Effect of Low-Intensity Microwave Radiation on Monoamine Neurotransmitters and Their Key Regulating Enzymes in Rat Brain

Molecular indicators reflecting the states of stress-limiting systems of mononuclear leucocytes in the blood, as well as the effects of low-intensive microwave radiation in patients with coronary artery disease were studied. The work it was evaluated the content in mononuclear leucocytes whole blood of components PI3P/AKT/mTOR/p70S6K1 of signaling pathway, heat shock proteins (HSP27, HSP70, HSP90), the concentra-tion of antioxidants and peroxides depending on the level of phosphorylation of the terminal protein kinase MAPK/SAPK of signaling pathway – p38. The results of the study. It was revealed the dependence of a level of studied factors from the degree of phosphorylation p38 in patients with coronary heart disease. It was defined the 38 p sensitivity to the effects of low-intensity microwave radiation, it is manifested by increased level of phosphorylation in the irradiated cul-tures. This study revealed the sensitivity to low-intensity microwave irradiation of content in the mononuclear cells phosphorylated forms of the protein kinases AMPK, AKT1, p70S6K1, as well as the antioxidant status and protein of p53-dependent initial content in the cell phosphorylated form p38. It was shown a possibility of microwave radiation to reduce the content in the cells of the protein kinase and p70 ACT more pronounced at high levels of phosphorylation p38.

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The condition of the production of cytokines, soluble co-stimulatory molecules, intracellular signaling pathways and nitric oxide in patients with coronary heart disease on the background of low-intensity microwave therapy

Journal of New Medical Technologies eJournal ◽

10.12737/18560 ◽

2016 ◽

Vol 10 (1) ◽

pp. 0-0 ◽

Cited By ~ 1

Author(s):

Аржников ◽

V. Arzhnikov ◽

Логаткина ◽

A. Logatkina ◽

Бондарь ◽

...

Keyword(s):

Coronary Heart Disease ◽

Acute Coronary Syndrome ◽

Heart Disease ◽

Angina Pectoris ◽

Microwave Radiation ◽

Interstitial Fluid ◽

Radiation Frequency ◽

Coronary Syndrome ◽

Low Intensity ◽

Caspase 1

The concentration of cytokines of the family IL-10 (IL-10, IL-22, IL-24), interferon-gamma, soluble forms of co-stimulatory molecules CD28, CD80, CD152, as well as NO was investigated in patients with coronary heart disease in interstitial fluid. In addition, the level of caspase-1, inducible and endothelial forms of NO synthasis (eNOS, iNOS), protein kinase AKT1 and AMPK and the total antioxidant activity from the cell culture supernantat were evaluatedin the agranulocytic patients. In the current study (in vitro) the impact on production of these mediators of low-intensity microwave radiation frequency of 1000 MHz, generated by an apparatus of low-intensity physiotherapy "Aquaton" was evaluated. Material of this study was whole venous blood of patients with angina pectoris and acute coronary syndrome. The analysis of the results revealed a reduced level of NO, IL-24, as well as increased production of IL-10, IL-22 and the concentration of soluble forms of CD28, CD80, CD152 in patients with coronary artery disease in the interstitial fluid in comparison with practically healthy persons. At a single irradiation of cell cultures whole blood by low-intensity microwave radiation frequency of 1000 MHz in patients with angina pectoris and acute coronary syndrome there were an increasing of NO production by 20,6 and 25,5‰, IL-24 by 18,6 and 33,6‰, and a reduction in intracellular caspase-1 by 32,5 and 25,8‰, as well as increasing of the levels of IL-10 by 3,5 and 3,1‰, IL-22 by 28,7 and 26,5‰ and reduction of the levels of CD28 by 3,6 and 3,9‰, CD80 6,8 and 5,7‰, CD152 by 9,4 and 11,3‰, respectively. In addition, in terms of irradiation there is an increase in interstitial fluid levels of antioxidants by 37,1 and 49,2‰.

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Cytokine production by whole blood cells of the reconvalescents of community-acquired pneumonia under the influence of low-intensity microwave irradiation

Journal of New Medical Technologies eJournal ◽

10.12737/5025 ◽

2014 ◽

Vol 8 (1) ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Никифоров ◽

V. Nikiforov ◽

Терехов ◽

I. Terekhov ◽

Хадарцев ◽

...

Keyword(s):

Microwave Radiation ◽

Whole Blood ◽

Blood Cells ◽

Cytokine Production ◽

Cell Activity ◽

Community Acquired Pneumonia ◽

Tnf Alpha ◽

Toll Like Receptor ◽

Low Intensity ◽

Whole Blood Cells

The study discusses the effect on the functional state of the whole blood cells of low-intensity microwave radiation. On the model of intercellular interactions in the conditions of spontaneous cell activity and by stimulating cells complex mitogen, the influence of microwave radiation 1000 MHz on production by whole blood cells interleukins: IL-1β, 2, 6, 8, 10, 12, 13, 17A, TNF-alpha, IFN-g, RAIL-1, and soluble forms of toll-like receptor 1, 2, 4, 6 types was studied. The research results show that a single 45 minutes of exposure of microwave radiation of 0.05 PPM microwatt/cm2 has the ability to enhance mitogen-stimulated production of RAIL-1 by 38,7% (p=0.03), to reduce the induced production of IL-1β by 26,3% (p=0,037), IL-8 by 56,2% (p=0.022) and to increase oppressed segment complex mitogen production of IL-10 by 27,8% (p=0,041). The microwave radiation increased spontaneous and mitogen-stimulated expression of TLR, especially TLR1 during the initial low level.

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