Effects of Zinc Supplementation During In Vitro Maturation on Meiotic Maturation of Oocytes and Developmental Capacity in Yak

The chinchilla is a hystricomorph rodent with a natural habitat in the Andes mountains of Chile (see review by Boussarie 2002 Proc. 27th WSAVA Congr.). For most of the chinchilla subspecies, the decline in the natural population can be attributed to human destruction of the native ecosystems and hunting for fur. Chinchillas are listed as a protected endangered species, at immediate risk of extinction. In Europe, chinchillas are reared for pets and fur production. The female has a seasonal polyestrous reproductive activity with a breeding season from November to May. The estrous cycle length is variable (28–41 days), with an estrous duration of 2 days. After a gestation of about 112 days, a litter of 1 to 6 young is born (see reviews by Morrow 1986 in Current Therapy in Theriogenology 2, W.B. Saunders; and Collot 1998 in Proc. I EVSSAR Congr.). Reproductive biotechniques in this species could play an important role in managing both captive and natural populations as well as in sustaining and improving genetic and global biodiversity. The specific aim of this preliminary work was to standardize an efficient in vitro maturation (IVM) procedure for Chinchilla laniger oocytes so that it will be possible, in the future, to perform IVF and embryo cryopreservation and transfer. Oocytes from 4 cyclic breeding females were recovered by slicing ovaries, obtained by ovariohysterectomy, and were matured in vitro according to the procedure described for bovine oocytes by Dell'Aquila et al. (2002 Mol. Reprod. Dev. 63, 210–222). Two trials of 2 estrous subjects each were performed, on the basis of behavioral signs of estrous and vaginal cytology (Harris-Schorr staining), in the early and late breeding seasons. During estrus, the vaginal smear consisted of superficial cells, further neutrophils, and small and large intermediates, whereas parabasal cells were not found. At the end of the culture time, oocytes were stained with Hoechst 33258 and evaluated for the stage of meiotic maturation. Three out of 4 oocytes recovered in November (75%) reached full meiotic maturation, showing the second metaphase plate and the first polar body (PB) extruded. The fourth oocyte, showing the first PB together with multiple pronuclear structures, was classified as activated. On the contrary, none out of 12 oocytes recovered in May reached full maturation. Of them, 7 (58%) remained at the germinal vesicle stage, 2 (17%) reached metaphase I, and 3 (25%) showed abnormally dispersed chromatin configuration. To our knowledge, this is the first study reporting that chinchilla oocytes can be matured in vitro by bovine IVM procedures. Even though the number of oocytes was poor, we can hypothesize that oocytes from C. laniger are best collected in the breeding season when subjected to an IVM technique.

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Biphasic in vitro maturation (CAPA-IVM) specifically improves the developmental capacity of oocytes from small antral follicles

Journal of Assisted Reproduction and Genetics ◽

10.1007/s10815-019-01551-5 ◽

2019 ◽

Vol 36 (10) ◽

pp. 2135-2144 ◽

Cited By ~ 10

Author(s):

Flor Sanchez ◽

Anh H. Le ◽

Vu N. A. Ho ◽

Sergio Romero ◽

Heidi Van Ranst ◽

...

Keyword(s):

In Vitro Maturation ◽

Antral Follicles ◽

Developmental Capacity

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Zinc supplementation of vitrification medium improves in vitro maturation and fertilization of oocytes derived from vitrified-warmed mouse ovaries

Cryobiology ◽

10.1016/j.cryobiol.2016.12.007 ◽

2017 ◽

Vol 74 ◽

pp. 31-35 ◽

Cited By ~ 4

Author(s):

Shirin Geravandi ◽

Mehri Azadbakht ◽

Mahsa Pourmoradi ◽

Fatemeh Nowrouzi

Keyword(s):

In Vitro Maturation ◽

Zinc Supplementation

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Bovine oocytes treated prior to in vitro maturation with a combination of butyrolactone I and roscovitine at low doses maintain a normal developmental capacity

Molecular Reproduction and Development ◽

10.1002/mrd.1123 ◽

2001 ◽

Vol 60 (4) ◽

pp. 579-585 ◽

Cited By ~ 53

Author(s):

Nunzia Ponderato ◽

Irina Lagutina ◽

Gabriella Crotti ◽

Paola Turini ◽

Cesare Galli ◽

...

Keyword(s):

In Vitro Maturation ◽

Low Doses ◽

Developmental Capacity ◽

Bovine Oocytes

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Embryo development, oocyte morphology, and kinetics of meiotic maturation in bovine oocytes exposed to 6-dimethylaminopurine prior to in vitro maturation

Molecular Reproduction and Development ◽

10.1002/(sici)1098-2795(199807)50:3<334::aid-mrd10>3.0.co;2-4 ◽

1998 ◽

Vol 50 (3) ◽

pp. 334-344 ◽

Cited By ~ 47

Author(s):

Birthe Avery ◽

Anders Hay-Schmidt ◽

Poul Hyttel ◽

Torben Greve

Keyword(s):

Embryo Development ◽

In Vitro Maturation ◽

Meiotic Maturation ◽

Oocyte Morphology ◽

Bovine Oocytes ◽

Kinetics Of

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321 OOCYTE DIAMETER INFLUENCES THE MEIOTIC RESUMPTION AND PROGRESSION INDUCED BY OKADAIC ACID IN DOG

Reproduction Fertility and Development ◽

10.1071/rdv18n2ab321 ◽

2006 ◽

Vol 18 (2) ◽

pp. 268

Author(s):

F. Ariu ◽

L. Bogliolo ◽

I. Rosati ◽

M. T. Zedda ◽

S. Pau ◽

...

Keyword(s):

Okadaic Acid ◽

In Vitro Maturation ◽

Mammalian Species ◽

Cumulus Cells ◽

Meiotic Maturation ◽

Control Group ◽

Oocyte Diameter ◽

Treatment Groups ◽

Different Diameters

The acquisition of meiotic competence, in the bitch as in many other mammalian species, is related to the oocyte diameter. This study was designed to determine the effect of okadaic acid (OA), a potent inhibitor of seronine/threonine 1 and 2A phosphatases, on meiotic resumption and progression in canine oocytes with different diameters. In two experiments, healthy cumulus-oocytes complexes were collected from ovaries of bitches at various stages of the estrous cycle and divided, by diameters, into three treatment groups for in vitro maturation: <110 �m, 110-120 �m, and >120 �m. In Experiment 1, oocytes were pre-incubated for 1 h in TCM-199 + 20% estrous canine serum (SCE) + cysteamine + OA (0.5 �M). Then, oocytes were cultured for 48 h in the same medium without OA at 38.5�C, 5% CO2 in air. As a control group, oocytes were matured in vitro under the same conditions but without pre-incubation with OA. In Experiment 2, to determine if the effect of OA is mediated by cumulus cells, >120 �m oocytes were denuded from cumulus cells, incubated with or without OA, and cultured in vitro as previously described. At 48 h, all oocytes were stained and fixed with glycerol-Hoechst 33342 to assess the stage of meiotic maturation. In Experiment 1, OA induced a significantly higher incidence of meiotic resumption in oocytes <110 �m (16/108, 14.8%; P < 0.05) and 110-120 �m (70/130, 53.8%; P < 0.01) as compared to that of oocytes in the <110 �m and 110-120 �m control groups (2/58, 3.4%; 24/82, 29.3%). The percentage of oocytes in the 110-120 �m OA group that underwent in vitro maturation to metaphase II (MII) was significantly higher than in the 110-120 �m control group (18/130, 13.8% vs. 4/82, 4.9%, respectively; P < 0.05). In contrast, smaller oocytes (<110 �m) did not develop to MII with or whitout OA. Meiotic resumption rate of >120 �m OA group (64/78, 82.0%) was similar to the >120 �m control group (56/72, 77.8%), but a significantly higher proportion of the oocytes pre-incubated with OA progressed to MII than did the control oocytes (40/78, 51.3% vs. 12/72, 16.7%, respectively; P < 0.01). Low rates of meiotic resumption were observed in denuded >120-�m oocytes with (7/63, 11.1%) or without OA (7/55, 12.7%) and none of them progressed to MII. In conclusion, the results of the present study indicate that treatment of fully grown (>120 �m) oocytes with okadaic acid at the onset of in vitro maturation can result in a higher frequency of meiotic maturation than previously reported. Also, we determined that the beneficial effect of okadaic acid was mediated by cumulus cells.

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224. Disruption of bi-directional oocyte-cumulus paracrine signalling during oocyte in vitro maturation reduces subsequent mouse fetal survival

Reproduction Fertility and Development ◽

10.1071/srb08abs224 ◽

2008 ◽

Vol 20 (9) ◽

pp. 24

Author(s):

C. X. Yeo ◽

R. B. Gilchrist ◽

M. Lane

Keyword(s):

In Vitro Maturation ◽

Cumulus Cell ◽

Meiotic Maturation ◽

Fetal Outcomes ◽

F1 Hybrid ◽

Substantial Impact ◽

Paracrine Signalling ◽

Fetal Survival ◽

First Polar Body

During folliculogenesis, oocyte to cumulus cell (CC) bi-directional communication is essential for normal development of the oocyte. We recently showed that addition of recombinant oocyte paracrine factor growth differentiation factor 9 (GDF9) during mouse oocyte in vitro maturation (IVM) increased fetal viability. GD.F. 9 signals through SMAD 2/3. Hence the effects of disrupting SMAD2/3 signalling and its interaction with FSH/EGF during IVM on oocyte development and subsequent fetal outcomes were investigated. Cumulus-oocyte complexes (COCs) from antral follicles (n = 400–500) of eCG treated pre-pubertal (C57BL/6xCBA F1 hybrid) mice were cultured for 18 h in Waymouth's medium+5% serum, with or without 50 mIU/mL FSH and 10ng/mL EGF, SMAD2/3 inhibitor SB-431542 (4µM), or its 0.04% DMSO control. Meiotic maturation was assessed by first polar body (PB1) extrusion immediately after culture. COCs were fertilised and cultured to the blastocyst stage in G1.2/G2.2 media at 37°C in 6%CO2:5%O2:89%N2. Blastocysts were either transferred to pseudo-pregnant Swiss females or differentially stained. Pregnancy outcome was assessed on Day 18 of pregnancy. Inhibition of SMAD 2/3 signalling did not alter meiotic maturation. No differences were observed in the percentage of blastocysts or hatching blastocysts from cleaved embryos with SMAD2/3 inhibition or the absence of FSH/EGF. However, IVM with SB-431542 or without FSH/EGF significantly decreased (P < 0.001) blastocyst inner cell mass percentages (26% v. 35% control;18% v. 28% control respectively). Fetal survival (fetuses per embryo transferred) of oocytes matured with SB-431542 was significantly decreased (30% v. 50% controls; P < 0.05) although implantation rates and subsequent fetal weights were unaffected. These findings demonstrate the importance of oocyte-CC communication throughout IVM. Inhibition of oocyte signalling through SMAD2/3 resulted in reduced blastocyst quality and fetal survival; outcomes similar to that of oocytes matured without FSH/EGF. Oocyte–cumulus cell bi-directional communication is thus an important feature of oocyte viability and has a substantial impact on subsequent fetal outcomes.

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Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems

Reproduction Fertility and Development ◽

10.1071/rd14022 ◽

2015 ◽

Vol 27 (7) ◽

pp. 1082 ◽

Cited By ~ 5

Author(s):

Maricy Apparicio ◽

Giuliano Q. Mostachio ◽

Tathiana F. Motheo ◽

Aracelle E. Alves ◽

Luciana Padilha ◽

...

Keyword(s):

In Vitro Maturation ◽

Germinal Vesicle ◽

Meiotic Maturation ◽

Cortical Granule ◽

Cortical Granules ◽

Germinal Vesicle Breakdown ◽

System A ◽

Cytoplasmic Maturation ◽

Positive Effect

The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72 h in base medium (BM) with 10 IU mL–1 human chorionic gonadotrophin (hCG), 1 μg mL–1 progesterone (P4) and 1 μg mL–1 oestradiol (E2); in bi-phasic System B oocytes were matured for 48 h in BM with hCG and for 24 h in BM with P4; in bi-phasic System C oocytes were matured for 48 h in BM with hCG, P4 and E2, and for 24 h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P < 0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P < 0.001). The stage of the oestrous cycle did not influence maturation rates.

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