Production of Thermostable Lipase by Thermomyces lanuginosus on Solid-State Fermentation: Selective Hydrolysis of Sardine Oil

2014 ◽  
Vol 174 (5) ◽  
pp. 1859-1872 ◽  
Author(s):  
Nayeli Ávila-Cisneros ◽  
Susana Velasco-Lozano ◽  
Sergio Huerta-Ochoa ◽  
Jesús Córdova-López ◽  
Miquel Gimeno ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Thermomyces Lanuginosus ◽  
Selective Hydrolysis ◽  
Thermostable Lipase ◽  
Sardine Oil ◽  
Hydrolysis Of

Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation

2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Raw Materials ◽  
Hydrolytic Enzymes ◽  
Industrial Wastes ◽  
Added Value ◽  
Orange Peels ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

scholarly journals Optimization Of Culture Conditions For The Production Of Xylanase By Two Thermophilic Fungi Under Solid State Fermentation

2013 ◽  
Vol 39 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Kumkum Azad ◽  
Md. Abdul Halim ◽  
Feroza Hossain
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Culture Conditions ◽  
Maximum Activity ◽  
Thermomyces Lanuginosus ◽  
Thermophilic Fungi ◽  
Thermostable Xylanase ◽  
Optimum Time ◽  
Xylanase Production ◽  
Time Required

Two thermophilic fungi, Thermomyces lanuginosus BPJ-10 and Rhizomucor pusillus BPJ-2 were studied under solid state fermentation (SSF) using wheat bran for the production of thermostable xylanase. The optimum time required for the production of xylanase was found to be 4 days and 7 days for R. pusillus BPJ-2 and T. lanuginosus BPJ-10 respectively. The optimum temperatures for the production of xylanase by R. pusillus BPJ-2 and T. lanuginosus BPJ-10 were 45°C and 50°C respectively. The maximum activity of xylanase (1.685 IU/ml and 0.075 IU/ml) was exhibited by T. lanuginosus BPJ-10 and R. pusillus BPJ-2 at pH 7.0 and pH 4.0 respectively. The optimum moisture content for maximum xylanase production was 90% for both fungi. J. Asiat. Soc. Bangladesh, Sci. 39(1): 43-51, June 2013 DOI: http://dx.doi.org/10.3329/jasbs.v39i1.16032

Optimization of Solid State Fermentation to Improve the Degree of Hydrolysis Soybean Meal Protein

2013 ◽  
Vol 690-693 ◽  
pp. 1239-1242
Author(s):  
Feng Jia ◽  
Bing Qian Han ◽  
Jun Jun Guan ◽  
Guo Hao Yang ◽  
Jin Shui Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Growth Conditions ◽  
Degree Of Hydrolysis ◽  
Optimum Conditions ◽  
Meal Protein ◽  
Hydrolysis Of

In this study, the optimization of soybean meal by solid state fermentation was investigated using temperature of start, the ratio of material to water and inoculums concentration. This work showed that temperature and moisture are the factors that most strongly influence SSF byBacillus subtilisBS-GA15 using soybean meal as substrate. The growth conditions that optimize degree of hydrolysis production are temperature of start at 30 °C, soybean meal and water at a ratio of 1:1.0(w/w), and inoculums concentration at 10%. In optimum conditions degree of hydrolysis of 13.14% was obtained.

Production of Cellulase by Aspergillus niger FC-1 and its Application in Hydrolysis of Corncob Residues

2015 ◽  
Vol 1120-1121 ◽  
pp. 891-896
Author(s):  
Hui Qin Shi ◽  
Zhe Wei Zhao ◽  
Wei Yang ◽  
Di Wu ◽  
Yi Zhao ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Glucose Concentration ◽  
Moisture Ratio ◽  
Ph Stability ◽  
Optimal Values ◽  
Hydrolysis Of

In this study,the cellulase-producing ability of Aspergillusniger FC-1 through solid-state fermentation (SSF) and characteristics of the cellulase were investigated. The maximum activities of total cellulase (FPase) and endoglucanase (CMCase) were 8.2 and 31.5 IU per gram of dried substrate respectively after 96-h incubation period. The activities of both FPase and CMCase produced by Aspergillus niger FC-1 exhibited the optimal values at pH 5.5 and 50°C(as shown in Fig.2). Thermostability and pH stability of the enzymes were respectively appreciable at temperature ranging from 45°C to 55°C, pH ranging from 5.0 to 5.5. In addition, with an optimal 1:10 (w/v) substrate to moisture ratio (a cellulase loading of 8.5 FPU per cellulose), the glucose concentration was as high as 36.6 g glucose l-1 for a 48 h hydrolysis of corncob residues.

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