Improvement of the attenuated mutant strain of Brucella melitensis Rev1 as a potential vaccine candidate

ABSTRACT Clostridium perfringens mutant strain 121A/91 shows neither enzymatic (phospholipase C) nor hemolytic activity. Nevertheless, the cpa gene and the corresponding alpha-toxin variant are detectable. Vaccination with this genetically constructed alpha-toxin variant, rAT121/91, induces antibodies capable of significantly reducing activities induced by wild-type toxin. Thus, rAT121/91 could be a useful vaccine candidate.

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Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate

Veterinary Research ◽

10.1186/s13567-017-0422-9 ◽

2017 ◽

Vol 48 (1) ◽

Cited By ~ 9

Author(s):

Yanqing Bao ◽

Mingxing Tian ◽

Peng Li ◽

Jiameng Liu ◽

Chan Ding ◽

...

Keyword(s):

Mutant Strain ◽

Brucella Abortus ◽

Vaccine Candidate ◽

Potential Vaccine Candidate ◽

Potential Vaccine

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Complete Genome Sequence of NC983, a Live Attenuated Strain ofSalmonella entericaSerovar Typhimurium

Genome Announcements ◽

10.1128/genomea.01074-16 ◽

2016 ◽

Vol 4 (5) ◽

Cited By ~ 2

Author(s):

Bryan Troxell ◽

Ryan C. Fink ◽

Allison N. Dickey ◽

Elizabeth H. Scholl ◽

Hosni M. Hassan

Keyword(s):

Mutant Strain ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Vaccine Candidate ◽

Significant Problem ◽

Potential Vaccine Candidate ◽

Serovar Typhimurium ◽

Potential Vaccine ◽

Foodborne Infections

Foodborne infections caused bySalmonella entericaserovars are a significant problem worldwide. Presented here is the genome sequence of the nontyphoidalS. entericaserovar Typhimurium mutant strain NC983, a potential vaccine candidate.

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Molecular identification and antigenic characterization of Babesia divergens Erythrocyte Binding Protein (BdEBP) as a potential vaccine candidate

Parasitology International ◽

10.1016/j.parint.2017.07.004 ◽

2017 ◽

Vol 66 (6) ◽

pp. 721-726 ◽

Cited By ~ 3

Author(s):

Shimaa Abd El-Salam El-Sayed ◽

Mohamed Abdo Rizk ◽

Mohamad Alaa Terkawi ◽

Naoaki Yokoyama ◽

Ikuo Igarashi

Keyword(s):

Molecular Identification ◽

Vaccine Candidate ◽

Binding Protein ◽

Potential Vaccine Candidate ◽

Antigenic Characterization ◽

Babesia Divergens ◽

Erythrocyte Binding ◽

Potential Vaccine

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Moraxella catarrhalis Bacterium without Endotoxin, a Potential Vaccine Candidate

Infection and Immunity ◽

10.1128/iai.73.11.7569-7577.2005 ◽

2005 ◽

Vol 73 (11) ◽

pp. 7569-7577 ◽

Cited By ~ 68

Author(s):

Daxin Peng ◽

Wenzhou Hong ◽

Biswa P. Choudhury ◽

Russell W. Carlson ◽

Xin-Xing Gu

Keyword(s):

Moraxella Catarrhalis ◽

Lipid A ◽

Vaccine Candidate ◽

Gene Encoding ◽

Potential Vaccine Candidate ◽

Whole Cells ◽

Potential Vaccine ◽

Major Surface ◽

Membrane Components ◽

Human Infections

ABSTRACT Lipooligosaccharide (LOS) is a major surface component of Moraxella catarrhalis and a possible virulence factor in the pathogenesis of human infections caused by this organism. The presence of LOS on the bacterium is an obstacle to the development of vaccines derived from whole cells or outer membrane components of the bacterium. An lpxA gene encoding UDP-N-acetylglucosamine acyltransferase responsible for the first step of lipid A biosynthesis was identified by the construction and characterization of an isogenic M. catarrhalis lpxA mutant in strain O35E. The resulting mutant was viable despite the complete loss of LOS. The mutant strain showed significantly decreased toxicity by the Limulus amebocyte lysate assay, reduced resistance to normal human serum, reduced adherence to human epithelial cells, and enhanced clearance in lungs and nasopharynx in a mouse aerosol challenge model. Importantly, the mutant elicited high levels of antibodies with bactericidal activity and provided protection against a challenge with the wild-type strain. These data suggest that the null LOS mutant is attenuated and may be a potential vaccine candidate against M. catarrhalis.

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