EST Sequencing of Meloidogyne javanica Infected Pineapple Root Tissues Reveals Changes in Gene Expression during Root-Knot Nematode Induced Gall Formation

2014 ◽  
Vol 7 (2) ◽  
pp. 43-52 ◽  
Author(s):  
Richard L. Moyle ◽  
Jose R. Botella
Keyword(s):  
Gene Expression ◽  
Meloidogyne Javanica ◽  
Gall Formation ◽  
Root Knot Nematode ◽  
Est Sequencing ◽  
Root Tissues

scholarly journals Trigonella foenum-graecum (fenugreek)-mediated suppression of Meloidogyne javanica in mungbean

2013 ◽  
Vol 55 (2) ◽  
pp. 121-132 ◽  
Author(s):  
Tayyaba Zia ◽  
Imran A. Siddiqui ◽  
Syed S. Shaukat ◽  
Syed Nazaru-Hasnai
Keyword(s):  
Meloidogyne Javanica ◽  
Macrophomina Phaseolina ◽  
Fungal Communities ◽  
Root Knot Nematode ◽  
Egg Hatch ◽  
Soil Suppressiveness ◽  
Juvenile Mortality ◽  
Trigonella Foenum Graecum ◽  
Root Tissues ◽  
Population Numbers

Soil amendments with powdered seeds of <i>Trigonella foenum - graecum</i> (fenugreek) caused soil suppressiveness against <i>Meloidogyne javanica</i>. Decomposed seeds of fenugreek caused marked reduction in nematode population densities and subsequent root-knot development as compared to the aqueous extract of the seeds indicating that some indirect factors are involved in the suppression of root-knot nematode. Both decomposed seeds and aqueous extracts enhanced plant height and fresh weights of shoot whereas root growth remained uninfluenced. Changes in fungal communities associated with nematode control were studied by comparing population numbers of fungi in the soil and in internal root tissues (endorhiza) in non-amended and fenugreekamended soils. <i>Acremonium</i> sp., <i>Chaetomium globosum, Fusarium solani, Macrophomina phaseolina</i> and <i>Rhizoctonia solani</i> were found to colonize inner root tissues of mungbean. <i>Acremonium</i> sp., <i>C. globosum</i> and <i>F.solani</i> were isolated in a relatively higher frequency from roots growing in the amended soils while <i>M. phaseolina</i> and <i>R. solani</i> colonized greatly in roots growing in non-amended soil. Of the fungi isolated from soils, <i>Penicillium brefaldianum</i> caused maximum juvenile mortality of <i>M.javanica</i> whereas <i>F.solani</i> caused greatest inhibition of egg hatch.

scholarly journals Control of the root-rot and root-knot disease complex by Pseudomonas aeruginosa: impact of bacterial rhizosphere colonization

2013 ◽  
Vol 54 (1) ◽  
pp. 55-64
Author(s):  
I. A. Siddiqui ◽  
S. Ehteshamul-Haque ◽  
S. S.. Shaukat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fusarium Solani ◽  
Root Rot ◽  
Meloidogyne Javanica ◽  
Macrophomina Phaseolina ◽  
Root Infection ◽  
Gall Formation ◽  
Root Knot Nematode ◽  
Rhizosphere Colonization ◽  
Growth Promoting

The potential of 3 <i>Pseudomonas aeriuginosa</i> strains as biocontrol agents of rootinfecting fungi <i>Macrophomina phaseolina, Fusarium solani</i> and <i>Rhizoctonia solani</i> and the root-knot nematode <i>Meloidogyne javanica</i> was tested on chili and uridbean under greenhouse conditions. All the three strains significantly reduced nematode populations in soil, invasion, multiplication and gall formation due to <i>M.javanica</i>. Root infection by fungi was also effectively suppressed following <i>P.aeruginosa</i> application. Bacterial antagonists exhibited better biocontrol and growth promoting activity in 15-day-old plants than did those harvested at 30 or 45 days. Population of the bacterium in the rhizosphere declined rapidly after 15 days of nematode inoculation. Strain Pa-5 showed maximum nodulation in 15-day-old samplings while strain Pa-7 showed highest number of nodules in 30 and 45-day-old uridbean plants.

scholarly journals Mineral composition of beetroot treated with potential elicitors and inoculated with Meloidogyne javanica

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Paula Juliana Grotto Débia ◽  
Beatriz Cervejeira Bolanho ◽  
Claudia Regina Dias-Arieira
Keyword(s):  
Population Density ◽  
Mineral Composition ◽  
Integrated Management ◽  
Meloidogyne Javanica ◽  
Nematode Population ◽  
Root Knot Nematode ◽  
Cu Content ◽  
Manganese Zinc ◽  
Nematode Population Density ◽  
Elicitor Treatments

Abstract Background The root-knot nematode Meloidogyne javanica can infect beetroots, causing extensive damage to this food crop. As chemical and genetic control tactics have shown limited efficacy, new strategies are needed to improve the integrated management of this parasite. This study assessed the influence of potential defence elicitors and M. javanica infection on the mineral composition of beetroot. Plants were treated with acibenzolar-S-methyl (ASM), citrus biomass, or a mannanoligosaccharide-based product (MOS) and inoculated with 1000 eggs and second-stage juveniles of M. javanica. At 60 days after inoculation, beetroot plants were harvested and evaluated for nematode population density, vegetative growth, and mineral content. Results All potential elicitors reduced nematode population density in beetroots (p ≤ 0.10) and improved the vegetative parameters of inoculated plants (p ≤ 0.05), except shoot fresh weight. Some minerals were found to be negatively affected by treatments, particularly calcium, whose levels were consistently lower in treated plants. On the other hand, M. javanica inoculation increased magnesium, iron, manganese, zinc, and copper contents in beetroots. However, the latter mineral (Cu content) of inoculated plants was positively influenced by MOS and ASM. Conclusion Potential elicitor treatments did not improve the mineral composition of beetroot, but were effective in reducing nematode population density. Plants inoculated with M. javanica had higher mineral levels. However, gall formation decreases the commercial value of the crop and might render it unsuitable for commercialisation. M. javanica-infected beetroots may be used for nutrient extraction or sold to food processing industries.

The effect of organic compost of Caryocar brasiliense waste against Meloidogyne javanica in two vegetable crops

Nematology ◽  
2021 ◽  
pp. 1-8
Author(s):  
Fabíola de J. Silva ◽  
Regina C.F. Ribeiro ◽  
Adelica A. Xavier ◽  
Vanessa A. Gomes ◽  
Paulo V.M. Pacheco ◽  
...  
Keyword(s):  
Integrated Control ◽  
Meloidogyne Javanica ◽  
Control Measures ◽  
Vegetable Crops ◽  
Root Knot Nematode ◽  
Second Stage ◽  
Tomato Roots ◽  
Crop Development ◽  
Caryocar Brasiliense ◽  
Organic Compost

Summary Root-knot nematodes (Meloidogyne spp.) are responsible for various significant crop losses, which require taking integrated control measures. The present study aimed to identify a possible sustainable approach to the management of Meloidogyne javanica in vegetable crops using an organic compound based on pequi (Caryocar brasiliense) fruit residues. A pot experiment was conducted using cultivars of tomato and lettuce susceptible to M. javanica, with three amendments including inorganic fertiliser, cattle manure and five doses of organic compost with pequi residues. All treatments were inoculated with second-stage juveniles of M. javanica to simulate the root-knot nematode disease in field conditions. Increasing doses of organic compost with pequi residues from 5 kg m−3 to 30 kg m−3 promoted a significant decrease in the nematode population in both cultures evaluated. Organic compost (30 kg m−3) reduced the numbers of galls and eggs of M. javanica by 41.6 and 46.5% in tomato roots, and by 80.3 and 59.2% in lettuce roots, respectively, compared with non-treated control. Organic compost also increased crop development considerably. In general, there was a 43.0% increase in plant development compared to non-treated control. Hence, organic compost of pequi residues could be an alternative to toxic chemical nematicides and recommended as eco-friendly management of M. javanica in vegetable crops.

scholarly journals First record of infection of Papaya trees with root-knot nematode (Meloidogyne javanica) in Australia

2008 ◽  
Vol 3 (1) ◽  
pp. 87 ◽  
Author(s):  
Modika R. Perera ◽  
Ruben D. Flores-Vargas ◽  
Michael G. K. Jones
Keyword(s):  
Meloidogyne Javanica ◽  
First Record ◽  
Root Knot Nematode

scholarly journals Genome expression dynamics reveals parasitism regulatory landscape of the root-knot nematode Meloidogyne incognita and a promoter motif associated with effector genes

2021 ◽  
Author(s):  
Martine Da Rocha ◽  
Caroline Bournaud ◽  
Julie Dazeniere ◽  
Peter Thorpe ◽  
Clement Pellegrin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Meloidogyne Incognita ◽  
Life Stage ◽  
Regulation Of Gene Expression ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Control Of Gene Expression ◽  
Precise Control ◽  
Effector Genes ◽  
Spatio Temporal

Root-knot nematodes are the major contributor to the crop losses caused by nematodes. Root-knot nematodes secrete effectors into the plant, derived from two sets of pharyngeal gland cells, to manipulate host physiology and immunity. Successful completion of the life cycle, involving successive molts from egg to adult, covers morphologically and functionally distinct stages and will require precise control of gene expression, including effectors. The details of how root-knot nematodes regulate transcription remain sparse. Here, we report a life stage-specific transcriptome of Meloidogyne incognita. Combined with an available annotated genome, we explore the spatio-temporal regulation of gene expression. We reveal gene expression clusters and predicted functions that accompany the major developmental transitions. Focusing on effectors, we identify a putative cis-regulatory motif associated with expression in the dorsal glands: providing an insight into effector regulation. We combine the presence of this motif with several other criteria to predict a novel set of putative dorsal gland effectors. Finally, we show this motif, and thereby its utility, is broadly conserved across the Meloidogyne genus and termed it Mel-DOG. Taken together, we provide the first genome-wide analysis of spatio-temporal gene expression in a root-knot nematode, and identify a new set of candidate effector genes that will guide future functional analyses.

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