Muscle-derived stem cells differentiate into functional smooth muscle cells for ureter tissue engineering: An experimental study

2012 ◽  
Vol 17 (3) ◽  
pp. 456-464 ◽  
Author(s):  
Zhan-Kui Zhao ◽  
Hong-Lian Yu ◽  
Fei Xiao ◽  
Shi-Wen Li ◽  
Wen-Biao Liao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Experimental Study ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells

scholarly journals Urine-Derived Stem Cells: Differentiation Potential into Smooth-Muscle Cells and Urothelial Cell

2019 ◽  
Vol 74 (3) ◽  
pp. 176-184
Author(s):  
Igor A. Vasyutin ◽  
Aleksey V. Lyundup ◽  
Sergey L. Kuznetsov
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Urinary Tract ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Proliferative Potential ◽  
Differentiation Potential ◽  
Invasive Approach

Background: Tissue engineering of low urinary tract organs requires biopsy of urinary bladder material. The current study describes non-invasive approach of obtaining autologous stem cells from urine of healthy adults. These cells were studied for potential to differentiate into epithelial cells and smooth muscle cells of the urinary bladder. Aims: To describe properties of urine-derived stem cells (USCs) and investigate their differentiation potential for tissue engineering of low urinary tract organs. Materials and Methods: USCs were isolated from urine of healthy volunteers with centrifugation and seeded in media to 24-well plates. Expression of stem cells markers (CD73, CD90, CD105, CD34, CD45, CD29, CD44, CD54, SSEA4) by USCs was assessed with flow cytometry. Expression of specific markers of smooth muscle cells and urothelial cells was assessed with fluorescence microscopy with following computational image analysis. Results: Median number of USCs per 100 ml urine was 6. Doubling time for USC was 1.440.528 days (n=4) and there were 26.34.79 population doublings for USC cultures (n=4). Median expression of markers of postnatal stem cells was CD73 ― 79.8%, CD90 ― 56.6%, CD105 ― 40.7%, CD34 1.0%, CD45 2.0%, CD29 99.0%, CD44 99.0%, CD54 ― 97.7% and SSEA4 99.0%. Treatment of cells with high concentration of EGF in media with low concentration of FBS for 10 days increased cytokeratin (CK) expression to 24.9% for CK AE1/AE3 and to 7.6% for CK 7. Treatment of USCs with media inducing smooth muscle differentiation for 10 days increased expression of -smooth muscle actin to 79.6% and expression of calponin to 97.6%. Conclusions: USCs are cells that can be found in urine in small quantities. They have high proliferative potential and express markers of postnatal stem cells. Under effect of PDGF-BB and TGF- 1 they differentiate into smooth muscle cells.

scholarly journals Differentiation of Adipose-Derived Stem Cells into Vascular Smooth Muscle Cells for Tissue Engineering Applications

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 797
Author(s):  
Alvaro Yogi ◽  
Marina Rukhlova ◽  
Claudie Charlebois ◽  
Ganghong Tian ◽  
Danica B. Stanimirovic ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Neointimal Hyperplasia ◽  
Muscle Cells ◽  
Regular Growth ◽  
Growth Media ◽  
Adipose Derived Stem Cells ◽  
Vascular Bypass

Synthetic grafts have been developed for vascular bypass surgery, however, the risks of thrombosis and neointimal hyperplasia still limit their use. Tissue engineering with the use of adipose-derived stem cells (ASCs) has shown promise in addressing these limitations. Here we further characterized and optimized the ASC differentiation into smooth muscle cells (VSMCs) induced by TGF-β and BMP-4. TGF-β and BMP-4 induced a time-dependent expression of SMC markers in ASC. Shortening the differentiation period from 7 to 4 days did not impair the functional property of contraction in these cells. Stability of the process was demonstrated by switching cells to regular growth media for up to 14 days. The role of IGFBP7, a downstream effector of TGF-β, was also examined. Finally, topographic and surface patterning of a substrate is recognized as a powerful tool for regulating cell differentiation. Here we provide evidence that a non-woven PET structure does not affect the differentiation of ASC. Taken together, our results indicate that VSMCs differentiated from ASCs are a suitable candidate to populate a PET-based vascular scaffolds. By employing an autologous source of cells we provide a novel alternative to address major issues that reduces long-term patency of currently vascular grafts.

The role of adipose derived stem cells, smooth muscle cells and low intensity laser irradiation (LILI) in tissue engineering and regenerative medicine

2013 ◽  
Vol 8 (4) ◽  
pp. 331-336
Author(s):  
Bernard Mvula ◽  
Heidi Abrahamse
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Regenerative Medicine ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells ◽  
Low Intensity ◽  
Intensity Laser

AbstractTissue engineering and regenerative medicine has become the treatment of choice for several degenerative diseases. It involves the repairing or replacing of diseased or damaged cells or tissues. Stem cells have a key role to play in this multidisciplinary science because of their capacity to differentiate into several lineages. Adipose derived stem cells (ADSCs) are adult mesenchymal stem cells that are easily harvested and have the capacity to differentiate into cartilage, bone, smooth muscle, fat, liver and nerve cells. ADSCs have been found to differentiate into smooth muscle cells which play major roles in diseases such as asthma, hypertension, cancer and arteriosclerosis. Low Intensity Laser Irradiation (LILI), which involves the application of monochromatic light, has been found to increase viability, proliferation and differentiation in several types of cells including ADSCs. This review discusses the role of ADSCs, smooth muscle cells and LILI in the science of tissue engineering and regenerative medicine.

scholarly journals Human-Induced Pluripotent Stem-Cell-Derived Smooth Muscle Cells Increase Angiogenesis to Treat Hindlimb Ischemia

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 792
Author(s):  
Xixiang Gao ◽  
Mingjie Gao ◽  
Jolanta Gorecka ◽  
John Langford ◽  
Jia Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Stem Cell ◽  
Smooth Muscle Cells ◽  
Functional Outcomes ◽  
Ethical Issues ◽  
Muscle Cells ◽  
Doppler Imaging ◽  
Hindlimb Ischemia ◽  
Induced Pluripotent

Induced pluripotent stem cells (iPSC) represent an innovative, somatic cell-derived, easily obtained and renewable stem cell source without considerable ethical issues. iPSC and their derived cells may have enhanced therapeutic and translational potential compared with other stem cells. We previously showed that human iPSC-derived smooth muscle cells (hiPSC-SMC) promote angiogenesis and wound healing. Accordingly, we hypothesized that hiPSC-SMC may be a novel treatment for human patients with chronic limb-threatening ischemia who have no standard options for therapy. We determined the angiogenic potential of hiPSC-SMC in a murine hindlimb ischemia model. hiPSC-SMC were injected intramuscularly into nude mice after creation of hindlimb ischemia. Functional outcomes and perfusion were measured using standardized scores, laser Doppler imaging, microCT, histology and immunofluorescence. Functional outcomes and blood flow were improved in hiPSC-SMC-treated mice compared with controls (Tarlov score, p < 0.05; Faber score, p < 0.05; flow, p = 0.054). hiPSC-SMC-treated mice showed fewer gastrocnemius fibers (p < 0.0001), increased fiber area (p < 0.0001), and enhanced capillary density (p < 0.01); microCT showed more arterioles (<96 μm). hiPSC-SMC treatment was associated with fewer numbers of macrophages, decreased numbers of M1-type (p < 0.05) and increased numbers of M2-type macrophages (p < 0.0001). Vascular endothelial growth factor (VEGF) expression in ischemic limbs was significantly elevated with hiPSC-SMC treatment (p < 0.05), and inhibition of VEGFR-2 with SU5416 was associated with fewer capillaries in hiPSC-SMC-treated limbs (p < 0.0001). hiPSC-SMC promote VEGF-mediated angiogenesis, leading to improved hindlimb ischemia. Stem cell therapy using iPSC-derived cells may represent a novel and potentially translatable therapy for limb-threatening ischemia.

scholarly journals MicroRNA-145 regulates the differentiation of human adipose-derived stem cells to smooth muscle cells via targeting Krüppel-like factor 4

2017 ◽  
Vol 15 (6) ◽  
pp. 3787-3795 ◽  
Author(s):  
Kaisaier Aji ◽  
Yun Zhang ◽  
Abudusaimi Aimaiti ◽  
Yujie Wang ◽  
Mulati Rexiati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells

Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils

2021 ◽  
Vol 16 (2) ◽  
pp. 025016
Author(s):  
Martina Travnickova ◽  
Nikola Slepickova Kasalkova ◽  
Antonin Sedlar ◽  
Martin Molitor ◽  
Jana Musilkova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells
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