Sweet pepper varieties for Western Siberia

Представлены результаты селекционной работы по культуре перца сладкого. Показано влияние основных факторов среды на рост растений перца в условиях резко континентального климата Западной Сибири. Освещены некоторые направления сортовой селекции для необогреваемых теплиц и открытого грунта. Главное направление селекции – создание скороспелых сортов с высокой потенциальной продуктивностью, использование которых позволит получать не только гарантированный урожай, но и успешно вести семеноводство. Экспериментальные данные получены в условиях Западной Сибири (г. Барнаул, Алтайский край) при выращивании перца в открытом грунте и необогреваемых пленочных теплицах (весенне-летний культурооборот) на ЗСООС – филиале ФГБНУ ФНЦО. Селекционную работу вели с 2000 года и по настоящее время. Оценку селекционного материала проводили в 2015–2021 годах. Материалом для исследований служили коллекционные, селекционные образцы перца различного генетического и эколого-географического происхождения, а также созданные сорта. Представленные сорта перца пластичны, обладают комплексом хозяйственно ценных признаков: высокой урожайностью, хорошим качеством продукции, устойчивостью к стрессовым факторам и основным болезням. Наиболее востребована группа сортов с конусовидными и пирамидальными плодами (Сибирский князь, Подарок лета, Кавалер, Солнечная улыбка, Золотая пирамида), урожайность которых 4–6 кг/м2 в теплице, в открытом грунте – 26–42 т/га, содержание витамина С – 114–248 мг%, пригодные для салатов, цельноплодного консервирования, замораживания и фарширования. Сорта Солнечная улыбка и Золотая пирамида с плодами оранжевой и желтой окраски в биологической спелости незаменимы при украшении блюд. Крупноплодные сорта с плодами призмовидно-кубовидной формы различной окраски в технической спелости: Викинг (темно-зеленая) и Вальс (светло-зеленая) востребованы в течение всего сезона, особенно в период заготовок впрок. Урожайность этой группы сортов в теплице – 4–7 кг/м2, в открытом грунте – 26–39 т/га, содержание витамина С – 120–191 мг%. Сорта перспективны для выращивания в Сибири, способны удовлетворить высокие требования овощеводов и потребителей. The results of breeding work on the culture of sweet pepper are presented. The influence of the main environmental factors on the growth of pepper plants in the sharply continental climate of Western Siberia is shown. Some directions of varietal selection for unheated greenhouses and open ground are highlighted. The main direction of breeding is the creation of precocious varieties with high potential productivity, the use of which will allow not only to obtain a guaranteed harvest, but also to successfully conduct seed production. Experimental data were obtained in the conditions of Western Siberia (Barnaul, Altai Krai) when growing pepper in the open ground and unheated film greenhouses (spring-summer cultural turnover) at the WSVES – branch of the FSBSI FSVC. Breeding work was carried out from 2000 to the present. The selection material was evaluated in 2015–2021. The materials for research were collectible, breeding samples of pepper of various genetic and ecological-geographical origin, as well as created varieties. The presented varieties of pepper are plastic, have a complex of economically valuable characteristics: high yield, good product quality, resistance to stress factors and major diseases. The most popular group of varieties with cone-shaped and pyramidal fruits (Sibirskii knyaz», Podarok leta, Kavaler, Solnechnaya ulybka, Zolotaya piramida), the yield of which is 4–6 kg/m2 in a greenhouse, in the open ground – 26–42 t/ha, vitamin C content – 114–248 mg%, suitable for salads, whole-fruit canning, freezing and stuffing. Varieties Solnechnaya ulybka and Zolotaya piramida with orange and yellow fruits in biological ripeness are indispensable for decorating dishes. Large-fruited varieties with prismatic-cuboid fruits of various colors in technical ripeness: Viking (dark green) and Val's (light green) are in demand throughout the season, especially during the harvesting period for the future. The yield of this group of varieties is 4–7 kg/m2 in a greenhouse, in the open ground – 26–39 t/ha, vitamin C content 120–191 mg%. The varieties are promising for cultivation in Siberia, able to meet the high demands of vegetable growers and consumers.

Overexpression of NMNAT3 improves mitochondrial function and enhances anti-oxidative stress of bone marrow mesenchymal stem cells via the NAD+-Sirt3 pathway

Oxidative stress damage is a common problem in bone marrow mesenchymal stem cell (BMSC) transplantation. Under stress conditions, the mitochondrial function of BMSCs is disrupted, which accelerates senescence and apoptosis of BMSCs, ultimately leading to poor efficacy. Therefore, improving mitochondrial function and enhancing the anti-oxidative stress capacity of BMSCs may be an effective way of improving the survival rate and curative effect of BMSCs. In this study, we have confirmed that overexpression of nicotinamide mononucleotide adenylyl transferase 3 (NMNAT3) improves mitochondrial function and resistance to stress-induced apoptosis in BMSCs. We further revealed the mechanism of NMNAT3-mediated resistance to stress-induced apoptosis in BMSCs. We increased the level of nicotinamide adenine dinucleotide (NAD+) by overexpressing NMNAT3 in BMSCs and found that it could significantly increase the activity of silent mating type information regulation 2 homolog 3 (Sirt3) and significantly decrease the acetylation levels of Sirt3-dependent deacetylation-related proteins isocitrate dehydrogenase 2 (Idh2) and Forkhead-box protein O3a (FOXO3a). These findings show that NMNAT3 may increase the activity of Sirt3 by increasing NAD+ levels. Our results confirm that the NMNAT3-NAD+-Sirt3 axis is a potential mechanism for improving mitochondrial function and enhancing anti-oxidative stress of BMSCs. In this study, we take advantage of the role of NMNAT3 in inhibiting stress-induced apoptosis of BMSCs and provide new methods and ideas for breaking through the bottleneck of transplantation efficacy of BMSCs in the clinic.

Innovative Study on Microstructural Change in Duplex Stainless Steel in Corrosive Media

Duplex Stainless Steel was developed long back in 1930 and gradually finds its wide application because of its high strength, good weldability, good toughness and resistance to stress corrosion cracking. This alloy finds its application in pressure vessels, bridges, process plants and also in typically down to minus 50 degree centigrade applications. However, because of its high alloy content thermal conductivities of duplex stainless steel are low. Casting this alloy is difficult and can be industrially used after proper heat treatment. In this present study corrosion rates were measured for CD4MCu in terms of weight loss/unit area/hour and microstructures were observed in different corrosive medium with time as variable.

FORMATION OF A HEALTHY LIFESTYLE AS A CURRENT DIRECTION OF SOCIAL AND PEDAGOGICAL WORK WITH ADOLESCENTS

The Healthy lifestyle`s formation is an actual problem of pedagogy, psychology and social work. The issues of saving, restoring health, and resistance to stress have become extremely relevant. The results of research of adolescents’ awareness of a healthy lifestyle are represented in the article. Teens are well informed about the basic concepts of a healthy lifestyle as detailed analysis showed. The process of forming responsibility for own life and health, conscious decision-making and overcoming cultural stereotypes about a healthy way of life requires special attention. The issues of saving, restoring health, and resistance to stress have become extremely relevant. Therefore, the analysis of the awareness of teens in matters of a healthy lifestyle has become necessary for the formation of a further algorithm for saving and restoring health. The analysis of research determined alcohol is an acceptable attribute that does not seriously interfere with a healthy lifestyle. This cognitive malformation is correlated with the promotion of alcohol and cigarettes on TV and web, also these harmful elements are acceptable in national traditions. The correlation between family`s healthy lifestyle and resistance to stress is noticed. The families which respect a healthy lifestyle are more resistible to stresses during a pandemic time. It is manifested in more adequate statements and constructive predictions about the use of trends in health lifestyle`s technologies. The correlation between family`s healthy lifestyle and resistance to stress is noticed. The families which respect a healthy lifestyle are more resistible to stresses during a pandemic time. It is manifested in more adequate statements and constructive predictions about the use of trends in health lifestyle`s technologies The most part of teens is trying to follow the healthy lifestyle`s rules and avoid harmful substances. Teenagers are interested in maintaining and restoring physical and psychological health during a pandemic. The gender aspect, processes of conscious behavior acceptance of consequences and motivation for the formation of responsible behavior require additional researches.

Ageing Resistance of Bi-Layer Coating Systems for Dry Lubrication of Premium OCTG Connections

The latest dope-free configuration combines an electrodeposited zinc-nickel (ZnNi) plating, which provides anti-galling and most of anticorrosion properties, and an organic topcoat which provides lubrication through its low friction coefficient. This dry lubrication constitutes an alternative to storage and running dope meanwhile it improves running performances, reduces operational costs on the yard and rig and avoids dope discharge to the environment. Since the technology is "rig-ready", it must withstand the different risks of degradation occurring along its whole lifecycle. The present study aims at assessing the robustness towards ageing along storage on yards, transportation to the rig and or service life in well conditions. The performances of the different layers were checked stepwise, first assessing the ZnNi plating alone, and then considering the additional protection brought by thermoset topcoat. Regarding atmospheric corrosion, the characterization path involved both accelerated laboratory tests (such as the VDA 233-102 cyclic corrosion test) and outdoor exposures, under plastic protectors and after their removal, in different climates: temperate, desertic and tropical. The specimens were inspected regarding at: (i) efficiency of cathodic protection provided by the metallic coating; (ii) paint blistering, (iii) propagation of corrosion from a scribe down to substrate. Regarding rig operations, some examples of rig-return were reported and the compatibility with completion fluids, encountered in case of misrun and subsequent pull-out of the column, was checked though immersion in alkaline brines. In respect to the service in simulated well conditions, the resistance to Stress Corrosion Cracking (SCC) in brines were carried out to complete the former autoclave tests to assess resistance of carbon and stainless steel to well conditions. Both the ZnNi plating and the bi-layer system revealed lifetimes in storage conditions ranging from 3 to more than 5 years before any sign of significant degradation such as red rust, paint blistering or disbonding. According to cyclic corrosion tests results, higher lifetimes could be even expected thanks to the additional anticorrosion protection of the topcoat. Regarding exposure to completion fluids, the bilayer coating was shown to withstand 3000h exposure with no more than scarce rust indications. These results testify of the technology robustness from storage on yards to rig operations. In the multiple service conditions in wells, it was shown that the corrosion and cracking resistance of the substrate was not deteriorated by the plating presence, but instead improved in the multiple assessed well service conditions. The present communication updates the results of atmospheric corrosion compared to the former one [1] and it details new results after rig-return and regarding the risks of cracking.

SOGAZ-Med experience in conducting training for employees of a medical organization in the city of Ivanovo

In the context of the ongoing COVID-19 epidemic, the issues of maintaining medical workers’ psychological resistance to stress and preventing their professional burnout are becoming urgent. SOGAZ-Med regularly holds events dedicated to patient orientation and conflict resolution.

Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm—2.5 mm, the diameter of the bacteria was 0.5 μm—1.0 μm × 2.0 μm—6.3 μm, and the spore diameter was 1 μm—1.2 μm × 1 μm—1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.

Physiological and biochemical mechanisms of plants resistance to oxidative stress under peptide elicitor AtPep1

The effect of the peptide elicitor AtPep1 on the resistance of soybean and pea plants to oxidative stress was studied. The concentration of the peptide 10– 9 mol/L has the maximum elicitor effect on these plants. It was shown that treatment of the aerial part of seedlings with this peptide leads to an increase in the activity of peroxidase and superoxide dismutase and a decrease in the level of lipid peroxidation products in plants under oxidative stress. Revealed effects cause an increase in the plants resistance to stress.