scholarly journals Expression, Purification, and Characterization of the Recombinant, Two-Component, Response Regulator ArlR from Fusobacterium nucleatum

2022 ◽  
Author(s):  
Ruochen Fan ◽  
Zhuting Li ◽  
Xian Shi ◽  
Lulu Wang ◽  
Xuqiang Zhang ◽  
...  
Keyword(s):  
Response Regulator ◽  
Fusobacterium Nucleatum ◽  
Purification And Characterization ◽  
Two Component

scholarly journals Molecular Characterization of Two-Component Systems of Helicobacter pylori

2000 ◽  
Vol 182 (8) ◽  
pp. 2068-2076 ◽  
Author(s):  
Dagmar Beier ◽  
Rainer Frank
Keyword(s):  
Helicobacter Pylori ◽  
Histidine Kinase ◽  
Response Regulator ◽  
Response Regulators ◽  
Reading Frame ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems

ABSTRACT Two-component systems are frequently involved in the adaptation of bacteria to changing environmental conditions at the level of transcriptional regulation. Here we report the characterization of members of the two-component systems of the gastric pathogenHelicobacter pylori deduced from the genome sequence of strain 26695. We demonstrate that the response regulators HP166, HP1043, and HP1021 have essential functions, as disruption of the corresponding genes is lethal for the bacteria, irrespective of the fact that HP1043 and HP1021 have nonconserved substitutions in crucial amino acids of their receiver domains. An analysis of the in vitro phosphorylation properties of the two-component proteins demonstrates that HP244-HP703 and HP165-HP166 are cognate histidine kinase-response regulator pairs. Furthermore, we provide evidence that the variability of the histidine kinase HP165 caused by a poly(C) tract of variable length close to the 3′ end of open reading frame 165/164 does not interfere with the kinase activity of the transmitter domain of HP165.

Characterization of Interactions between a Two-Component Response Regulator, Spo0F, and Its Phosphatase, RapB†

Biochemistry ◽  
1998 ◽  
Vol 37 (47) ◽  
pp. 16538-16545 ◽  
Author(s):  
Yih-Ling Tzeng ◽  
Victoria A. Feher ◽  
John Cavanagh ◽  
Marta Perego ◽  
James A. Hoch
Keyword(s):  
Response Regulator ◽  
Two Component

An orphan two-component response regulator Slr1588 involves salt tolerance by directly regulating synthesis of compatible solutes in photosynthetic Synechocystis sp. PCC 6803

2014 ◽  
Vol 10 (7) ◽  
pp. 1765-1774 ◽  
Author(s):  
Lei Chen ◽  
Lina Wu ◽  
Ye Zhu ◽  
Zhongdi Song ◽  
Jiangxin Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Response Regulator ◽  
Compatible Solutes ◽  
Two Component ◽  
Synechocystis Sp ◽  
Pcc 6803

We report here the characterization of a novel orphan response regulator Slr1588 directly involved in the synthesis and transport of compatible solutes against salt stress.

scholarly journals Candida albicans SRR1 , a Putative Two-Component Response Regulator Gene, Is Required for Stress Adaptation, Morphogenesis, and Virulence

2011 ◽  
Vol 10 (10) ◽  
pp. 1370-1374 ◽  
Author(s):  
Chirayu Desai ◽  
John Mavrianos ◽  
Neeraj Chauhan
Keyword(s):  
Candida Albicans ◽  
Response Regulator ◽  
Stress Adaptation ◽  
Regulator Gene ◽  
Content Type ◽  
Virulence Attenuation ◽  
Two Component ◽  
Resistance To Stress ◽  
Identification And Characterization

ABSTRACT We report here the identification and characterization of a previously uncharacterized, two-component response regulator gene (orf19.5843) from Candida albicans . Because of its apparent functions in stress adaptation, we have named this gene SRR1 ( s tress r esponse r egulator 1). Disruption of SRR1 causes defects in hyphal development, reduced resistance to stress, and severe virulence attenuation in the mouse model of disseminated candidiasis.

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