A Cost-Effective Spectrophotometric Method Based on Enzymatic Analysis of Jackfruit Latex Peroxidase for the Determination of Carbaryl and Its Metabolite 1-Napthol Residues in Organic and Chemical-Free Vegetables

2019 ◽  
Vol 13 (2) ◽  
pp. 433-444 ◽  
Author(s):  
Pirom Didpinrum ◽  
Kraingkrai Ponhong ◽  
Watsaka Siriangkhawut ◽  
Sam-ang Supharoek ◽  
Kate Grudpan
Keyword(s):  
Spectrophotometric Method ◽  
Cost Effective ◽  
Enzymatic Analysis

scholarly journals Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

2013 ◽  
Vol 6 (1) ◽  
pp. 133-141 ◽  
Author(s):  
S. Binte Amir ◽  
M. A. Hossain ◽  
M. A. Mazid
Keyword(s):  
Spectrophotometric Method ◽  
Cost Effective ◽  
Cefuroxime Axetil ◽  
Pharmaceutical Formulation ◽  
Uv Spectrum ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Label Claim ◽  
Development And Validation

The present study was undertaken to develop and validate a simple, sensitive, accurate, precise and reproducible UV spectrophotometric method for cefuroxime axetil using methanol as solvent. In this method the simple UV spectrum of cefuroxime axetil in methanol was obtained which exhibits absorption maxima (?max) at 278 nm. The quantitative determination of the drug was carried out at 278 nm and Beer’s law was obeyed in the range of (0.80-3.60) µg/ml. The proposed method was applied to pharmaceutical formulation and percent amount of drug estimated (95.6% and 96%) was found in good agreement with the label claim. The developed method was successfully validated with respect to linearity, specificity, accuracy and precision. The method was shown linear in the mentioned concentrations having line equation y = 0.05x + 0.048 with correlation coefficient of 0.995. The recovery values for cefuroxime axetil ranged from 99.85-100.05. The relative standard deviation of six replicates of assay was less than 2%. The percent relative standard deviations of inter-day precision ranged between 1.45-1.92% and intra-day precision of cefuroxime axetil was 0.96-1.51%. Hence, proposed method was precise, accurate and cost effective.  Keywords: UV-Vis spectrophotometer; Method validation; Cefuroxime axetil; Recovery studies.  © 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.   doi: http://dx.doi.org/10.3329/jsr.v6i1.14879 J. Sci. Res. 6 (1), 133-141 (2013)  

Development of a highly sensitive and selective spectrophotometric method for the determination of carvedilol in pharmaceutical and urine samples

RSC Advances ◽  
2014 ◽  
Vol 4 (77) ◽  
pp. 40816-40823
Author(s):  
Masoud Shariati-Rad ◽  
Mohsen Irandoust ◽  
Sara Sheikhi
Keyword(s):  
Spectrophotometric Method ◽  
Cost Effective ◽  
Urine Samples ◽  
Highly Sensitive

A simple, sensitive, selective, accurate and cost-effective spectrophotometric method for the determination of CAR in pharmaceutical and urine samples was developed.

scholarly journals Determination of Choline in Milk and Infant Formulas by Enzymatic Analysis: Collaborative Study

2000 ◽  
Vol 83 (1) ◽  
pp. 131-138 ◽  
Author(s):  
David C Woollard ◽  
Harvey E Indyk
Keyword(s):  
Acid Hydrolysis ◽  
Infant Formula ◽  
Spectrophotometric Method ◽  
Collaborative Study ◽  
Analytical Techniques ◽  
Choline Oxidase ◽  
Infant Formulas ◽  
Enzymatic Analysis ◽  
Data Screening

Abstract A collaborative study was conducted on a coupled enzymatic–spectrophotometric method for the determination of choline in infant formula and milk powders. Twenty-nine laboratories participated in the analysis of 8 blind duplicates over the range of 45–175 mg/100 g sample. After the combined acid hydrolysis–phospholipase release of choline, incubation with choline oxidase in the presence of peroxidase and phenol generates a quinoneimine chromophore with 4-aminoantipyrine. Following raw data screening, overall mean RSDR was estimated at 5.14% (range, 4.26–6.34%) with a HORRAT value of 0.91 (range, 0.76–1.00) and an overall mean RSDr:RSDR value of 0.53 (range, 0.42–0.74). The method was also compared with alternative independent analytical techniques for several of the collaborative study samples.

scholarly journals UV Spectrophotometric Method Development and Validation of Fimasartan Drug and Its Tablet Formulation

1970 ◽  
Vol 7 (5) ◽  
pp. 25-29
Author(s):  
Kaushik S Agrawal ◽  
Lokesh R Gandhi ◽  
Nitin S. Bhajipale S Bhajipale3
Keyword(s):  
Standard Deviation ◽  
Quantitative Determination ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Method Development ◽  
Cost Effective ◽  
Tablet Formulation ◽  
Relative Standard ◽  
Bulk Drug

A novel, safe and sensitive method of Spectrophotometric estimation in UV - region has been developed for the assay of Fimasartan in its tablet formulation. The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV spectrophotometric method for the estimation of Fimasartan bulk and pharmaceutical formulation. The method have been developed and validated for the assay of Fimasartan using Methanol as diluent. Absorption maximum (λmax) of the drug was found to be 240nm. The quantitative determination of the drug was carried out at 240nm. The method was shown linear in the mentioned concentrations having correlation coefficient R2 of 0.999. The recovery values for Fimasartan ranged from 98.74% to 99.23%.The Percent Relative Standard Deviation of interday and intraday was 0.85% and 0.75% respectively. All the parameters of the analysis were chosen according to the International Conference on Harmonisation guideline and validated statistically using Relative Standard Deviation and Percent Relative Standard Deviation. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.   KEY WORDS: 

scholarly journals Comparative Study on Quantification of Total Catechins Using UV-Vis Spectrophotometric Method and High Performance Liquid Chromatography Techniques

2021 ◽  
Vol 37 (1) ◽  
pp. 136-142
Author(s):  
Sitharanjan Kalidass ◽  
Karuppannau Daiyarvijaya ◽  
Rajagopal Raj Kumar
Keyword(s):  
Quantitative Analysis ◽  
Spectrophotometric Method ◽  
Mobile Phase ◽  
High Performance ◽  
Cost Effective ◽  
Hplc Method ◽  
Good Resolution ◽  
Huge Number ◽  
Automated Hplc

Bioavailability of catechinsin wider range of plants was established earlier and it’s utility as medicine against cardiovascular disease, cancer, etc. were also demonstrated. Recent techniques in relation to quantitative analysis of total catechins seem to be laborious and time consuming process to handle huge number of samples. Established spectrophotometry and HPLC methods developed earlier for quantitative determination of total catechins in tea extracts were compared in the present study.UV-Vis spectrophotometric method was adopted to monitor the absorbance at 500 nm of the reaction mixture (catechins and vanillin-H2SO4reagents). Hewlett Packard automated HPLC was used and equipped with Phenomenex Luna 5  phenyl-hexyl column fitted with a Phenomenex guard column. Binary elution was carried out using Mobile phase A (acetic acid and acetonitrile) and Mobile phase B (acetonitrile). Method adopted showed a good resolution of catechin fractions and was found to be accurate for the quantification of total catechins (sum of individual catechins). Results of the both the methods are comparable and variation amongst the two methods ranged between -3.59 and 2.79% among the clones and varied with seasons.As expected UPASI released tea clones exhibited variations in their bioavailability. Lean season edge over the cropping period sampling in terms of total catechins. Results obtained from both the methods are comparable. Two methods can be used for the routine quantitative analysis of total catechins; however, spectrophotometric method found to be simple, rapid and cost effective than that of HPLC method unless individual catechins composition is warranted.

scholarly journals Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies

2014 ◽  
Vol 50 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Edith Cristina Laignier Cazedey ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Quality Control ◽  
Hydrochloric Acid ◽  
Spectrophotometric Method ◽  
Cost Effective ◽  
Pharmaceutical Formulation ◽  
Dissolution Studies ◽  
Linearity Range ◽  
Development And Validation ◽  
Routine Quality Control

New, simple and cost effective UV-spectrophotometric method was developed for the estimation of orbifloxacin in pharmaceutical formulation. Orbifloxacin was estimated at 290 nm in 0.5 M hydrochloric acid. Linearity range was found to be 1.0-6.0 μg mL-1. The method was tested and validated for various parameters according to main guidelines. The proposed method was successfully applied for the determination of orbifloxacin in tablets. The results demonstrated that the procedure is accurate, precise and reproducible, while being simple, economical and less time consuming. It can be suitably applied for the estimation of orbifloxacin in routine quality control and dissolution studies.

scholarly journals Method developed for the determination of apixaban by using U.V. spectrophotometric

2019 ◽  
Vol 1 (3) ◽  
pp. 83-87
Author(s):  
B. Mahendra ◽  
K. Harika Sundari ◽  
T. Vimalakkannan
Keyword(s):  
Spectrophotometric Method ◽  
Cost Effective ◽  
Dosage Forms ◽  
Routine Analysis ◽  
Uv Spectrophotometry ◽  
Pharmaceutical Dosage Forms

The present work is aim to Develop UV spectrophotometry method for the estimation of Apixaban in its dosage forms. Analysed the marketed formulations for their reliability and accuracy and Performed the recovery studies for the developed UV spectrophotometric method. The developed method was validate for its accuracy precision reproducibility. On the basis of results the UV spectrophotometric method developed for the determination of Apixaban is found to be precise, accurate and cost effective. Hence this method can be used for routine analysis of Apixaban in bulk and pharmaceutical dosage forms.

scholarly journals Simple UV Spectrophotometric Method for the Determination of Fluvastatin Sodium in Bulk and Pharmaceutical Formulations

2009 ◽  
Vol 6 (4) ◽  
pp. 1233-1239 ◽  
Author(s):  
J. Saminathan ◽  
A. S. Sankar ◽  
K. Anandakumar ◽  
T. Vetrichelvan
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Regression Line ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Good Precision ◽  
Limit Of Quantification ◽  
Relative Standard

A simple and cost effective spectrophotometric method is described for the determination of fluvastatin sodium in pure form and in pharmaceutical formulations. When the drug reacts with sodium hydroxide shows absorption maximum at 304 nm and obeys beer's law in the concentration range 5-25 µg mL-1. The absorbance was found to increase linearly with increasing concentration of FVS, which is corroborated by the calculated correlation coefficient value of 0.9999 (n=5). The apparent molar absorptivity and sandell sensitivity were 1.1905×104and 0.0368844 µg cm-2cm respectively. The slope and intercept of the equation of the regression line are 0.027112 and 0.003539 respectively. The limit of detection and limit of quantification was found to be 0.0811 µg mL-1& 0.2460 µg mL-1. The validity of the described procedure was assessed. Statistical analysis of the result has been carried out revealing high accuracy and good precision. The proposed method was successfully applied to the determination of FVS in pharmaceutical formulations without any interference from common excipients. The relative standard deviations were ≤ 0.937%, with recoveries of 98.60% -101.70%.

A Simple and Effective Spectrophotometric Method for the Determination of Iron(III) with ResAcetophenone Guanylhydrazone (Rag)

2012 ◽  
Vol 3 (7) ◽  
pp. 54-55
Author(s):  
VITTHAL A DIVATE ◽  
◽  
PARAMANAND S MANOLI ◽  
SAVITA R DHONGADE
Keyword(s):  
Spectrophotometric Method
Sign in / Sign up

Export Citation Format

Share Document