Purification of a PHA-Like Chitin-binding Protein from Acacia farnesiana Seeds: A Time-dependent Oligomerization Protein

Background: Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein (Xn-CBP). Objective: The present work aims to clarify the characteristics and function of Xn-CBP from X. nematophila HB310. Methods: In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3). Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide. The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination assay and hyphal extension assay. Results: Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C. diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F. oxysporum, 41.52%, was higher than the other two fungi. Conclusion: The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal activity and antifungal activity. The present study laid a foundation for further exploitation and utilization of X. nematophila.

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Chitin binding protein from the kuruma shrimp Marsupenaeus japonicus facilitates the clearance of Vibrio anguillarum

Developmental & Comparative Immunology ◽

10.1016/j.dci.2020.103981 ◽

2021 ◽

Vol 117 ◽

pp. 103981

Author(s):

Sen Xu ◽

Ming Jing ◽

De-Min Kong ◽

Ya-Ru Wang ◽

Quan Zhou ◽

...

Keyword(s):

Binding Protein ◽

Vibrio Anguillarum ◽

Marsupenaeus Japonicus ◽

Kuruma Shrimp ◽

Chitin Binding Protein

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Phylogenomic Insights into Distribution and Adaptation of Bdellovibrionota in Marine Waters

Microorganisms ◽

10.3390/microorganisms9040757 ◽

2021 ◽

Vol 9 (4) ◽

pp. 757

Author(s):

Qing-Mei Li ◽

Ying-Li Zhou ◽

Zhan-Fei Wei ◽

Yong Wang

Keyword(s):

Comparative Genomics ◽

Deep Sea ◽

Binding Protein ◽

Glycerol Metabolism ◽

Metabolic Reconstruction ◽

Type Ii Secretion ◽

Phylogenetic Groups ◽

Genes Encoding ◽

Epipelagic Zone ◽

Chitin Binding Protein

Bdellovibrionota is composed of obligate predators that can consume some Gram-negative bacteria inhabiting various environments. However, whether genomic traits influence their distribution and marine adaptation remains to be answered. In this study, we performed phylogenomics and comparative genomics studies using 132 Bdellovibrionota genomes along with five metagenome-assembled genomes (MAGs) from deep sea zones. Four phylogenetic groups, Oligoflexia, Bdello-group1, Bdello-group2 and Bacteriovoracia, were revealed by constructing a phylogenetic tree, of which 53.84% of Bdello-group2 and 48.94% of Bacteriovoracia were derived from the ocean. Bacteriovoracia was more prevalent in deep sea zones, whereas Bdello-group2 was largely distributed in the epipelagic zone. Metabolic reconstruction indicated that genes involved in chemotaxis, flagellar (mobility), type II secretion system, ATP-binding cassette (ABC) transporters and penicillin-binding protein were necessary for the predatory lifestyle of Bdellovibrionota. Genes involved in glycerol metabolism, hydrogen peroxide (H2O2) degradation, cell wall recycling and peptide utilization were ubiquitously present in Bdellovibrionota genomes. Comparative genomics between marine and non-marine Bdellovibrionota demonstrated that betaine as an osmoprotectant is probably widely used by marine Bdellovibrionota, and all the marine genomes have a number of genes for adaptation to marine environments. The genes encoding chitinase and chitin-binding protein were identified for the first time in Oligoflexia, which implied that Oligoflexia may prey on a wider spectrum of microbes. This study expands our knowledge on adaption strategies of Bdellovibrionota inhabiting deep seas and the potential usage of Oligoflexia for biological control.

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Chitin binding protein as a possible RNA binding protein in Leishmania parasites

Pathogens and Disease ◽

10.1093/femspd/ftaa007 ◽

2020 ◽

Vol 78 (1) ◽

Author(s):

Sajad Rashidi ◽

Kurosh Kalantar ◽

Celia Fernandez-Rubio ◽

Enayat Anvari ◽

Paul Nguewa ◽

...

Keyword(s):

Rna Binding ◽

Binding Protein ◽

Rna Binding Protein ◽

Novel Proteins ◽

Transcriptional Gene Regulation ◽

Chitin Binding Protein ◽

Effective Drugs ◽

Genus Leishmania ◽

Leishmania Parasites

ABSTRACT Leishmaniasis includes a broad spectrum of pathological outcomes in humans caused by protozoan parasites from the genus Leishmania. In recent years, proteomic techniques have introduced novel proteins with critical functions in Leishmania parasites. Based on our report of a Chitin binding protein (CBP) in our previous immunoproteomic study, this article suggests that CBP might be an RNA binding protein (RBP) in Leishmania parasites. RBPs, as key regulatory factors, have a role in post-transcriptional gene regulation. The presence of RBPs in Leishmania parasites has not been considered so far; however, this study aims to open a new venue regarding RBPs in Leishmania parasites. Confirming CBP as an RBP in Leishmania parasites, exploring other RBPs and their functions might lead to interesting issues in leishmaniasis. In fact, due to the regulatory role of RBPs in different diseases including cancers and their further classification as therapeutic targets, the emerging evaluation of CBP and RBPs from Leishmania parasites may allow the discovery of novel and effective drugs against leishmaniasis.

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Time dependent impact of perinatal hypoxia on growth hormone, insulin-like growth factor 1 and insulin-like growth factor binding protein-3

Metabolic Brain Disease ◽

10.1007/s11011-016-9816-z ◽

2016 ◽

Vol 31 (4) ◽

pp. 827-835 ◽

Cited By ~ 9

Author(s):

Ömer Kartal ◽

Seçil Aydınöz ◽

Ayşe Tuğba Kartal ◽

Taha Kelestemur ◽

Ahmet Burak Caglayan ◽

...

Keyword(s):

Growth Hormone ◽

Growth Factor ◽

Binding Protein ◽

Time Dependent ◽

Insulin Like Growth Factor ◽

Perinatal Hypoxia ◽

Factor Binding

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Gene Expression Profiling of Leiomyoma and Myometrial Smooth Muscle Cells in Response to Transforming Growth Factor-β

Endocrinology ◽

10.1210/en.2004-1377 ◽

2005 ◽

Vol 146 (3) ◽

pp. 1097-1118 ◽

Cited By ~ 72

Author(s):

Xiaoping Luo ◽

Li Ding ◽

Jingxia Xu ◽

Nasser Chegini

Keyword(s):

Gene Expression ◽

Smooth Muscle ◽

Smooth Muscle Cells ◽

Gene Expression Profile ◽

Expression Profile ◽

Binding Protein ◽

Muscle Cells ◽

Time Dependent ◽

Early Gene ◽

A Cell

Altered expression of the TGF-β system is recognized to play a central role in various fibrotic disorders, including leiomyoma. In this study we performed microarray analysis to characterize the gene expression profile of leiomyoma and matched myometrial smooth muscle cells (LSMC and MSMC, respectively) in response to the time-dependent action of TGF-β and, after pretreatment with TGF-β type II receptor (TGF-βRII) antisense oligomer-blocking/reducing TGF-β autocrine/paracrine actions. Unsupervised and supervised assessments of the gene expression values with a false discovery rate selected at P ≤ 0.001 identified 310 genes as differentially expressed and regulated in LSMC and MSMC in a cell- and time-dependent manner by TGF-β. Pretreatment with TGF-βRII antisense resulted in changes in the expression of many of the 310 genes regulated by TGF-β, with 54 genes displaying a response to TGF-β treatment. Comparative analysis of the gene expression profile in TGF-βRII antisense- and GnRH analog-treated cells indicated that these treatments target the expression of 222 genes in a cell-specific manner. Gene ontology assigned these genes functions as cell cycle regulators, transcription factors, signal transducers, tissue turnover, and apoptosis. We validated the expression and TGF-β time-dependent regulation of IL-11, TGF-β-induced factor, TGF-β-inducible early gene response, early growth response 3, CITED2 (cAMP response element binding protein-binding protein/p300-interacting transactivator with ED-rich tail), Nur77, Runx1, Runx2, p27, p57, growth arrest-specific 1, and G protein-coupled receptor kinase 5 in LSMC and MSMC using real-time PCR. Together, the results provide the first comprehensive assessment of the LSMC and MSMC molecular environment targeted by autocrine/paracrine action of TGF-β, highlighting potential involvement of specific genes whose products may influence the outcome of leiomyoma growth and fibrotic characteristics by regulating inflammatory response, cell growth, apoptosis, and tissue remodeling.

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A two component chitin-binding protein from French bean - association of a proline-rich protein with a cysteine-rich polypeptide

FEBS Letters ◽

10.1016/j.febslet.2006.01.079 ◽

2006 ◽

Vol 580 (6) ◽

pp. 1541-1546 ◽

Cited By ~ 30

Author(s):

Laurence V. Bindschedler ◽

Julian P. Whitelegge ◽

David J. Millar ◽

G. Paul Bolwell

Keyword(s):

Binding Protein ◽

French Bean ◽

Two Component ◽

Chitin Binding Protein ◽

Proline Rich Protein

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1H, 13C, 15N resonance assignment of the chitin-binding protein CBP21 from Serratia marcescens

Biomolecular NMR Assignments ◽

10.1007/s12104-010-9281-2 ◽

2010 ◽

Vol 5 (1) ◽

pp. 117-119 ◽

Cited By ~ 5

Author(s):

Finn L. Aachmann ◽

Vincent G. H. Eijsink ◽

Gustav Vaaje-Kolstad

Keyword(s):

Serratia Marcescens ◽

Resonance Assignment ◽

Binding Protein ◽

Chitin Binding Protein

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Leiomyoma and Myometrial Gene Expression Profiles and Their Responses to Gonadotropin-Releasing Hormone Analog Therapy

Endocrinology ◽

10.1210/en.2004-1384 ◽

2005 ◽

Vol 146 (3) ◽

pp. 1074-1096 ◽

Cited By ~ 32

Author(s):

Xiaoping Luo ◽

Li Ding ◽

Jingxia Xu ◽

R. Stan Williams ◽

Nasser Chegini

Keyword(s):

Gene Expression ◽

Signal Transduction ◽

Binding Protein ◽

Expression Profiles ◽

Primary Cultures ◽

Time Dependent ◽

Early Gene ◽

Dependent Manner ◽

Cell Cycle Regulators ◽

Gnrh Analog

Gene microarray was used to characterize the molecular environment of leiomyoma and matched myometrium during growth and in response to GnRH analog (GnRHa) therapy as well as GnRHa direct action on primary cultures of leiomyoma and myometrial smooth muscle cells (LSMC and MSMC). Unsupervised and supervised analysis of gene expression values and statistical analysis in R programming with a false discovery rate of P ≤ 0.02 resulted in identification of 153 and 122 differentially expressed genes in leiomyoma and myometrium in untreated and GnRHa-treated cohorts, respectively. The expression of 170 and 164 genes was affected by GnRHa therapy in these tissues compared with their respective untreated group. GnRHa (0.1 μm), in a time-dependent manner (2, 6, and 12 h), targeted the expression of 281 genes (P ≤ 0.005) in LSMC and MSMC, 48 of which genes were found in common with GnRHa-treated tissues. Functional annotations assigned these genes as key regulators of processes involving transcription, translational, signal transduction, structural activities, and apoptosis. We validated the expression of IL-11, early growth response 3, TGF-β-induced factor, TGF-β-inducible early gene response, CITED2 (cAMP response element binding protein-binding protein/p300-interacting transactivator with ED-rich tail), Nur77, growth arrest-specific 1, p27, p57, and G protein-coupled receptor kinase 5, representing cytokine, common transcription factors, cell cycle regulators, and signal transduction, at tissue levels and in LSMC and MSMC in response to GnRHa time-dependent action using real-time PCR, Western blotting, and immunohistochemistry. In conclusion, using different, complementary approaches, we characterized leiomyoma and myometrium molecular fingerprints and identified several previously unrecognized genes as targets of GnRHa action, implying that local expression and activation of these genes may represent features differentiating leiomyoma and myometrial environments during growth and GnRHa-induced regression.

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