDa bands were detected in hypothalamus, and the 98-kDa and 128-kDa bands were detected in thigh subcutaneous adipose tissue. The 128-kDa isoform was not detected in four subjects, whereas in the rest its occurrence was fully explained by the presence of intermuscular adipose tissue, as demonstrated using an anti-perilipin A antibody. No relationship was observed between the basal concentration of leptin in serum and the 170-kDa band density. In conclusion, a long isoform of the leptin receptor with a molecular mass close to 170 kDa is expressed at the protein level in human skeletal muscle. The amount of 170-kDa protein appears to be independent of the basal concentration of leptin in serum.

A note on the effect of adipocyte size on in vitro lipogenesis from acetate and lactate in subcutaneous adipose tissue of large- and small-frame beef steers at 6 months of age

1987 ◽  
Vol 44 (3) ◽  
pp. 454-456 ◽  
Author(s):  
D. C. Rule ◽  
D. C. Beitz ◽  
R. L. Hood
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Chronological Age ◽  
Beef Steers ◽  
Adipocyte Size ◽  
Small Frame ◽  
Beef Producers ◽  
Research Findings ◽  
Subcutaneous Adipose

Beef producers no longer emphasize excessive fat deposition in market cattle. Recent research findings in human health suggest that our diets should contain less fat because of the cardiovascular diseases that seem associated with greater intakes of fat. Large-frame cattle slaughtered at the same chronological age as small-frame cattle have leaner carcasses and a correspondingly greater proportion of carcass protein (Arnold, Hentges and Trenkle, 1985). Determining early in life whether a type of cattle will become obese, therefore, would be of great benefit to cattle producers. Arnold et al. (1985) reported that, at 6 months of age, large-frame steers were significantly leaner than small-frame steers.

scholarly journals Roux-en-Y Gastric Bypass Acutely Decreases Protein Carbonylation and Increases Expression of Mitochondrial Biogenesis Genes in Subcutaneous Adipose Tissue

2015 ◽  
Vol 25 (12) ◽  
pp. 2376-2385 ◽  
Author(s):  
Cyrus Jahansouz ◽  
Federico J. Serrot ◽  
Brigitte I. Frohnert ◽  
Rocio E. Foncea ◽  
Robert B. Dorman ◽  
...  
Keyword(s):  
Gastric Bypass ◽  
Adipose Tissue ◽  
Mitochondrial Biogenesis ◽  
Subcutaneous Adipose Tissue ◽  
Protein Carbonylation ◽  
Subcutaneous Adipose

scholarly journals Bovine tissue mRNA abundance related to acute cold exposure and acute feeding restriction

2005 ◽  
Vol 85 (2) ◽  
pp. 157-164 ◽  
Author(s):  
G. K. Murdoch ◽  
W. T. Dixon ◽  
E. K. Okine ◽  
R. J. Christopherson
Keyword(s):  
Adipose Tissue ◽  
Candidate Genes ◽  
Subcutaneous Adipose Tissue ◽  
Leptin Receptor ◽  
Uncoupling Protein ◽  
Mrna Abundance ◽  
Feed Restriction ◽  
Mesenteric Adipose Tissue ◽  
Npy Receptor ◽  
Subcutaneous Adipose

Thirty-six steers were used to determine the effects of acute cold (-20°C) and acute feed restriction (0.4× maintenance) on mRNA abundance of five candidate genes. To investigate adaptational physiology in cattle we evaluated gene expression patterns for uncoupling protein 2 (UCP-2), uncoupling protein 3 (UCP-3), leptin, leptin receptor and neuropeptide Y receptor (NPY receptor) in specific tissues. We measured relative mRNA abundance using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) on mRNA extracted from duodenum, iver, skeletal muscle, mesenteric adipose tissue, subcutaneous adipose tissue, and peri-renal adipose tissue. We obtained carcass grades for comparison to mRNA abundance profiles. Leptin receptor mRNA abundance was increased by acute feed restriction in the liver (P = 0.02) and trended to increase in the duodenum (P = 0.06) of acute feed-restricted steers. NPY receptor RNA abundance decreased (P = 0.02) in the liver of feed-restricted steers, and acute cold-stress-treated steers (P = 0.03), but increased (P = 0.03) in mesenteric adipose tissue of acute-cold-treated steers. Leptin mRNA abundance was decreased in subcutaneous adipose tissue (P = 0.04) by acute cold, but trended to increase in the peri-renal adipose tissue (P = 0.09). UCP-3 mRNA abundance was not significantly altered by our acute treatments. UCP-2 mRNA abundance increased in subcutaneous adipose tissue of both acute cold (P = 0.004) and acute feed-restricted steers (P = 0.03) and in biceps femoris, after acute cold (P = 0.003) and acute feed restriction (P = 0.002). There was an inverse relationship between the leptin mRNA abundance in subcutaneous adipose and the carcass grade, where the AAA , AA and A grade carcasses had mean values of 501.6 ± 30.0, 535.5 ± 14.0 and 653.4 ± 61.0. Our results show that tissue-specific mRNA abundance of these candidate genes may be altered by acute stressors, and that may relate to tissue-specific adaptational strategies in cattle. Furthermore, leptin mRNA abundance in subcutaneous adipose tissue may inversely reflect the maturity or finishing characteristics of beef cattle such that it is inversely related to the final carcass grade. Key words: Acute cold, acute feed restriction, UCP-2, UCP-3, leptin, leptin receptor, NPY receptor, cattle

An atlas of G-protein coupled receptor expression and function in human subcutaneous adipose tissue

2015 ◽  
Vol 146 ◽  
pp. 61-93 ◽  
Author(s):  
Stefan Amisten ◽  
Matt Neville ◽  
Ross Hawkes ◽  
Shanta J. Persaud ◽  
Fredrik Karpe ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
G Protein ◽  
Subcutaneous Adipose Tissue ◽  
Receptor Expression ◽  
G Protein Coupled Receptor ◽  
And Function ◽  
Subcutaneous Adipose ◽  
G Protein Coupled
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