Peripheral Blood Mononuclear Cells (PBMCs) to Dissect the Underlying Mechanisms of Bone Disease in Chronic Kidney Disease and Rare Renal Diseases

The interaction of the fluorescent probe 22-NBD-cholesterol with membranes of human peripheral blood mononuclear cells (PBMC) was tested by time- and spectrally resolved fluorescence imaging to monitor the disturbance of lipid metabolism in chronic kidney disease (CKD) and its treatment with statins. Blood samples from healthy volunteers (HV) and CKD patients, either treated or untreated with statins, were compared. Spectral imaging was done using confocal microscopy at 16 spectral channels in response to 458 nm excitation. Time-resolved imaging was achieved by time-correlated single photon counting (TCSPC) following excitation at 475 nm. The fluorescence of 22-NBD-cholesterol was mostly integrated into plasmatic membrane and/or intracellular membrane but was missing from the nuclear region. The presence of two distinct spectral forms of 22-NBD-cholesterol was uncovered, with significant variations between studied groups. In addition, two fluorescence lifetime components were unmasked, changing in CKD patients treated with statins. The gathered results indicate that 22-NBD-cholesterol may serve as a tool to study changes in the lipid metabolism of patients with CKD to monitor the effect of statin treatment.

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Association of peripheral blood mononuclear cells adenylate cyclase-associated protein 1 mRNA with chronic kidney disease

Atherosclerosis ◽

10.1016/j.atherosclerosis.2017.06.636 ◽

2017 ◽

Vol 263 ◽

pp. e198

Author(s):

Jelena Munjas ◽

Miron Sopic ◽

Vesna Spasojevic-Kalimanovska ◽

Natasa Bogavac-Stanojevic ◽

Sanja Simic-Ogrizovic ◽

...

Keyword(s):

Chronic Kidney Disease ◽

Kidney Disease ◽

Adenylate Cyclase ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Immune dysregulation in peripheral blood mononuclear cells from patients with liver cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2018.36.5_suppl.55 ◽

2018 ◽

Vol 36 (5_suppl) ◽

pp. 55-55

Author(s):

Juhua Zhou

Keyword(s):

Liver Cancer ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Immune Cell ◽

Critical Role ◽

Immune Dysregulation ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Underlying Mechanisms

55 Background: Immune regulation may play an important role in cancer development. The immune dysregulation and underlying mechanisms in patients with liver cancer have not fully understood. Methods: Peripheral blood mononuclear cells (PBMC) were isolated from patients with liver cancer. Deep-sequencing, FACS analysis, ELISA and real-time PCR were used to analyze the immune dysregulation and underlying mechanisms in patients with liver cancer. Results: Our analysis discovered that the percentages of immune cell populations in PBMC from patients with liver cancer were significantly different from those in normal controls. Specifically, the percents of B cells and regulatory T cells were high in PBMC from patients with liver cancer. Expression of 161 genes such as EGF, IRF3, CD177, MAP2K2 and MMP9 was found to be significantly inhibited, but 66 genes including CD19, CD70, FOXP1 and IL-32 were significantly up-regulated in PBMC from patients with liver cancer. Further analysis showed that 190 long non-coding RNAs (lncRNAs) were significantly down-regulated; whereas150 lncRNAs were significantly up-regulated in PBMC from patients with liver cancer. Dysregulated lncRNAs were involved in the control of immune cell signaling, cell division and differentiation. Conclusions: The results suggest that the immune dysregulation may play a critical role in the pathogenesis of liver cancer. It also has a great implication in the development of immune therapeutic methods for patients with liver cancer.

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Age-related features of the pattern of lymphocyte subpopulations and functional activity of peripheral blood mononuclear cells in patients with chronic kidney disease before and after transplantation

Russian Journal of Transplantology and Artificial Organs ◽

10.15825/1995-1191-2020-1-40-49 ◽

2020 ◽

Vol 22 (1) ◽

pp. 40-49

Author(s):

D. V. Artemov ◽

A. B. Zulkarnaev ◽

A. V. Vatazin

Keyword(s):

Chronic Kidney Disease ◽

Kidney Disease ◽

Peripheral Blood Mononuclear Cells ◽

Functional Activity ◽

Mononuclear Cells ◽

Lymphocyte Subpopulations ◽

Peripheral Blood Mononuclear ◽

Age Related ◽

Blood Mononuclear Cells ◽

Before And After

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Metabolic Changes in Peripheral Blood Mononuclear Cells Isolated From Patients With End Stage Renal Disease

Frontiers in Endocrinology ◽

10.3389/fendo.2021.629239 ◽

2021 ◽

Vol 12 ◽

Author(s):

Mehmet M. Altintas ◽

Salvatore DiBartolo ◽

Lana Tadros ◽

Beata Samelko ◽

Haimanot Wasse

Keyword(s):

Kidney Disease ◽

Oxidative Phosphorylation ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Stress Test ◽

Peripheral Blood Mononuclear ◽

Cellular Energy ◽

Blood Mononuclear Cells ◽

End Stage

As numerous complex pathologies stem from cellular energy dysfunction, we aimed to elucidate mitochondrial function and associated stress pathologies in kidney disease in a cohort of hemodialysis patients with end-stage kidney disease (ESKD). The bioenergetics study was conducted using peripheral blood mononuclear cells (PBMCs) of ESKD patients (n = 29) and healthy controls (no ESKD, n = 10). PBMCs were isolated from whole blood and seeded into assay plates to detect changes in oxidative phosphorylation and glycolysis. The bioenergetics analysis (i.e., mitochondrial stress test) was performed using Seahorse XFe24 flux analyzer. We observed significant reduction in mitochondrial respiration in patient PBMCs in terms of fundamental bioenergetics parameters such as basal respiration, ATP turnover, maximal respiration and spare respiratory capacity. These findings were correlated with the expression levels of proteins coordinating cellular energy status and regulating mitochondrial dynamics. Our data demonstrates an association between mitochondrial oxygen consumption of PBMCs and ESKD. AMPK activity, its downstream effector PGC-1α and mitochondrial fission/fusion proteins are partially responsible for the decrease in oxidative phosphorylation of PBMCs isolated from ESKD patients. We propose a link between mitochondrial dysfunction and ESKD and a role for mitochondria as a potential site for therapeutic interventions.

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