Acaricide activity of the Ximenia americana L. (Olacaceae) stem bark hydroethanolic extract against Rhipicephalus (Boophilus) microplus

Aims: In traditional medicine, several plant species from Rutaceae’s family have been used to treat sickle cell anemia. However, more studies are needed to corroborate the antisickling activity of Zanthoxylum leprieurii species. The objective of this paper was to evaluate the sickling cell inhibition and radical scavenging activities of hydroethanolic and aqueous extracts of Zanthoxylum leprieurii’s bark, a plant species used in the management of sickle cell anemia in eastern Côte d’Ivoire. Methods: Hydroethanolic and aqueous extracts of the stem bark of Zanthoxylum leprieurii were prepared. Qualitative and quantitative phytochemical tests were carried out. In addition, diphenyl 1, 2 picrylhydrazyl (DPPH) was used to determine the antioxidant potential of these two extracts. The antisickling activity of two extracts was determined by the Emmel method. Results and Discussion: Zanthoxylum leprieurii’s barkextracts have demonstrated antioxidant property. The IC50 value of the hydroethanolic extract (0.308±0.06) was lower than that of the decocted (0.434 ±0.06). At 10 mg/mL, the sickling cell inhibition of DZL was 81% ±2.66 while EZL was 89% ±0.44. The presence of alkaloids, sterols, Polyterpenes and phenolic compounds in both extracts could explain the sickling inhibition activity of these extracts. All the extracts revealed an antioxidant and antisickling activities higher than the standard. Conclusion: The hydroethanolic extract (EZL) demonstrated a higher antisickling activity and exhibited a better free radical scavenging activity. The use of Zanthoxylum leprieurii’s bark in the traditional management of sickle cell anemia is justified.

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Hydroethanolic extract of the inner stem bark of Cedrela odorata has low toxicity and reduces hyperglycemia induced by an overload of sucrose and glucose

Journal of Ethnopharmacology ◽

10.1016/j.jep.2014.12.059 ◽

2015 ◽

Vol 162 ◽

pp. 352-361 ◽

Cited By ~ 4

Author(s):

Morenna Alana Giordani ◽

Thiago Carvalho Mamede Collicchio ◽

Sergio Donizeti Ascêncio ◽

Domingos Tabajara de Oliveira Martins ◽

Sikiru Olaitan Balogun ◽

...

Keyword(s):

Stem Bark ◽

Cedrela Odorata ◽

Low Toxicity ◽

Hydroethanolic Extract

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Hydroethanolic Extract of <i>Eribroma oblongum</i> (Malvaceae) Stem Bark Prevents Hypertension, Oxidative Stress and Dyslipidemia in L-NAME Induced Hypertension in Wistar Rats

Journal of Diseases and Medicinal Plants ◽

10.11648/j.jdmp.20160204.11 ◽

2016 ◽

Vol 2 (4) ◽

pp. 43

Author(s):

Tsague Marthe Valentine

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Stem Bark ◽

Induced Hypertension ◽

Hydroethanolic Extract

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Free radical scavenging ability of Ximenia americana L. stem bark and leaf extracts

Journal of Applied Pharmaceutical Science ◽

10.7324/japs.2016.60213 ◽

2016 ◽

pp. 091-096 ◽

Cited By ~ 1

Author(s):

Valdil ocirc,a ◽

Cleyton Sousa ◽

Adonias Carvalho ◽

Ant Sant'Ana ◽

Mariana Chaves

Keyword(s):

Free Radical ◽

Radical Scavenging ◽

Stem Bark ◽

Free Radical Scavenging ◽

Leaf Extracts ◽

Ximenia Americana ◽

Radical Scavenging Ability

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In VitroCytotoxicity andIn VivoAntimammary Tumor Effects of the Hydroethanolic Extract ofAcacia seyal(Mimosaceae) Stem Bark

BioMed Research International ◽

10.1155/2018/2024602 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 5

Author(s):

Stephane Zingue ◽

Amstrong Nang Njuh ◽

Alain Brice Tueche ◽

Jeremie Tamsa ◽

Edwige Nana Tchoupang ◽

...

Keyword(s):

Breast Cancer ◽

Mammary Tumors ◽

Stem Bark ◽

Control Group ◽

Dependent Manner ◽

Antitumor Effects ◽

Hydroethanolic Extract ◽

Acacia Seyal

The present study was designed to evaluate thein vitroandin vivoantitumor effects ofA. seyalhydroethanolic extract on breast cancer. The cytotoxicity ofA. seyalextract was evaluated using resazurin reduction assay in 9 cell lines. Further, the protective effect of the hydroethanolic extract ofA. seyalstem barks was evaluated on 7,12-dimethylbenz(a)anthracene- (DMBA-) induced breast cancer rat model. Incidence, burden, volume, and histological analysis of mammary tumors were measured. TheAcacia seyalextract exhibited CC50of 100 in MCF-7 cells after 24 h.In vivo, no tumors were detected in rats from the control group, while 11 rats out of 12 (91.66%) developed mammary tumors in the DMBA-exposed group receiving only the vehicle.Acacia seyalextract significantly (p<0.01) and in the dose-dependent manner reduced tumor incidence (3 rats out of 12 at the dose of 300 mg/kg), burden [62.1% (150 mg/kg) and 65.8% (300 mg/kg)], and mass. It protected rats against DMBA-induced breast hyperplasia, with an optimal effect at the dose of 300 mg/kg. Taken altogether, these results suggest that the hydroethanolic extract ofAcacia seyalmight contain phytoconstituents endowed with antitumoral properties, which could protect against the breast cancer induced in rats.

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Identification of 3,3′-O-dimethylellagic acid and apigenin as the main antiplasmodial constituents of Endodesmia calophylloides Benth and Hymenostegia afzelii (Oliver.) Harms

BMC Complementary Medicine and Therapies ◽

10.1186/s12906-021-03352-9 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Rodrigue Keumoe ◽

Jean Garba Koffi ◽

Darline Dize ◽

Patrick Valère Tsouh Fokou ◽

Joseph Tchamgoue ◽

...

Keyword(s):

Cell Lines ◽

High Selectivity ◽

Methanolic Extract ◽

Stem Bark ◽

Viability Assay ◽

Crude Extracts ◽

Green Monkey ◽

Bioassay Guided Fractionation ◽

Plant Families ◽

Hydroethanolic Extract

Abstract Background Endodesmia calophylloides and Hymenostegia afzelii belong to the Guttiferae and Caesalpiniaceae plant families with known uses in African ethno-medicine to treat malaria and several other diseases. This study aimed at identifying antiplasmodial natural products from selected crude extracts from H. afzelii and E. calophylloides and to assess their cytotoxicity. Methods The extracts from H. afzelii and E. calophylloides were subjected to bioassay-guided fractionation to identify antiplasmodial compounds. The hydroethanol and methanol stem bark crude extracts, fractions and isolated compounds were assessed for antiplasmodial activity against the chloroquine-sensitive 3D7 and multi-drug resistant Dd2 strains of Plasmodium falciparum using the SYBR green I fluorescence-based microdilution assay. Cytotoxicity of active extracts, fractions and compounds was determined on African green monkey normal kidney Vero and murine macrophage Raw 264.7 cell lines using the Resazurin-based viability assay. Results The hydroethanolic extract of H. afzelii stem bark (HasbHE) and the methanolic extract of E. calophylloides stem bark (EcsbM) exhibited the highest potency against both Pf3D7 (EC50 values of 3.32 ± 0.15 μg/mL and 7.40 ± 0.19 μg/mL, respectively) and PfDd2 (EC50 of 3.08 ± 0.21 μg/mL and 7.48 ± 0.07 μg/mL, respectively) strains. Both extracts showed high selectivity toward Plasmodium parasites (SI > 13). The biological activity-guided fractionation led to the identification of five compounds (Compounds 1–5) from HasbHE and one compound (Compound 6) from EcsbM. Of these, Compound 1 corresponding to apigenin (EC50Pf3D7, of 19.01 ± 0.72 μM and EC50PfDd2 of 16.39 ± 0.52 μM), and Compound 6 corresponding to 3,3′-O-dimethylellagic acid (EC50Pf3D7 of 4.27 ± 0.05 μM and EC50PfDd2 of 1.36 ± 0.47 μM) displayed the highest antiplasmodial activities. Interestingly, both compounds exhibited negligible cytotoxicity against both Vero and Raw 264.7 cell lines with selectivity indices greater than 9. Conclusions This study led to the identification of two potent antiplasmodial natural compounds, 3,3′-O-dimethylellagic acid and apigenin that could serve as starting points for further antimalarial drug discovery.

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