Influence of Culture Medium Composition and Light Conditions on the Accumulation of Bioactive Compounds in Shoot Cultures of Scutellaria lateriflora L. (American Skullcap) Grown In Vitro

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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Differentiation of mesenchymal stem cells from humans and animals into insulin-producing cells: An overview in vitro induction forms

Current Stem Cell Research & Therapy ◽

10.2174/1574888x16666201229124429 ◽

2020 ◽

Vol 16 ◽

Author(s):

Bruna O. S. Câmara ◽

Bruno M. Bertassoli ◽

Natália M. Ocarino ◽

Rogéria Serakides

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Culture Medium ◽

Adipogenic Differentiation ◽

Medium Composition ◽

Cell Therapies ◽

Insulin Producing Cells ◽

Msc Differentiation

The use of stem cells in cell therapies has shown promising results in the treatment of several diseases, including diabetes mellitus, in both humans and animals. Mesenchymal stem cells (MSCs) can be isolated from various locations, including bone marrow, adipose tissues, synovia, muscles, dental pulp, umbilical cords, and the placenta. In vitro, by manipulating the composition of the culture medium or transfection, MSCs can differentiate into several cell lineages, including insulin-producing cells (IPCs). Unlike osteogenic, chondrogenic, and adipogenic differentiation, for which the culture medium and time are similar between studies, studies involving the induction of MSC differentiation in IPCs differ greatly. This divergence is usually evident in relation to the differentiation technique used, the composition of the culture medium, the cultivation time, which can vary from a few hours to several months, and the number of steps to complete differentiation. However, although there is no “gold standard” differentiation medium composition, most prominent studies mention the use of nicotinamide, exedin-4, ß-mercaptoethanol, fibroblast growth factor b (FGFb), and glucose in the culture medium to promote the differentiation of MSCs into IPCs. Therefore, the purpose of this review is to investigate the stages of MSC differentiation into IPCs both in vivo and in vitro, as well as address differentiation techniques and molecular actions and mechanisms by which some substances, such as nicotinamide, exedin-4, ßmercaptoethanol, FGFb, and glucose, participate in the differentiation process.

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Efficiency of using different types induction culture medium for hexaploid triticale anther cultivation

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v25.1174 ◽

2019 ◽

Vol 25 ◽

pp. 260-265

Author(s):

E. V. Lagunovskaya ◽

O. I. Zaitseva ◽

V. A. Lemesh

Keyword(s):

Culture Medium ◽

Medium Composition ◽

Induction Medium ◽

Hexaploid Triticale ◽

Culture Methods ◽

Study Results ◽

Short Period ◽

Androgenesis In Vitro ◽

The Republic

Aim. Triticale is one of the main grain crops of the Republic of Belarus. Further progress in the selection of this culture involves the accelerated creation of highly productive early ripening varieties resistant to abiotic and biotic factors. The method of induced androgenesis in vitro makes it possible to obtain stable homozygous lines in a short period of time and to eliminate the lengthy process of inbreeding used in classical breeding to fix the desired traits. Methods. The tissue and cell culture methods for plants was used in the study. Results. The influence of the induction medium composition on the efficiency of in vitro induced androgenesis in varieties and lines of hexaploid triticale is assessed. The influence of three types of induction culture medium, the type of phytohormones and the presence or absence of cefotaxime in the medium are analyzed. Results. It has been shown that using the C-17 culture medium supplemented with 2.0 mg/l 2,4-D and 0.5 mg/l kinetin without adding cefotaxime is most effective for the anther triticale cultivation. Keywords: triticale, anther culture, induction nutrient medium, embryoids, calli, regenerant plants, cefotaxime.

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In vitro development of one-cell embryos from outbred mice: Influence of culture medium composition

In Vitro Cellular & Developmental Biology ◽

10.1007/bf02624106 ◽

1990 ◽

Vol 26 (2) ◽

pp. 151-156 ◽

Cited By ~ 10

Author(s):

Akiko Spindle

Keyword(s):

Culture Medium ◽

Medium Composition ◽

In Vitro Development ◽

Outbred Mice ◽

Vitro Development

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Micropropagation of Juvenile Eucalyptus regnans (Mountain Ash)

Australian Journal of Botany ◽

10.1071/bt9910179 ◽

1991 ◽

Vol 39 (2) ◽

pp. 179 ◽

Cited By ~ 11

Author(s):

C Blomstedt ◽

J Cameron ◽

P Whiteman ◽

SF Chandler

Keyword(s):

Woody Plant ◽

Basal Medium ◽

High Light Intensity ◽

Shoot Cultures ◽

Light Conditions ◽

Indolebutyric Acid ◽

Eucalyptus Regnans ◽

Mountain Ash ◽

Napthaleneacetic Acid

Node-derived shoot cultures of Eucalyptus regnans were established from in vitro grown seedlings on Murashige and Skoog basal medium supplemented with 0.5 mg L-1 (2 μm) zeatin and 0.05 mg L-1 (0.3 μm) napthaleneacetic acid. A double sterilisation method was essential to obtain clean material from seed. Microcuttings from established cultures were used to develop an efficient method for in vitro rooting. Rooting was best after a 7 day pulse on 20 mg L-1 (98 μm) indolebutyric acid. Hoagland's or Woody Plant Medium supported better rooting than MS basal medium and rooting was significantly enhanced by subculture to activated charcoal after the auxin pulse. Carbohydrate (sucrose or glucose) was essential for rooting while high light intensity was inhibitory. Optimal light conditions were a 12 h day (17 W m-2). In all, 90% of plantlets established in the nursery survived the winter.

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Micropropagation of Cyclopia genistoides, an Endemic South African Plant of Economic Importance

Zeitschrift für Naturforschung C ◽

10.1515/znc-2012-1-209 ◽

2012 ◽

Vol 67 (1-2) ◽

pp. 65-76 ◽

Cited By ~ 1

Author(s):

Adam Kokotkiewicz ◽

Maria Luczkiewicz ◽

Anna Hering ◽

Renata Ochocka ◽

Krzysztof Gorynski ◽

...

Keyword(s):

South African ◽

Sucrose Concentration ◽

Shoot Multiplication ◽

Potassium Nitrate ◽

Economic Importance ◽

Medium Composition ◽

Root Induction ◽

Multiplication Rate ◽

Shoot Cultures

An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 μM 6-(γ,γ-dimethylallylamino)purine (2iP) and 1.0 μM thidiazuron (TDZ). Maximum shoot multiplication rate [(8.2 ± 1.3) microshoots/explant)] was observed on this medium composition. Prior to rooting, the multiplied shoots were elongated for 60 days (two 30-days passages) on SH medium with one-half sucrose concentration, supplemented with 4.92 μM indole-3-butyric acid (IBA). The rooting of explants was only possible in the case of the elongated shoots. The highest root induction rate (54.8%) was achieved on solid SH medium with one-half sucrose and one-half potassium nitrate and ammonium nitrate concentration, respectively, supplemented with 28.54 μM indole-3-acetic acid (IAA) and 260.25 μM citric acid. The plantlets were acclimatized for 30 days in the glasshouse, with the use of peat/gravel/perlite substrate (1:1:1). The highest acclimatization rate (80%) was obtained for explants rooted with the use of IAA-supplemented medium. The phytochemical profile of the regenerated plants was similar to that of the reference intact plant material. HPLC analyses showed that C. genistoides plantlets obtained by the micropropagation procedure kept the ability to produce xanthones (mangiferin and isomangiferin) and the fl avanone hesperidin, characteristic of wild-growing shrubs.

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Culture medium for mint micropropagation in vitro

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-88 ◽

2020 ◽

Author(s):

N.A. Yegorova ◽

◽

M.S. Zagorskaya ◽

O.V. Yakimova ◽

◽

...

Keyword(s):

In Vitro Propagation ◽

Culture Medium ◽

Medium Composition ◽

Multiplication Rate ◽

Ms Medium ◽

Second Stage ◽

Clonal Micropropagation ◽

Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

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Long-term in Vitro Regeneration of Hamelia patens

HortScience ◽

10.21273/hortsci.30.4.873g ◽

1995 ◽

Vol 30 (4) ◽

pp. 873G-874

Author(s):

D. Sankhla ◽

T.D. Davis ◽

N. Sankhla ◽

A. Upadhyaya

Keyword(s):

Culture Medium ◽

In Vitro Regeneration ◽

Shoot Formation ◽

Shoot Tips ◽

Shoot Cultures ◽

Ms Medium ◽

Prolific Shoot ◽

Ornamental Shrub

This report describes an efficient in vitro regeneration protocol for H. patens (firebush), a heat-tolerant ornamental shrub native to tropical and subtropical America. Shoot cultures were initially established using shoot tips placed on MS-revised medium containing 2.3 μM 2,4-D, 2.3 μM kinetin, and 0.25% polyvinylpyrrolidone. Other types of explants (nodal and internodal segments, leaf pieces, floral buds) did not regenerate shoots when placed on this medium. Two-month-old plantlets derived from the shoot tips were subcultured on MS medium supplemented with 0.5 μM thidiazuron (TDZ), and within 3 to 4 weeks, some callus was produced at the root–shoot junction. When this callus, with a small portion of the root and shoots, was placed on MS medium with 0.05 μM TDZ and 0.01 μM ABA, prolific shoot formation occurred within 3 to 4 weeks followed by root formation. By regular subculturing every 5 to 6 weeks, hundreds of plantlets have been obtained over the past 3 years with no apparent decline in regeneration potential. Addition of activated charcoal (0.5%) to the culture medium has greatly improved growth of the plantlets.

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Enhanced Seed Germination and Seedling Growth of Tillandsia Eizii In Vitro

HortScience ◽

10.21273/hortsci.38.1.101 ◽

2003 ◽

Vol 38 (1) ◽

pp. 101-104 ◽

Cited By ~ 19

Author(s):

Kimberly A. Pickens ◽

James M. Affolter ◽

Hazel Y. Wetzstein ◽

Jan H.D. Wolf

Keyword(s):

Seed Germination ◽

In Vitro Culture ◽

Seedling Growth ◽

Culture Medium ◽

Habitat Destruction ◽

Light Conditions ◽

Physiological Constraints ◽

Large Numbers ◽

In The Wild

Tillandsia eizii is an epiphytic bromeliad that due to over-collection, habitat destruction, and physiological constraints has declined to near threatened status. This species exhibits high mortality in the wild, and seed are characterized by low percentages of germination. As a means to conserve this species, in vitro culture protocols were developed to enhance seed germination and seedling growth. A sterilization protocol using 70% ethanol for 2 minutes followed by 2.6% NaOCl for 40 minutes disinfested seed and promoted seedling growth. Sucrose incorporated into the culture medium had no effect on germination or growth, while NAA inhibited growth, but not germination. Cultures maintained under a 16-hour photoperiod at 22 °C exhibited greater growth than those grown at 30 °C. Seed that germinated in the dark remained etiolated and failed to develop even after transfer to light conditions. Plants grown in vitro were successfully acclimatized and transferred to the greenhouse. Over 86% survival and rapid growth were obtained with either an all-pine-bark medium, or a mixture of 2 redwood bark: 2 fir bark: 2 potting mix: 1 perlite. This demonstrated that in vitro culture of seed may be used to rapidly produce large numbers of T. eizii, and thus can be used for the conservation and reintroduction of this species.

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