Applying a Three-dimensional Habitat Volume Model to Estimate Sensitivity of Chesapeake Bay Living Resources to Environmental Change: a Proof-of-Concept Exercise

2021 ◽  
Author(s):  
Adam Schlenger ◽  
Elizabeth W. North ◽  
Yun Li ◽  
Ming Li ◽  
W. Michael Kemp
Keyword(s):  
Environmental Change ◽  
Chesapeake Bay ◽  
Three Dimensional ◽  
Proof Of Concept ◽  
Volume Model

scholarly journals A 3D hydrodynamic flow-focusing device for cell sorting

2021 ◽  
Vol 25 (3) ◽  
Author(s):  
Xiaofei Yuan ◽  
Andrew Glidle ◽  
Hitoshi Furusho ◽  
Huabing Yin
Keyword(s):  
Cell Sorting ◽  
Control Strategies ◽  
Three Dimensional ◽  
Hydrodynamic Flow ◽  
Proof Of Concept ◽  
Flow Focusing ◽  
Hydrodynamic Focusing ◽  
Cell Handling ◽  
Fluid Flow Control ◽  
High Degree

AbstractOptical-based microfluidic cell sorting has become increasingly attractive for applications in life and environmental sciences due to its ability of sophisticated cell handling in flow. The majority of these microfluidic cell sorting devices employ two-dimensional fluid flow control strategies, which lack the ability to manipulate the position of cells arbitrarily for precise optical detection, therefore resulting in reduced sorting accuracy and purity. Although three-dimensional (3D) hydrodynamic devices have better flow-focusing characteristics, most lack the flexibility to arbitrarily position the sample flow in each direction. Thus, there have been very few studies using 3D hydrodynamic flow focusing for sorting. Herein, we designed a 3D hydrodynamic focusing sorting platform based on independent sheath flow-focusing and pressure-actuated switching. This design offers many advantages in terms of reliable acquisition of weak Raman signals due to the ability to precisely control the speed and position of samples in 3D. With a proof-of-concept demonstration, we show this 3D hydrodynamic focusing-based sorting device has the potential to reach a high degree of accuracy for Raman activated sorting.

scholarly journals A Proof-of-Concept Algorithm for the Retrieval of Total Column Amount of Trace Gases in a Multi-Dimensional Atmosphere

2021 ◽  
Vol 13 (2) ◽  
pp. 270
Author(s):  
Adrian Doicu ◽  
Dmitry S. Efremenko ◽  
Thomas Trautmann
Keyword(s):  
Trace Gases ◽  
Three Dimensional ◽  
Principal Component ◽  
Radiative Transfer Model ◽  
Computational Time ◽  
Transfer Model ◽  
Proof Of Concept ◽  
Discrete Ordinate ◽  
Distribution Method ◽  
Total Column

An algorithm for the retrieval of total column amount of trace gases in a multi-dimensional atmosphere is designed. The algorithm uses (i) certain differential radiance models with internal and external closures as inversion models, (ii) the iteratively regularized Gauss–Newton method as a regularization tool, and (iii) the spherical harmonics discrete ordinate method (SHDOM) as linearized radiative transfer model. For efficiency reasons, SHDOM is equipped with a spectral acceleration approach that combines the correlated k-distribution method with the principal component analysis. The algorithm is used to retrieve the total column amount of nitrogen for two- and three-dimensional cloudy scenes. Although for three-dimensional geometries, the computational time is high, the main concepts of the algorithm are correct and the retrieval results are accurate.

Poly-(lactic acid) and fibrin bioactive cellularized scaffold for use in bone regenerative medicine: Proof of concept

2021 ◽  
pp. 088391152199640
Author(s):  
Renata Aquino de Carvalho ◽  
Valmir Vieira Rocha Júnior ◽  
Antonio José Felix Carvalho ◽  
Heloisa Sobreiro Selistre de Araújo ◽  
Mônica Rosas Costa Iemma ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Regenerative Medicine ◽  
Platelet Rich Plasma ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Infrapatellar Fat Pad ◽  
Dimensional Structure ◽  
Poly Lactic Acid ◽  
Proof Of Concept ◽  
Critical Bone Defects

Bone regenerative medicine (BRM) aims to overcome the limitations of conventional treatments for critical bone defects by developing therapeutic strategies, based on temporary bioactive substitutes, capable of stimulating, sustaining, and guiding tissue regeneration. The aim of this study was to validate the “proof of concept” of a cellularized bioactive scaffold and establish its potential for use in BRM. For this purpose, three-dimensional scaffolds of poly-(lactic acid) (PLA), produced by the additive manufacturing technique, were incorporated into a human platelet-rich plasma (PRP-h) fibrin matrix containing human infrapatellar fat pad mesenchymal stem cells (hIFPMSC). The scaffolds (PLA/finbrin-bioactive) were kept under ideal culture conditions in a medium free from fetal bovine serum and analyzed at 5 and 10 days by Scanning Electron Microscopy (SEM), Fourrier Transform Infrared (FTIR), Circular Dichroism and fluorescence microscopy. The results demonstrated the feasibility of obtaining a rigid, cytocompatible, and cellularized three-dimensional structure. In addition, PRP platelets and leukocytes were able to provide a bioactive environment capable of maintaining the viability of hIFPMSC into scaffolds. The results validate the concept of a customizable, bioactive, cellularized, and non-immunogenic strategy for application in BRM.

scholarly journals Challenges associated with modeling low-oxygen waters in Chesapeake Bay: a multiple model comparison

2016 ◽  
Vol 13 (7) ◽  
pp. 2011-2028 ◽  
Author(s):  
Isaac D. Irby ◽  
Marjorie A. M. Friedrichs ◽  
Carl T. Friedrichs ◽  
Aaron J. Bever ◽  
Raleigh R. Hood ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Chesapeake Bay ◽  
Mixed Layer ◽  
Model Comparison ◽  
Three Dimensional ◽  
Surface Mixed Layer ◽  
Multiple Model ◽  
Low Oxygen ◽  
Mixing Depth ◽  
Biogeochemical Models

Abstract. As three-dimensional (3-D) aquatic ecosystem models are used more frequently for operational water quality forecasts and ecological management decisions, it is important to understand the relative strengths and limitations of existing 3-D models of varying spatial resolution and biogeochemical complexity. To this end, 2-year simulations of the Chesapeake Bay from eight hydrodynamic-oxygen models have been statistically compared to each other and to historical monitoring data. Results show that although models have difficulty resolving the variables typically thought to be the main drivers of dissolved oxygen variability (stratification, nutrients, and chlorophyll), all eight models have significant skill in reproducing the mean and seasonal variability of dissolved oxygen. In addition, models with constant net respiration rates independent of nutrient supply and temperature reproduced observed dissolved oxygen concentrations about as well as much more complex, nutrient-dependent biogeochemical models. This finding has significant ramifications for short-term hypoxia forecasts in the Chesapeake Bay, which may be possible with very simple oxygen parameterizations, in contrast to the more complex full biogeochemical models required for scenario-based forecasting. However, models have difficulty simulating correct density and oxygen mixed layer depths, which are important ecologically in terms of habitat compression. Observations indicate a much stronger correlation between the depths of the top of the pycnocline and oxycline than between their maximum vertical gradients, highlighting the importance of the mixing depth in defining the region of aerobic habitat in the Chesapeake Bay when low-oxygen bottom waters are present. Improvement in hypoxia simulations will thus depend more on the ability of models to reproduce the correct mean and variability of the depth of the physically driven surface mixed layer than the precise magnitude of the vertical density gradient.

Fetal weight estimation by automated three-dimensional limb volume model in late third trimester compared to two-dimensional model: a cross-sectional prospective observational study

2021 ◽  
Author(s):  
Xining Wu ◽  
Zihan Niu ◽  
Zhonghui Xu ◽  
Yuxin Jiang ◽  
Yixiu Zhang ◽  
...  
Keyword(s):  
Birth Weight ◽  
3D Model ◽  
Three Dimensional ◽  
Fetal Weight ◽  
Percentage Error ◽  
Limb Volume ◽  
Third Trimester ◽  
Volume Model ◽  
Estimated Error ◽  
2D Model

Abstract Background: Accurate estimation of fetal weight is important for prenatal care and for detection of fetal growth abnormalities. Prediction of fetal weight entails the indirect measurement of fetal biometry by ultrasound that is then introduced into formulae to calculate the estimated fetal weight. The aim of our study was to evaluate the accuracy of the automated three-dimensional(3D) fractional limb volume model to predict fetal weight in the third trimester.Methods: Prospective 2D and 3D ultrasonography were performed among women with singleton pregnancies 7 days before delivery to obtain 2D data, including fetal biparietal diameter, abdominal circumference and femur length, as well as 3D data, including the fractional arm volume (AVol) and fractional thigh volume (TVol). The fetal weight was estimated using the 2D model and the 3D fractional limb volume model respectively. Percentage error = (estimated fetal weight - actual birth weight) ÷ actual birth weight × 100. Systematic errors (accuracy) were evaluated as the mean percentage error (MPE). Random errors (precision) were calculated as±1 SD of percentage error.Results: Ultrasound examination was performed on 56 fetuses at 39.6 ± 1.4 weeks gestation. The average birth weight of the newborns was 3393 ± 530 g. The average fetal weight estimated by the 2D model was 3478 ± 467 g, and the MPE was 3.2 ± 8.9. The average fetal weights estimated by AVol and TVol of the 3D model were 3268 ± 467 g and 3250 ± 485 g, respectively, and the MPEs were -3.3 ± 6.6 and -3.9 ± 6.1, respectively. For the 3D TVol model, the proportion of fetuses with estimated error ≤ 5% was significantly higher than that of the 2D model (55.4% vs. 33.9%, p < 0.05). For fetuses with a birth weight < 3500 g, the accuracy of the AVol and TVol models were better than the 2D model (-0.8 vs. 7.0 and -2.8 vs. 7.0, both p < 0.05). Moreover, for these fetus, the proportions of estimated error ≤ 5% of the AVol and TVol models were 58.1% and 64.5%, respectively, significantly higher than that of the 2D model (19.4%) (both p < 0.05). The consistency of different examiners measuring fetal AVol and TVol were satisfactory,with the intraclass correlation coefficients of 0.921 and 0.963, respectively.Conclusion: In this cohort,the automated 3D fractional limb volume model improves the accuracy of weight estimation in most third-trimester fetuses. In particular, the 3D model estimation accuracy for fetuses with weight < 3500 g is significantly higher than that of the traditional 2D model.

scholarly journals Multifunctional Operation of an Organic Device with Three-Dimensional Architecture

Materials ◽  
2019 ◽  
Vol 12 (8) ◽  
pp. 1357
Author(s):  
Giuseppe Tarabella ◽  
Simone Luigi Marasso ◽  
Valentina Bertana ◽  
Davide Vurro ◽  
Pasquale D’Angelo ◽  
...  
Keyword(s):  
Selective Laser Sintering ◽  
Three Dimensional ◽  
Organic Thin Films ◽  
Dip Coating ◽  
Proof Of Concept ◽  
Polystyrene Sulfonate ◽  
Humidity Sensors ◽  
Three Dimensional Objects ◽  
Terminal Device ◽  
Dip Coating Technique

This work aims to show the feasibility of an innovative approach for the manufacturing of organic-based devices with a true three-dimensional and customizable structure that is made possible by plastic templates, fabricated by additive manufacturing methods, and coated by conducting organic thin films. Specifically, a three-dimensional prototype based on a polyamide structure covered by poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) using the dip-coating technique demonstrated a multifunctional character. The prototype is indeed able to operate both as a three-terminal device showing the typical response of organic electrochemical transistors (OECTs), with a higher amplification performance with respect to planar (2D) all-PEDOT:PSS OECTs, and as a two-terminal device able to efficiently implement a resistive sensing of water vaporization and perspiration, showing performances at least comparable to that of state-of-art resistive humidity sensors based on pristine PEDOT:PSS. To our knowledge, this is the first reported proof-of-concept of a true 3D structured OECT, obtained by exploiting a Selective laser sintering approach that, though simple in terms of 3D layout, paves the way for the integration of sensors based on OECTs into three-dimensional objects in various application areas.

scholarly journals A Novel Method for Electrospinning Nanofibrous 3-D Structures

Fibers ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 27 ◽  
Author(s):  
Daniela Lubasova ◽  
Anil N. Netravali
Keyword(s):  
Three Dimensional ◽  
Development Trend ◽  
Proof Of Concept ◽  
Structural Variations ◽  
Fiber Volume ◽  
Electrospinning Technique ◽  
Web Structure ◽  
Fiber Spacing ◽  
Novel Method ◽  
Volume Unit

The fast and precise fabrication of three-dimensional (3-D) structures made of nanofibers is an important development trend in the electrospinning technique. This paper describes a new and facile method of electrospinning to fabricate nanofibrous 3-D structures. The nanofibrous 3-D structures can be engineered to have the desired layer thicknesses, where the fiber spacing, density (i.e., fiber volume/unit volume), as well as shape of the structure may be controlled. While innumerable structural variations are possible with this method, this paper discusses, as proof-of-concept, a few cases that illustrate how 3-D nanofiber webs can be made for filtration application. Computerized automation of the method will make it possible to build almost any 3-D web structure suitable for a myriad of applications including ultra-light-weight insulation and scaffolds for hydrogel preparation and tissue.

scholarly journals Beyond the lognormal approximation: a general simulation scheme

2020 ◽  
Vol 498 (2) ◽  
pp. 2663-2675
Author(s):  
Federico Tosone ◽  
Mark C Neyrinck ◽  
Benjamin R Granett ◽  
Luigi Guzzo ◽  
Nicola Vittorio
Keyword(s):  
Correlation Function ◽  
Random Fields ◽  
Three Dimensional ◽  
Analytical Techniques ◽  
Proof Of Concept ◽  
Matter Distribution ◽  
Modest Improvement ◽  
Local Properties ◽  
Non Local ◽  
Simulation Scheme

ABSTRACT We present a public code to generate random fields with an arbitrary probability distribution function (PDF) and an arbitrary correlation function. The algorithm is cosmology independent and applicable to any stationary stochastic process over a three-dimensional grid. We implement it in the case of the matter density field, showing its benefits over the lognormal approximation, which is often used in cosmology for the generation of mock catalogues. We find that the covariance of the power spectrum from the new fast realizations is more accurate than that from a lognormal model. As a proof of concept, we also apply the new simulation scheme to the divergence of the Lagrangian displacement field. We find that information from the correlation function and the PDF of the displacement–divergence provides modest improvement over other standard analytical techniques to describe the particle field in the simulation. This suggests that further progress in this direction should come from multiscale or non-local properties of the initial matter distribution.

The Digital Revolution to Come: Photogrammetry in Archaeological Practice

2020 ◽  
Vol 85 (4) ◽  
pp. 737-760 ◽  
Author(s):  
Matthew Magnani ◽  
Matthew Douglass ◽  
Whittaker Schroder ◽  
Jonathan Reeves ◽  
David R. Braun
Keyword(s):  
Three Dimensional ◽  
Proof Of Concept ◽  
Digital Revolution ◽  
Archaeological Practice ◽  
3D Data ◽  
Analytical Potential ◽  
Visual Aid ◽  
To Come ◽  
Archaeological Analysis

The three-dimensional (3D) revolution promised to transform archaeological practice. Of the technologies that contribute to the proliferation of 3D data, photogrammetry facilitates the rapid and inexpensive digitization of complex subjects in both field and lab settings. It finds additional use as a tool for public outreach, where it engages audiences ranging from source communities to artifact collectors. But what has photogrammetry's function been in advancing archaeological analysis? Drawing on our previous work, we review recent applications to understand the role of photogrammetry for contemporary archaeologists. Although photogrammetry is widely used as a visual aid, its analytical potential remains underdeveloped. Considering various scales of inquiry—graduating from objects to landscapes—we address how the technology fits within and expands existing documentation and data visualization routines, while evaluating the opportunity it presents for addressing archaeological questions and problems in innovative ways. We advance an agenda advocating that archaeologists move from proof-of-concept papers toward greater integration of photogrammetry with research.

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